FastQCFastQC Report
Wed 13 Sep 2023
stdin:SRR6235610

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6235610
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5442980
Total Bases511.1 Mbp
Sequences flagged as poor quality0
Sequence length30-200
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTGAGATCTCGTATG587821.0799598749214585TruSeq Adapter, Index 13 (97% over 38bp)
CAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTGAGATCTCGTAT115600.21238365748174715TruSeq Adapter, Index 13 (97% over 38bp)
CCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTGAGATCTCGTA63060.11585565260206726TruSeq Adapter, Index 13 (97% over 38bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)