##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	SRR5665179
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2856312
Total Bases	707.5 Mbp
Sequences flagged as poor quality	0
Sequence length	75-252
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	28.592301191186397	32.0	25.0	33.0	18.0	33.0
2	30.146918823994017	31.0	29.0	33.0	25.0	33.0
3	31.29747765650251	33.0	31.0	33.0	27.0	34.0
4	30.720660067947758	33.0	31.0	33.0	27.0	33.0
5	31.387683488358416	33.0	31.0	33.0	29.0	34.0
6	34.67120398611917	37.0	34.0	38.0	28.0	38.0
7	35.91313413940774	38.0	36.0	38.0	31.0	38.0
8	35.76124842104084	38.0	36.0	38.0	31.0	38.0
9	36.36958322480177	38.0	37.0	38.0	33.0	38.0
10-14	36.66863500906063	38.0	37.8	38.0	34.0	38.0
15-19	36.69601752189537	38.0	38.0	38.0	34.0	38.0
20-24	36.71220503922541	38.0	38.0	38.0	34.0	38.0
25-29	36.65960091194519	38.0	38.0	38.0	34.0	38.0
30-34	36.62837022005999	38.0	38.0	38.0	34.0	38.0
35-39	36.59175832332043	38.0	38.0	38.0	34.0	38.0
40-44	36.548934010010115	38.0	38.0	38.0	34.0	38.0
45-49	36.52149674125236	38.0	38.0	38.0	34.0	38.0
50-54	36.46122874531913	38.0	38.0	38.0	34.0	38.0
55-59	36.372144149518675	38.0	37.0	38.0	33.6	38.0
60-64	36.314701125087176	38.0	37.0	38.0	33.0	38.0
65-69	36.23268732547425	38.0	37.0	38.0	33.0	38.0
70-74	36.14098872952255	38.0	37.0	38.0	33.0	38.0
75-79	36.010888281302755	38.0	37.0	38.0	31.8	38.0
80-84	35.856275789600716	38.0	37.0	38.0	31.0	38.0
85-89	35.69423952488511	38.0	36.2	38.0	30.6	38.0
90-94	35.566856836685915	38.0	36.0	38.0	29.0	38.0
95-99	35.426590439058494	38.0	36.0	38.0	29.0	38.0
100-104	35.269736341294475	38.0	35.6	38.0	28.0	38.0
105-109	35.05212045644097	38.0	35.0	38.0	27.6	38.0
110-114	34.86224716651772	38.0	35.0	38.0	27.0	38.0
115-119	34.68283862224111	38.0	34.0	38.0	26.2	38.0
120-124	34.77973727899684	38.0	34.0	38.0	27.4	38.0
125-129	33.658267334183655	34.2	33.2	35.6	31.0	36.2
130-134	35.23326595067307	36.2	36.0	37.2	32.8	38.0
135-139	36.406185164732996	38.0	38.0	38.0	34.0	38.0
140-144	36.351464269816134	38.0	38.0	38.0	33.0	38.0
145-149	36.32655646901712	38.0	38.0	38.0	33.0	38.0
150-154	36.30457813875516	38.0	38.0	38.0	33.0	38.0
155-159	36.29334673688937	38.0	38.0	38.0	33.0	38.0
160-164	36.27255645292395	38.0	38.0	38.0	33.0	38.0
165-169	36.212825471075824	38.0	37.2	38.0	33.0	38.0
170-174	36.16327693974875	38.0	37.0	38.0	33.0	38.0
175-179	36.09635567318176	38.0	37.0	38.0	33.0	38.0
180-184	36.00932282906693	38.0	37.0	38.0	31.2	38.0
185-189	35.88513607449757	38.0	37.0	38.0	31.0	38.0
190-194	35.76733905397485	38.0	37.0	38.0	30.2	38.0
195-199	35.6328854925164	38.0	37.0	38.0	29.0	38.0
200-204	35.44895306788584	38.0	36.2	38.0	29.0	38.0
205-209	35.26594414373988	38.0	36.0	38.0	28.0	38.0
210-214	35.074532213808766	38.0	36.0	38.0	27.0	38.0
215-219	34.791438561365574	38.0	35.0	38.0	25.4	38.0
220-224	34.59107906342822	38.0	35.0	38.0	23.8	38.0
225-229	34.36186929485922	38.0	34.0	38.0	23.0	38.0
230-234	34.10463261657729	38.0	34.0	38.0	23.0	38.0
235-239	33.86838511850907	38.0	33.6	38.0	21.8	38.0
240-244	33.72102941814116	38.0	33.0	38.0	21.0	38.0
245-249	34.00470810347685	38.0	33.0	38.0	23.0	38.0
250-252	35.44828323108447	37.666666666666664	33.666666666666664	38.0	30.333333333333332	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
