FastQCFastQC Report
Wed 13 Sep 2023
stdin:SRR6235612

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6235612
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4143392
Total Bases289.8 Mbp
Sequences flagged as poor quality0
Sequence length30-200
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGC295169271.23854078976838TruSeq Adapter, Index 7 (100% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTC596131.4387487353356865TruSeq Adapter, Index 7 (100% over 44bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCG364280.8791830461612129TruSeq Adapter, Index 7 (100% over 45bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCA346210.8355714351912636TruSeq Adapter, Index 7 (100% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTAT307230.741493925749724TruSeq Adapter, Index 7 (100% over 48bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTA226240.5460260578772175TruSeq Adapter, Index 7 (100% over 47bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGT199910.48247908959615693TruSeq Adapter, Index 7 (100% over 46bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATG189820.45812706111321355TruSeq Adapter, Index 7 (100% over 49bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATC186070.4490765054332295TruSeq Adapter, Index 7 (100% over 42bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATATCGTATGC160920.38837744533947066TruSeq Adapter, Index 7 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATC142760.3445486210332018TruSeq Adapter, Index 7 (100% over 39bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGGATGC142540.34401765509997606TruSeq Adapter, Index 7 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCT141610.34177311729134TruSeq Adapter, Index 7 (100% over 43bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCACCTCGTATGC132430.3196173569867394TruSeq Adapter, Index 7 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCAGCTCGTATGC125230.3022402900811702TruSeq Adapter, Index 7 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCAT116380.2808809786764081TruSeq Adapter, Index 7 (100% over 41bp)
GATCGGAAGAGTACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGC116210.2804706868189155TruSeq Adapter, Index 7 (98% over 50bp)
GATCGGAAGAGCACATGTCTGAACTCCAGTCACCAGATCATCTCGTATGC106570.25720472501757013TruSeq Adapter, Index 7 (98% over 50bp)
GATCGGAAGAGCACACGTTTGAACTCCAGTCACCAGATCATCTCGTATGC106320.25660135463890454TruSeq Adapter, Index 7 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCTAGTCACCAGATCATCTCGTATGC102390.24711637228628142TruSeq Adapter, Index 7 (98% over 50bp)
GAATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATG100640.24289277963562222TruSeq Adapter, Index 7 (97% over 49bp)
GATCGGAAGAGCACACGTCTGAATTCCAGTCACCAGATCATCTCGTATGC99660.2405275677512531TruSeq Adapter, Index 7 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGCATGC96060.2318390342984685TruSeq Adapter, Index 7 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTTCAGTCACCAGATCATCTCGTATGC93200.2249364771665341TruSeq Adapter, Index 7 (98% over 50bp)
GATTGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGC88160.21277253033263566TruSeq Adapter, Index 7 (98% over 50bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGCC83710.2020325375923881TruSeq Adapter, Index 7 (100% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCAACTCGTATGC83310.20106714498652314TruSeq Adapter, Index 7 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTTACCAGATCATCTCGTATGC74740.1803836084058665TruSeq Adapter, Index 7 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCCAGTCACCAGATCATCTCGTATG66870.16138950888547354Illumina Multiplexing PCR Primer 2.01 (96% over 27bp)
GATCGGAAGAGCACACGTCTGAACTCCAGGCACCAGATCATCTCGTATGC66730.1610516214734208TruSeq Adapter, Index 7 (98% over 50bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATG63500.15325607618106132TruSeq Adapter, Index 7 (100% over 49bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGA62610.1511080776330118TruSeq Adapter, Index 7 (100% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGAT61300.14794641684880408TruSeq Adapter, Index 7 (100% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATTC60940.1470775635035256TruSeq Adapter, Index 7 (98% over 50bp)
GATCGGAAGAGCATACGTCTGAACTCCAGTCACCAGATCATCTCGTATGC54260.1309555069855809TruSeq Adapter, Index 7 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACC54250.13093137217043427TruSeq Adapter, Index 2 (100% over 34bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCA53630.12943501363134358TruSeq Adapter, Index 7 (100% over 35bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAG52040.1255975780230304TruSeq Adapter, Index 7 (100% over 36bp)
TCTGCAGGATATCGCGGCCGCCATCTGCCCTACGTTTGAGGGTTATAAGC50690.12233937797823619No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGATCATCTCGTATGC48680.1174882801337648TruSeq Adapter, Index 7 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCACATCATCTCGTATGC45510.10983754373228505TruSeq Adapter, Index 7 (98% over 50bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)