FastQCFastQC Report
Wed 13 Sep 2023
stdin:SRR6235552

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6235552
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences606639
Total Bases48 Mbp
Sequences flagged as poor quality0
Sequence length30-200
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAAGAGCACACGTCTGAACTCCAGTCACCTATCAATCTCGTATGCCGTCT291284.801537652541297TruSeq Adapter, Index 2 (96% over 32bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTATCAATCTCGTATGC72061.1878563692739834TruSeq Adapter, Index 2 (97% over 37bp)
AGACGGAAGAGCACACGTCTGAACTCCAGTCACCTATCAATCTCGTATGC54950.9058105397114263TruSeq Adapter, Index 12 (96% over 33bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACCTATCAATCTCGTATGCCGT20180.3326525330550789TruSeq Adapter, Index 2 (97% over 34bp)
GAGACGGAAGAGCACACGTCTGAACTCCAGTCACCTATCAATCTCGTATG10510.17324965918775415TruSeq Adapter, Index 12 (96% over 33bp)
GACCGGAAGAGCACACGTCTGAACTCCAGTCACCTATCAATCTCGTATGC10360.1707770189519632TruSeq Adapter, Index 12 (97% over 35bp)
GAAGAGCACACGTCTGAACTCCAGTCACCTATCAATATCGTATGCCGTCT10250.1689637494457165TruSeq Adapter, Index 2 (96% over 32bp)
CAGACGGAAGAGCACACGTCTGAACTCCAGTCACCTATCAATCTCGTATG9910.16335909824459027TruSeq Adapter, Index 12 (96% over 33bp)
GACGGAAGAGCACACGTCTGAACTCCAGTCACCTATCAATCTCGTATGCC9900.1631942555622042TruSeq Adapter, Index 2 (97% over 34bp)
GATCCGGAAGAGCACACGTCTGAACTCCAGTCACCTATCAATCTCGTATG6700.11044459719866345TruSeq Adapter, Index 12 (96% over 33bp)

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)