##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	SRR5665183
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2693087
Total Bases	669 Mbp
Sequences flagged as poor quality	0
Sequence length	75-252
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	28.007363668533547	32.0	25.0	33.0	18.0	33.0
2	28.29090742333983	30.0	27.0	31.0	18.0	33.0
3	30.869191006454674	33.0	30.0	33.0	27.0	33.0
4	30.77643574084313	33.0	31.0	33.0	28.0	33.0
5	31.82396818223845	33.0	31.0	33.0	29.0	34.0
6	35.71305568665253	37.0	36.0	38.0	31.0	38.0
7	36.01822295380728	38.0	36.0	38.0	33.0	38.0
8	36.505083942702186	38.0	37.0	38.0	34.0	38.0
9	36.66697993789283	38.0	38.0	38.0	34.0	38.0
10-14	36.813353671827166	38.0	38.0	38.0	34.8	38.0
15-19	36.84962208796077	38.0	38.0	38.0	35.0	38.0
20-24	36.8619511363725	38.0	38.0	38.0	35.0	38.0
25-29	36.81724051246766	38.0	38.0	38.0	35.0	38.0
30-34	36.80014481522505	38.0	38.0	38.0	35.0	38.0
35-39	36.78250572669951	38.0	38.0	38.0	35.0	38.0
40-44	36.76707362220381	38.0	38.0	38.0	35.0	38.0
45-49	36.7528968800488	38.0	38.0	38.0	34.8	38.0
50-54	36.71391165602894	38.0	38.0	38.0	34.0	38.0
55-59	36.65495522424637	38.0	38.0	38.0	34.0	38.0
60-64	36.623025843576535	38.0	38.0	38.0	34.0	38.0
65-69	36.57224790732717	38.0	38.0	38.0	34.0	38.0
70-74	36.51786006170614	38.0	38.0	38.0	34.0	38.0
75-79	36.43621748989837	38.0	38.0	38.0	34.0	38.0
80-84	36.34591830256809	38.0	38.0	38.0	33.8	38.0
85-89	36.235727372775884	38.0	37.0	38.0	33.0	38.0
90-94	36.150451908046215	38.0	37.0	38.0	33.0	38.0
95-99	36.0572076617862	38.0	37.0	38.0	33.0	38.0
100-104	35.95116754919309	38.0	37.0	38.0	31.4	38.0
105-109	35.8182762898253	38.0	36.8	38.0	31.0	38.0
110-114	35.68283063301347	38.0	36.0	38.0	31.0	38.0
115-119	35.518533445325396	38.0	36.0	38.0	30.8	38.0
120-124	35.48952482376363	38.0	35.4	38.0	31.0	38.0
125-129	32.76276092364009	34.4	32.8	35.4	27.6	35.6
130-134	33.44365143535235	36.0	33.8	36.4	25.6	37.2
135-139	34.65452179332361	38.0	35.0	38.0	26.0	38.0
140-144	34.55533579234319	38.0	35.0	38.0	25.8	38.0
145-149	34.51443803952749	38.0	35.0	38.0	25.0	38.0
150-154	34.46585881395685	38.0	35.0	38.0	25.0	38.0
155-159	34.44316802571978	38.0	35.0	38.0	25.0	38.0
160-164	34.4170594183595	38.0	35.0	38.0	24.8	38.0
165-169	34.33284071426458	38.0	34.0	38.0	24.0	38.0
170-174	34.27643209016882	38.0	34.0	38.0	24.0	38.0
175-179	34.206932319837435	38.0	34.0	38.0	20.4	38.0
180-184	34.130042253480184	38.0	34.0	38.0	16.0	38.0
185-189	34.03059396309486	38.0	34.0	38.0	16.0	38.0
190-194	33.91947147202428	38.0	34.0	38.0	16.0	38.0
195-199	33.828447956078676	38.0	34.0	38.0	16.0	38.0
200-204	33.68313653089729	38.0	34.0	38.0	15.8	38.0
205-209	33.57140031410336	38.0	33.2	38.0	15.0	38.0
210-214	33.50315636695679	38.0	33.0	38.0	15.0	38.0
215-219	33.3315151739852	38.0	33.0	38.0	15.0	38.0
220-224	33.219537851447406	38.0	33.0	38.0	15.0	38.0
225-229	33.07886827447698	38.0	32.0	38.0	15.0	38.0
230-234	32.93026398395951	37.6	31.0	38.0	15.0	38.0
235-239	32.80877310925457	37.0	31.0	38.0	15.0	38.0
240-244	32.79892910829128	37.0	31.0	38.0	15.0	38.0
245-249	33.281883355939335	37.6	31.6	38.0	20.0	38.0
250-252	35.19527446015877	37.333333333333336	33.333333333333336	38.0	30.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
