##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	SRR6235620
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4142856
Total Bases	621.8 Mbp
Sequences flagged as poor quality	0
Sequence length	30-200
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.599557648153834	31.0	31.0	34.0	30.0	34.0
2	31.8383385760934	33.0	31.0	34.0	30.0	34.0
3	31.936494292826012	34.0	31.0	34.0	30.0	34.0
4	35.511068692708605	37.0	35.0	37.0	33.0	37.0
5	35.38371524378351	37.0	35.0	37.0	33.0	37.0
6	35.322160123354514	37.0	35.0	37.0	32.0	37.0
7	35.180892118866794	37.0	35.0	37.0	32.0	37.0
8	35.331082953402195	37.0	35.0	37.0	33.0	37.0
9	36.7201652676318	39.0	37.0	39.0	32.0	39.0
10-14	36.8264268900488	39.2	36.6	39.4	32.0	39.4
15-19	37.78857662443494	40.0	37.0	41.0	32.4	41.0
20-24	37.74660422664944	40.0	37.0	41.0	32.0	41.0
25-29	37.50075797951944	40.0	36.8	41.0	31.2	41.0
30-34	37.02205670688766	39.0	35.8	41.0	30.4	41.0
35-39	36.611331463675	38.8	35.4	40.6	29.4	41.0
40-44	35.98079268098701	38.2	34.2	40.2	27.6	41.0
45-49	36.13024943764539	38.0	34.6	40.4	29.0	41.0
50-54	35.87559981955633	38.2	33.8	40.4	27.8	41.0
55-59	35.68228995181101	37.8	33.8	40.0	27.6	41.0
60-64	34.972169172621975	36.4	33.4	39.8	27.2	41.0
65-69	34.57208404679649	35.2	32.8	38.8	28.0	41.0
70-74	34.12923494598239	35.0	33.0	37.6	27.2	41.0
75-79	33.41980106554014	34.6	31.8	36.2	26.6	40.6
80-84	33.90368359618687	35.0	33.0	36.0	28.0	41.0
85-89	33.72301809240396	35.0	33.0	35.8	27.6	41.0
90-94	33.623751135864794	35.0	33.0	35.0	27.0	41.0
95-99	33.57093367691112	35.0	32.0	35.0	27.2	41.0
100-104	33.28638300014309	34.6	32.0	34.8	30.2	37.4
105-109	35.61481538850802	37.4	35.4	37.4	32.0	37.4
110-114	36.847207422765784	39.2	37.0	39.4	32.0	39.4
115-119	37.650962190864064	40.0	37.0	41.0	32.0	41.0
120-124	37.55471083004533	40.0	37.0	41.0	31.4	41.0
125-129	37.45720527381219	40.0	36.8	41.0	31.0	41.0
130-134	37.30558477740662	39.8	36.2	41.0	31.0	41.0
135-139	37.152390194304175	39.0	36.0	41.0	30.6	41.0
140-144	36.85970318061443	39.0	35.4	41.0	30.0	41.0
145-149	36.525481261476386	39.0	35.0	40.4	29.8	41.0
150-154	35.94256615081516	37.8	34.4	39.6	29.2	40.6
155-159	36.18692023730843	37.8	34.6	40.0	29.8	41.0
160-164	35.60418226519626	36.8	34.0	39.8	29.0	41.0
165-169	35.12339730910478	35.6	34.0	39.0	29.0	41.0
170-174	34.52685807363294	35.0	33.6	37.4	29.0	39.8
175-179	33.82860484896683	35.0	33.0	36.2	28.6	38.8
180-184	33.28292951514824	35.0	33.0	35.2	27.6	37.0
185-189	32.890622538237736	35.0	32.0	35.0	27.0	36.0
190-194	32.71524438344444	35.0	32.0	35.0	27.0	35.6
195-199	32.77435254482792	34.0	32.0	35.0	27.8	35.0
200	33.00331474780355	34.0	32.0	35.0	29.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	5.0
9	12.0
10	27.0
11	76.0
12	91.0
13	112.0
14	150.0
15	380.0
16	549.0
17	783.0
18	4983.0
19	13183.0
20	16006.0
21	14667.0
22	13447.0
23	15607.0
24	23014.0
25	36764.0
26	56357.0
27	78797.0
28	102101.0
29	125928.0
30	148260.0
31	173052.0
32	201083.0
33	237255.0
34	292200.0
35	381733.0
36	543431.0
37	731742.0
38	596321.0
39	263128.0
40	57895.0
41	13717.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.31666077700987	24.56307918981495	16.558673533427182	13.561586499748
2	15.962511125074197	50.699633497148824	20.744162760770894	12.593692617006088
3	14.670224460168333	22.08492590223842	49.24472940271514	14.00012023487811
4	16.144087817486962	20.796559422655942	21.22491738621002	41.834435373647075
