##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	SRR6235559
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	264393
Total Bases	21.7 Mbp
Sequences flagged as poor quality	0
Sequence length	30-200
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.801757232604494	31.0	30.0	34.0	25.0	41.0
2	31.962926401228476	31.0	31.0	34.0	25.0	41.0
3	32.004550044819645	31.0	31.0	34.0	25.0	41.0
4	34.646843902826475	37.0	35.0	37.0	27.0	41.0
5	34.43628235240721	37.0	35.0	37.0	26.0	41.0
6	34.25332365077744	37.0	35.0	37.0	25.0	41.0
7	34.161433926011654	37.0	35.0	37.0	25.0	41.0
8	34.34804249734297	37.0	35.0	37.0	25.0	41.0
9	35.35947245199381	39.0	35.0	39.0	25.0	41.0
10-14	35.34588964155632	39.2	34.8	39.4	25.0	41.0
15-19	35.93896434474438	40.0	35.2	41.0	25.0	41.0
20-24	35.98110691281539	40.0	35.0	41.0	25.0	41.0
25-29	35.5773178563729	39.4	34.0	41.0	24.0	41.0
30-34	35.6017961055133	39.0	33.8	41.0	24.4	41.0
35-39	35.3245701492222	38.6	33.2	41.0	25.0	41.0
40-44	35.72500190610541	38.6	33.8	41.0	25.2	41.0
45-49	35.626525781794776	38.6	33.4	41.0	25.6	41.0
50-54	35.726390387514485	38.6	33.4	41.0	25.8	41.0
55-59	35.71071565371655	38.4	33.4	41.0	26.6	41.0
60-64	35.34381480920267	36.8	32.8	41.0	27.4	41.0
65-69	35.063597916157214	36.0	32.0	41.0	27.0	41.0
70-74	35.043715573392554	35.4	32.0	41.0	26.2	41.0
75-79	35.23017655434512	36.4	32.2	41.0	26.8	41.0
80-84	35.62653648547828	37.2	33.4	41.0	27.0	41.0
85-89	35.66106300835857	38.8	33.8	41.0	26.2	41.0
90-94	35.5299569064194	39.0	33.0	41.0	25.0	41.0
95-99	35.12326502614072	37.8	32.8	41.0	24.6	41.0
100-104	33.09091996953954	35.0	30.6	38.6	23.2	40.2
105-109	33.51766600215073	36.4	31.2	38.2	23.0	40.0
110-114	33.51650070916852	37.0	31.4	39.2	21.4	40.2
115-119	33.39337739004335	37.0	30.6	40.0	20.6	41.0
120-124	33.778906989111825	37.0	31.0	40.0	22.4	41.0
125-129	33.851831962091055	36.2	31.0	39.6	23.8	41.0
130-134	33.88303869174352	35.8	31.0	39.0	24.6	41.0
135-139	34.1152853495683	35.2	32.0	39.0	25.6	41.0
140-144	34.72127873493562	36.0	32.8	39.0	27.2	41.0
145-149	35.024600064081056	36.0	33.0	39.6	28.2	41.0
150-154	34.8248092477465	36.8	33.0	39.2	27.4	40.6
155-159	34.93411026590958	37.0	33.0	39.6	27.0	41.0
160-164	34.45693411993141	36.0	32.4	39.0	26.4	41.0
165-169	34.44705971467087	35.0	33.0	38.6	28.0	40.0
170-174	33.86081458426778	35.0	32.4	37.0	27.2	39.2
175-179	33.351436206176814	35.0	32.0	36.0	27.0	38.4
180-184	32.93879297700626	35.0	32.0	35.0	27.0	37.0
185-189	33.03635346859713	34.4	32.0	35.0	28.2	36.0
190-194	33.383772559353154	34.8	32.6	35.0	30.0	35.4
195-199	33.170456473585766	35.0	32.8	35.0	29.0	35.0
200	33.119565217391305	35.0	33.0	35.0	29.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	2.0
10	1.0
11	1.0
12	3.0
13	3.0
14	5.0
15	26.0
16	30.0
17	257.0
18	2226.0
19	5563.0
20	6751.0
21	6198.0
22	4994.0
23	4138.0
24	3871.0
25	3938.0
26	4657.0
27	5482.0
28	6543.0
29	7394.0
30	8432.0
31	8700.0
32	9387.0
33	10180.0
34	11322.0
35	13012.0
36	16096.0
37	20668.0
38	22789.0
39	20503.0
40	16445.0
41	44775.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.61191862114352	22.119345065867858	14.01171740552889	22.257018907459727
2	20.917346525815734	42.327141792710094	14.514378217275043	22.241133464199127
