FastQCFastQC Report
Wed 13 Sep 2023
stdin:SRR6235553

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6235553
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6327177
Total Bases747.7 Mbp
Sequences flagged as poor quality0
Sequence length30-200
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATG442350.699126956619042TruSeq Adapter, Index 10 (100% over 49bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATGC300090.4742873480542744TruSeq Adapter, Index 10 (100% over 50bp)
CAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTAT75280.11897881156161744TruSeq Adapter, Index 10 (100% over 48bp)
CAGGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCT70190.11093414962154528TruSeq Adapter, Index 10 (100% over 43bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)