##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	SRR5665186
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5453966
Total Bases	1.3 Gbp
Sequences flagged as poor quality	0
Sequence length	75-252
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	28.688097615570026	32.0	25.0	33.0	18.0	33.0
2	30.230490802472914	31.0	29.0	33.0	25.0	33.0
3	31.332364008136466	33.0	31.0	33.0	28.0	34.0
4	30.75424489261576	33.0	31.0	33.0	27.0	33.0
5	31.420623817603555	33.0	31.0	33.0	29.0	34.0
6	34.70855447210342	37.0	34.0	38.0	28.0	38.0
7	35.922583492453015	38.0	36.0	38.0	31.0	38.0
8	35.80239444103612	38.0	36.0	38.0	31.0	38.0
9	36.38798903403505	38.0	37.0	38.0	33.0	38.0
10-14	36.67917079057698	38.0	37.8	38.0	34.0	38.0
15-19	36.70220357075934	38.0	38.0	38.0	34.0	38.0
20-24	36.72223875249681	38.0	38.0	38.0	34.0	38.0
25-29	36.666134699042864	38.0	38.0	38.0	34.0	38.0
30-34	36.63771295970675	38.0	38.0	38.0	34.0	38.0
35-39	36.6025934888483	38.0	38.0	38.0	34.0	38.0
40-44	36.562830167991514	38.0	38.0	38.0	34.0	38.0
45-49	36.53669777185996	38.0	38.0	38.0	34.0	38.0
50-54	36.477282439971205	38.0	38.0	38.0	34.0	38.0
55-59	36.38606371950247	38.0	37.2	38.0	33.6	38.0
60-64	36.32645828741873	38.0	37.0	38.0	33.0	38.0
65-69	36.2488575836373	38.0	37.0	38.0	33.0	38.0
70-74	36.15725030189041	38.0	37.0	38.0	33.0	38.0
75-79	36.03521877052674	38.0	37.0	38.0	32.2	38.0
80-84	35.89320490822398	38.0	37.0	38.0	31.0	38.0
85-89	35.73312514161764	38.0	36.4	38.0	31.0	38.0
90-94	35.611150843825975	38.0	36.0	38.0	29.4	38.0
95-99	35.46647579100291	38.0	36.0	38.0	29.0	38.0
100-104	35.31029653281246	38.0	36.0	38.0	28.4	38.0
105-109	35.10301455781752	38.0	35.0	38.0	27.8	38.0
110-114	34.91517093808479	38.0	35.0	38.0	27.0	38.0
115-119	34.74359172587059	38.0	34.2	38.0	26.6	38.0
120-124	34.84407926080748	38.0	34.0	38.0	28.0	38.0
125-129	33.75528584714754	34.2	33.2	35.6	31.0	36.2
130-134	35.400005969204436	36.2	36.0	37.2	33.0	38.0
135-139	36.583029146482986	38.0	38.0	38.0	34.0	38.0
140-144	36.53253159302775	38.0	38.0	38.0	34.0	38.0
145-149	36.51130628116906	38.0	38.0	38.0	34.0	38.0
150-154	36.49022198155039	38.0	38.0	38.0	34.0	38.0
155-159	36.47806532601916	38.0	38.0	38.0	34.0	38.0
160-164	36.45505216642097	38.0	38.0	38.0	34.0	38.0
165-169	36.39782127538317	38.0	38.0	38.0	34.0	38.0
170-174	36.35278215597138	38.0	38.0	38.0	33.4	38.0
175-179	36.28616043385012	38.0	38.0	38.0	33.0	38.0
180-184	36.19845894299853	38.0	37.0	38.0	33.0	38.0
185-189	36.08159689126438	38.0	37.0	38.0	32.8	38.0
190-194	35.963702791529066	38.0	37.0	38.0	31.2	38.0
195-199	35.82844487347482	38.0	37.0	38.0	31.0	38.0
200-204	35.65099891764995	38.0	37.0	38.0	29.4	38.0
205-209	35.47785489840584	38.0	36.2	38.0	29.0	38.0
210-214	35.29258242288185	38.0	36.0	38.0	28.0	38.0
215-219	35.022701052351664	38.0	35.4	38.0	26.4	38.0
220-224	34.83076906531477	38.0	35.0	38.0	25.4	38.0
225-229	34.61229031820195	38.0	35.0	38.0	24.0	38.0
230-234	34.37051045967897	38.0	34.0	38.0	23.0	38.0
235-239	34.147680226784175	38.0	34.0	38.0	23.0	38.0
240-244	34.003665856638904	38.0	33.4	38.0	23.0	38.0
245-249	34.26518601667811	38.0	33.2	38.0	24.0	38.0
250-252	35.59137212361105	37.666666666666664	34.333333333333336	38.0	31.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