15	1.0
16	5.0
17	14.0
18	28.0
19	61.0
20	179.0
21	689.0
22	2010.0
23	4565.0
24	8518.0
25	14131.0
26	20895.0
27	29669.0
28	39582.0
29	51168.0
30	65607.0
31	83560.0
32	108097.0
33	144426.0
34	202616.0
35	317891.0
36	603685.0
37	1158915.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	46.09860275638314	19.646339345001778	12.397139951305206	21.85791794730988
2	16.032321478273502	21.295134798279598	40.86121755204714	21.811326171399763
3	18.023832872306794	29.97594446255252	29.673035846636935	22.32718681850375
4	13.568593236989646	22.117164081389156	37.28814390074999	27.02609878087121
5	16.73204252768613	35.59687624286491	33.78684481763845	13.884236411810507
6	28.502443196598115	37.65204553164777	20.427203174457574	13.418308097296535
7	24.055005195510855	31.28418744170805	26.526233828797412	18.134573533983684
8	23.001004792897128	34.7797332922547	22.825113520591252	19.394148394256927
9	23.080723030763465	18.09141864853605	21.531451418088373	37.29640690261211
10-14	23.565509202010666	23.887206363726246	29.149503369547226	23.397781064715865
15-19	22.79090118942897	28.6778705322017	26.463182345848935	22.06804593252039
20-24	22.620223879170478	27.508589232970138	27.31162172042615	22.559565167433238
25-29	22.792270043614625	27.43949238506881	27.094388791320966	22.673848779995602
30-34	22.62547407682154	27.297245878263393	27.330555457718926	22.74672458719614
35-39	22.461072005629788	26.8674181221083	27.721346457836976	22.950163414424935
40-44	22.07407553688234	26.44524775960993	28.209072400675883	23.271604302831843
45-49	21.364322129707727	26.360441002489733	28.775443966291437	23.499792901511103
50-54	20.82924381951546	26.490841788068366	28.585944909636247	24.09396948277993
55-59	20.52037087023848	26.168772356212163	28.339559900556978	24.971296872992383
60-64	20.68647165843542	26.12515265323773	27.52372623953799	25.66464944878886
65-69	20.634975539783547	26.071175147697474	26.88129684450394	26.412552468015043
70-74	20.717067144153127	25.966372326847438	26.475193138624842	26.841367390374593
75-79	20.743110927126928	25.696462557892524	26.39871060449797	27.161715910482577
80-84	20.692446440509126	25.41907286673213	26.459455994259223	27.42902469849952
85-89	20.736293734342578	24.800774019996684	26.829481439653662	27.63345080600707
90-94	20.828636798573	24.12466582707938	27.119329984694712	27.927367389652908
95-99	20.61030112044818	23.788375350140058	27.65435574229692	27.946967787114847
100-104	20.50660356415286	24.17469629347431	27.703567584374984	27.615132557997846
105-109	20.137729349019523	25.678104518516324	27.26727907880865	26.916887053655504
110-114	19.803912517488047	27.086414368998874	26.75379035244655	26.35588276106653
115-119	19.71930408184971	27.622118972313242	26.59480775679382	26.063769189043228
120-124	19.897951029952306	27.822990352345467	26.485916835591745	25.793141782110485
125-129	22.308846401832206	25.902649889633356	28.005598127561655	23.78290558097278
130-134	21.768328176392725	30.818294056623603	27.522510313411637	19.89086745357204
135-139	23.592156315825246	23.699720119981784	28.329598430108007	24.378525134084963
140-144	22.41493926391491	27.429777973683212	26.989237269046296	23.16604549335558
