##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	SRR6235617
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	191376
Total Bases	11.7 Mbp
Sequences flagged as poor quality	0
Sequence length	30-200
%GC	37
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	26.993651241534987	28.0	16.0	34.0	10.0	41.0
2	26.664179416436752	30.0	16.0	34.0	10.0	41.0
3	26.561522865981104	30.0	16.0	34.0	10.0	41.0
4	28.590131468940726	33.0	19.0	37.0	10.0	41.0
5	28.45261161274141	33.0	19.0	37.0	10.0	41.0
6	28.212126912465514	33.0	17.0	37.0	10.0	41.0
7	28.209043976256165	33.0	17.0	37.0	10.0	41.0
8	28.11350430566006	32.0	17.0	37.0	10.0	41.0
9	28.628615918401472	33.0	17.0	39.0	10.0	41.0
10-14	28.41142881030014	32.0	17.0	39.4	10.0	41.0
15-19	28.346605634980353	32.0	17.2	41.0	10.0	41.0
20-24	28.41364225399214	32.0	17.4	41.0	10.0	41.0
25-29	28.813226318869663	32.2	17.2	41.0	9.8	41.0
30-34	30.3146808910115	33.8	23.4	41.0	9.4	41.0
35-39	31.836058852505943	36.0	26.0	41.0	10.2	41.0
40-44	33.376199467764295	38.8	30.0	41.0	15.0	41.0
45-49	34.85858791972523	40.4	31.8	41.0	18.4	41.0
50-54	35.87491283012852	41.0	34.0	41.0	23.0	41.0
55-59	36.8560510490919	41.0	36.0	41.0	26.6	41.0
60-64	37.559603394819504	41.0	37.4	41.0	29.8	41.0
65-69	37.788407605047325	41.0	37.2	41.0	30.6	41.0
70-74	37.88098891526296	41.0	37.0	41.0	31.0	41.0
75-79	37.67071077377636	41.0	36.2	41.0	30.8	41.0
80-84	37.694397545422284	41.0	35.6	41.0	31.2	41.0
85-89	37.56931280127087	41.0	35.0	41.0	31.2	41.0
90-94	37.386473728453225	41.0	35.0	41.0	31.0	41.0
95-99	37.14090247429514	41.0	35.0	41.0	31.0	41.0
100-104	35.813218838757166	37.8	33.8	40.2	30.4	41.0
105-109	36.30530622504824	37.4	35.0	40.0	31.0	41.0
110-114	36.79020737589788	39.0	35.2	40.2	31.0	41.0
115-119	37.15322980987536	39.0	36.0	41.0	31.0	41.0
120-124	37.14699367550385	39.0	36.0	41.0	31.0	41.0
125-129	37.07536128664739	39.0	36.0	41.0	31.0	41.0
130-134	37.06656996132147	39.0	36.0	41.0	31.0	41.0
135-139	37.16407931850465	39.0	36.0	41.0	31.0	41.0
140-144	37.05648959340271	39.0	35.8	41.0	31.0	41.0
145-149	36.8941496552862	39.0	35.2	41.0	30.6	41.0
150-154	36.49548753050224	38.6	34.8	40.4	30.0	41.0
155-159	36.673396109980295	39.0	35.0	41.0	30.2	41.0
160-164	36.832896352336526	38.8	35.0	41.0	31.0	41.0
165-169	36.34479029397078	38.0	34.4	40.4	29.8	41.0
170-174	35.864706292001316	37.0	34.0	40.0	29.6	41.0
175-179	35.55579884689613	36.8	34.0	39.0	29.8	41.0
180-184	35.112137126688424	35.8	34.0	38.2	30.0	40.8
185-189	34.555461854526854	35.0	34.0	37.0	29.2	39.6
190-194	34.18085702731404	35.0	34.0	36.2	29.0	39.0
195-199	34.0811373488143	35.0	34.0	36.0	29.6	37.4
200	34.17008639308855	35.0	34.0	36.0	30.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	6.0
10	9.0
11	11.0
12	15.0
13	28.0
14	34.0
15	55.0
16	62.0
17	727.0
18	6566.0
19	17977.0
20	21336.0
21	18287.0
22	13994.0
23	10296.0
24	7526.0
25	5594.0
26	4489.0
27	3743.0
28	2862.0
29	2503.0
30	2043.0
31	1873.0
32	1795.0
33	1769.0
34	2012.0
35	2329.0
36	3294.0
37	4072.0
38	6249.0
39	9405.0
40	10785.0
41	29629.0
>>END_MODULE
>>Per base sequence content	pass
#Base	G	A	T	C
1	22.928162361006603	30.69141376139119	26.785490343616754	19.594933533985454
2	23.555541619632926	30.013796563401478	28.049354069986205	18.381307746979388
3	20.458715116795684	29.849934924758387	28.186204048777686	21.505145909668247
4	19.862745200524767	30.075840332840276	30.283342828619663	19.778071638015295
5	19.385472524485976	29.746412033407548	29.91574942247588	20.952366019630595
6	19.381984298721527	30.463302704341373	30.27252485338853	19.882188143548575
7	19.06315008206061	30.34256384524519	30.511389176362364	20.08289689633184
