##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	SRR6235471
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	266664
Total Bases	31 Mbp
Sequences flagged as poor quality	0
Sequence length	30-183
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	34.23149731497315	34.0	31.0	36.0	31.0	41.0
2	34.542765427654274	34.0	31.0	41.0	31.0	41.0
3	34.65850283502835	34.0	31.0	41.0	31.0	41.0
4	37.331379563795636	37.0	37.0	41.0	35.0	41.0
5	37.22037095370954	37.0	37.0	41.0	35.0	41.0
6	37.216872168721686	37.0	37.0	41.0	35.0	41.0
7	37.3130456304563	37.0	37.0	41.0	35.0	41.0
8	37.317530675306756	37.0	37.0	41.0	35.0	41.0
9	38.51370638706387	39.0	38.0	41.0	35.0	41.0
10-14	38.73371133711338	39.4	38.4	41.0	35.2	41.0
15-19	39.43460759607596	41.0	40.0	41.0	36.0	41.0
20-24	39.509922599225995	41.0	40.0	41.0	36.8	41.0
25-29	39.49734047340473	41.0	40.0	41.0	36.8	41.0
30-34	39.448300148344686	41.0	40.0	41.0	36.2	41.0
35-39	39.50074056270901	41.0	40.2	41.0	36.6	41.0
40-44	39.621489879740494	41.0	41.0	41.0	37.0	41.0
45-49	39.67262141319783	41.0	41.0	41.0	37.0	41.0
50-54	39.67899198483592	41.0	41.0	41.0	37.0	41.0
55-59	39.71211218808118	41.0	41.0	41.0	37.0	41.0
60-64	39.75557482392455	41.0	41.0	41.0	36.4	41.0
65-69	39.80144422539273	41.0	41.0	41.0	36.2	41.0
70-74	39.918358163258944	41.0	41.0	41.0	37.2	41.0
75-79	40.02969581108506	41.0	41.0	41.0	40.6	41.0
80-84	40.255228299167264	41.0	41.0	41.0	41.0	41.0
85-89	40.37342143357605	41.0	41.0	41.0	41.0	41.0
90-94	40.36508475077774	41.0	41.0	41.0	41.0	41.0
95-99	39.99133294903244	41.0	41.0	41.0	40.6	41.0
100-104	36.52304208009752	38.6	35.4	40.2	31.8	41.0
105-109	35.86866546847489	38.0	34.0	40.0	30.0	41.0
110-114	35.942620170194935	38.0	34.0	40.0	30.0	41.0
115-119	36.027004609039395	38.0	34.0	40.0	30.0	41.0
120-124	36.12506901266768	38.0	34.0	40.0	30.0	41.0
125-129	36.18393868435625	38.0	34.6	40.0	30.0	41.0
130-134	36.28534486041258	38.0	35.0	40.0	30.6	41.0
135-139	36.31343931517266	38.0	34.8	40.0	31.0	41.0
140-144	36.31197349407957	38.0	35.0	40.0	31.0	41.0
145-149	36.23751190764258	37.4	34.2	40.0	31.0	41.0
150-154	36.12703013373308	37.0	34.0	39.8	31.0	41.0
155-159	35.94395775718361	37.0	34.0	39.0	31.0	41.0
160-164	35.57465693851397	37.0	34.0	39.0	31.0	40.4
165-169	34.94837911679688	36.6	33.0	38.0	30.6	39.2
170-174	34.07465089057791	34.8	32.0	37.0	30.0	38.2
175-179	32.98531898383459	33.8	31.0	36.0	30.0	36.6
180-183	31.665307162130837	32.333333333333336	31.0	34.0	29.333333333333332	34.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	1.0
11	2.0
12	4.0
13	8.0
14	14.0
15	18.0
16	23.0
17	27.0
18	44.0
19	77.0
20	73.0
21	98.0
22	134.0
23	172.0
24	208.0
25	284.0
26	356.0
27	481.0
28	592.0
29	885.0
30	1277.0
31	1700.0
32	2304.0
33	3217.0
34	4543.0
35	6434.0
36	9934.0
37	16682.0
38	31364.0
39	78374.0
40	50401.0
41	56932.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	19.901074010740107	30.17617676176762	23.478609786097863	26.444139441394416
2	18.93918939189392	31.36868868688687	22.237722377223772	27.45439954399544