145-149	22.2845799845717	27.26720765261647	27.672721719810152	22.775490643001678
150-154	22.306604933458456	27.05870395340046	27.685319542465386	22.949371570675698
155-159	22.11768131576866	26.85509903461998	27.79385410102142	23.233365548589944
160-164	21.936405733409988	26.773792556856467	27.99841882822831	23.291382881505236
165-169	21.81700782401096	26.603250277205166	27.86754671704703	23.712195181736845
170-174	21.725533680049917	26.630103033063364	27.853723676642105	23.790639610244618
175-179	21.753088993876606	26.127982012303146	27.81530067193233	24.303628321887917
180-184	21.805380707305755	25.842922367095312	27.722224347287405	24.62947257831153
185-189	21.356932519149492	25.167119295000223	28.649080372744347	24.826867813105935
190-194	21.231253486973674	25.507842176488815	28.13176564492965	25.129138691607857
195-199	21.04060098482377	25.24238744309873	28.627554385324615	25.089457186752885
200-204	21.577460540987133	24.877140549574968	27.97560309695319	25.569795812484706
205-209	21.804870869389706	24.373478612508734	28.23639391933712	25.585256598764445
210-214	21.905285318973792	24.622686089582203	27.44775225997505	26.024276331468954
215-219	21.440038170228203	24.753828424511166	27.517064649116435	26.289068756144196
220-224	21.57090344631804	24.681232311880958	28.177348287904003	25.570515953897
225-229	21.265540194928487	25.412120008454114	27.694977377709034	25.627362418908362
230-234	21.64308903883854	25.602238177858798	27.618859319559892	25.135813463742775
235-239	21.93891210074598	25.219371172198823	27.61009946896518	25.23161725809001
240-244	21.744100752010535	25.064363899501696	27.797667266953642	25.393868081534134
245-249	21.511094020067596	26.036535837348374	27.468711756657992	24.983658385926034
250-252	20.22447979237379	26.556075087529546	27.814166966080062	25.405278154016603
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.3333333333333333
14	1.3333333333333333
15	1.3333333333333333
16	3.3333333333333335
17	8.33333333333333
18	14.33333333333334
19	28.49999999999997
20	53.33333333333342
21	84.33333333333323
22	144.83333333333303
23	217.16666666666836
24	352.9999999999977
25	531.4999999999899
26	837.5000000000244
27	1239.0000000000007
28	1849.4999999998633
29	2686.333333333425
30	3866.333333333957
31	5508.999999999505
32	7690.666666664208
33	10447.16666666762
34	14230.166666674446
35	19091.666666669295
36	24938.333333314826
37	32080.33333328909
38	40615.500000006985
39	49868.66666674056
40	59944.66666681328
41	69851.50000013622
42	79436.16666666443
43	87516.83333321469
44	93041.33333313577
45	95993.8333330943
46	95717.3333330993
47	91940.49999982124
48	84803.33333325843
49	76287.00000004856
50	66379.00000018947
51	56608.83333345239
52	47489.16666672009
53	39057.33333332651
54	32082.666666623758
55	26264.66666664454
56	21606.166666661196
57	17772.50000000816
58	14700.833333341601
59	12530.500000004435
60	10613.000000001013
61	9113.333333331691
62	7926.333333330749
63	6768.16666666511
64	5777.333333332657
65	4750.833333333581
66	3825.833333333911
67	3018.1666666668857
68	2342.833333333254
69	1736.4999999998975
70	1248.1666666666722
71	867.5000000000252
72	584.8333333333275
73	410.6666666666618