8	19.295375674206632	30.477798218840157	30.0670025504871	20.15982355646611
9	18.991628256984292	30.80195236154224	29.290648940728897	20.915770440744573
10-14	19.22578096505135	31.04881482263359	30.11994427612788	19.605459936187177
15-19	18.841794476523923	31.335510673320616	30.584589658505994	19.238105191649467
20-24	18.303782783865557	31.799840097822507	31.146017860968715	18.75035925734322
25-29	17.783182446231447	32.483439347983776	31.607067829169743	18.12631037661504
30-34	17.6652651522222	32.758222425475516	31.773584641517182	17.802927780785097
35-39	17.943932411674346	32.54807987711214	31.487557603686632	18.020430107526884
40-44	18.009376950659753	31.972211774945308	31.54471576733663	18.47369550705831
45-49	18.422359039252427	31.441851410215854	31.4491519236853	18.686637626846423
50-54	18.506017445704988	30.983664058052685	31.347068328052856	19.163250168189478
55-59	18.797547991611548	30.724633005323437	31.187610904984673	19.290208098080335
60-64	19.109945788895583	30.74513694504482	30.955603585727953	19.189313680331644
65-69	18.92288824042783	30.926210001422515	30.839321345774557	19.311580412375097
70-74	19.229815307792787	30.806560955042244	30.92437044263298	19.039253294531985
75-79	19.462735707871897	30.634100392626372	30.914044755884994	18.98911914361674
80-84	19.22009871575943	30.467145430302462	31.27090692179934	19.041848932138763
85-89	19.077966977289435	30.478451787239962	31.374058693794833	19.069522541675774
90-94	18.917863999831212	31.08582821697576	31.404941241006817	18.591366542186215
95-99	18.638443871360934	31.160945550169572	31.265342053733164	18.93526852473633
100-104	18.572758761965062	31.17285559983612	31.656300048418935	18.59808558977988
105-109	18.399632958928784	31.262213461571143	31.779639416132817	18.558514163367263
110-114	18.379392636583898	31.252618730985738	31.8592534567249	18.508735175705464
115-119	18.139325666901353	31.535046546194163	31.795157631228975	18.53047015567551
120-124	18.46716248581873	31.3143409386949	32.031808059162145	18.186688516324214
125-129	18.278912950014707	31.506120878872217	32.03222229764895	18.182743873464126
130-134	18.540270500792005	31.99463872304131	31.676617521627882	17.788473254538808
135-139	18.28835367865391	31.89178488947542	31.75057736720554	18.06928406466513
140-144	18.48638433124302	31.881568021074933	31.845774990693815	17.78627265698823
145-149	18.637753597317726	31.657948248296414	31.904754513139714	17.79954364124614
150-154	18.53373520348886	31.896479145969792	31.755038811901194	17.814746838640154
155-159	18.555044196069836	31.802907443933087	31.673241288625903	17.968807071371174
160-164	17.930389072518018	32.32447931185536	31.96607064633029	17.779060969296324
165-169	18.670182626509174	31.624024757006495	31.95310116375365	17.75269145273068
170-174	18.447667207327545	31.860032439652702	31.824253410934073	17.86804694208568
175-179	18.454106280193237	31.91800496148322	31.724768246507377	17.903120511816166
180-184	18.073528151512555	31.767932127860142	32.110720713000255	18.047819007627048
185-189	18.178107140614205	31.624065816743073	31.85329397726559	18.344533065377128
190-194	18.072939509463243	31.884889173779563	31.86464694173611	18.177524375021086
195-199	18.07765975970747	32.46735155841895	31.69075396134425	17.76423472052934
200	17.620590352771778	31.26349892008639	33.4053275737941	17.71058315334773
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	2113.0
1	1740.5
2	1065.5
3	791.0
4	977.0
5	1314.5
6	1533.5
7	1727.5
8	2101.5
9	2433.0
10	2674.5000000000005
11	3056.666666666668
12	3505.5000000000036