3	20.258452584525845	31.556940569405693	19.958824588245882	28.22578225782258
4	22.262847628476283	30.170551705517056	19.750322503225032	27.81627816278163
5	24.69624240606015	29.51323783094577	18.03045076126903	27.76006900172504
6	23.875363753637536	31.099435994359943	20.62708127081271	24.39811898118981
7	22.08646933747342	27.058699997374948	26.12455514679687	24.730275518354766
8	22.846353463534637	29.785422854228543	29.208667086670864	18.159556595565956
9	22.70160201602016	31.046935469354693	29.567170671706716	16.684291842918427
10-14	22.795714731435705	30.27933446935151	27.20863533816541	19.716315461047376
15-19	22.699651996519965	29.843998439984396	26.70491704917049	20.751432514325145
20-24	22.598550985509856	29.233942339423397	26.6209912099121	21.546515465154652
25-29	22.388848888488884	29.271967719677193	26.414439144391444	21.924744247442472
30-34	22.323535956611437	29.231308860635362	26.796661373845414	21.64849380890778
35-39	22.600562770232656	29.520043923530352	26.30760197350862	21.571791332728367
40-44	22.176280332959816	29.538487261671655	26.738486341299918	21.54674606406861
45-49	22.1045988822954	29.45369178879024	26.571251353928478	21.870457974985882
50-54	22.154531946508172	29.644710330395274	26.539601534116986	21.661156188979565
55-59	22.07763079923224	29.49129893830757	26.42780751188118	22.003262750579008
60-64	22.051373807633386	29.455349454678487	26.50125167542387	21.99202506226426
65-69	22.212392696608163	29.27441808936831	26.633235418224594	21.879953795798937
70-74	22.334938274498512	29.38155634649318	26.044327849970365	22.23917752903794
75-79	22.078169388359246	29.64391501325213	26.3454244230433	21.932491175345326
80-84	21.815741349644615	29.50804904195231	26.28392804057402	22.392281567829055
85-89	22.140389625099104	29.215747347755507	26.135557065730357	22.508305961415033
90-94	22.179379514359177	29.400459190492246	25.84223156206552	22.57792973308306
95-99	22.16917782124439	29.33751492538902	25.8649276238355	22.628379629531093
100-104	22.0738630517837	29.26388447494953	26.171586526040464	22.490665947226308
105-109	21.962514298110676	29.211040113596027	25.96167120261902	22.86477438567428
110-114	21.946661224665156	29.01571377936264	25.94263879789051	23.094986198081692
115-119	21.953125811058726	29.079319504117766	25.458234276027664	23.509320408795844
120-124	22.25589943795979	28.388239320987964	25.678243140678713	23.677618100373536
125-129	22.479520200846157	28.59324003905342	24.881491468687525	24.045748291412895
130-134	21.70080472046274	28.170714942844754	25.763955713938262	24.36452462275424
135-139	21.760822002487227	28.62382239917839	25.370468168576704	24.24488742975768
140-144	21.71717887587065	28.599048059123522	25.313179476857744	24.370593588148083
145-149	21.630144361475516	28.04799341896262	25.521249594537203	24.800612625024662
150-154	22.312669588812355	27.9392324661027	24.329113820439467	25.418984124645476
155-159	22.244664237376366	28.128058302967208	24.51795939614784	25.10931806350859
160-164	21.4402348029185	27.983437024176155	23.54092171169123	27.035406461214112