16	9.0
17	35.0
18	49.0
19	96.0
20	329.0
21	1118.0
22	3385.0
23	7421.0
24	14196.0
25	23359.0
26	35697.0
27	50127.0
28	68413.0
29	90326.0
30	117369.0
31	151127.0
32	196555.0
33	263540.0
34	374469.0
35	591173.0
36	1135556.0
37	2329617.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.98638602329695	19.76576124477352	11.91795223956373	22.329900492365798
2	16.75557150319612	20.695626791473863	40.20021052596838	22.348591179361634
3	19.011458294561763	28.980081053137052	29.490884868278112	22.517575784023077
4	14.088954790290101	20.61482535833276	37.235326614397415	28.060893236979734
5	16.85595194810648	34.68593421596179	33.817680695614925	14.640433140316803
6	28.569610753602575	37.41065418836883	19.853100936587413	14.166634121441179
7	24.31245445974544	30.661283917061454	26.13571481743744	18.890546805755665
8	23.850043042080397	34.01899352122722	22.28582324969082	19.84514018700157
9	23.388190090066637	16.956847150750225	21.61063828460851	38.04432447457463
10-14	23.675517118784818	23.47591026712708	29.087861742038434	23.760710872049668
15-19	23.03124001873132	28.42445662477544	26.21885064923397	22.32545270725927
20-24	22.8682533126653	27.25870943943905	27.06290682123956	22.810130426656087
25-29	23.09083822155363	27.155588809278715	26.803619345789837	22.949953623377823
30-34	22.935631077858936	26.964123442465564	27.061431527159353	23.03881395251615
35-39	22.73686620047451	26.649574738917025	27.424025211326864	23.189533849281602
40-44	22.412631338824873	26.233301799240362	27.823085853356016	23.530981008578753
45-49	21.750927757834496	26.114430672460827	28.379137852383955	23.755503717320725
50-54	21.255009523071912	26.248921783195488	28.198117006793144	24.297951686939452
55-59	20.979654200693616	25.964508145015774	27.926839670219877	25.128997984070732
60-64	21.067374321392062	25.899852716169935	27.211952740680566	25.820820221757433
65-69	20.885137229321728	25.850493079750997	26.70076613611204	26.563603554815234
70-74	20.613021250445502	25.715176607146283	26.450750475080433	27.221051667327785
75-79	20.126631283181183	25.44006549170651	26.699429144168775	27.733874080943526
80-84	19.78270883549446	25.209285374357833	26.89273282701079	28.11527296313691
85-89	19.744573674938394	24.666947240752148	27.25155752723477	28.33692155707469
90-94	19.776402707502434	24.04117968638197	27.55023240894703	28.632185197168564
95-99	19.59003988686911	23.74349540653315	28.024420493186486	28.642044213411253
100-104	19.464256064816865	24.121446089699678	28.062465278291004	28.351832567192453
105-109	19.15201843728109	25.45347320630853	27.662824759485577	27.7316835969248
110-114	18.852276498225994	26.7614523742392	27.196459138410596	27.18981198912421
115-119	18.808409697570102	27.208475742315418	27.05826063824908	26.9248539218654
120-124	19.000812417751188	27.43474736597355	26.90008582963231	26.664354386642948
125-129	22.43986100247636	25.295264476737245	27.8563419902044	24.408532530582004
130-134	22.197672468661633	30.141817877520964	27.093219719749833	20.567289934067563
135-139	23.829664946664405	23.201474026560717	28.16328431653603	24.805576710238846
140-144	22.637150809643128	27.202006824571317	26.69998010980068	23.46086225598488