74	275.6666666666692
75	192.66666666666754
76	120.49999999999945
77	70.00000000000011
78	37.333333333333314
79	25.499999999999986
80	13.833333333333336
81	6.0
82	4.166666666666666
83	3.6666666666666665
84	2.1666666666666665
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.5
91	0.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.03416993661756839
2	2.450712667243635E-4
3	1.7505090480311673E-4
4	3.5010180960623347E-4
5	2.800814476849868E-4
6	4.5513235248810356E-4
7	0.0
8	7.00203619212467E-5
9	7.00203619212467E-5
10-14	8.122361982864616E-4
15-19	7.002036192124669E-6
20-24	1.190346152661194E-4
25-29	0.0023876943415145125
30-34	0.002681779861583749
35-39	0.0026957839339679978
40-44	0.010986194785443607
45-49	0.006406863115794073
50-54	8.05234162094337E-4
55-59	1.0503054288187005E-4
60-64	6.301832572912203E-5
65-69	1.4004072384249338E-5
70-74	7.562199087494644E-4
75-79	5.181530364993301E-4
80-84	0.002163668876596511
85-89	0.0018486058990694791
90-94	0.004824686372627825
95-99	0.0022898635304359626
100-104	0.0016176877275836277
105-109	9.384787273836256E-4
110-114	4.132741412688693E-4
115-119	0.0010579601729141317
120-124	8.691505119156329E-4
125-129	0.0018146179290300115
130-134	0.0033245242831142336
135-139	0.0025708715324929987
140-144	0.0013664358282291557
145-149	0.0016340882069544541
150-154	0.0020778282623523723
155-159	0.002056700268124689
160-164	0.0025286275504184737
165-169	0.0021975888787352143
170-174	0.0029583252272874228
175-179	0.004226253911926277
180-184	0.005268944762087773
185-189	0.003860500939447268
190-194	0.0027408852354712824
195-199	0.001599972934378555
200-204	0.0015728132112924323
205-209	0.0020903854416518338
210-214	0.00495607290977979
215-219	0.013196471341817208
220-224	0.015015942259690118
225-229	0.016258055975364227
230-234	0.03161251781982442
235-239	0.07828483785011749
240-244	0.03237875840307743
245-249	1.781099179065944E-4
250-252	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
75-79	35.0
80-84	49.0
85-89	55.0
90-94	76.0
95-99	100.0
100-104	191.0
105-109	337.0
110-114	565.0
115-119	905.0
120-124	2427.0
125-129	12068.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150-154	1.0
155-159	14.0
160-164	9.0
165-169	22.0
170-174	40.0
175-179	75.0
180-184	197.0
185-189	390.0
190-194	730.0
195-199	1348.0
200-204	2906.0
205-209	5063.0
210-214	11102.0
215-219	16721.0
220-224	24956.0
225-229	39387.0
230-234	63199.0
235-239	81253.0
240-244	130903.0
245-249	384033.0
250-253	2077155.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	93.65518110462689
#Duplication Level	Percentage of total
1	88.28273437323537
2	9.357693755549954
3	1.6136514686738976
4	0.4163143763646597
5	0.14441468109776204
6	0.05768443826991416
7	0.03543169237113133
8	0.018020836982725445
9	0.010310139058330822
>10	0.06133673056070245
>50	0.002407507835577377
>100	0.0
>500	0.0
>1k	0.0
>5k	0.0
>10k+	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	0.0	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0	0.0
5	0.0	3.501018096062335E-5	0.0	3.501018096062335E-5	0.0	0.0
6	3.501018096062335E-5	3.501018096062335E-5	3.501018096062335E-5	7.00203619212467E-5	0.0	0.0