3	20.03532620001286	38.57931185772694	16.75422571701974	24.631136225240457
4	37.38591706979435	19.008589551081542	12.751568699388404	30.853924679735695
5	24.672347861608817	36.69590477379237	11.458085112016372	27.17366225258245
6	44.53442061189678	20.74125064771494	13.256880907443898	21.46744783294439
7	18.342877459472135	28.16714954649641	16.141928846459344	37.34804414757211
8	18.42977207222882	48.02444947084143	20.036159799078607	13.509618657851139
9	23.036886858111174	24.98676127938996	19.318697612491302	32.65765425000757
10-14	24.617125127700632	29.911006848537593	21.395966551893753	24.075901471868026
15-19	22.679664169785877	32.092385093788174	22.31338099050842	22.914569745917532
20-24	20.12437555031756	27.604836440326665	24.389090777055955	27.881697232299818
25-29	17.077635795315285	30.61049264854953	24.679631686128317	27.63223987000687
30-34	18.11531094930205	27.22118925662464	23.051313732311296	31.612186061762017
35-39	15.393895815502557	27.379665179389907	26.59730215328699	30.629136851820547
40-44	23.07872340022324	26.750435393977067	28.202822527919224	21.96801867788047
45-49	19.90734486230417	20.27023429048707	28.61895919761992	31.203461649588842
50-54	21.75720761891579	16.720881993115537	35.71158124868957	25.810329139279105
55-59	21.498521210028184	19.234166839516806	33.06414337050401	26.203168579950997
60-64	15.588570583900898	38.19790502511775	26.776439707321664	19.437084683659688
65-69	19.348084472204555	37.574321175142266	25.064563975355895	18.01303037729728
70-74	20.339203543808093	42.216981525431926	20.10570715216126	17.338107778598722
75-79	23.890668212619154	34.01944478763745	22.128662529343003	19.961224470400396
80-84	26.72464020535405	28.274540783638024	23.761479113145853	21.23933989786207
85-89	26.48289780711931	29.547414642261437	23.916146063628123	20.053541486991126
90-94	28.782787136005723	30.424203060211134	22.211475556434117	18.581534247349023
95-99	27.28487480450066	30.222299831452997	22.787757312561055	19.705068051485288
100-104	30.951182545953532	25.059401458253078	24.80829848916433	19.18111750662906
105-109	28.782203103098265	28.087389433998744	24.192807772246123	18.93759969065687
110-114	29.231686839938543	26.768105700597093	25.631940977782385	18.368266481681978
115-119	25.8993004672768	25.070851881549476	27.410000829485444	21.61984682168828
120-124	24.338348814950365	27.01942505931666	27.139341887644903	21.50288423808808
125-129	24.039990732705306	28.29430728935557	27.208148936420862	20.457553041518263
130-134	21.34132288545748	33.01299751703505	24.146942822231015	21.498736775276452
135-139	17.94377902713273	36.73576142752383	26.058176484967	19.262283060376436
140-144	17.569460860340286	38.96207976107424	25.365682097288445	18.102777281297026
145-149	18.49261222761999	40.724465318792966	22.835238229622313	17.94768422396474
150-154	20.53364076703603	34.05795899409462	25.02654103908168	20.381859199787673
155-159	22.46744131638462	28.201278951883335	27.028581455197692	22.302698276534354
160-164	21.657687346204295	27.76073453370768	27.934178148272608	22.647399971815414
165-169	23.12210686616952	27.952960514388618	27.452591885326143	21.472340734115715