145-149	22.589194724076787	26.98436403615193	27.397346739325446	23.029094500445837
150-154	22.62498406803622	26.791053957761417	27.36381980083967	23.220142173362685
155-159	22.464593289411443	26.60878074778743	27.471932970315322	23.45469299248581
160-164	22.28263761323541	26.53385237762484	27.6539140049839	23.529596004155852
165-169	22.20542691744953	26.355156875258235	27.504290301278957	23.935125906013283
170-174	22.09632480675845	26.400594058763826	27.52065954908487	23.982421585392853
175-179	22.122017799631745	25.953212815584507	27.458904077534896	24.465865307248844
180-184	22.161114069661085	25.649511658683004	27.422261043900985	24.767113227754926
185-189	21.744330412411102	25.02022193937796	28.291001778734614	24.94444586947632
190-194	21.623516245236395	25.327590190995387	27.81142700581019	25.237466557958026
195-199	21.404992053710377	25.105234920402342	28.27667150551576	25.213101520371524
200-204	21.84704463347536	24.7734627837685	27.69959316585937	25.679899416896767
205-209	22.082885129924907	24.26681321900842	27.93315434376465	25.717147307302024
210-214	22.14757407126898	24.48776626580996	27.210437719351475	26.154221943569585
215-219	21.701325355109642	24.651843826702752	27.27019501819005	26.376635799997555
220-224	21.876625085688463	24.62555592204156	27.85253759528688	25.645281396983094
225-229	21.566453595951216	25.194503448674894	27.4652816250805	25.773761330293393
230-234	21.881525532773345	25.290848198882316	27.413260884065345	25.414365384278998
235-239	22.221344241270636	25.00337876044417	27.34098860068661	25.434288397598586
240-244	22.024390670427724	24.859071447674946	27.514845349234463	25.601692532662867
245-249	21.722648632677853	25.70655811255923	27.2596741188186	25.31111913594432
250-252	20.722735526316345	26.243958749092837	27.50145922919921	25.53184649539161
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.6666666666666666
9	0.6666666666666666
10	1.3333333333333333
11	1.9999999999999998
12	4.0
13	7.9999999999999964
14	17.33333333333334
15	21.333333333333325
16	39.5
17	78.16666666666667
18	153.49999999999991
19	273.66666666666873
20	446.8333333333258
21	752.5000000000143
22	1160.5000000000184
23	1720.9999999998918
24	2519.3333333333485
25	3681.3333333338733
26	5089.833333333216
27	6880.166666664935
28	9312.999999998918
29	12454.333333337921
30	15966.333333344275
31	20271.66666666501
32	25082.1666666477
33	30707.833333294046
34	37944.83333332122
35	46607.00000005025
36	56382.500000120905
37	67606.33333350059
38	80482.99999998126
39	94433.66666644659
40	108574.66666624277
41	122104.99999938106
42	133684.16666595166
43	142815.6666662158
44	148305.8333330396
45	149434.49999973754
46	146561.66666631933
47	139097.16666610038
48	128509.33333262822
49	115983.66666614225
50	103619.33333298822
51	91709.83333316083
52	80595.33333331966
53	71231.33333345372
54	63580.833333503535
55	56971.000000122665
56	51188.16666674799
57	46679.66666671578
58	42672.50000002037
59	39245.666666662524
60	36065.6666666398
61	33028.49999995291
62	29660.66666663177
63	26147.666666644378
64	22610.666666657056
65	19141.000000002918
66	15631.33333334341
67	12369.83333333756
68	9492.16666666572
69	7029.499999998204