7	3.501018096062335E-5	3.501018096062335E-5	3.501018096062335E-5	1.0503054288187005E-4	3.501018096062335E-5	0.0
8	3.501018096062335E-5	7.00203619212467E-5	3.501018096062335E-5	1.400407238424934E-4	3.501018096062335E-5	0.0
9	3.501018096062335E-5	7.00203619212467E-5	3.501018096062335E-5	2.100610857637401E-4	3.501018096062335E-5	0.0
10-14	3.501018096062335E-5	8.402443430549603E-5	9.802850668974537E-5	2.5207330291648813E-4	4.9014253344872684E-5	0.0
15-19	7.00203619212467E-5	1.1203257907399472E-4	1.2603665145824406E-4	0.0010012911754738278	1.0503054288187005E-4	0.0
20-24	1.190346152661194E-4	1.820529409952414E-4	1.400407238424934E-4	0.006637930310134187	1.4704276003461807E-4	0.0
25-29	1.400407238424934E-4	2.1706312195586474E-4	1.4704276003461807E-4	0.0936382299972832	1.8905497718736607E-4	0.0
30-34	1.400407238424934E-4	3.4309977341410883E-4	1.7505090480311673E-4	0.8136226014525023	5.811690039463477E-4	2.800814476849868E-5
35-39	2.100610857637401E-4	4.201221715274802E-4	1.820529409952414E-4	2.453751550951017	7.212097277888409E-4	3.501018096062335E-5
40-44	2.5207330291648813E-4	4.4813031629597886E-4	2.1706312195586474E-4	5.508725937502627	8.192382344785863E-4	3.501018096062335E-5
45-49	2.800814476849868E-4	4.5513235248810356E-4	2.730794114928621E-4	8.914789420763558	9.732830307053293E-4	3.501018096062335E-5
50-54	2.800814476849868E-4	4.901425334487269E-4	3.220936648377348E-4	12.429692554594876	0.0012813726231588147	3.501018096062335E-5
55-59	2.800814476849868E-4	4.901425334487269E-4	3.5010180960623347E-4	15.901519161772246	0.001890549771873661	3.501018096062335E-5
60-64	3.010875562613608E-4	6.301832572912202E-4	3.6410588199048286E-4	19.35099526942435	0.0024507126672436348	3.501018096062335E-5
65-69	3.5010180960623347E-4	7.002036192124669E-4	4.0611809914323086E-4	22.685364904114113	0.003010875562613608	3.501018096062335E-5
70-74	3.5010180960623347E-4	7.212097277888409E-4	4.4813031629597886E-4	25.963550200398277	0.0033679794084119663	3.501018096062335E-5
75-79	3.781099543747322E-4	7.492178725573397E-4	5.11148642025101E-4	29.138392444522868	0.003781099543747322	3.501018096062335E-5
80-84	4.131201353353555E-4	7.702239811337137E-4	5.251527144093502E-4	32.25032139346122	0.0042292298600433015	3.501018096062335E-5
85-89	4.5513235248810356E-4	7.982321259022123E-4	5.251527144093502E-4	35.25548329454206	0.0050694742030982605	3.501018096062335E-5
90-94	4.901425334487269E-4	8.402443430549604E-4	5.251527144093502E-4	38.17590655362579	0.009900879175664283	3.501018096062335E-5
95-99	4.901425334487269E-4	9.382728497447058E-4	5.531608591778489E-4	40.98346399132868	0.06260520559378667	3.501018096062335E-5
100-104	5.181506782172256E-4	9.662809945132046E-4	6.091771487148463E-4	43.68389727732825	0.1889149364635236	3.501018096062335E-5
105-109	5.531608591778489E-4	0.001043303392626576	6.651934382518437E-4	46.28804556364991	0.4089189136200807	3.501018096062335E-5
110-114	5.601628953699736E-4	0.0010503054288187005	6.651934382518437E-4	48.763825520461346	0.6139735435064517	3.501018096062335E-5
115-119	5.881710401384723E-4	0.0010713115373950745	6.651934382518437E-4	50.17084968308784	0.7632079408692047	3.501018096062335E-5
120-124	6.161791849069709E-4	0.001085315609779324	6.651934382518437E-4	50.20230282966286	0.7837099028397458	3.501018096062335E-5
