FastQCFastQC Report
Wed 13 Sep 2023
stdin:SRR6235616

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6235616
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3841926
Total Bases267.3 Mbp
Sequences flagged as poor quality0
Sequence length30-200
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGC307899280.1418871680506TruSeq Adapter, Index 5 (100% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATATCGTATGC363770.9468428074877028TruSeq Adapter, Index 5 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGGGATCTCGTATGC238020.6195330154719274TruSeq Adapter, Index 5 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGAGCTCGTATGC178530.46468880452148215TruSeq Adapter, Index 5 (98% over 50bp)
GATCGGAAGAGCACATGTCTGAACTCCAGTCACACAGTGATCTCGTATGC125150.3257480753143085TruSeq Adapter, Index 5 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGACCTCGTATGC123940.32259861329968353TruSeq Adapter, Index 5 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGCGATCTCGTATGC121610.31653394677565366TruSeq Adapter, Index 5 (98% over 50bp)
GATTGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGC115080.29953726334135533TruSeq Adapter, Index 5 (98% over 50bp)
GATCGGAAGAGCACACGTTTGAACTCCAGTCACACAGTGATCTCGTATGC109490.28498726940602187TruSeq Adapter, Index 5 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGAGATCTCGTATGC96540.2512802172660275TruSeq Adapter, Index 5 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAATTCCAGTCACACAGTGATCTCGTATGC94160.24508540768354206TruSeq Adapter, Index 5 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCTAGTCACACAGTGATCTCGTATGC92320.24029614313237682TruSeq Adapter, Index 5 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTTACACAGTGATCTCGTATGC91150.23725079556451636TruSeq Adapter, Index 5 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAG83170.2164799634350063TruSeq Adapter, Index 5 (100% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTAT80440.2093741524433318TruSeq Adapter, Index 5 (100% over 48bp)
GATCGGAAGAGCACACGTCTGAACTTCAGTCACACAGTGATCTCGTATGC79550.20705760600282255TruSeq Adapter, Index 5 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGAACTCGTATGC77390.20143542587754162TruSeq Adapter, Index 5 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATG73110.19029518007374427TruSeq Adapter, Index 5 (100% over 49bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATGTCGTATGC69470.1808207654181783TruSeq Adapter, Index 5 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGA69420.1806906223597227TruSeq Adapter, Index 5 (100% over 40bp)
GATCGGAAGAGTACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGC67380.17538078557473516TruSeq Adapter, Index 5 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTA63360.1649172836749068TruSeq Adapter, Index 5 (100% over 47bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCG60310.15697855710911662TruSeq Adapter, Index 5 (100% over 45bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATTTCGTATGC58380.15195503505273136TruSeq Adapter, Index 5 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCCAGTCACACAGTGATCTCGTATG58160.15138240559552682Illumina Multiplexing PCR Primer 2.01 (96% over 27bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACA53560.13940924421761378TruSeq Adapter, Index 5 (100% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGGATGC51530.13412543604431737TruSeq Adapter, Index 5 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTC48750.1268894819941873TruSeq Adapter, Index 5 (100% over 44bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGCC47840.12452087833029579TruSeq Adapter, Index 5 (100% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGGCACACAGTGATCTCGTATGC46690.12152758798581753TruSeq Adapter, Index 5 (98% over 50bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATG45700.11895075542839711TruSeq Adapter, Index 5 (100% over 49bp)
GGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGCCGTC40250.10476516205673925TruSeq Adapter, Index 5 (100% over 50bp)
GATCGGAAGAGCACACGTCTGACTCCAGTCACACAGTGATCTCGTATGCC40110.10440076149306364TruSeq Adapter, Index 5 (96% over 30bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTG40070.10429664704629917TruSeq Adapter, Index 5 (100% over 39bp)
GATCGGAAGAGCACACGTCTGAACTCAGTCACACAGTGATCTCGTATGCC40050.10424458982291693Illumina Multiplexing PCR Primer 2.01 (96% over 26bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGT39350.10242258700453888TruSeq Adapter, Index 5 (100% over 46bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)