FastQCFastQC Report
Wed 13 Sep 2023
stdin:SRR6235556

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6235556
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2835076
Total Bases528.1 Mbp
Sequences flagged as poor quality0
Sequence length60-200
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGACTGAGTGGCGGACGGGTGAGTAACGCGTGGGTAACCTGCCTTGTAC146940.5182929840328796No Hit
ATGGGTTGTAAACTTCTTTTATAAAGGAATAAAGTCGGGTATGCATACCC144250.5088047022372593No Hit
TGTGACTGAGTGGCGGACGGGTGAGTAACGCGTGGGTAACCTGCCTTGTA122360.4315933682201112No Hit
AATACGTATGGTGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCG114610.4042572403702758No Hit
ATCAGCAGGGAAGAAAATGACGGTACCTGACTAAGAAGCCCCGGCTAACT112570.3970616660717385No Hit
AATACGTAGGGGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCG90020.31752235213447544No Hit
CATGGGTTGTAAACTTCTTTTATAAAGGAATAAAGTCGGGTATGCATACC86210.304083558959266No Hit
AATGGGTTGTAAACTTCTTTTATAAAGGAATAAAGTCGGGTATGCATACC82800.2920556627053384No Hit
TATGGGTTGTAAACTTCTTTTATAAAGGAATAAAGTCGGGTATGCATACC81430.28722334074994815No Hit
GCTTTGAGATGGCCTCGCGTCCGATTAGCTAGTTGGTGAGGTAACGGCCC80120.28260265333275014No Hit
GACTAAGAAGCCCCGGCTAACTACGTGCCAGCAGCCGCGGTAATACGTAG79470.2803099458356672No Hit
ATCAGCAGGGAAGAAAATGACGGTACCTGACTAAGAAGCACCGGCTAAAT75230.2653544384700798No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATG63110.22260426175524042TruSeq Adapter, Index 3 (100% over 49bp)
AATGCGAGTGGTAGGGGAGCGTTCTGTAAGCCTGCGAAGGCGGATCTGCG55510.1957972202508857No Hit
AAGTAGGGCGAGGAAGAGACATCAAACGAGATTCCGTAAGTAGTGGCGAG49030.1729406901261201No Hit
ATACCCGTTTGCATGTACTTTATGAATAAGGATCGGCTAACTCCGTGCCA44180.1558335649555779No Hit
ATCAGCAGGGAAGATAATGACGGTACCTGACTAAGAAGCACCGGCTAAAT41500.14638055558298965No Hit
ACTGGGTGTAAAGGGAGCGCAGGCGGTGCGGCAAGTCTGATGTGAAAGCC41010.14465220685441943No Hit
AATGGATTGGGTAGGGGAGCGTTGTATACGCGGAGAAACAGTAGCGTAAG39660.13989042974509325No Hit
ACCTGACTAAGAAGCCCCGGCTAACTACGTGCCAGCAGCCGCGGTAATAC39350.13879698463109985No Hit
TTGTGACTGAGTGGCGGACGGGTGAGTAACGCGTGGGTAACCTGCCTTGT38260.13495229052060687No Hit
GACTAAGAAGCACCGGCTAAATACGTGCCAGCAGCCGCGGTAATACGTAT36670.1293439752585116No Hit
AGTGGCGGACGGGTGAGTAACGCGTGGGTAACCTGCCTTATACAGGGGGA33890.11953824165560288No Hit
CCTAGTGTGAAAAACTCCGGTGGTACAGGATGGGCCCGCGTCTGATTAGC31660.1116724913194567No Hit
TGCAGTATTGGGTTTCTAACCAGCCGCCGTGACCCGGCGGTGGGACAATG30600.10793361447805985No Hit
TTGCATGTACTTTATGAATAAGGATCGGCTAACTCCGTGCCAGCAGCCGC30110.10620526574948962No Hit
AAAACGTAGGTCACAAGCGTTGTCCGGAATTACTGGGTGTAAAGGGAGCG29870.10535872759672052No Hit
ATGGGTTGTAAACTTCTTTTATACGGGAATAAAGTGAGGCACGTGTGCCT29640.10454746186698345No Hit
ACGTAGGGGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGTAG28860.10179621287048389No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)