125-129	6.581914020597191E-4	0.001085315609779324	6.651934382518437E-4	50.20297502513731	0.78382193541882	5.601628953699736E-5
130-134	6.721954744439683E-4	0.0011133237545478225	6.79197510636093E-4	50.20450146902719	0.7838499435635884	9.102647049762071E-5
135-139	7.142076915967163E-4	0.0011203257907399472	7.352138001730903E-4	50.207232263142124	0.7838919557807411	1.400407238424934E-4
140-144	7.352138001730903E-4	0.0011483339355084457	7.352138001730903E-4	50.211384470604045	0.784003988359815	1.6804886861099207E-4
145-149	7.702239811337137E-4	0.0011553359717005704	7.42215836365215E-4	50.21745523598263	0.7840950148303126	1.9605701337949074E-4
150-154	7.702239811337137E-4	0.0011553359717005704	7.842280535179629E-4	50.22680995633531	0.7841440290836577	2.100610857637401E-4
155-159	8.472463792470851E-4	0.0011763420802769445	8.612504516313345E-4	50.23951164998782	0.7841580331560418	2.380692305322388E-4
160-164	9.452748859368305E-4	0.001190346152661194	9.102647049762071E-4	50.25393584454359	0.7841930433370023	2.800814476849868E-4
165-169	0.0010012911754738278	0.001190346152661194	9.172667411683318E-4	50.2699354972426	0.7843120779522685	2.800814476849868E-4
170-174	0.0010292993202423266	0.001190346152661194	9.592789583210799E-4	50.28684541464658	0.784396102386574	2.800814476849868E-4
175-179	0.0010783135735871992	0.001253364478390316	9.802850668974537E-4	50.30741739697904	0.784571153291377	2.800814476849868E-4
180-184	0.0011203257907399472	0.0012603665145824406	0.0010152952478580772	50.33305885351461	0.7847111940152196	3.0808959245348547E-4
185-189	0.0011553359717005704	0.00127437058696669	0.0010152952478580772	50.361445108237476	0.7850332876800574	3.5010180960623347E-4
190-194	0.0011553359717005704	0.001295376695543064	0.0010503054288187007	50.388704035133415	0.7857054831545013	3.8511199056685683E-4
195-199	0.0012253563336218172	0.0013023787317351886	0.0010923176459714484	50.41698525931341	0.7877710838311781	3.8511199056685683E-4
200-204	0.0012393604060060666	0.0013303868765036874	0.0011203257907399472	50.44610672783646	0.7958654376692742	3.8511199056685683E-4
205-209	0.0012603665145824406	0.001358395021272186	0.0011203257907399472	50.471328062200485	0.8153030901386125	3.8511199056685683E-4
210-214	0.001358395021272186	0.0014774296365383053	0.0011203257907399472	50.497158573713236	0.8404123919235713	3.8511199056685683E-4
215-219	0.0014004072384249339	0.001505437781306804	0.0011553359717005704	50.53129350014984	0.8729368500359905	3.8511199056685683E-4
220-224	0.0014074092746170586	0.0015334459260753028	0.0011833441164690692	50.56617064242282	0.9152291486364235	4.131201353353555E-4
225-229	0.0014354174193855573	0.0015684561070359259	0.001190346152661194	50.598148941712246	0.959797108999297	4.201221715274802E-4
230-234	0.001463425564154056	0.0016314744327650481	0.0011973481888533186	50.63061738353514	0.9933228582871898	4.201221715274802E-4
235-239	0.001505437781306804	0.0016804886861099208	0.0012253563336218172	50.66944367422047	1.0161985105268612	4.201221715274802E-4
240-241	0.001505437781306804	0.0016804886861099208	0.0012253563336218172	50.74864020457149	1.0173608485347538	4.201221715274802E-4
>>END_MODULE
