FastQCFastQC Report
Tue 12 Sep 2023
stdin:SRR6235621

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6235621
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4116090
Total Bases398.1 Mbp
Sequences flagged as poor quality0
Sequence length30-200
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGC219034153.21411825300224TruSeq Adapter, Index 12 (100% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTC285760.694251097522163TruSeq Adapter, Index 12 (100% over 44bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATATCGTATGC244780.5946905922853971TruSeq Adapter, Index 12 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCG215980.5247212767456494TruSeq Adapter, Index 12 (100% over 45bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTAT152890.3714447448913897TruSeq Adapter, Index 12 (100% over 48bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTA125580.30509536963477474TruSeq Adapter, Index 12 (100% over 47bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGT121510.2952073448345396TruSeq Adapter, Index 12 (100% over 46bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAA121140.29430843348906366TruSeq Adapter, Index 12 (100% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATG82930.2014776158927526TruSeq Adapter, Index 12 (100% over 49bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGGATGC82880.2013561413866072TruSeq Adapter, Index 12 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTA70040.17016148820846969TruSeq Adapter, Index 12 (100% over 39bp)
GATCGGAAGAGCACACGTTTGAACTCCAGTCACCTTGTAATCTCGTATGC69280.1683150757150597TruSeq Adapter, Index 12 (98% over 50bp)
GATCGGAAGAGCACATGTCTGAACTCCAGTCACCTTGTAATCTCGTATGC68810.167173215357293TruSeq Adapter, Index 12 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAAGCTCGTATGC68500.16642007341919152TruSeq Adapter, Index 12 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCTAGTCACCTTGTAATCTCGTATGC65960.16024916850700544TruSeq Adapter, Index 12 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTTCAGTCACCTTGTAATCTCGTATGC60390.1467169085224084TruSeq Adapter, Index 12 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGGCACCTTGTAATCTCGTATGC58170.14132344044955286TruSeq Adapter, Index 12 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAATTCCAGTCACCTTGTAATCTCGTATGC56460.13716901233938034TruSeq Adapter, Index 12 (98% over 50bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGCC55550.13495817632753415TruSeq Adapter, Index 12 (100% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATTC55410.13461804771032704TruSeq Adapter, Index 12 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTG54100.13143541564931768TruSeq Adapter, Index 12 (100% over 37bp)
GATTGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGC52830.12834996319322464TruSeq Adapter, Index 12 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAACCTCGTATGC51450.12499726682361173TruSeq Adapter, Index 12 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAAT50790.12339380334249252TruSeq Adapter, Index 12 (100% over 41bp)
GATCGGAAGAGTACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGC50360.1223491225896421TruSeq Adapter, Index 12 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGAAATCTCGTATGC48130.11693135961555748TruSeq Adapter, Index 12 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGCATGC45010.10935135043208483TruSeq Adapter, Index 12 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCT41800.10155268713755045TruSeq Adapter, Index 12 (100% over 43bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)