170-174	22.20770389728137	27.563632484900204	27.16497113371822	23.063692484100212
175-179	22.185709845019762	28.355314771230223	27.55598975679796	21.902985626952052
180-184	21.055030934549006	28.433734939759038	29.049169651579287	21.462064474112665
185-189	21.337888045641034	27.979785529397265	29.224701097004807	21.457625327956894
190-194	20.79617607191268	29.81023043447243	29.2715987729186	20.1219947206963
195-199	23.84981491274458	26.282390269698574	28.609201480698044	21.258593336858805
200	20.380434782608695	30.434782608695656	26.358695652173914	22.82608695652174
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	39.0
1	38.5
2	43.0
3	49.0
4	59.0
5	78.5
6	91.5
7	114.0
8	172.5
9	218.5
10	250.0
11	309.5
12	379.0
13	457.0
14	556.5
15	688.0
16	785.0
17	869.5
18	979.0
19	1072.5
20	1212.0
21	1411.0
22	1554.5
23	1672.0
24	1876.0
25	2111.3333333333335
26	2288.833333333334
27	2535.333333333334
28	2945.1666666666674
29	3268.333333333334
30	3619.3333333333335
31	4008.1666666666674
32	4271.833333333331
33	4661.833333333331
34	5215.499999999997
35	5736.499999999995
36	6223.499999999996
37	6760.1666666666615
38	7514.333333333327
39	8131.166666666661
40	8549.666666666686
41	9035.166666666688
42	9529.500000000016
43	10610.333333333348
44	12705.500000000015
45	19885.166666666624
46	30004.999999999964
47	35949.50000000005
48	32846.50000000006
49	26544.499999999975
50	23933.83333333331
51	21792.833333333314
52	19440.66666666665
53	16428.0
54	13262.166666666675
55	10907.833333333338
56	9184.500000000007
57	7715.499999999995
58	6515.999999999996
59	5556.833333333332
60	4709.333333333331
61	4041.333333333334
62	3590.333333333334
63	3058.666666666667
64	2505.6666666666674
65	2027.6666666666663
66	1654.6666666666665
67	1327.6666666666665
68	1050.1666666666665
69	839.8333333333334
70	698.5
71	590.0
72	483.5
73	379.0
74	289.0
75	232.0
76	177.0
77	128.0
78	103.5
79	82.0
80	58.5
81	40.0
82	27.0
83	25.0
84	27.0
85	20.5
86	12.5
87	8.0
88	5.5
89	5.0
90	6.5
91	7.5
92	4.5
93	1.0
94	1.5
95	5.5
96	8.5
97	8.0
98	5.5
99	3.5
100	4.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	7.564496790762236E-4
5	0.004538698074457342
6	7.564496790762236E-4
7	0.0026475738767667827
8	0.00416047323491923
9	0.0064298222721479005
10-14	0.03895715847242552
15-19	0.04410101629014384
20-24	7.564496790762236E-5
25-29	0.003706603427473496
30-34	0.03941042641601742
35-39	0.04737722371256864
40-44	0.05426449823332598
45-49	0.03125
50-54	0.035508942861125437
55-59	0.05135485256000783
60-64	0.05551265263181457
65-69	0.012889883566106689
70-74	0.0
75-79	0.0032311897240563975
80-84	0.0029027395641329206
85-89	0.001094458319743809
90-94	0.0
95-99	0.001012284067154925
100-104	0.037384333434524006
105-109	0.04016221912869122
110-114	0.03758789410582936
115-119	0.042838388723830584
120-124	0.02932884601989229
125-129	0.027087717094312364
130-134	0.036513642627069645
135-139	0.06351377760406489
140-144	0.06568447253640014
145-149	0.02619805731328846
150-154	0.026533996683250415
155-159	0.12267549410962905
160-164	0.1666612555869398
165-169	0.010876395804128197
170-174	0.0
175-179	0.0
180-184	0.0
185-189	0.0
190-194	0.0
195-199	0.0
200	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
