##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	SRR6235561
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	95243
Total Bases	6 Mbp
Sequences flagged as poor quality	0
Sequence length	30-200
%GC	37
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	26.354493243597954	28.0	16.0	34.0	10.0	41.0
2	26.254034417227512	30.0	16.0	34.0	10.0	41.0
3	26.169912749493403	30.0	16.0	34.0	10.0	41.0
4	28.324884768434426	33.0	19.0	37.0	10.0	41.0
5	28.204004493768572	33.0	19.0	37.0	10.0	41.0
6	27.9546423359197	32.0	17.0	37.0	10.0	41.0
7	27.91825121006268	32.0	17.0	37.0	10.0	41.0
8	27.782713690244954	32.0	17.0	37.0	10.0	41.0
9	28.352298856608886	32.0	17.0	39.0	10.0	41.0
10-14	28.15008767048497	32.0	17.0	39.4	10.0	41.0
15-19	28.022311350965424	32.0	17.2	41.0	10.0	41.0
20-24	28.006860346692143	32.0	17.2	41.0	10.0	41.0
25-29	28.270264481379208	32.0	17.0	40.6	9.8	41.0
30-34	29.651667441680523	33.2	21.2	40.8	9.0	41.0
35-39	31.08979899765726	34.8	24.8	41.0	9.0	41.0
40-44	32.57937385028502	37.6	28.4	41.0	12.8	41.0
45-49	34.10502323082701	39.6	30.6	41.0	16.2	41.0
50-54	35.196139174998144	40.2	32.8	41.0	21.0	41.0
55-59	36.23838472443295	41.0	34.8	41.0	24.6	41.0
60-64	37.06206683099539	41.0	36.0	41.0	28.0	41.0
65-69	37.34923463011115	41.0	36.2	41.0	29.4	41.0
70-74	37.45067108625229	41.0	36.0	41.0	30.0	41.0
75-79	37.212717690573314	40.8	35.2	41.0	30.0	41.0
80-84	37.19325015269267	41.0	35.0	41.0	30.6	41.0
85-89	36.99741029134573	40.4	35.0	41.0	31.0	41.0
90-94	36.794979371665626	39.0	35.0	41.0	31.0	41.0
95-99	36.53840527047619	37.2	34.4	41.0	31.0	41.0
100-104	35.33739484913922	35.0	32.8	39.8	30.4	41.0
105-109	36.21165429027515	37.4	35.2	39.2	31.0	41.0
110-114	36.89138912157961	39.0	36.2	39.4	31.0	41.0
115-119	37.38075880086707	39.4	36.8	41.0	31.0	41.0
120-124	37.393363402186814	40.0	36.8	41.0	31.2	41.0
125-129	37.30243540371568	39.2	36.2	41.0	31.0	41.0
130-134	37.32485676608526	39.0	36.4	41.0	31.2	41.0
135-139	37.3494108224054	39.0	36.8	41.0	31.0	41.0
140-144	37.11425861194889	39.0	36.0	41.0	30.6	41.0
145-149	36.941952210527674	39.0	35.8	41.0	30.6	41.0
150-154	36.56382723533743	38.6	35.0	40.4	30.0	41.0
155-159	36.864207554537494	39.0	35.0	41.0	30.2	41.0
160-164	37.05608711389019	39.0	35.0	41.0	31.0	41.0
165-169	36.473221487896296	38.6	34.6	40.8	30.2	41.0
170-174	36.04264786923325	37.6	34.0	40.0	29.6	41.0
175-179	35.81451939601298	37.0	34.0	39.4	30.0	41.0
180-184	35.36021075216829	36.0	34.0	38.8	30.0	41.0
185-189	34.732541004698625	35.0	34.0	37.2	29.4	40.2
190-194	34.32338078547567	35.0	34.0	36.4	29.0	39.2
195-199	34.22341417135466	35.0	34.0	36.0	30.0	38.8
200	34.28156505040806	35.0	34.0	36.0	31.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	2.0
11	4.0
12	8.0
13	4.0
14	8.0
15	9.0
16	18.0
17	242.0
18	3084.0
19	9145.0
20	11154.0
21	9646.0
22	7270.0
23	5292.0
24	3971.0
25	2906.0
26	2317.0
27	2004.0
28	1569.0
29	1312.0
30	1141.0
31	925.0
32	927.0
33	928.0
34	1027.0
35	1287.0
36	1735.0
37	2327.0
38	3527.0
39	4631.0
40	4687.0
41	12135.0
>>END_MODULE
>>Per base sequence content	pass
#Base	G	A	T	C
1	20.7710802893651	31.787112963682368	28.37163886059868	19.070167886353854
2	22.462459309041268	30.624803108264203	28.84700199516959	18.06573558752494
3	20.078351468302316	29.91114565390917	29.163340755366974	20.847162122421544
4	19.883636669152168	29.938352639704263	30.827880990138524	19.350129701005052
5	19.860740616270036	29.98067592261967	29.617299250141784	20.541284210968513
6	19.943708122413828	30.197021571551595	30.291541515259716	19.567728790774854
