FastQCFastQC Report
Wed 13 Sep 2023
stdin:SRR6235611

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6235611
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2046656
Total Bases143 Mbp
Sequences flagged as poor quality0
Sequence length30-200
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGC122838460.01907501798055TruSeq Adapter, Index 7 (100% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTC463112.2627642359048123TruSeq Adapter, Index 7 (100% over 44bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCA239621.1707878607836393TruSeq Adapter, Index 7 (100% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCG228411.116015588354858TruSeq Adapter, Index 7 (100% over 45bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTAT188720.9220894962319022TruSeq Adapter, Index 7 (100% over 48bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATC164870.8055579442759311TruSeq Adapter, Index 7 (100% over 42bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTA158100.772479595984865TruSeq Adapter, Index 7 (100% over 47bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCT133450.6520392288689453TruSeq Adapter, Index 7 (100% over 43bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGT130050.6354267644391632TruSeq Adapter, Index 7 (100% over 46bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATG101060.49378107508052155TruSeq Adapter, Index 7 (100% over 49bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCAT95780.46798289502486007TruSeq Adapter, Index 7 (100% over 41bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATC87450.42728235717189406TruSeq Adapter, Index 7 (100% over 39bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATATCGTATGC80340.3925427624378498TruSeq Adapter, Index 7 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGGATGC73240.357852027893305TruSeq Adapter, Index 7 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCAGCTCGTATGC55140.26941508489946525TruSeq Adapter, Index 7 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCACCTCGTATGC54830.2679004190249851TruSeq Adapter, Index 7 (98% over 50bp)
GATCGGAAGAGTACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGC52530.2566625754401326TruSeq Adapter, Index 7 (98% over 50bp)
GATCGGAAGAGCACACGTTTGAACTCCAGTCACCAGATCATCTCGTATGC47220.23071781481597298TruSeq Adapter, Index 7 (98% over 50bp)
GATCGGAAGAGCACATGTCTGAACTCCAGTCACCAGATCATCTCGTATGC46400.22671127927702556TruSeq Adapter, Index 7 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCAACTCGTATGC45930.2244148503705557TruSeq Adapter, Index 7 (98% over 50bp)
GAATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATG44320.2165483598611589TruSeq Adapter, Index 7 (97% over 49bp)
GATCGGAAGAGCACACGTCTGAATTCCAGTCACCAGATCATCTCGTATGC43650.2132747271647018TruSeq Adapter, Index 7 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCTAGTCACCAGATCATCTCGTATGC43430.21219980299571595TruSeq Adapter, Index 7 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGCATGC43430.21219980299571595TruSeq Adapter, Index 7 (98% over 50bp)
TCTGCAGGATATCGCGGCCGCCATCTGCCCTACGTTTGAGGGTTATAAGC41590.2032095281278339No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGAT40880.1997404546733794TruSeq Adapter, Index 7 (100% over 38bp)
GATCGGAAGAGCACACGTCTGAACTTCAGTCACCAGATCATCTCGTATGC40300.19690656368241657TruSeq Adapter, Index 7 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATTC38680.18899121298352042TruSeq Adapter, Index 7 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGA38110.18620618218205698TruSeq Adapter, Index 7 (100% over 37bp)
GATTGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGC37710.1842517746020826TruSeq Adapter, Index 7 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGC36440.17804653053566402TruSeq Adapter, Index 6 (100% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTTACCAGATCATCTCGTATGC35600.17394227461771788TruSeq Adapter, Index 7 (98% over 50bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGCC33700.16465883861283967TruSeq Adapter, Index 7 (100% over 50bp)
TCTGCAGGATATCGCGGCCGCGTCTTCAGAGGGGGATAGCATGACCTCAC33230.16236240970636978No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCACATCATCTCGTATGC32970.1610920447793865TruSeq Adapter, Index 7 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCA32050.15659690734544546TruSeq Adapter, Index 7 (100% over 35bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGATCATCTCGTATGC31300.15293239313299353TruSeq Adapter, Index 7 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACC31220.15254151161699867TruSeq Adapter, Index 2 (100% over 34bp)
GATCGGAAGAGCACACGTCTGAACTCCCAGTCACCAGATCATCTCGTATG29950.14633626755058007Illumina Multiplexing PCR Primer 2.01 (96% over 27bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAG28880.14110822727414868TruSeq Adapter, Index 7 (100% over 36bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATG28290.1382254760936865TruSeq Adapter, Index 7 (100% over 49bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCATGCCAATATCTCGTATGC27140.1326065543012602TruSeq Adapter, Index 6 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGAACATCTCGTATGC26220.1281114168673192TruSeq Adapter, Index 7 (98% over 50bp)
GATCGGAAGAGCATACGTCTGAACTCCAGTCACCAGATCATCTCGTATGC25240.12332311829638201TruSeq Adapter, Index 7 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCCAATATCTCGTATGC23000.11237843584852558TruSeq Adapter, Index 6 (98% over 50bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)