FastQCFastQC Report
Wed 13 Sep 2023
stdin:SRR6235624

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6235624
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences94848
Total Bases8.9 Mbp
Sequences flagged as poor quality0
Sequence length30-200
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGC23952.5250927800269904TruSeq Adapter, Index 12 (100% over 50bp)
TCTGCAGGATATCGCGGCCGCGTCTTCAGAGGGGGATAGCATGACCTCAC3970.41856443994601883No Hit
TCTGCAGGATATCGCGGCCGCCATCTGCCCTACGTTTGAGGGTTATAAGC3610.38060897435897434No Hit
TCTGCAGGATATCGCGGCCGCAGAAGACCTAAGCTAGCGTTTAGAGTGCA2390.2519821187584345No Hit
TCTGCAGGATATCGCGGCCGCTCTAAACGTGGTAAATTGTTAACTCCGAG2060.21718960863697706No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)