FastQCFastQC Report
Wed 13 Sep 2023
stdin:SRR6235554

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6235554
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7458271
Total Bases869.2 Mbp
Sequences flagged as poor quality0
Sequence length30-200
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGTACCCGGAGGAAGAGAAAGAAACATCGATTTCCTTAGTAGCGGCGAG268210.35961417867492346No Hit
AAGTAGGGCGAGGAAGAGACATCAAACGAGATTCCGTAAGTAGTGGCGAG205960.2761497939669932No Hit
AGTACTGTGAAGGAAAGGTGAAAAGGACCCCGGGAGGGGAGTGAAAGAGA161710.21681968917460895No Hit
AAGGTTTCCAGGGTAAAGCTGATCTGCCCTGGGTAAGTCGGGGCCTAAGG137170.18391662088974778No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATG120850.1620348737663193TruSeq Adapter, Index 8 (100% over 49bp)
GTTAACCGAACGGCATGGGAAGGCCGGTCAGAGAGTGTGAGAACCACGTA115940.1554515785226898No Hit
GACTAAGAAGCCCCGGCTAACTACGTGCCAGCAGCCGCGGTAATACGTAG88960.11927697451594343No Hit
ATAACGGTCCTAAGGTAGCGAAATTCCTTGTCGGGTAAGTTCCGACCCGC87870.11781550978772425No Hit
AAGGTTTCCAGAGTAAAGCTGATCTGCTCTGGGTAAGTCGGGGCCTAAGG87130.11682332272452958No Hit
CAAGCAATAAGGGCACAGGGCGGATGCCTTGGCACTAAGAGCCGATGAAA80530.10797408675549602No Hit
ACTGTGAAGGAAAGGTGAAAAGGACCCCGGGAGGGGAGTGAAAGAGAACC80200.10753162495704432No Hit
CAACAAGGAAGTGACGGCTAACTACGTGCCAGCAGCCGCGGTAAAACGTA79860.10707575522530624No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)