30-34	33891.0
35-39	15270.0
40-44	9331.0
45-49	7014.0
50-54	5891.0
55-59	8128.0
60-64	30162.0
65-69	28782.0
70-74	15396.0
75-79	11956.0
80-84	5021.0
85-89	5844.0
90-94	6513.0
95-99	5246.0
100-104	8032.0
105-109	4447.0
110-114	4210.0
115-119	3555.0
120-124	2894.0
125-129	5033.0
130-134	4308.0
135-139	6688.0
140-144	4891.0
145-149	5604.0
150-154	4773.0
155-159	2206.0
160-164	2088.0
165-169	1603.0
170-174	1589.0
175-179	1284.0
180-184	987.0
185-189	1369.0
190-194	10002.0
195-199	17.0
200-201	368.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	42.11672068881322
#Duplication Level	Percentage of total
1	33.92065067407931
2	6.5166573926309725
3	4.237614737678267
4	3.3637609197887692
5	2.9072859714970605
6	2.598537125754826
7	2.317019914036896
8	2.106992728210492
9	1.8184153748850116
>10	12.550384516242637
>50	1.0770194089178031
>100	2.927778970420081
>500	1.1165045198531671
>1k	2.081789465911323
>5k	3.1630094185456543
>10k+	17.29657886154773
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAAGAGCACACGTCTGAACTCCAGTCACACGCATATCTCGTATGCCGTCT	41177	15.57416421766083	TruSeq Adapter, Index 15 (96% over 31bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACGCATATCTCGTATGC	7530	2.848033041721982	TruSeq Adapter, Index 15 (97% over 36bp)
AGACGGAAGAGCACACGTCTGAACTCCAGTCACACGCATATCTCGTATGC	3341	1.2636491888968315	TruSeq Adapter, Index 5 (96% over 33bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACACGCATATCTCGTATGCCGT	1615	0.6108331158540506	TruSeq Adapter, Index 15 (96% over 33bp)
GACGGAAGAGCACACGTCTGAACTCCAGTCACACGCATATCTCGTATGCC	777	0.29388070032111285	TruSeq Adapter, Index 15 (96% over 33bp)
CAGACGGAAGAGCACACGTCTGAACTCCAGTCACACGCATATCTCGTATG	633	0.2394163234276248	TruSeq Adapter, Index 5 (96% over 33bp)
GACCGGAAGAGCACACGTCTGAACTCCAGTCACACGCATATCTCGTATGC	631	0.23865987374854855	TruSeq Adapter, Index 5 (97% over 35bp)
GAGACGGAAGAGCACACGTCTGAACTCCAGTCACACGCATATCTCGTATG	617	0.23336472599501498	TruSeq Adapter, Index 5 (96% over 33bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACACGCATATCTCGTATGCC	477	0.18041324845967935	TruSeq Adapter, Index 15 (97% over 35bp)
GATCCGGAAGAGCACACGTCTGAACTCCAGTCACACGCATATCTCGTATG	474	0.179278573941065	TruSeq Adapter, Index 5 (96% over 33bp)
GAAGAGCACACGTCTGAACTCCAGTCACACGCATATCTCGTATGCCGGCT	454	0.17171407715030276	TruSeq Adapter, Index 15 (96% over 31bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACGCATATCTCGTATG	328	0.12405774736850068	TruSeq Adapter, Index 15 (97% over 36bp)
GAAGAGCACACGTCTGAACTCCAGTCAAACGCATATCTCGTATGCCGTCT	311	0.11762792509635278	TruSeq Adapter, Index 5 (96% over 30bp)
GCACACCGGAAGAGCACACGTCTGAACTCCAGTCACACGCATATCTCGTA	302	0.11422390154050978	TruSeq Adapter, Index 15 (96% over 33bp)
AGCACACCGGAAGAGCACACGTCTGAACTCCAGTCACACGCATATCTCGT	289	0.10930697862651431	TruSeq Adapter, Index 15 (96% over 33bp)
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	0.21029301078319018	0.0	0.0	0.0	0.004538698074457342	0.01475076874198636
2	0.2556799915277636	0.0	0.0	0.0	0.009833845827990908	0.015128993581524473
3	0.3362418823493814	0.0	0.0	0.0	0.011724970025681467	0.017398342618753143
4	0.4213424712454566	0.0	0.0	0.0	0.013994319062910137	0.01853301713736748