13	3882.666666666674
14	4304.166666666641
15	4795.166666666633
16	5221.833333333286
17	5567.6666666665915
18	5957.333333333247
19	6255.333333333276
20	6416.499999999971
21	6625.66666666665
22	6886.833333333315
23	7215.9999999999745
24	7434.66666666664
25	7556.833333333303
26	7766.66666666662
27	7843.666666666618
28	7909.833333333272
29	8024.166666666601
30	8273.833333333423
31	8617.166666666843
32	8840.500000000175
33	8991.00000000018
34	9233.16666666682
35	9556.166666666848
36	9739.333333333509
37	9960.500000000155
38	10200.833333333485
39	10281.333333333487
40	10181.500000000156
41	9963.333333333469
42	9785.333333333476
43	9641.33333333347
44	9457.500000000115
45	9186.666666666784
46	8860.166666666784
47	8569.666666666766
48	8234.499999999998
49	7772.333333333288
50	7287.8333333332885
51	6779.166666666632
52	6367.333333333296
53	6084.666666666628
54	5752.999999999968
55	5352.999999999976
56	5027.999999999977
57	4742.833333333313
58	4449.999999999982
59	4191.166666666651
60	3905.6666666666756
61	3548.1666666666733
62	3284.000000000006
63	3060.6666666666715
64	2759.333333333336
65	2460.0000000000014
66	2211.500000000002
67	1995.499999999999
68	1776.166666666666
69	1561.4999999999998
70	1387.6666666666665
71	1237.8333333333333
72	1064.9999999999998
73	875.8333333333334
74	722.0
75	619.0
76	530.5
77	485.5
78	451.0
79	396.5
80	337.0
81	294.5
82	285.8333333333333
83	284.16666666666663
84	281.5
85	256.5
86	218.5
87	195.0
88	167.5
89	145.5
90	137.0
91	129.0
92	121.5
93	112.0
94	101.5
95	83.0
96	66.0
97	57.5
98	50.5
99	41.5
100	35.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.012540757461750688
3	0.030829362093470442
4	0.02769417272803277
5	0.021946325558063706
6	0.02821670428893905
7	0.02821670428893905
8	0.02090126243625115
9	0.009405568096313016
10-14	0.001985619931443859
15-19	0.005747847169969066
20-24	0.004702784048156508
25-29	0.00982359334503804
30-34	0.006532714902989184
35-39	0.00537605505080372
40-44	0.004528771482129074
45-49	0.0068133483226996425
50-54	0.01954640834746615
55-59	0.03193043680192485
60-64	0.010274251662480204
65-69	0.015376158497441792
70-74	0.012399461036760269
75-79	0.0162834195180988
80-84	0.010759327869465964
85-89	0.0
90-94	0.003164657109402196
95-99	0.0
100-104	0.014895914795367369
105-109	0.01698975517762789
110-114	0.012750455373406192
115-119	0.018575911930623856
120-124	0.0031512936060252733
125-129	0.01809913802855139
130-134	0.012183235867446393
135-139	0.01055645725294591
140-144	0.011452457983794772
145-149	0.012416383417919945
150-154	0.02525082486027877
155-159	0.031037189856316066
160-164	0.015926737009755127
165-169	0.019609979300577403
170-174	0.016694092676063057
175-179	0.010444136922635055
180-184	0.0
185-189	0.0
190-194	0.003373591525538088
195-199	0.0
200	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
30-34	47626.0
35-39	32405.0
40-44	22652.0
45-49	15225.0
50-54	8826.0
55-59	6212.0
60-64	4815.0
65-69	3890.0
70-74	3171.0
75-79	2777.0
80-84	2538.0
85-89	2398.0
90-94	2416.0
95-99	2431.0
100-104	9787.0
105-109	1639.0
110-114	1487.0
115-119	1479.0
120-124	1388.0
125-129	1296.0
130-134	1269.0
135-139	1234.0
140-144	1089.0
145-149	1066.0
150-154	925.0
155-159	929.0
160-164	880.0
165-169	844.0
170-174	731.0
175-179	701.0
180-184	640.0
185-189	632.0
190-194	134.0
195-199	288.0
200-201	5556.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	92.73294101664362
#Duplication Level	Percentage of total
1	90.38668726819247
2	2.7641270035167484
3	0.885810460886554
4	0.7536881612577454
5	0.6034423409951218
6	0.5819163317538683
7	0.480659694359256
8	0.3481033053729889
