FastQCFastQC Report
Wed 13 Sep 2023
stdin:SRR6235615

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6235615
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2205952
Total Bases159.4 Mbp
Sequences flagged as poor quality0
Sequence length30-200
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGC155631170.55053781768596TruSeq Adapter, Index 5 (100% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATATCGTATGC302891.3730579813160033TruSeq Adapter, Index 5 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGCGATCTCGTATGC135530.6143832685389347TruSeq Adapter, Index 5 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGAGCTCGTATGC106840.4843260415457816TruSeq Adapter, Index 5 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGGGATCTCGTATGC103980.471361117558315TruSeq Adapter, Index 5 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGAGATCTCGTATGC74210.33640804514332134TruSeq Adapter, Index 5 (98% over 50bp)
GATCGGAAGAGCACATGTCTGAACTCCAGTCACACAGTGATCTCGTATGC70340.3188645990483927TruSeq Adapter, Index 5 (98% over 50bp)
GATTGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGC68780.31179282232795635TruSeq Adapter, Index 5 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGAACTCGTATGC65970.2990545578507601TruSeq Adapter, Index 5 (98% over 50bp)
GATCGGAAGAGCACACGTTTGAACTCCAGTCACACAGTGATCTCGTATGC65060.2949293547638389TruSeq Adapter, Index 5 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGACCTCGTATGC60450.2740313479169085TruSeq Adapter, Index 5 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTAT59040.2676395497272833TruSeq Adapter, Index 5 (100% over 48bp)
GATCGGAAGAGCACACGTCTGAATTCCAGTCACACAGTGATCTCGTATGC58300.2642849889752814TruSeq Adapter, Index 5 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTTACACAGTGATCTCGTATGC57640.2612930834397122TruSeq Adapter, Index 5 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCTAGTCACACAGTGATCTCGTATGC54200.24569890913310896TruSeq Adapter, Index 5 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAG50680.2297420796100731TruSeq Adapter, Index 5 (100% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATG49210.2230782900081235TruSeq Adapter, Index 5 (100% over 49bp)
GATCGGAAGAGCACACGTCTGAACTTCAGTCACACAGTGATCTCGTATGC45920.20816409423233145TruSeq Adapter, Index 5 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTA45840.20780143901589881TruSeq Adapter, Index 5 (100% over 47bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGA45480.20616949054195194TruSeq Adapter, Index 5 (100% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATGTCGTATGC41700.18903403156551005TruSeq Adapter, Index 5 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCG40660.1843195137518858TruSeq Adapter, Index 5 (100% over 45bp)
GATCGGAAGAGTACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGC40090.1817355953348033TruSeq Adapter, Index 5 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATTTCGTATGC35160.15938696762214227TruSeq Adapter, Index 5 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCCAGTCACACAGTGATCTCGTATG34940.15838966577695252Illumina Multiplexing PCR Primer 2.01 (96% over 27bp)
GATCGGAAGAGCACACGTCTGAACTCCAGGCACACAGTGATCTCGTATGC33800.15322182894278752TruSeq Adapter, Index 5 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACA33750.15299516943251712TruSeq Adapter, Index 5 (100% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTC32390.14683003075316234TruSeq Adapter, Index 5 (100% over 44bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGGATGC30670.13903294359986074TruSeq Adapter, Index 5 (98% over 50bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATG28080.12729198096785424TruSeq Adapter, Index 5 (100% over 49bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGT27140.1230307821747708TruSeq Adapter, Index 5 (100% over 46bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTG26120.11840692816525472TruSeq Adapter, Index 5 (100% over 39bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGCATGC25460.1154150226296855TruSeq Adapter, Index 5 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGCCACACAGTGATCTCGTATGC23080.10462602994081467TruSeq Adapter, Index 5 (98% over 50bp)
TCTGCAGGATATCGCGGCCGCCATCTGCCCTACGTTTGAGGGTTATAAGC22990.10421804282232795No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAC22370.10140746489497506TruSeq Adapter, Index 5 (100% over 35bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)