13	1.0
14	0.0
15	0.0
16	2.0
17	11.0
18	17.0
19	62.0
20	356.0
21	1591.0
22	5004.0
23	10627.0
24	18781.0
25	28723.0
26	39568.0
27	50782.0
28	63546.0
29	75775.0
30	87991.0
31	103277.0
32	120726.0
33	145279.0
34	184594.0
35	260924.0
36	467607.0
37	1027843.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	39.44010885348352	23.509365879425374	13.788776546130677	23.261748720960423
2	15.68860074190422	22.35860931947554	39.37235199976235	22.580437938857884
3	17.49563788929912	30.099399311642404	29.87378377966913	22.531179019389345
4	14.021596937617964	20.689459267073563	38.35244351514463	26.936500280163845
5	15.29489218434433	35.88838607551459	35.25126909113729	13.565452649003795
6	28.03733424629151	38.22192978514911	20.49833703232399	13.242398936235391
7	23.39545658940836	31.790989299640156	26.783353081426633	18.030201029524854
8	22.808703472521856	35.708402925120104	22.730837411059284	18.752056191298756
9	23.224720515483554	17.675949831475673	21.75424968335547	37.3450799696853
10-14	23.45082995781089	23.93848257483315	29.27516109342494	23.335526373931025
15-19	22.840490907347725	28.63977712413704	26.413224142142326	22.106507826372905
20-24	22.68041734760852	27.42533503230275	27.27018945865624	22.624058161432494
25-29	22.89067793217834	27.37381052233824	26.985011343070493	22.75050020241293
30-34	22.768236763894848	27.30452210044006	27.196939686562803	22.73030144910229
35-39	22.73751303466059	27.160693887684577	27.28118350150809	22.82060957614674
40-44	22.548961209228455	26.90136248540916	27.54463706123118	23.005039244131208
45-49	22.282322316151827	26.850932910990505	27.806551636423727	23.060193136433938
50-54	22.002242205417563	26.900145069026983	27.733523332615935	23.36408939293952
55-59	21.84533351156759	26.709153257323848	27.666167239429875	23.77934599167869
60-64	21.878174797912045	26.67345640211552	27.388703096166893	24.05966570380554
65-69	21.93029203514718	26.723253034399043	27.04235154990177	24.30410338055201
70-74	22.125706916952765	26.737674667926992	26.79825985788547	24.338358557234773
75-79	22.241371013163306	26.862452203112706	26.66905156003812	24.22712522368587
80-84	22.27593511655323	26.82527582980654	26.618837006088224	24.279952047552005
85-89	22.233015715003663	26.532890730813286	26.786527908890246	24.447565645292805
90-94	22.24404784981671	26.220840097520153	26.868241290631357	24.666870762031788
95-99	22.106664353413237	26.052301313340724	27.055595768469274	24.785438564776765
100-104	22.05709213189532	26.108381884550745	27.078373860268158	24.75615212328578
105-109	21.842351366726227	26.71276307100304	26.87084205226225	24.574043510008487
110-114	21.665622392021568	27.31994804749116	26.592501501415622	24.42192805907165
115-119	21.56855257337476	27.518043631017647	26.535463853524927	24.37793994208267
120-124	21.60841808352175	27.628536650297576	26.439420368614453	24.32362489756622
125-129	22.556342848616538	25.563271815053483	28.53237589876062	23.34800943756936
130-134	21.206570218647595	31.456952157705338	28.026510202428444	19.309967421218623
135-139	23.599552794886243	23.457053056996383	28.463211057363896	24.480183090753478
140-144	22.518714039198525	27.30010883331346	26.78782107097839	23.393356056509628
