##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	SRR6235608
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1424019
Total Bases	188.1 Mbp
Sequences flagged as poor quality	0
Sequence length	30-200
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	34.3290819855634	34.0	31.0	34.0	31.0	41.0
2	34.646354437686576	34.0	31.0	34.0	31.0	41.0
3	34.78341932235455	34.0	33.0	34.0	31.0	41.0
4	37.46261040056348	37.0	37.0	41.0	35.0	41.0
5	37.48678353308488	37.0	37.0	41.0	35.0	41.0
6	37.527043529615824	37.0	37.0	41.0	35.0	41.0
7	37.55003971154879	37.0	37.0	41.0	35.0	41.0
8	37.59184112009741	37.0	37.0	41.0	35.0	41.0
9	38.83690807496248	39.0	39.0	41.0	37.0	41.0
10-14	39.096547307304185	39.4	39.2	41.0	37.2	41.0
15-19	39.89124288369747	41.0	40.0	41.0	38.0	41.0
20-24	39.92219836954423	41.0	40.0	41.0	38.0	41.0
25-29	39.88505925833855	41.0	40.0	41.0	38.0	41.0
30-34	39.851044261799	41.0	40.0	41.0	38.0	41.0
35-39	39.7961408637707	41.0	40.0	41.0	37.6	41.0
40-44	39.83761997509568	41.0	40.8	41.0	37.8	41.0
45-49	39.8934771621267	41.0	41.0	41.0	37.2	41.0
50-54	39.889213748280824	41.0	41.0	41.0	37.0	41.0
55-59	39.81236005454904	41.0	41.0	41.0	36.2	41.0
60-64	39.72866063365351	41.0	41.0	41.0	35.4	41.0
65-69	39.65087796454455	41.0	41.0	41.0	35.0	41.0
70-74	39.58647770387504	41.0	41.0	41.0	35.0	41.0
75-79	39.477431978817236	41.0	41.0	41.0	34.6	41.0
80-84	39.65831392892143	41.0	41.0	41.0	35.0	41.0
85-89	39.64135968508319	41.0	41.0	41.0	35.0	41.0
90-94	39.534096367774644	41.0	41.0	41.0	35.0	41.0
95-99	39.31871140413432	41.0	41.0	41.0	34.8	41.0
100-104	36.71064052735365	37.8	35.6	40.2	31.6	41.0
105-109	36.66203540625314	37.4	35.0	40.0	31.8	41.0
110-114	37.007072003074356	39.0	35.0	40.2	32.0	41.0
115-119	37.27340794765754	39.0	35.8	41.0	32.0	41.0
120-124	37.317316133799224	39.0	36.0	41.0	32.0	41.0
125-129	37.29346468984321	39.0	36.0	41.0	31.0	41.0
130-134	37.24657676558631	39.0	36.0	41.0	31.0	41.0
135-139	37.17577745619003	39.0	36.0	41.0	31.0	41.0
140-144	37.24606556563074	39.0	36.0	41.0	31.0	41.0
145-149	37.118299877831554	39.0	35.0	41.0	31.0	41.0
150-154	36.689121056367426	38.2	34.8	40.2	30.8	41.0
155-159	36.73923455867129	38.0	35.0	40.4	31.0	41.0
160-164	36.33344227101345	37.2	35.0	40.0	31.0	41.0
165-169	35.777581057767605	36.8	34.0	39.2	31.0	41.0
170-174	35.15817306640389	35.4	34.0	38.4	30.0	40.2
175-179	34.49168671258527	35.0	34.0	37.0	30.0	39.0
180-184	33.862302865592255	35.0	33.6	35.6	29.6	37.2
185-189	33.27594945324878	35.0	33.0	35.0	28.8	36.2
190-194	33.29624438266381	35.0	33.0	35.0	29.0	35.8
195-199	33.33016805286444	35.0	33.0	35.0	29.4	35.0
200	33.478062639182674	35.0	33.0	35.0	30.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	1.0
13	0.0
14	3.0
15	3.0
16	6.0
17	13.0
18	59.0
19	172.0
20	205.0
21	213.0
22	310.0
23	490.0
24	954.0
25	1503.0
26	2418.0
27	3438.0
28	4545.0
29	6070.0
30	7556.0
31	9940.0
32	12895.0
33	17228.0
34	24234.0
35	36181.0
36	60352.0
37	102972.0
38	149578.0
39	323985.0
40	385807.0
41	272888.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	18.319067371994336	32.10582162176207	23.673981878050785	25.901129128192814
2	19.774075972835746	31.78021391500987	21.62381577746097	26.821894334693418
3	20.63573991755676	30.85455860562777	21.448655557194122	27.06104591962135