5	43.77426119690905	20.891609700084647	19.84100541248924	15.493123690517063
6	43.902928964038985	20.745174768715806	18.75081183058683	16.601084436658383
7	17.18829183260319	47.61141941068038	20.03401824527179	15.16627051144464
8	15.372071906112678	48.32486339002308	20.074936605196502	16.22812809866774
9	43.050877739389506	20.225018047316563	20.308844513012176	16.415259700281755
10-14	21.377675833447533	31.417990006635655	20.318245746291698	26.886088413625114
15-19	21.421877931724374	25.59359225421702	25.837869021261913	27.146660792796695
20-24	21.444833165258142	31.251059723403984	25.725246695883815	21.57886041545406
25-29	21.400287193138496	25.673914603155996	25.631664129835784	27.29413407386972
30-34	16.19754627422981	25.63740383410892	25.532822155007956	32.63222773665332
35-39	21.547757864972393	25.606575195383197	36.519267984996944	16.326398954647466
40-44	16.23458357773598	25.843273886591795	31.052756176331815	26.869386359340414
45-49	26.872059092861335	25.636714030798768	30.867765365914828	16.62346151042507
50-54	21.8682353710297	20.570696080867123	25.611440996836805	31.949627551266374
55-59	21.441821158845308	20.977432303550454	35.70572791217308	21.875018625431156
60-64	21.77467808895597	25.481308010198106	30.763421742240855	21.980592158605067
65-69	18.136609257576843	41.33495012911822	22.4226122702321	18.105828343072833
70-74	22.063615005742946	29.114832468481556	27.048197402211287	21.773355123564215
75-79	22.58653611834607	28.03829290693341	27.280858773353533	22.094312201366982
80-84	22.480763585106192	27.707662743328537	27.51691083650748	22.294662835057792
85-89	22.500702553166686	27.62339296728053	27.75387347128496	22.12203100826782
90-94	22.650697871604798	27.84458310866711	27.45186654757642	22.05285247215168
95-99	22.230380223496272	28.201186246364173	27.535876021807482	22.032557508332072
100-104	22.389014372934067	30.024767925092377	27.513971921498086	20.072245780475473
105-109	22.24380529482493	27.850464353191924	27.616172892567576	22.28955745941557
110-114	22.4321488890919	27.610814644071606	28.04893642345577	21.908100043380724
115-119	22.20732880294081	27.478737928134905	27.831078992796716	22.482854276127572
120-124	22.056807512310748	27.709110208459908	27.99332961638394	22.240752662845402
125-129	21.99393934314756	28.081103622147	27.877439311792358	22.047517722913078
130-134	22.079346638303026	28.432783244172715	27.36260100468792	22.12526911283633
135-139	22.271126721450962	27.8771566965618	27.561121367430225	22.290595214557012
140-144	22.37568162654267	27.6869899599609	27.625209829206064	22.312118584290364
145-149	22.349682004109653	27.66099133754924	27.641856588769702	22.3474700695714
150-154	22.25622032118231	27.698647049027148	27.676239908444472	22.36889272134607
155-159	22.265911732277353	27.75382678510971	27.704642027526805	22.275619455086133
160-164	22.24577173588619	27.750135182530062	27.65408631256101	22.350006769022734
165-169	22.202729378427	27.75929038550303	27.759960387542094	22.278019848527876
170-174	22.202521968315484	27.806714332087484	27.784170657433833	22.206593042163195
175-179	22.168236308269456	27.729909896673576	27.780586549598564	22.3212672454584
180-184	22.121805299964002	27.775053573982838	27.85812988196883	22.245011244084335
185-189	22.086507744585386	27.916462801226515	27.80550791882107	22.191521535367027