70	5073.166666666618
71	3583.000000000473
72	2408.8333333332844
73	1596.8333333332605
74	1024.666666666709
75	618.3333333333316
76	395.8333333333291
77	232.66666666666853
78	130.49999999999937
79	75.66666666666673
80	39.499999999999986
81	19.666666666666668
82	7.499999999999997
83	1.8333333333333333
84	0.3333333333333333
85	0.0
86	0.0
87	0.5
88	0.5
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.03524041037292862
2	1.2834696805957352E-4
3	2.3835865496777943E-4
4	4.950525910869264E-4
5	5.317231533896618E-4
6	3.1169977957325E-4
7	0.0
8	1.833528115136765E-5
9	7.33411246054706E-5
10-14	8.250876518115441E-4
15-19	0.0
20-24	7.700818083574412E-5
25-29	0.002577940529882291
30-34	0.0028236332973106174
35-39	0.0029959849401334734
40-44	0.011166186221182896
45-49	0.006120316848326521
50-54	9.827710697133058E-4
55-59	8.80093495265647E-5
60-64	1.246799118293E-4
65-69	7.334112460547059E-6
70-74	8.690923265748263E-4
75-79	8.837664497906245E-4
80-84	0.0026256820676732883
85-89	0.0020059813449602505
90-94	0.0055633631303933125
95-99	0.002596562034507576
100-104	0.0018081460905754292
105-109	0.0012250876231084684
110-114	5.869413043288462E-4
115-119	0.001313539347181218
120-124	0.0010239992389373398
125-129	0.0015016029611610395
130-134	0.0027856287350131975
135-139	0.0021039603276356298
140-144	0.0011090929222737733
145-149	0.0014222919202580612
150-154	0.0016728512419243934
155-159	0.0017907631112231916
160-164	0.002118708784435604
165-169	0.001916057731851184
170-174	0.002630922477326717
175-179	0.0037069369355327187
180-184	0.004554620568600159
185-189	0.003254126374126725
190-194	0.0022926631719157164
195-199	0.0011872374919276146
200-204	0.0013355217689863881
205-209	0.0019317173735705752
210-214	0.004365224849066519
215-219	0.012581243482056798
220-224	0.013629674372816928
225-229	0.01624332562279504
230-234	0.0302579151946695
235-239	0.0778704096103988
240-244	0.031822078678979085
245-249	1.3569245017274744E-4
250-252	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
75-79	103.0
80-84	117.0
85-89	148.0
90-94	155.0
95-99	217.0
100-104	364.0
105-109	543.0
110-114	904.0
115-119	1311.0
120-124	3384.0
125-129	18861.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150-154	1.0
155-159	15.0
160-164	19.0
165-169	37.0
170-174	77.0
175-179	145.0
180-184	346.0
185-189	750.0
190-194	1295.0
195-199	2332.0
200-204	4901.0
205-209	9043.0
210-214	19902.0
215-219	29743.0
220-224	44795.0
225-229	68970.0
230-234	110636.0
235-239	143477.0
240-244	236022.0
245-249	700154.0
250-253	4055199.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	91.65724688130759
#Duplication Level	Percentage of total
1	84.81949255608758
2	11.68678800370242
3	2.1835038656019448
4	0.6504161593148176
5	0.26607564799295735
6	0.1410468137501447
7	0.07591141418542162
8	0.044684460758024847
9	0.034354009273066435
>10	0.095974534051624
>50	0.0017525352820046416
>100	0.0
>500	0.0
>1k	0.0
>5k	0.0
>10k+	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	0.0	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	1.8335281151367646E-5	0.0	0.0
4	0.0	0.0	0.0	9.167640575683824E-5	0.0	0.0
5	0.0	0.0	0.0	1.1001168690820588E-4	0.0	0.0
6	0.0	0.0	1.8335281151367646E-5	1.2834696805957352E-4	0.0	0.0