4	22.008115157141635	29.018006997181185	21.151725910497316	27.822151935179868
5	24.01962333367743	27.940006418453688	20.130279160601088	27.910091087267798
6	24.305967151593123	29.240601799443255	22.00237922016635	24.451051828797272
7	22.700610033995332	26.13420186107067	26.94584833488879	24.219339770045202
8	23.182626074511646	28.048221266710627	30.370311070287688	18.398841588490043
9	23.73528723984722	29.747917689300497	29.595883200996614	16.920911869855672
10-14	24.215688133374623	29.023306571049968	27.291363387707605	19.469641907867803
15-19	23.345127674811565	28.752664471092395	26.878668936491028	21.023538917605013
20-24	22.65019944289975	28.512588968415116	27.012241289883836	21.82497029880129
25-29	22.692827028634976	28.487518458479272	26.682723706094663	22.136930806791092
30-34	22.632899262940715	28.38253247655182	27.07947778840601	21.90509047210146
35-39	22.602461548084317	28.701517678069337	26.91677064343222	21.779250130414123
40-44	22.665548280742925	28.418282361468393	26.941467778424	21.974701579364677
45-49	22.455886955670387	28.564745014510827	27.089490499420997	21.889877530397786
50-54	22.295811284999868	28.766028506855918	27.066483070115833	21.871677138028385
55-59	22.375051812396197	28.66329987963035	27.106525717996142	21.85512258997731
60-64	22.156934493638445	28.83799106990307	27.276847242946477	21.728227193512012
65-69	21.976843837404758	28.806824860123857	27.322120799579146	21.89421050289224
70-74	22.024591224322197	28.75324937085319	27.16859276243516	22.05356664238945
75-79	21.848395525713222	28.85123830302155	27.565355080680032	21.735011090585196
80-84	22.04510361244797	28.51996250030158	26.96380831036515	22.471125576885296
85-89	22.31510775200588	28.344502028300262	26.529901817644113	22.810488402049742
90-94	22.246584603088777	28.223106282823785	26.704570481242047	22.82573863284539
95-99	22.161033504653357	28.187620981974863	26.65409351720135	22.997251996170426
100-104	23.982284678170664	26.26548737524741	25.704696195168825	24.0475317514131
105-109	22.73053909557635	27.680217917320054	26.629568109319067	22.95967487778453
110-114	22.061290345197488	28.144795745590816	27.126109087594696	22.667804821617
115-119	22.225457185277268	27.89453307471719	26.795526205714758	23.084483534290783
120-124	22.100650191370246	27.792411475176493	26.85843434086121	23.248503992592052
125-129	22.168661813567578	27.744233873706698	26.763873171958398	23.323231140767334
130-134	22.108775293102543	27.751655106307982	26.785561061500896	23.354008539088582
135-139	21.90291364907421	27.85342660303626	26.928362869719397	23.315296878170123
140-144	22.048446501381548	27.82896651427391	26.72235886459575	23.400228119748785
145-149	22.025202884130113	27.789594301291352	26.806048031451223	23.37915478312731
150-154	21.860849579060496	27.649481165339314	26.946125741106002	23.543543514494193
155-159	21.945867019399405	27.424521211108928	26.9081377274062	23.721474042085468
160-164	22.095446616048754	27.534433246200194	27.015849560149263	23.354270577601785
165-169	21.907112168687114	27.61317324212975	27.0112484567974	23.468466132385736
170-174	22.06822072287487	27.480782656304953	26.949090889920978	23.5019057308992
175-179	22.15285234828674	27.520834122903427	26.953438525125172	23.37287500368466