145-149	22.46506253069443	27.239172470771734	27.5176379930276	22.778127005506242
150-154	22.621450304487002	27.111994662622184	27.302678283992382	22.963876748898436
155-159	22.51323697484216	27.038984342504925	27.373004328106298	23.07477435454662
160-164	22.459433328764938	26.926531098436847	27.478385191586362	23.135650381211853
165-169	22.398740077456093	26.872204920498216	27.409592524160658	23.319462477885033
170-174	22.36815104399028	26.906414595881312	27.42922054833646	23.29621381179195
175-179	22.35907313372728	26.62822572033057	27.431527858351945	23.581173287590207
180-184	22.36705372794816	26.49728011832974	27.481854190368026	23.65381196335407
185-189	22.147115216100488	26.219679211447435	27.8781417536685	23.75506381878358
190-194	22.050142416157435	26.244537238486092	27.797261636520066	23.908058708836407
195-199	21.870027889419514	26.15450146354416	28.05885812756651	23.91661251946982
200-204	22.06487675319608	25.89042619398555	27.80430036064081	24.24039669217756
205-209	22.136143355641746	25.62127052377124	28.036556784773513	24.206029335813497
210-214	22.093824618746353	25.69128359282799	27.745988617708843	24.468903170716814
215-219	21.905353538028553	25.701716319723232	27.716222218744697	24.676707923503514
220-224	21.96755817972411	25.431652605058552	28.27907346478054	24.321715750436795
225-229	21.731146305048565	25.675286220865356	28.058707517914165	24.534859956171914
230-234	21.839565211239098	25.791858231006753	27.967532343689346	24.401044214064807
235-239	22.032597825449734	25.465114922992665	28.232476021330516	24.269811230227084
240-244	21.912958982186673	25.314167526007076	28.22867819233588	24.54419529947037
245-249	21.656216259495658	25.834184763842998	28.124591792204136	24.38500718445721
250-252	20.61368210082569	26.12509001706625	28.754369321526163	24.506858560581897
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.5
12	0.5
13	0.0
14	0.8333333333333333
15	0.8333333333333333
16	0.0
17	1.9999999999999998
18	5.833333333333332
19	13.500000000000007
20	19.000000000000004
21	43.000000000000014
22	78.5
23	128.99999999999932
24	242.00000000000233
25	431.8333333333267
26	664.3333333333375
27	1101.5000000000334
28	1790.6666666665458
29	2785.666666666797
30	4217.000000000687
31	6266.166666665511
32	9132.833333331866
33	12825.8333333385
34	17562.50000000836
35	23211.499999987973
36	29893.999999963973
37	37849.666666652935
38	46421.1666667149
39	55263.33333344525
40	64618.166666845835
41	73519.66666675208
42	80678.66666664886
43	85540.66666657942
44	87032.9999998917
45	85898.8333332418
46	81624.66666663755
47	74496.50000007328
48	66119.83333352185
49	57080.666666789475
50	48821.83333339655
51	41566.166666678226
52	35432.666666634424
53	30046.1666666308
54	25777.83333331262
55	22521.499999990818
56	19996.83333333313
57	17788.50000000776
58	16037.833333344133
59	14321.666666674439
60	12776.666666671717
61	11233.500000002305
62	9997.333333333409
63	8811.999999997932
64	7562.999999997689
65	6330.499999998796
66	5154.166666666527
67	4106.000000000716
68	3164.8333333336263
69	2358.3333333332635
70	1776.6666666665508
71	1237.0000000000039
72	852.1666666666916
73	580.9999999999949
74	375.83333333333