7	0.0	0.0	3.667056230273529E-5	1.4668224921094117E-4	3.667056230273529E-5	0.0
8	0.0	0.0	3.667056230273529E-5	1.4668224921094117E-4	3.667056230273529E-5	0.0
9	1.8335281151367646E-5	0.0	3.667056230273529E-5	2.0168809266504412E-4	3.667056230273529E-5	0.0
10-14	5.500584345410294E-5	0.0	8.434229329629117E-5	3.8504090417872057E-4	3.667056230273529E-5	0.0
15-19	8.434229329629117E-5	7.3341124605470584E-6	1.1001168690820588E-4	9.937722384041263E-4	3.667056230273529E-5	0.0
20-24	1.2834696805957352E-4	1.8335281151367646E-5	1.2834696805957352E-4	0.005636265425930415	3.667056230273529E-5	3.6670562302735292E-6
25-29	1.613504741320353E-4	1.8335281151367646E-5	1.4668224921094117E-4	0.0940636593627463	3.667056230273529E-5	1.8335281151367646E-5
30-34	2.493598236586E-4	4.033761853300883E-5	1.8701986774395E-4	0.7930339133027232	5.280560971593882E-4	1.8335281151367646E-5
35-39	2.823633297310618E-4	5.500584345410294E-5	2.0168809266504412E-4	2.332948170193947	6.674042339097824E-4	1.8335281151367646E-5
40-44	3.3370211695489115E-4	8.067523706601765E-5	2.0168809266504412E-4	5.138711902494441	7.224100773638852E-4	1.8335281151367646E-5
45-49	3.887079604089941E-4	9.90105182173853E-5	2.3469159873750587E-4	8.321019969688113	8.470899891931854E-4	1.8335281151367646E-5
50-54	4.180444102511824E-4	1.6501753036230884E-4	2.6769510480996765E-4	11.601322780523384	0.0012211297246810852	1.8335281151367646E-5
55-59	5.280560971593882E-4	1.7968575528340294E-4	2.750292172705147E-4	14.886807141812032	0.0019105362959725089	1.8335281151367646E-5
60-64	5.390572658502088E-4	1.980210364347706E-4	2.9336449842188233E-4	18.15768195107927	0.0026109440359547527	2.5669393611914703E-5
65-69	5.683937156923971E-4	2.0168809266504412E-4	3.006986108824294E-4	21.367093230870896	0.0032050071452590645	3.667056230273529E-5
70-74	5.830619406134911E-4	2.273574862769588E-4	3.3003506072461767E-4	24.541880165736274	0.0037623996922606416	4.400467476328235E-5
75-79	6.233995591465001E-4	2.8969744219160874E-4	3.3003506072461767E-4	27.63843779004123	0.004118104146597174	5.500584345410294E-5
80-84	6.564030652189618E-4	2.9336449842188233E-4	3.3003506072461767E-4	30.690117980200096	0.004569152062920818	7.700818083574412E-5
85-89	6.8573951506115E-4	3.080327233429765E-4	3.3003506072461767E-4	33.64582397470024	0.005210886903218685	9.167640575683824E-5
90-94	7.150759649033382E-4	3.227009482640706E-4	3.520373981062588E-4	36.523825047680894	0.010183415151469592	1.1001168690820588E-4
95-99	7.187430211336118E-4	3.337021169548912E-4	3.8504090417872057E-4	39.30784313653588	0.066469061229938	1.1001168690820588E-4
100-104	7.737488645877148E-4	3.520373981062588E-4	3.887079604089941E-4	42.010848619151645	0.19294583061207202	1.1001168690820588E-4
105-109	8.214205955812707E-4	3.6670562302735295E-4	4.29045578942003E-4	44.61493525995578	0.40598712936604303	1.1001168690820588E-4
110-114	8.324217642720911E-4	3.8504090417872057E-4	4.6204908501446473E-4	47.10589321605598	0.5995343571998799	1.1001168690820588E-4
115-119	8.654252703445529E-4	4.070432415603617E-4	4.7671730993555886E-4	48.51387412389444	0.7379510616677845	1.1001168690820588E-4
120-124	9.094299451078353E-4	4.29045578942003E-4	4.7671730993555886E-4	48.545641832017296	0.7567153883980942	1.1001168690820588E-4