5	0.7227876683573317	0.0	0.0	0.0	0.017776567458291256	0.019289466816443702
6	1.2273396043011728	0.0	0.0	0.0	0.023828164890901046	0.019667691655981815
7	2.2141282106561064	0.0	0.0	0.0	0.03593135975612062	0.021180591014134262
8	2.919517536394685	0.0	0.0	0.0	0.04463053106549719	0.023449940051362934
9	3.162337883378153	0.0	0.0	0.0	0.05408615205394999	0.02496283940951538
10-14	3.7429130120691547	0.0	0.0	0.0	0.07950286127091111	0.027988638125820274
15-19	3.912130805278506	0.0	0.0	0.0	0.15310541504502767	0.032300401296554745
20-24	4.089745189925603	0.0	0.0	0.0	0.2581006305008075	0.03835199872916454
25-29	4.296558532185044	0.0	0.0	0.0	0.37535033075762214	0.04069699273430083
30-34	4.460708112544583	0.0	0.0	0.0	0.4535672275741037	0.04266376189989902
35-39	4.5524654586165285	0.0	0.0	0.0	0.4957014746986494	0.04349585654688286
40-44	4.609047894611431	0.0	0.0	0.0	0.5123433676383262	0.04349585654688286
45-49	4.65330020083739	0.0	0.0	0.0	0.52081560404398	0.04349585654688286
50-54	4.701864270234083	0.0	0.0	0.0	0.5286826807063727	0.04349585654688286
55-59	4.757765901517817	0.0	0.0	0.0	0.5438873192558048	0.04349585654688286
60-64	4.8328057096821775	0.0	0.0	0.0	0.5941912229143738	0.04349585654688286
65-69	5.185159970195882	0.0	0.0	0.0	0.6506980139413676	0.04357150151479048
70-74	6.717727020004311	0.0	0.0	0.0	0.7115922131070036	0.043874081386420974
75-79	7.456173196718521	0.0	0.0	0.0	0.7207452542238258	0.043874081386420974
80-84	8.263531939196575	0.0	0.0	0.0	0.7237710529401309	0.043874081386420974
85-89	9.255766983240857	0.0	0.0	0.0	0.730352165148094	0.043874081386420974
90-94	9.520524370917537	0.0	0.0	0.0	0.7344369934151056	0.043874081386420974
95-99	9.567424251020261	0.0	0.0	0.0	0.7348908632225513	0.043874081386420974
100-104	9.604263350391275	0.0	0.0	0.0	0.7356473129016274	0.043874081386420974
105-109	9.648137431777695	0.0	0.0	0.0	0.736328117612796	0.043874081386420974
110-114	9.681572507592865	0.0	0.0	0.0	0.7379166619388562	0.04410101629014383
115-119	9.702677453639089	0.0	0.0	0.0	0.7395808512328239	0.04425230622595908
120-124	9.7176551572848	0.0	0.0	0.0	0.7416989103342373	0.04425230622595908
125-129	9.725446588979285	0.0	0.0	0.0	0.7454811587296184	0.04425230622595908
130-134	9.731271251508172	0.0	0.0	0.0	0.7485826024138309	0.04425230622595908
135-139	9.736188174422166	0.0	0.0	0.0	0.7523648508092121	0.04425230622595908
140-144	9.739440908042194	0.0	0.0	0.0	0.7564496790762236	0.04425230622595908
145-149	9.741105097336163	0.0	0.0	0.0	0.7572061287552999	0.04425230622595908
150-154	9.743601381277113	0.0	0.0	0.0	0.7572061287552999	0.04425230622595908
155-159	9.748140079351572	0.0	0.0	0.0	0.7572817737232075	0.04425230622595908
160-164	9.752603132458121	0.0	0.0	0.0	0.7579625784343761	0.04425230622595908
165-169	9.753057002265567	0.0	0.0	0.0	0.7583408032739142	0.04425230622595908
170-174	9.75328393716929	0.0	0.0	0.0	0.7583408032739142	0.04425230622595908
175-179	9.753662162008828	0.0	0.0	0.0	0.7583408032739142	0.04425230622595908
180-184	9.754721191559533	0.0	0.0	0.0	0.7583408032739142	0.04425230622595908
185-189	9.754796836527442	0.0	0.0	0.0	0.7583408032739142	0.04425230622595908
>>END_MODULE