190-194	22.02524210355717	27.89379431780821	27.82199794378513	22.258965634849485
195-199	21.738892174232337	28.05902439422212	28.081053765789292	22.121029665756254
200	21.79051818953475	28.346637476524023	27.861349611754015	22.00149472218721
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	117.0
1	148.5
2	220.0
3	270.0
4	338.0
5	481.0
6	605.5
7	729.1666666666667
8	955.6666666666671
9	1186.4999999999952
10	1443.9999999999823
11	1814.4999999999645
12	2281.500000000074
13	2831.8333333335268
14	3450.1666666669325
15	4092.5000000003047
16	4565.3333333331775
17	4939.999999999479
18	5282.999999999167
19	5584.833333332384
20	6147.999999998796
21	6990.666666665054
22	8267.999999997899
23	9906.166666667164
24	11674.16666667028
25	13753.500000007083
26	16096.333333344242
27	18491.666666672656
28	21299.499999997068
29	24272.16666665359
30	27656.333333308456
31	31194.833333297083
32	35195.16666663685
33	39376.83333332388
34	43726.500000013126
35	48891.000000041844
36	54327.16666674535
37	59905.00000011509
38	65861.6666668138
39	71521.00000009437
40	77277.33333337432
41	82964.83333332452
42	88653.83333326831
43	94861.16666653169
44	116006.66666620964
45	172749.50000062553
46	369230.99999742425
47	426714.99999748677
48	306621.16666571604
49	285478.33333337505
50	308884.9999988508
51	284779.666666533
52	179829.33333409697
53	103145.99999972584
54	72773.33333339586
55	56588.666666745354
56	45835.16666668916
57	38450.99999999062
58	33228.66666664231
59	29523.666666646885
60	26114.666666654975
61	22969.499999995624
62	20543.666666666715
63	18371.833333336566
64	16077.833333338718
65	13858.666666670253
66	11840.666666668169
67	10136.333333333218
68	8518.999999999005
69	6856.833333332624
70	5569.499999999806
71	4431.3333333334895
72	3446.1666666668057
73	2668.5000000000337
74	1982.3333333333044
75	1475.3333333333198
76	1107.1666666666683
77	818.0000000000015
78	559.1666666666667
79	382.83333333333326
80	299.66666666666663
81	237.33333333333334
82	194.0
83	159.5
84	138.0
85	116.0
86	96.5
87	89.0
88	80.0
89	70.5
90	58.5
91	50.0
92	41.0
93	34.0
94	35.0
95	29.5
96	19.5
97	13.0
98	9.5
99	7.0
100	6.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0045137943486329235
3	0.023244833998574896
4	0.023968972129371623
5	0.02302759255933588
6	0.023703454814746158
7	0.023196558123188448
8	0.023051730497029105
9	0.02331724781165457
10-14	0.02383862726582821
15-19	0.016153107904305627
20-24	0.006411036251320345
25-29	0.010070347605613133
30-34	0.023462713647733696
35-39	0.02080795854498735
40-44	0.04370485577570751
45-49	0.10043229509252455
50-54	0.016320588988268395
55-59	0.019810919151377587
60-64	0.013986901879287203
65-69	0.0032322857367620863
70-74	0.0032466944424506643
75-79	0.009807811022146987
80-84	0.012487783726838106
85-89	0.009482628991935735
90-94	0.0015434845913933242
95-99	0.0034012235021922367
100-104	0.008052067874768538
105-109	0.010768655238055161
110-114	0.009412652616000782
115-119	0.008745848227040501
120-124	0.009529854064586969
125-129	0.006400675032816102
130-134	0.008394509015506106
135-139	0.005235262089290172
140-144	0.003812888025965992
145-149	0.004862989438677703
150-154	0.006987839318661888
155-159	0.01242309609241476
160-164	0.004267073181255057
165-169	0.00453643451099819
170-174	0.012261264510401515
175-179	0.003885002566816107
180-184	0.005834947785527965
185-189	0.0032157168250664583
190-194	3.6876705665831616E-4
