FastQCFastQC Report
Wed 13 Sep 2023
stdin:SRR6235559

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6235559
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences264393
Total Bases21.7 Mbp
Sequences flagged as poor quality0
Sequence length30-200
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAAGAGCACACGTCTGAACTCCAGTCACACGCATATCTCGTATGCCGTCT4117715.57416421766083TruSeq Adapter, Index 15 (96% over 31bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACGCATATCTCGTATGC75302.848033041721982TruSeq Adapter, Index 15 (97% over 36bp)
AGACGGAAGAGCACACGTCTGAACTCCAGTCACACGCATATCTCGTATGC33411.2636491888968315TruSeq Adapter, Index 5 (96% over 33bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACACGCATATCTCGTATGCCGT16150.6108331158540506TruSeq Adapter, Index 15 (96% over 33bp)
GACGGAAGAGCACACGTCTGAACTCCAGTCACACGCATATCTCGTATGCC7770.29388070032111285TruSeq Adapter, Index 15 (96% over 33bp)
CAGACGGAAGAGCACACGTCTGAACTCCAGTCACACGCATATCTCGTATG6330.2394163234276248TruSeq Adapter, Index 5 (96% over 33bp)
GACCGGAAGAGCACACGTCTGAACTCCAGTCACACGCATATCTCGTATGC6310.23865987374854855TruSeq Adapter, Index 5 (97% over 35bp)
GAGACGGAAGAGCACACGTCTGAACTCCAGTCACACGCATATCTCGTATG6170.23336472599501498TruSeq Adapter, Index 5 (96% over 33bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACACGCATATCTCGTATGCC4770.18041324845967935TruSeq Adapter, Index 15 (97% over 35bp)
GATCCGGAAGAGCACACGTCTGAACTCCAGTCACACGCATATCTCGTATG4740.179278573941065TruSeq Adapter, Index 5 (96% over 33bp)
GAAGAGCACACGTCTGAACTCCAGTCACACGCATATCTCGTATGCCGGCT4540.17171407715030276TruSeq Adapter, Index 15 (96% over 31bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACGCATATCTCGTATG3280.12405774736850068TruSeq Adapter, Index 15 (97% over 36bp)
GAAGAGCACACGTCTGAACTCCAGTCAAACGCATATCTCGTATGCCGTCT3110.11762792509635278TruSeq Adapter, Index 5 (96% over 30bp)
GCACACCGGAAGAGCACACGTCTGAACTCCAGTCACACGCATATCTCGTA3020.11422390154050978TruSeq Adapter, Index 15 (96% over 33bp)
AGCACACCGGAAGAGCACACGTCTGAACTCCAGTCACACGCATATCTCGT2890.10930697862651431TruSeq Adapter, Index 15 (96% over 33bp)

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)