165-169	21.797463881909547	27.129789572864322	22.340216708542712	28.73252983668342
170-174	23.401654309619445	25.18773715211075	20.7264991188658	30.684109419404002
175-179	26.896890573454062	21.909875763318592	15.803327016213938	35.38990664701341
180-183	21.7787913340935	21.505131128848344	15.758266818700115	40.95781071835804
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	11.0
15	17.0
16	10.5
17	21.5
18	55.5
19	98.5
20	121.0
21	194.0
22	257.5
23	345.0
24	491.0
25	783.5
26	1086.5
27	1454.5
28	1741.0
29	2033.0
30	2895.0
31	3744.5
32	4332.5
33	4995.0
34	6201.5
35	7633.5
36	8762.5
37	9744.5
38	10637.0
39	11650.5
40	12745.5
41	13512.0
42	14328.0
43	14809.0
44	14963.0
45	14748.0
46	13917.0
47	13584.0
48	13246.5
49	12188.5
50	11114.0
51	10359.5
52	9374.0
53	8285.0
54	7344.5
55	6373.0
56	5359.5
57	4536.5
58	3975.5
59	3469.5
60	2984.5
61	2566.0
62	2264.0
63	1772.5
64	1306.5
65	1011.5
66	713.5
67	557.0
68	477.5
69	360.0
70	203.0
71	132.5
72	92.5
73	64.0
74	54.0
75	34.5
76	22.0
77	11.0
78	6.0
79	10.5
80	7.0
81	2.0
82	1.5
83	1.0
84	1.0
85	0.5
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0015000150001500015
6	0.0
7	0.001125011250112501
8	0.0
9	0.0
10-14	6.000060000600007E-4
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.001584049675797833
35-39	0.0022876315388134817
40-44	0.0025309582207823883
45-49	0.001080045238466274
50-54	0.0
55-59	0.0
60-64	2.3963096830880443E-4
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	0.05050297304832289
105-109	0.06860847961392025
110-114	0.07432470504898185
115-119	0.06699025528919189
120-124	0.060152860979136545
125-129	0.05427337797876275
130-134	0.05326715804915375
135-139	0.04962260066212117
140-144	0.036556129244336634
145-149	0.025408117893667023
150-154	0.019640818531112283
155-159	0.02810245948562091
160-164	0.025485604077696653
165-169	0.017663336800580926
170-174	0.0
175-179	0.0
180-183	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
30-34	3656.0
35-39	1732.0
40-44	1317.0
45-49	1912.0
50-54	2562.0
55-59	3584.0
60-64	4169.0
65-69	6100.0
70-74	7186.0
75-79	8584.0
80-84	9588.0
85-89	10775.0
90-94	12516.0
95-99	12853.0
100-104	12739.0
105-109	12644.0
110-114	14326.0
115-119	14269.0
120-124	13149.0
125-129	13398.0
130-134	12430.0
135-139	12174.0
140-144	10812.0
145-149	10623.0
150-154	11185.0
155-159	9476.0
160-164	8943.0
165-169	8222.0
170-174	7679.0
175-179	5513.0
180-184	2548.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	24.206117061170612
#Duplication Level	Percentage of total
1	13.690261902619028
2	3.5227852278522787
3	3.4020340203402033
4	3.7395373953739535
5	4.046290462904629
6	4.396543965439655
7	4.48879488794888
8	4.6170461704617045
9	4.927549275492755
>10	53.035280352803525
>50	0.0907509075090751
>100	0.04312543125431254
>500	0.0
>1k	0.0
>5k	0.0
>10k+	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	0.0	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0	0.0
3	0.0011250112501125012	0.0	0.0	0.0	0.0	0.0
4	0.001875018750187502	0.0	0.0	0.0	0.0	0.0