195-199	5.967288517803107E-4
200	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
30-34	41049.0
35-39	39833.0
40-44	63142.0
45-49	72441.0
50-54	73115.0
55-59	69698.0
60-64	135445.0
65-69	587397.0
70-74	110328.0
75-79	9440.0
80-84	9361.0
85-89	10766.0
90-94	14482.0
95-99	18584.0
100-104	70856.0
105-109	8298.0
110-114	9866.0
115-119	10916.0
120-124	13009.0
125-129	16387.0
130-134	38640.0
135-139	38796.0
140-144	28452.0
145-149	31428.0
150-154	35550.0
155-159	41216.0
160-164	46162.0
165-169	52642.0
170-174	60480.0
175-179	68563.0
180-184	78414.0
185-189	95782.0
190-194	77357.0
195-199	152900.0
200-201	1912061.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	95.41445011997176
#Duplication Level	Percentage of total
1	91.56748403673815
2	6.8955162596811
3	0.9070255368032005
4	0.18527735974227785
5	0.10162312004553815
6	0.05195727728719733
7	0.03434059780232144
8	0.03366706031895532
9	0.028574625945496476
>10	0.1205119089812846
>50	0.007863812693968712
>100	0.015398667475137375
>500	0.0161265382900918
>1k	0.03463319819528751
>5k	0.0
>10k+	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	0.48975875579551886	0.0	0.0	2.413793769322419E-5	0.006903450180262119	1.6896556385256935E-4
2	0.4917380666863632	0.0	0.0	2.413793769322419E-5	0.007627588311058845	2.1724143923901773E-4
3	0.492148411627148	0.0	0.0	2.413793769322419E-5	0.008424140254935243	4.3448287847803546E-4
4	0.49434496395723143	0.0	0.0	2.413793769322419E-5	0.009413795700357434	4.3448287847803546E-4
5	0.4957691022811317	0.0	0.0	2.413793769322419E-5	0.010137933831154161	4.827587538644838E-4
6	0.49721737854272513	0.0	2.413793769322419E-5	2.413793769322419E-5	0.010910347837337334	5.310346292509322E-4
7	0.4991001376827966	0.0	2.413793769322419E-5	2.413793769322419E-5	0.011731037718906956	6.517243177170532E-4
8	0.5013208279505732	0.0	2.413793769322419E-5	2.413793769322419E-5	0.01272069316432915	6.517243177170532E-4
9	0.5023104833959954	0.0	2.413793769322419E-5	4.827587538644838E-5	0.013951727986683583	7.482760684899499E-4
10-14	0.5055884153347352	4.8275875386448375E-5	2.413793769322419E-5	6.758622554102773E-5	0.02018414349907407	0.0011248278965042473
15-19	0.5108746236895513	8.689657569560709E-5	2.413793769322419E-5	7.241381307967257E-5	0.043062080844711956	0.0020468971163854115
20-24	0.5127863483548547	9.655175077289676E-5	2.413793769322419E-5	1.1103451338883128E-4	0.07609243478411994	0.0027034490216411096
25-29	0.5139063486638203	1.4965521369798997E-4	2.413793769322419E-5	1.641379763139245E-4	0.10118140722245716	0.0030268973867303138
30-34	0.5155573836020368	1.9310350154579353E-4	2.413793769322419E-5	1.7379315139121418E-4	0.11953589504438485	0.003205518125660173
35-39	0.5172132461277921	2.1724143923901773E-4	2.413793769322419E-5	1.9793108908443838E-4	0.13067313949603848	0.003321380226587649
40-44	0.5210077299331668	2.5586213954817644E-4	2.413793769322419E-5	2.1724143923901773E-4	0.13582900298731118	0.0033889664521286763
45-49	0.5233877305897188	3.3310354016649385E-4	7.241381307967257E-5	2.3655178939359709E-4	0.1381993484687858	0.0034903457904402182
50-54	0.5253911794182564	3.8620700309158706E-4	1.1103451338883128E-4	2.606897270868213E-4	0.13960417644253142	0.003557932015981245
55-59	0.5262987658755216	4.1034494078481126E-4	1.255172760047658E-4	2.9931042739598E-4	0.1404634870244102	0.003615863066444984