9	0.2746793049273217
>10	1.5856502995616129
>50	0.4379943135152707
>100	0.8972415156610292
>500	0.0
>1k	0.0
>5k	0.0
>10k+	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	0.0020901262436251147	0.0	0.0	0.0	0.9734762979683973	0.015675946827188363
2	0.0031351893654376723	0.0	0.0	0.0	1.0299097065462754	0.017243541509907197
3	0.004702784048156509	0.0	0.0	0.0	1.0936585569768413	0.01985619931443859
4	0.0052253156090627875	0.0	0.0	0.0	1.1615876598946577	0.0245589833625951
5	0.006792910291781623	0.0	0.0	0.0	1.2300392943733802	0.030829362093470446
6	0.008360504974500459	0.0	0.0	0.0	1.2984909288521027	0.03814480394615835
7	0.010450631218125575	0.0	0.0	0.0	1.362762310843575	0.04493771423793997
8	0.012018225900844412	0.0	0.0	0.0	1.4202407825432657	0.04964049828609648
9	0.01254075746175069	0.0	0.0	0.0	1.4960078588746761	0.059046066382409494
10-14	0.015362427890644594	0.0	0.0	0.0	1.8365939302733885	0.08130591087701697
15-19	0.021319287684976172	0.0	0.0	0.0	2.803277317950004	0.11276231084357495
20-24	0.04472870161357746	0.0	0.0	0.0	4.152140289273471	0.13847086364016387
25-29	0.16386589750020902	0.0	0.0	0.0	5.121122815818076	0.15383329153080846
30-34	0.3242830866984366	0.0	0.0	0.0	5.721929604548114	0.16031268288604633
35-39	0.37799933115960205	0.0	0.0	0.0	6.038897249393863	0.16637404899255914
40-44	0.41185937630632885	0.0	0.0	0.0	6.207883956190955	0.1707633141041719
45-49	0.4432112699607057	0.0	0.0	0.0	6.281456399966558	0.172853440347797
50-54	0.47372711311763227	0.0	0.0	0.0	6.319496697600535	0.17494356659142213
55-59	0.5007942479725775	0.0	0.0	0.0	6.343533149402225	0.1761976423375972
60-64	0.5253532313351726	0.0	0.0	0.0	6.357014463673606	0.1771381991472285
65-69	0.5483446200150489	0.0	0.0	0.0	6.3643299055262945	0.17901931276649108
70-74	0.5760387927430817	0.0	0.0	0.0	6.367465094891731	0.17975085695175988
75-79	0.5992391940473205	0.0	0.0	0.0	6.373317448373881	0.17975085695175988
80-84	0.6167962544937714	0.0	0.0	0.0	6.375930106178413	0.17975085695175988
85-89	0.6278739235849846	0.0	0.0	0.0	6.3790652955438505	0.1809004263857537
90-94	0.6338307833793161	0.0	0.0	0.0	6.381155421787476	0.1813184516344787
95-99	0.6425048072903603	0.0	0.0	0.0	6.3814689407240195	0.1813184516344787
100-104	0.6512833375135859	0.0	0.0	0.0	6.3849176490260025	0.1813184516344787
105-109	0.6614204497951676	0.0	0.0	0.0	6.38732129420617	0.1813184516344787
110-114	0.6709305242036618	0.0	0.0	0.0	6.389620433074158	0.1813184516344787
115-119	0.6773054092467186	0.0	0.0	0.0	6.391606053005601	0.1813184516344787
120-124	0.6835757879775939	0.0	0.0	0.0	6.39306914137614	0.1813184516344787
125-129	0.6948624696931696	0.0	0.0	0.0	6.395263773931946	0.1813184516344787
130-134	0.7043725441016637	0.0	0.0	0.0	6.39808544436084	0.1813184516344787
135-139	0.7128375553883454	0.0	0.0	0.0	6.399026001170471	0.1813184516344787
140-144	0.7190034278070396	0.0	0.0	0.0	6.400384583228828	0.1813184516344787
145-149	0.7243332497282835	0.0	0.0	0.0	6.401220633726277	0.1813184516344787
150-154	0.7287225148398964	0.0	0.0	0.0	6.401534152662821	0.1813184516344787
155-159	0.7346793746342279	0.0	0.0	0.0	6.401534152662821	0.1813184516344787
160-164	0.7400091965554719	0.0	0.0	0.0	6.401534152662821	0.1813184516344787
165-169	0.746593094222891	0.0	0.0	0.0	6.401534152662821	0.1813184516344787
170-174	0.7495192709639662	0.0	0.0	0.0	6.401534152662821	0.1813184516344787
175-179	0.7525499540172227	0.0	0.0	0.0	6.401743165287184	0.1813184516344787
180-184	0.753908536075579	0.0	0.0	0.0	6.402056684223727	0.18173647688320374
185-189	0.7540130423877602	0.0	0.0	0.0	6.40226569684809	0.181840983195385
>>END_MODULE