5	0.02025020250202502	0.0	0.0	0.0	0.0	0.0
6	0.027750277502775027	0.0	0.0	0.0	0.0	0.0
7	0.03037530375303753	0.0	0.0	0.0	0.0	0.0
8	0.04387543875438754	0.0	0.0	0.0	0.0	0.0
9	0.06712567125671257	0.0	0.0	0.0011250112501125012	3.7500375003750037E-4	0.0
10-14	0.13927639276392761	0.0	0.0	0.0011250112501125012	0.0010500105001050011	0.0
15-19	0.16477664776647766	0.0	0.0	0.0011250112501125012	0.0011250112501125012	0.0
20-24	0.17692676926769269	0.0	0.0	0.0011250112501125012	0.0015000150001500015	0.0
25-29	0.1946269462694627	0.0	0.0	0.0011250112501125012	0.0017250172501725018	0.0
30-34	0.20872708727087272	0.0	0.0	0.0011250112501125012	0.001875018750187502	0.0
35-39	0.2137521375213752	0.0	0.0	0.0011250112501125012	0.0025500255002550024	0.0
40-44	0.21540215402154023	0.0	0.0	0.0011250112501125012	0.0026250262502625025	0.0
45-49	0.2167521675216752	0.0	0.0	0.0011250112501125012	0.003075030750307503	0.0
50-54	0.2167521675216752	0.0	0.0	0.0011250112501125012	0.0033750337503375034	0.0
55-59	0.21825218252182524	0.0	0.0	0.0011250112501125012	0.0033750337503375034	0.0
60-64	0.21825218252182524	0.0	0.0	0.0011250112501125012	0.0033750337503375034	0.0
65-69	0.21825218252182524	0.0	0.0	0.0011250112501125012	0.0033750337503375034	0.0
70-74	0.21825218252182524	0.0	0.0	0.0011250112501125012	0.0033750337503375034	0.0
75-79	0.21825218252182524	0.0	0.0	0.0011250112501125012	0.0033750337503375034	0.0
80-84	0.21915219152191517	0.0	0.0	0.0011250112501125012	0.0033750337503375034	0.0
85-89	0.21975219752197522	0.0	0.0	0.0011250112501125012	0.0033750337503375034	0.0
90-94	0.21975219752197522	0.0	0.0	0.0011250112501125012	0.0033750337503375034	0.0
95-99	0.21975219752197522	0.0	0.0	0.0011250112501125012	0.0033750337503375034	0.0
100-104	0.21975219752197522	0.0	0.0	0.0011250112501125012	0.0033750337503375034	0.0
105-109	0.2238772387723877	0.0	0.0	0.0011250112501125012	0.0033750337503375034	0.0
110-114	0.2238772387723877	0.0	0.0	0.0011250112501125012	0.0033750337503375034	0.0
115-119	0.2261272612726127	0.0	0.0	0.0011250112501125012	0.0033750337503375034	0.0
120-124	0.23512735127351272	0.0	0.0	0.0011250112501125012	0.0033750337503375034	0.0
125-129	0.23512735127351272	0.0	0.0	0.0011250112501125012	0.0033750337503375034	0.0
130-134	0.23512735127351272	0.0	0.0	0.0011250112501125012	0.0033750337503375034	0.0
135-139	0.23542735427354272	0.0	0.0	0.0011250112501125012	0.0033750337503375034	0.0
140-144	0.23962739627396273	0.0	0.0	0.0011250112501125012	0.0033750337503375034	0.0
145-149	0.24862748627486275	0.0	0.0	0.0011250112501125012	0.0033750337503375034	0.0
150-154	0.24982749827498277	0.0	0.0	0.0011250112501125012	0.0033750337503375034	0.0
155-159	0.25462754627546275	0.0	0.0	0.0011250112501125012	0.0033750337503375034	0.0
160-164	0.25462754627546275	0.0	0.0	0.0011250112501125012	0.0033750337503375034	0.0
165-169	0.25462754627546275	0.0	0.0	0.0011250112501125012	0.0033750337503375034	0.0
170-172	0.25462754627546275	0.0	0.0	0.0011250112501125012	0.0033750337503375034	0.0
>>END_MODULE