180-184	22.024690144266902	27.372614340097805	26.974062964689065	23.62863255094623
185-189	22.04793492264611	27.35165408490295	27.394882824561588	23.20552816788935
190-194	21.5303927621223	28.04167964908664	28.656843656142893	21.771083932648168
195-199	21.144617508240803	28.325227333455555	28.538271942216742	21.991883216086904
200	21.417538723024148	28.347052583912806	28.64046052019428	21.59494817286876
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	1.0
2	2.0
3	2.0
4	3.0
5	7.0
6	9.5
7	16.0
8	23.5
9	32.5
10	50.16666666666667
11	71.99999999999999
12	104.16666666666666
13	143.83333333333337
14	180.50000000000006
15	236.16666666666677
16	359.16666666666634
17	507.49999999999943
18	720.1666666666686
19	988.8333333333368
20	1330.1666666666576
21	1772.6666666666538
22	2443.8333333333726
23	3369.666666666734
24	4438.833333333144
25	5827.166666666409
26	7542.499999999647
27	9901.50000000103
28	12655.833333334685
29	15362.666666668489
30	18258.499999995463
31	21600.833333327784
32	25085.833333326947
33	28898.33333332609
34	32676.333333324812
35	36491.00000001909
36	40305.50000002102
37	43090.00000002221
38	45167.50000002276
39	47065.16666669014
40	48554.16666669021
41	49649.000000023865
42	50487.6666666908
43	51048.16666669059
44	51379.666666689925
45	51290.33333335596
46	51418.33333335586
47	51733.16666668857
48	51796.166666688216
49	52181.83333335432
50	52213.83333335349
51	51170.00000001949
52	49227.16666668498
53	46382.33333335021
54	42181.833333347924
55	37304.500000012624
56	32724.666666661305
57	28407.999999995514
58	24604.333333329603
59	21187.666666663692
60	18148.999999997413
61	15776.833333334424
62	13798.000000000853
63	11887.833333334022
64	9779.333333333854
65	7584.333333333133
66	5625.499999999829
67	4212.499999999999
68	3247.5000000000427
69	2460.5000000000264
70	1911.8333333333233
71	1472.666666666659
72	1049.5
73	734.5000000000015
74	559.5000000000008
75	409.9999999999998
76	254.33333333333337
77	148.1666666666667
78	91.16666666666666
79	53.5
80	33.5
81	17.0
82	7.0
83	7.0
84	6.5
85	3.5
86	2.0
87	2.0
88	2.0
89	1.0
90	0.5
91	1.0
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0062499166092587245
3	6.320140391385228E-4
4	0.001193804296150543
5	0.0
6	0.0016151469889095582
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	1.9662658995420708E-4
20-24	1.6853707710360607E-4
25-29	8.567301419433309E-4
30-34	4.217549645831269E-5
35-39	2.394852588370765E-4
40-44	0.0016241660084086605
45-49	8.076068060000935E-4
50-54	4.5416380212310226E-4
55-59	0.0
60-64	0.0
65-69	0.0012552463592149917
70-74	0.0012579204316725495
75-79	5.730795316908683E-4
80-84	1.4361095676922453E-5
85-89	7.460546392512476E-5
90-94	1.595902743133389E-4
95-99	0.0
100-104	7.229026878448733E-4
105-109	5.631322542244472E-4
110-114	7.230269853389845E-4
115-119	5.028001177988848E-4
120-124	4.4644979186248083E-4
125-129	9.827501337551837E-4
130-134	8.931353932650573E-4
135-139	4.587013529572829E-4
140-144	5.08494796729205E-4
145-149	0.0011736691244167517
150-154	0.00120916469451905
155-159	1.616389761140697E-4
160-164	4.2151306329199294E-4
165-169	6.06164286686073E-4
170-174	8.284080708032848E-4
175-179	0.001010639507414304
180-184	2.8359837176721486E-4
185-189	0.0013841523078610269
190-194	0.0019690420263064013