60-64	0.5299339392921212	4.1034494078481126E-4	1.4482762615934515E-4	3.2344836508920415E-4	0.15580073263468486	0.0036448285916768535
65-69	0.5946139571348847	4.1034494078481126E-4	1.4482762615934515E-4	3.379311277051387E-4	0.2181779912215148	0.0036448285916768535
70-74	1.0721782268077868	4.3448287847803546E-4	1.5448280123663482E-4	3.427587152437835E-4	0.21902281904077764	0.003654483766754143
75-79	1.1262278968904544	4.731035787871941E-4	1.6896556385256935E-4	3.717242404756525E-4	0.21937523293109873	0.0036979320546019464
80-84	1.1325472089785404	5.020691040190632E-4	1.6896556385256935E-4	3.9586217816887676E-4	0.2196697157709561	0.0037413803424497494
85-89	1.1342947956675298	5.600001544828012E-4	1.6896556385256935E-4	4.1034494078481126E-4	0.2199931641360453	0.0037462079299883945
90-94	1.1350575544986357	5.937932672533151E-4	1.7862073892985903E-4	4.3448287847803546E-4	0.2202779918008253	0.003770345867681619
95-99	1.1353954856263406	6.27586380023829E-4	1.9310350154579353E-4	4.586208161712596E-4	0.22063523327868503	0.003789656217836198
100-104	1.1364575548848426	6.27586380023829E-4	1.9310350154579353E-4	4.586208161712596E-4	0.22118557825809057	0.003789656217836198
105-109	1.1365734169857702	6.372415551011187E-4	1.9310350154579353E-4	4.682759912485493E-4	0.22160557837395264	0.0038137941555294224
110-114	1.1366216928611568	6.951726055648567E-4	1.9310350154579353E-4	4.827587538644838E-4	0.22215109576581954	0.0038379320932226462
115-119	1.136660313561466	7.048277806421463E-4	2.0275867662308318E-4	4.827587538644838E-4	0.2231310960361644	0.003842759680761291
120-124	1.1368147963627024	7.241381307967257E-4	2.1724143923901773E-4	4.875863414031286E-4	0.22467592404853076	0.0038765527935318055
125-129	1.136916175701014	7.482760684899499E-4	2.1724143923901773E-4	5.117242790963529E-4	0.22937799431117084	0.003924828668918254
130-134	1.136983761926555	7.724140061831741E-4	2.1724143923901773E-4	5.551725669441564E-4	0.2340124783482699	0.003982759719381991
135-139	1.1370465205645575	7.820691812604637E-4	2.1724143923901773E-4	5.551725669441564E-4	0.23484765099245544	0.00403103559476844
140-144	1.1370996240274824	7.965519438763982E-4	2.413793769322419E-4	5.600001544828012E-4	0.23560558223602265	0.004045518357384374
145-149	1.137133417140253	8.255174691082674E-4	2.413793769322419E-4	5.793105046373806E-4	0.2361559272154282	0.004055173532461664
150-154	1.1371478999028688	8.496554068014916E-4	2.413793769322419E-4	5.793105046373806E-4	0.23663385838175408	0.004069656295077598
155-159	1.1372106585408712	8.786209320333605E-4	2.413793769322419E-4	5.793105046373806E-4	0.2370248929723843	0.004103449407848112
160-164	1.1372396240661033	9.172416323425192E-4	2.5586213954817644E-4	5.793105046373806E-4	0.23737247927516672	0.004103449407848112
165-169	1.1372782447664125	9.22069219881164E-4	2.655173146254661E-4	5.986208547919599E-4	0.23750765172624874	0.004171035633389141
170-174	1.1373023827041056	9.703450952676124E-4	2.655173146254661E-4	6.27586380023829E-4	0.2376283414147149	0.004209656333698299
175-179	1.1373313482293375	9.896554454221918E-4	2.655173146254661E-4	6.517243177170532E-4	0.23774420351564235	0.004262759796623392
180-184	1.137350658579492	9.896554454221918E-4	2.655173146254661E-4	6.517243177170532E-4	0.23790834149195628	0.004272414971700682
185-189	1.1373554861670307	9.896554454221918E-4	2.655173146254661E-4	6.517243177170532E-4	0.23805799670565425	0.004296552909393906
>>END_MODULE