75	251.00000000000247
76	140.4999999999995
77	90.49999999999984
78	56.166666666666764
79	31.499999999999964
80	16.66666666666668
81	7.499999999999997
82	2.833333333333333
83	1.5
84	0.6666666666666666
85	0.6666666666666666
86	0.3333333333333333
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.03817180804036409
2	6.312458528075774E-4
3	3.713210898868102E-4
4	7.426421797736204E-4
5	6.683779617962584E-4
6	3.713210898868102E-4
7	0.0
8	3.713210898868102E-5
9	1.4852843595472407E-4
10-14	8.317592413464548E-4
15-19	1.4852843595472409E-5
20-24	1.2624917056151545E-4
25-29	0.002866598813926175
30-34	0.003037406515274107
35-39	0.0027774817523533403
40-44	0.011221323336379405
45-49	0.006386722746053136
50-54	8.985970375260807E-4
55-59	7.426421797736204E-5
60-64	8.169063977509824E-5
65-69	7.4264217977362045E-6
70-74	7.27789336178148E-4
75-79	5.792628360559924E-4
80-84	0.002101700156602654
85-89	0.0017081153227376216
90-94	0.004909039799984478
95-99	0.0021760547088375674
100-104	0.001522540619712653
105-109	8.392977944442349E-4
110-114	5.942487859683006E-4
115-119	0.0012481387502356975
120-124	7.952350703719865E-4
125-129	0.0012682738680748983
130-134	0.0027200554771754466
135-139	0.0022492766445873884
140-144	0.001105956622587819
145-149	0.001285300939764222
150-154	0.0016888259058122363
155-159	0.001703773304891279
160-164	0.0018457567584027703
165-169	0.0018233422026975152
170-174	0.0023613890766624964
175-179	0.0035495797073442524
180-184	0.004797648703756776
185-189	0.00349004090298045
190-194	0.0021824036530447557
195-199	0.0014128406282132313
200-204	0.0013013684337828961
205-209	0.0018418213846543635
210-214	0.004695270917131919
215-219	0.013705927956768102
220-224	0.016001784221606124
225-229	0.017255348587700288
230-234	0.03063629155878297
235-239	0.08524963181793725
240-244	0.036823936669590045
245-249	9.490687435511858E-5
250-252	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
75-79	19.0
80-84	33.0
85-89	32.0
90-94	33.0
95-99	67.0
100-104	105.0
105-109	195.0
110-114	358.0
115-119	675.0
120-124	2233.0
125-129	12921.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150-154	3.0
155-159	3.0
160-164	3.0
165-169	12.0
170-174	13.0
175-179	33.0
180-184	104.0
185-189	187.0
190-194	323.0
195-199	799.0
200-204	2341.0
205-209	3674.0
210-214	6590.0
215-219	9799.0
220-224	14526.0
225-229	22630.0
230-234	37650.0
235-239	56333.0
240-244	107931.0
245-249	356088.0
250-253	2057374.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	89.65109427963127
#Duplication Level	Percentage of total
1	82.50371536075004
2	11.371622330418846
3	2.7584063171387965
4	1.0644914053357803
5	0.4737505542196198
6	0.30608470126813475
7	0.21743437932099796
8	0.17961958703300918
9	0.12882693240931753
>10	0.9682739236202501
>50	0.027774508485237076
>100	0.0
>500	0.0
>1k	0.0
>5k	0.0
>10k+	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	0.0	0.0	0.0	0.0	0.0	0.0
2	0.0	3.713210898868102E-5	0.0	0.0	0.0	0.0
3	0.0	3.713210898868102E-5	0.0	0.0	0.0	0.0
4	0.0	3.713210898868102E-5	0.0	3.713210898868102E-5	0.0	0.0
5	0.0	3.713210898868102E-5	0.0	3.713210898868102E-5	0.0	0.0
6	0.0	7.426421797736204E-5	0.0	7.426421797736204E-5	0.0	0.0