7	19.451329664328025	30.485653068940888	30.340713355459396	19.722303911271688
8	19.63473676472133	30.535922452451718	29.704155683214484	20.125185099612473
9	19.538600470430108	30.864625336021508	28.970304099462364	20.62647009408602
10-14	19.59025705531908	31.00518478541624	29.97998744227753	19.424570716987155
15-19	19.319883322168156	31.27369099694865	30.449008049426375	18.957417631456813
20-24	19.017488890942232	31.524313926432416	30.66708946886524	18.79110771376011
25-29	18.549965558374353	32.132566657146214	31.0640362225097	18.253431561969723
30-34	18.47581637681193	32.273244232291695	31.243358874178835	18.00758051671754
35-39	18.433566178593022	32.27393297824178	31.24815794990839	18.044342893256804
40-44	18.372378822006073	31.720469708893688	31.515776496722196	18.391374972378046
45-49	18.508853144773262	31.447252910672223	31.525926210201625	18.51796773435289
50-54	18.4949408100398	30.97351683454712	31.65225115447469	18.879291200938383
55-59	18.640694419476617	30.504763861424276	31.734251007986852	19.120290711112254
60-64	18.767871804309053	31.184705982276835	31.326972622519182	18.720449590894937
65-69	18.67315061325618	31.95010849325187	31.035995137040057	18.340745756451888
70-74	18.713852476675008	31.357406824320964	31.384750509586862	18.543990189417165
75-79	19.083156588546938	31.039611366816573	31.10825581497681	18.768976229659682
80-84	18.909563361328942	30.729896524523227	31.611526147278546	18.74901396686929
85-89	18.872479548053523	31.07425253877806	31.566858561473126	18.486409351695286
90-94	18.58681009039397	31.676967534270524	31.370512438169087	18.36570993716642
95-99	18.32772287979151	31.755890976218915	31.622325985449017	18.29406015854056
100-104	17.751283563200207	32.21060503645751	31.9761839702012	18.061927430141083
105-109	17.842694438577393	32.28484622008481	31.94527937090773	17.927179970430064
110-114	17.775458884308122	32.43751817575312	32.17578733085858	17.61123560908018
115-119	17.721747285647297	32.0510045810338	32.608303574649206	17.618944558669696
120-124	17.67181459828995	32.652866909146304	31.96160950621751	17.713708986346237
125-129	17.767383973020717	32.088084149670784	32.4032439376907	17.741287939617795
130-134	17.814741921147863	32.18419994497704	32.48047743000445	17.520580703870653
135-139	17.943543866729446	32.692092105558174	31.952198381202212	17.412165646510168
140-144	17.704381638846737	32.53272003034902	32.539833080424884	17.223065250379364
145-149	17.836095300458368	32.471163048405785	32.002719991940765	17.690021659195086
150-154	17.830911917653996	32.01093657856645	32.579209778587895	17.578941725191658
155-159	17.830719570371638	32.75916245322364	32.07358528294341	17.336532693461308
160-164	17.597050399171184	32.51264549942105	32.4120909257115	17.478213175696265
165-169	17.90498595046723	32.310658040907015	32.29105404169117	17.493301966934588
170-174	17.793544308243476	32.61915633451583	32.22928594007938	17.358013417161324
175-179	17.524109722691282	32.54531126871553	32.350181995572065	17.580397013021127
180-184	17.475223785166243	32.55274936061381	32.17311381074169	17.79891304347826
185-189	18.109056668378702	32.1391884951207	32.19054956343092	17.56120527306968
190-194	17.20342805593144	33.22056833558864	32.28236355435273	17.2936400541272
195-199	17.489980426880418	32.71507130207848	32.69643023580949	17.098518035231617
200	16.658665386461834	32.8372539606337	32.78924627940471	17.71483437349976
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	898.0
1	735.0
2	422.0
3	283.5
4	346.5
5	485.5
6	603.5
7	699.5
8	878.1666666666666