195-199	3.5946655163737013E-4
200	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
30-34	3360.0
35-39	2701.0
40-44	5051.0
45-49	2809.0
50-54	2224.0
55-59	2215.0
60-64	2473.0
65-69	2784.0
70-74	3126.0
75-79	3274.0
80-84	19549.0
85-89	86383.0
90-94	87362.0
95-99	86403.0
100-104	86715.0
105-109	82475.0
110-114	79324.0
115-119	75539.0
120-124	71063.0
125-129	67084.0
130-134	61988.0
135-139	57238.0
140-144	52995.0
145-149	48497.0
150-154	43504.0
155-159	40731.0
160-164	36755.0
165-169	32976.0
170-174	29117.0
175-179	26794.0
180-184	24277.0
185-189	23076.0
190-194	4252.0
195-199	8696.0
200-201	161209.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	83.16159721657279
#Duplication Level	Percentage of total
1	71.53882789817517
2	19.271087055459606
3	4.046937844897937
4	1.1073799484850775
5	0.5439299048303603
6	0.3247317664911362
7	0.2867592863382563
8	0.2007169634281248
9	0.18179985758236514
>10	1.8667134798838172
>50	0.2957150466861773
>100	0.28527059797437626
>500	0.05013034976761777
>1k	0.0
>5k	0.0
>10k+	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	0.026333918297438447	0.0	0.0	0.0	2.1067134637950757E-4	0.0
2	0.03637591914152831	0.0	0.0	0.0	4.915664748855177E-4	0.0
3	0.04873530479579275	0.0	0.0	0.0	7.022378212650253E-4	0.0
4	0.05870708185775611	0.0	0.0	7.022378212650253E-5	9.129091676445329E-4	0.0
5	0.08258316778076696	0.0	0.0	7.022378212650253E-5	9.831329497710353E-4	0.0
6	0.12289161872137942	0.0	0.0	7.022378212650253E-5	0.0014044756425300505	0.0
7	0.19416875757977947	0.0	0.0	7.022378212650253E-5	0.0019662658995420706	0.0
8	0.24402764288959627	0.0	0.0	7.022378212650253E-5	0.003440965324198624	0.0
9	0.2563870285438607	0.0	0.0	7.022378212650253E-5	0.0042134269275901515	0.0
10-14	0.2658953286437892	8.426853855180303E-5	0.0	7.022378212650253E-5	0.006769572596994844	0.0
15-19	0.27360589992127915	2.808951285060101E-4	1.4044756425300506E-5	7.022378212650253E-5	0.011039178550286197	2.808951285060101E-5
20-24	0.287763014397982	5.337007441614191E-4	1.2640280782770454E-4	7.022378212650253E-5	0.016994155274613613	1.5449232067830558E-4
25-29	0.31225706960370614	0.0013342518604035482	1.4044756425300506E-4	8.426853855180303E-5	0.022963176755366323	3.0898464135661115E-4
30-34	0.3327905034974955	0.0017134602838866618	1.4044756425300506E-4	1.4044756425300506E-4	0.02749963308073839	3.5111891063251263E-4
35-39	0.34437742754836836	0.0018117735788637651	1.4044756425300506E-4	1.4044756425300506E-4	0.03144620963624783	5.056112313108182E-4
40-44	0.3580710650630364	0.001896042117415568	1.6853707710360607E-4	1.4044756425300506E-4	0.0361933373079994	6.741483084144243E-4
45-49	0.3763432931723524	0.001896042117415568	2.1067134637950757E-4	1.4044756425300506E-4	0.04039271947916425	7.022378212650253E-4
50-54	0.38653978633712055	0.0019662658995420706	2.1067134637950757E-4	1.4044756425300506E-4	0.04419884847042069	7.303273341156263E-4
55-59	0.3948261926280478	0.002120758220220376	2.1067134637950757E-4	1.4044756425300506E-4	0.04786452989742412	8.145958726674294E-4
60-64	0.40207328694350286	0.002387608592301086	2.6685037208070957E-4	1.4044756425300506E-4	0.051670658888680554	8.426853855180303E-4
65-69	0.4087866805147965	0.0026263694515311943	2.808951285060101E-4	1.4044756425300506E-4	0.05755541183088146	9.550434369204344E-4
