FastQCFastQC Report
Wed 13 Sep 2023
stdin:SRR6235558

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6235558
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3172399
Total Bases166.7 Mbp
Sequences flagged as poor quality0
Sequence length30-200
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCAGAAATCTCGTATGCCGTCTTCTGCTTGAAAAAA116350.3667571449871217TruSeq Adapter, Index 25 (96% over 27bp)
GCAGAAATCTCGTATGCCGTCTTCTGCTTGAAAAAAA108600.34232768324539253TruSeq Adapter, Index 25 (96% over 27bp)
GCAGAAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAA56110.17686930301011947TruSeq Adapter, Index 25 (96% over 27bp)
GCAGAAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA38040.11990925479424247TruSeq Adapter, Index 25 (96% over 27bp)
GCAGAAATCTCGTATGCCGTCTTCTGCTTGAAAAA34020.10723745657466163TruSeq Adapter, Index 25 (96% over 27bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)