9	1059.5
10	1213.8333333333333
11	1400.3333333333333
12	1577.1666666666667
13	1723.1666666666667
14	1834.3333333333335
15	1966.3333333333335
16	2132.0
17	2248.1666666666665
18	2363.666666666667
19	2498.3333333333335
20	2622.166666666667
21	2799.666666666667
22	2989.5
23	3155.5
24	3303.0
25	3480.166666666667
26	3648.166666666667
27	3808.3333333333335
28	3970.666666666667
29	4162.0
30	4371.833333333333
31	4452.333333333333
32	4465.833333333333
33	4571.666666666666
34	4777.833333333333
35	5011.166666666666
36	5174.5
37	5274.0
38	5403.666666666666
39	5434.166666666666
40	5384.833333333333
41	5321.0
42	5228.333333333333
43	5127.833333333333
44	5025.833333333333
45	4924.333333333333
46	4812.5
47	4672.166666666666
48	4448.333333333333
49	4164.333333333333
50	3905.8333333333335
51	3661.0
52	3404.5
53	3186.6666666666665
54	2964.3333333333335
55	2761.666666666667
56	2595.5
57	2471.8333333333335
58	2332.3333333333335
59	2135.166666666667
60	1927.5
61	1731.1666666666665
62	1608.6666666666665
63	1491.8333333333333
64	1341.5
65	1195.8333333333333
66	1047.8333333333333
67	928.8333333333333
68	820.3333333333334
69	688.0
70	597.3333333333333
71	508.0
72	417.83333333333337
73	354.66666666666663
74	309.3333333333333
75	273.5
76	234.0
77	199.5
78	172.0
79	145.5
80	126.0
81	117.5
82	106.0
83	88.5
84	79.0
85	73.5
86	66.0
87	58.5
88	49.5
89	44.0
90	40.5
91	30.0
92	24.0
93	25.0
94	23.0
95	16.5
96	15.5
97	17.5
98	13.0
99	8.5
100	7.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.013649297061201347
3	0.0325483237613263
4	0.025198702266833257
5	0.026248648194617977
6	0.026248648194617977
7	0.0325483237613263
8	0.025198702266833257
9	0.01154940520563191
10-14	0.0029398485977972133
15-19	0.006719653937822202
20-24	0.005669708010037483
25-29	0.00986949172117636
30-34	0.006538527103362437
35-39	0.0018230266496112395
40-44	0.005039541014110715
45-49	0.008634128792421154
50-54	0.026185908565638398
55-59	0.034657595789743915
60-64	0.014861469870139062
65-69	0.02000184632427609
70-74	0.02071062289269412
75-79	0.012319280554015646
80-84	0.011135134131969898
85-89	9.773833492972613E-4
90-94	0.0020567033102639777
95-99	0.0
100-104	0.00287624937082045
105-109	0.008122948955388764
110-114	0.013683400324980758
115-119	0.014426371406931872
120-124	0.003808435685042369
125-129	0.0020073468896159947
130-134	0.0
135-139	0.008967604528640287
140-144	0.011853678196344326
145-149	0.0025184476288815576
150-154	0.01340087373696765
155-159	0.025702535983550375
160-164	0.009140489320861644
165-169	0.006534239414532148
170-174	0.010535927512818712
175-179	0.007504408840193614
180-184	0.0
185-189	0.0
190-194	0.0
195-199	0.0
200	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
30-34	22909.0
35-39	15786.0
40-44	11473.0
45-49	7916.0
50-54	4651.0
55-59	3228.0
60-64	2428.0
65-69	2068.0
70-74	1557.0
75-79	1210.0
80-84	1161.0
85-89	993.0
90-94	1015.0
95-99	1069.0
100-104	5185.0
105-109	682.0
110-114	564.0
115-119	622.0
120-124	567.0
125-129	495.0
130-134	537.0
135-139	495.0
140-144	505.0
145-149	471.0
150-154	476.0
155-159	433.0
160-164	446.0
165-169	460.0
170-174	373.0
175-179	331.0
180-184	331.0
185-189	333.0
190-194	112.0
195-199	195.0
200-201	4166.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	97.29533929002656
#Duplication Level	Percentage of total
1	95.864262990456
2	2.435874552460548
3	0.3149837783354157
4	0.14699242988986067
5	0.09974486313954832
6	0.0440977289669582
7	0.029398485977972132
8	0.04199783711138876
9	0.018899026700124943
>10	0.6226179351763385
>50	0.381130371785853
>100	0.0