70-74	0.41697477351074663	0.002668503720807096	2.808951285060101E-4	1.4044756425300506E-4	0.0636227466066113	9.831329497710353E-4
75-79	0.42549994066090413	0.002668503720807096	2.808951285060101E-4	1.4044756425300506E-4	0.06749909937999424	9.831329497710353E-4
80-84	0.4339127497596591	0.002696593233657697	2.808951285060101E-4	1.4044756425300506E-4	0.07138949690980247	9.831329497710353E-4
85-89	0.44545753954125616	0.0027387275029335987	2.808951285060101E-4	1.4044756425300506E-4	0.07454956710549508	9.831329497710353E-4
90-94	0.45297148422879185	0.002808951285060101	2.808951285060101E-4	1.4044756425300506E-4	0.07630516165865765	9.831329497710353E-4
95-99	0.4561175096680592	0.002808951285060101	2.808951285060101E-4	1.4044756425300506E-4	0.07763941351906119	9.831329497710353E-4
100-104	0.4591511770559241	0.002808951285060101	2.808951285060101E-4	1.4044756425300506E-4	0.07901579964874064	9.831329497710353E-4
105-109	0.46252191859799624	0.002808951285060101	3.0898464135661115E-4	1.4044756425300506E-4	0.0800972458934888	9.831329497710353E-4
110-114	0.46519042231880336	0.002808951285060101	3.5111891063251263E-4	1.4044756425300506E-4	0.08098206554828272	9.97177706196336E-4
115-119	0.46760612042395505	0.002837040797910702	3.5111891063251263E-4	1.4044756425300506E-4	0.08193710898520315	0.0010533567318975379
120-124	0.4695864310799224	0.0028791750671866034	3.5111891063251263E-4	1.4044756425300506E-4	0.08256912302434168	0.0010533567318975379
125-129	0.4710049514788778	0.0028791750671866034	3.5111891063251263E-4	1.4044756425300506E-4	0.08331349511488259	0.0011235805140240405
130-134	0.4729852621348451	0.002893219823611904	3.5111891063251263E-4	1.5449232067830558E-4	0.08411404623112473	0.001137625270449341
135-139	0.4749093937651113	0.002949398849313106	3.5111891063251263E-4	2.1067134637950757E-4	0.08498482112949336	0.001193804296150543
140-144	0.47684757015180274	0.002949398849313106	3.5111891063251263E-4	2.1067134637950757E-4	0.08584155127143668	0.001193804296150543
145-149	0.47864529897424124	0.002949398849313106	3.5111891063251263E-4	2.1067134637950757E-4	0.08641738628487401	0.001193804296150543
150-154	0.47985314802681706	0.002977488362163707	3.5111891063251263E-4	2.1067134637950757E-4	0.08695108702903544	0.0012359385654264446
155-159	0.4811031313486688	0.0030196226314396085	3.5111891063251263E-4	2.1067134637950757E-4	0.08738647447821975	0.0012640280782770454
160-164	0.48221266710626753	0.0030196226314396085	3.5111891063251263E-4	2.1067134637950757E-4	0.08770950387600165	0.0012640280782770454
165-169	0.48306939724821085	0.0030196226314396085	3.5111891063251263E-4	2.1067134637950757E-4	0.08828533888943897	0.0012640280782770454
170-174	0.48375759031305055	0.0030196226314396085	3.5111891063251263E-4	2.949398849313106E-4	0.08845387596654258	0.0012640280782770454
175-179	0.4842491567879361	0.0030196226314396085	3.5111891063251263E-4	3.5111891063251263E-4	0.08859432353079558	0.0012640280782770454
180-184	0.4846985889935458	0.0030196226314396085	3.5111891063251263E-4	3.5111891063251263E-4	0.08862241304364618	0.0012640280782770454
185-189	0.48483903655779875	0.0030196226314396085	3.5111891063251263E-4	3.5111891063251263E-4	0.08863645780007148	0.0012640280782770454
>>END_MODULE