7	0.0	7.426421797736204E-5	0.0	7.426421797736204E-5	3.713210898868102E-5	0.0
8	0.0	7.426421797736204E-5	3.713210898868102E-5	1.1139632696604306E-4	3.713210898868102E-5	0.0
9	0.0	7.426421797736204E-5	3.713210898868102E-5	1.1139632696604306E-4	3.713210898868102E-5	0.0
10-14	2.9705687190944815E-5	7.426421797736204E-5	3.713210898868102E-5	2.0051338853887749E-4	3.713210898868102E-5	0.0
15-19	1.6338127955019648E-4	7.426421797736204E-5	3.713210898868102E-5	4.158796206732274E-4	5.198495258415343E-5	0.0
20-24	2.0051338853887749E-4	7.426421797736204E-5	3.713210898868102E-5	0.001708077013479327	7.426421797736204E-5	0.0
25-29	2.2279265393208614E-4	9.654348337057065E-5	3.713210898868102E-5	0.03601071929722285	7.426421797736204E-5	0.0
30-34	2.4507191932529474E-4	2.3021907572982227E-4	5.941137438188963E-5	0.3333795009221759	2.3764549752755852E-4	1.4852843595472407E-5
35-39	2.599247629207671E-4	2.599247629207671E-4	2.079398103366137E-4	1.0201824151986176	3.861739334822826E-4	7.426421797736204E-5
40-44	2.599247629207671E-4	2.9705687190944815E-4	3.341889808981292E-4	2.3306636584707436	4.530117296619084E-4	1.0396990516830685E-4
45-49	2.599247629207671E-4	3.193361373026568E-4	3.341889808981292E-4	3.8446140061572462	5.569816348302153E-4	1.1139632696604307E-4
50-54	3.341889808981292E-4	3.341889808981292E-4	3.713210898868102E-4	5.4820063369657195	6.758043835939946E-4	1.1139632696604307E-4
55-59	4.307324642686998E-4	3.713210898868102E-4	3.713210898868102E-4	7.160125164912979	9.951405208966514E-4	1.1139632696604307E-4
60-64	5.198495258415342E-4	3.713210898868102E-4	3.713210898868102E-4	8.937913999807655	0.0015224164685359218	1.2624917056151545E-4
65-69	5.569816348302153E-4	3.713210898868102E-4	3.713210898868102E-4	10.764806335628965	0.001886311136624996	1.4852843595472407E-4
70-74	5.569816348302153E-4	3.861739334822826E-4	3.861739334822826E-4	12.683437259917708	0.0020645452597706643	1.4852843595472407E-4
75-79	5.569816348302153E-4	4.2330604247096357E-4	4.307324642686998E-4	14.645208268429503	0.0022279265393208613	1.559548577524603E-4
80-84	5.866873220211601E-4	4.7529099505511704E-4	4.8271741685285325E-4	16.704911501188043	0.0025398362548257813	1.856605449434051E-4
85-89	5.941137438188963E-4	4.901438386505895E-4	5.421287912347429E-4	18.816347188189614	0.0028963045011171193	1.856605449434051E-4
90-94	5.941137438188963E-4	5.198495258415342E-4	6.386722746053135E-4	20.999871151581807	0.005280185898190441	1.856605449434051E-4
95-99	6.089665874143687E-4	5.198495258415342E-4	6.683779617962584E-4	23.223052207373918	0.03683505211677157	1.856605449434051E-4
100-104	6.312458528075773E-4	5.421287912347429E-4	6.683779617962584E-4	25.499101959944106	0.10706672305796286	1.856605449434051E-4
105-109	6.312458528075773E-4	5.644080566279515E-4	6.980836489872031E-4	27.80801362896928	0.23495713283677802	1.856605449434051E-4
110-114	6.460986964030497E-4	5.941137438188963E-4	7.055100707849393E-4	30.118054114107714	0.34800955186371624	1.856605449434051E-4
115-119	6.832308053917308E-4	6.163930092121048E-4	7.055100707849393E-4	31.500460252490917	0.43180186900757384	1.856605449434051E-4
120-124	7.055100707849393E-4	6.312458528075773E-4	7.055100707849393E-4	31.53184431100815	0.44248848997451623	1.856605449434051E-4