>500	0.0
>1k	0.0
>5k	0.0
>10k+	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	0.003149837783354157	0.0	0.0	0.0	0.9218525245949833	0.008399567422277753
2	0.007349621494493034	0.0	0.0	0.0	0.9722499291286498	0.008399567422277753
3	0.01049945927784719	0.0	0.0	0.0	1.0394464685068718	0.012599351133416628
4	0.012599351133416628	0.0	0.0	0.0	1.106643007885094	0.013649297061201347
5	0.012599351133416628	0.0	0.0	0.0	1.1706897094799618	0.017849080772340223
6	0.012599351133416628	0.0	0.0	0.0	1.226336843652552	0.02099891855569438
7	0.013649297061201347	0.0	0.0	0.0	1.2693846266917255	0.02309881041126382
8	0.015749188916770785	0.0	0.0	0.0	1.3271316527198849	0.026248648194617977
9	0.015749188916770785	0.0	0.0	0.0	1.3890784624591834	0.028348540050187415
10-14	0.018479048329011056	0.0	0.0	0.0	1.6574446416009578	0.042417815482502653
15-19	0.02666862656573187	0.0	0.0	0.0	2.3779175372468315	0.06299675566708315
20-24	0.057537036842602605	0.0	0.0	0.0	3.327698623520889	0.07412618250160116
25-29	0.18332055899121197	0.0	0.0	0.0	4.020452946673247	0.08798546874835947
30-34	0.3469021345400711	0.0	0.0	0.0	4.4721396848062325	0.09365517675839694
35-39	0.399819409300421	0.0	0.0	0.0	4.704177734846656	0.09596505779952333
40-44	0.43299770061841814	0.0	0.0	0.0	4.833531073149732	0.10079480906733303
45-49	0.45525655428745415	0.0	0.0	0.0	4.9003076341568415	0.10289470092290247
50-54	0.4798252889976166	0.0	0.0	0.0	4.922146509454763	0.10289470092290247
55-59	0.4980943481410708	0.0	0.0	0.0	4.9355858173304075	0.10289470092290247
60-64	0.5144735046145124	0.0	0.0	0.0	4.948815136020495	0.10373465766513024
65-69	0.523293050407904	0.0	0.0	0.0	4.960154552040569	0.10394464685068719
70-74	0.5417720987369151	0.0	0.0	0.0	4.965824260050608	0.10394464685068719
75-79	0.5562613525403441	0.0	0.0	0.0	4.970234032947303	0.10394464685068719
80-84	0.5669708010037484	0.0	0.0	0.0	4.9748537950295555	0.10394464685068719
85-89	0.5785202062093803	0.0	0.0	0.0	4.979683546297365	0.10541457114958579
90-94	0.5854498493327593	0.0	0.0	0.0	4.981993427338492	0.10604453870625663
95-99	0.5921695032705816	0.0	0.0	0.0	4.982413405709606	0.10604453870625663
100-104	0.6058188003317828	0.0	0.0	0.0	4.987243156977415	0.10604453870625663
105-109	0.612748443455162	0.0	0.0	0.0	4.992072908245225	0.10604453870625663
110-114	0.6192581082074273	0.0	0.0	0.0	4.993122854173009	0.10604453870625663
115-119	0.6272376972585911	0.0	0.0	0.0	4.994592778471909	0.10604453870625663
120-124	0.631647470155287	0.0	0.0	0.0	4.996482681141922	0.10604453870625663
125-129	0.6329074052686287	0.0	0.0	0.0	4.996902659513035	0.10604453870625663
130-134	0.6375271673508814	0.0	0.0	0.0	5.000262486481946	0.10604453870625663
135-139	0.6398370483920077	0.0	0.0	0.0	5.000892454038617	0.10604453870625663
140-144	0.6408869943197925	0.0	0.0	0.0	5.000892454038617	0.10604453870625663
145-149	0.6455067564020454	0.0	0.0	0.0	5.001522421595288	0.10604453870625663
150-154	0.6488665833709565	0.0	0.0	0.0	5.002782356708629	0.10604453870625663
155-159	0.6522264103398675	0.0	0.0	0.0	5.002992345894186	0.10604453870625663
160-164	0.6549562697521077	0.0	0.0	0.0	5.002992345894186	0.10604453870625663
165-169	0.6574761399787911	0.0	0.0	0.0	5.002992345894186	0.10604453870625663
170-174	0.6595760318343604	0.0	0.0	0.0	5.003832302636414	0.10604453870625663
175-179	0.6610459561332592	0.0	0.0	0.0	5.005092237749756	0.10604453870625663
180-184	0.6614659345043731	0.0	0.0	0.0	5.005092237749756	0.10604453870625663
185-189	0.6614659345043731	0.0	0.0	0.0	5.006772151234212	0.10604453870625663
>>END_MODULE