125-129	9.681028447922118E-4	4.547149725539176E-4	4.91385534856653E-4	48.54627989980136	0.7568180659725418	1.1734579936875294E-4
130-134	9.937722384041263E-4	4.91385534856653E-4	5.023867035474736E-4	48.54765504588771	0.7568510694786142	1.2834696805957352E-4
135-139	0.001037776913167409	5.06053759777747E-4	5.280560971593882E-4	48.54997262542524	0.7569060753220683	1.2834696805957352E-4
140-144	0.001081781587930691	5.133878722382941E-4	5.353902096199353E-4	48.553298645426096	0.7569610811655225	1.2834696805957352E-4
145-149	0.001081781587930691	5.280560971593882E-4	5.610596032318501E-4	48.5585315346667	0.7570820940211215	1.393481367503941E-4
150-154	0.0011441215438453413	5.463913783107559E-4	5.830619406134911E-4	48.566756741791195	0.7571811045393388	1.7968575528340294E-4
155-159	0.0011551227125361618	6.233995591464999E-4	5.867289968437647E-4	48.57858299813383	0.7572287762703326	2.0168809266504412E-4
160-164	0.0011661238812269823	6.417348402978677E-4	5.867289968437647E-4	48.59221711319799	0.7572764480013259	2.2002337381641176E-4
165-169	0.0011954603310691705	6.564030652189618E-4	5.940631093043117E-4	48.607274046079496	0.7573607902946222	2.2002337381641176E-4
170-174	0.001246799118293	6.8573951506115E-4	6.233995591465E-4	48.62287003622684	0.7574487996441489	2.273574862769588E-4
175-179	0.001246799118293	7.040747962125176E-4	6.307336716070471E-4	48.64100362928555	0.7575808136684387	2.4202571119805294E-4
180-184	0.0012651343994443676	7.40745358515253E-4	6.417348402978677E-4	48.665506899016236	0.7577018265240377	2.6769510480996765E-4
185-189	0.0012908037930562824	7.590806396666205E-4	6.417348402978677E-4	48.69239742235284	0.7579915239662294	2.750292172705147E-4
190-194	0.0013128061304379236	7.810829770482617E-4	6.417348402978677E-4	48.71838218280055	0.7585855870755336	2.750292172705147E-4
195-199	0.0013201402428984707	8.067523706601765E-4	6.527360089886882E-4	48.744410947923036	0.760400779909519	2.750292172705147E-4
200-204	0.0013201402428984707	8.287547080418177E-4	6.747383463703295E-4	48.77232457994788	0.7686773258212465	2.9703155465215587E-4
205-209	0.0013201402428984707	8.617582141142794E-4	6.784054026006029E-4	48.798841063548984	0.7885050988583354	3.3003506072461767E-4
210-214	0.0013311414115892914	8.837605514959206E-4	6.967406837519706E-4	48.82475614992833	0.8142258312574739	3.3003506072461767E-4
215-219	0.0013568108052012058	8.984287764170148E-4	6.967406837519706E-4	48.85874976118297	0.8468332952570661	3.3003506072461767E-4
220-224	0.0013568108052012058	9.130970013381089E-4	7.040747962125176E-4	48.89595938075155	0.8908269688516578	3.447032856457118E-4
225-229	0.0013824801988131206	9.461005074105705E-4	7.334112460547059E-4	48.92949827703363	0.9356677324354423	3.8504090417872057E-4
230-234	0.0014154837048855823	9.644357885619382E-4	7.40745358515253E-4	48.96093961715199	0.9710731603387333	3.8504090417872057E-4
235-239	0.0014374860422672238	9.82771069713306E-4	7.517465272060736E-4	49.00139091442814	0.9946009931121683	3.8504090417872057E-4
240-241	0.001448487210958044	9.901051821738528E-4	7.517465272060736E-4	49.08704968091111	0.9957432811278986	3.8504090417872057E-4
>>END_MODULE