125-129	7.352157579758842E-4	6.312458528075773E-4	7.426421797736204E-4	31.53217107356725	0.4425107692399094	1.856605449434051E-4
130-134	7.64921445166829E-4	6.312458528075773E-4	7.574950233690929E-4	31.5326389381405	0.44254047492710036	1.856605449434051E-4
135-139	7.872007105600376E-4	6.53525118200786E-4	8.243328195487187E-4	31.53356724086522	0.44254047492710036	2.079398103366137E-4
140-144	8.39185663144191E-4	7.129364925826756E-4	8.540385067396635E-4	31.534673777713085	0.4426147391450777	2.747776065162396E-4
145-149	8.911706157283446E-4	8.020535541555099E-4	8.763177721328721E-4	31.536240752712406	0.44272613547204376	2.9705687190944815E-4
150-154	9.060234593238169E-4	8.540385067396635E-4	9.208763029192894E-4	31.538565222735098	0.44280039969002116	2.9705687190944815E-4
155-159	0.0010025669426943875	8.985970375260808E-4	0.00101741978628986	31.54141696870543	0.44280039969002116	2.9705687190944815E-4
160-164	0.0010025669426943875	9.283027247170255E-4	0.0010768311606717497	31.545501500694183	0.44280039969002116	2.9705687190944815E-4
165-169	0.0010322726298853321	9.283027247170255E-4	0.0011733746440423204	31.550291542753722	0.44282267895541433	3.119097155049206E-4
170-174	0.0010768311606717497	9.283027247170255E-4	0.0012550652838174182	31.555460332324948	0.4429117960169872	3.341889808981292E-4
175-179	0.0010768311606717497	9.431555683124977E-4	0.0012624917056151545	31.561639115260665	0.4429934866567623	3.5646824629133783E-4
180-184	0.0010842575824694858	9.654348337057065E-4	0.0012624917056151545	31.56999383978312	0.44306032445294197	4.307324642686998E-4
185-189	0.0011139632696604306	9.654348337057065E-4	0.0012624917056151545	31.57897981015838	0.4431642943581102	4.7529099505511704E-4
190-194	0.0011362425350536392	9.654348337057065E-4	0.0012847709710083633	31.588463350794086	0.4433276756376604	5.198495258415342E-4
195-199	0.0011659482222445843	9.728612555034427E-4	0.0013367559235925167	31.59932077946238	0.44381781947631105	5.198495258415342E-4
200-204	0.0012179331748287375	0.0010619783170762772	0.001425872985165351	31.60923505256236	0.44677353535181	5.198495258415342E-4
205-209	0.0012624917056151545	0.0010768311606717497	0.0014778579377495045	31.619446382534242	0.45401429660460285	5.198495258415342E-4
210-214	0.0012699181274128907	0.0010768311606717497	0.0015521221557268667	31.629271538572652	0.4639285697045807	5.198495258415342E-4
215-219	0.0012996238146038357	0.0010768311606717497	0.0015818278429178111	31.64501555278385	0.47521673083713967	5.198495258415342E-4
220-224	0.0012996238146038357	0.0010768311606717497	0.0015966806865132838	31.662482496852125	0.4897873704042981	5.198495258415342E-4
225-229	0.0013293295017947806	0.0010768311606717497	0.0015966806865132838	31.67869437563659	0.5048852859190958	5.347023694370066E-4
230-234	0.0013441823453902528	0.0010768311606717497	0.0016635184826929098	31.694111627288684	0.5177775541599658	5.569816348302153E-4
235-239	0.0013738880325811977	0.0010768311606717497	0.0016709449044906457	31.712157832257184	0.5255678706257912	5.569816348302153E-4
240-241	0.0013738880325811977	0.0011139632696604306	0.001670944904490646	31.762508972045836	0.5259020596066892	5.569816348302153E-4
>>END_MODULE
