##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	SRR6235625
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	466733
Total Bases	34.8 Mbp
Sequences flagged as poor quality	0
Sequence length	30-200
%GC	39
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.499716111781254	34.0	30.0	41.0	16.0	41.0
2	32.60814641347408	34.0	30.0	41.0	16.0	41.0
3	32.62857136735564	34.0	31.0	41.0	16.0	41.0
4	34.54034962173234	37.0	35.0	41.0	19.0	41.0
5	34.416775758302926	37.0	35.0	41.0	19.0	41.0
6	34.28606505218187	37.0	35.0	41.0	17.0	41.0
7	33.77004625771051	37.0	33.0	41.0	17.0	41.0
8	33.347757711582425	37.0	32.0	41.0	15.0	41.0
9	33.64744725571151	39.0	32.0	41.0	11.0	41.0
10-14	33.094483569835425	39.4	30.8	41.0	10.0	41.0
15-19	32.86106017787472	41.0	27.2	41.0	10.0	41.0
20-24	33.17388314089639	41.0	27.0	41.0	10.4	41.0
25-29	34.22512828533659	41.0	30.8	41.0	14.8	41.0
30-34	35.343124322492024	41.0	33.2	41.0	18.4	41.0
35-39	36.0372578217785	41.0	35.0	41.0	23.6	41.0
40-44	36.659861728665675	41.0	37.2	41.0	25.8	41.0
45-49	37.4775435988674	41.0	38.6	41.0	29.8	41.0
50-54	38.38863324105413	41.0	39.2	41.0	32.2	41.0
55-59	38.57487245026549	41.0	39.2	41.0	33.4	41.0
60-64	38.209989068852515	41.0	39.0	41.0	33.0	41.0
65-69	38.05125738749915	41.0	38.6	41.0	32.6	41.0
70-74	37.98139616476361	41.0	37.8	41.0	32.0	41.0
75-79	38.117652714644535	41.0	36.6	41.0	32.0	41.0
80-84	38.25607922370758	41.0	36.0	41.0	33.0	41.0
85-89	38.02260926093264	41.0	35.2	41.0	32.2	41.0
90-94	38.02390053508542	41.0	35.0	41.0	32.4	41.0
95-99	37.81062842943852	41.0	35.0	41.0	32.0	41.0
100-104	35.90521706971975	37.2	34.0	40.2	30.6	41.0
105-109	36.302249283490156	37.4	35.0	40.0	31.0	41.0
110-114	36.73831050487295	39.0	35.2	40.2	31.0	41.0
115-119	37.06459124539697	39.0	36.0	41.0	31.0	41.0
120-124	37.028927986130924	39.0	35.6	41.0	31.0	41.0
125-129	36.94704365324701	39.0	35.2	41.0	31.0	41.0
130-134	36.894754263210714	39.0	35.0	41.0	31.0	41.0
135-139	36.79676763915462	39.0	35.0	40.4	31.0	41.0
140-144	36.79462266887911	39.0	35.0	40.6	31.0	41.0
145-149	36.530495691227664	38.4	35.0	40.4	30.2	41.0
150-154	36.208490242226866	37.8	34.6	39.8	30.2	41.0
155-159	36.61259140432659	38.0	35.0	40.0	31.0	41.0
160-164	36.39372618232288	37.8	34.8	40.0	31.0	41.0
165-169	36.020922073448006	37.0	34.0	40.0	30.0	41.0
170-174	35.315136563588986	36.6	33.6	39.4	29.0	41.0
175-179	34.613166625141446	35.2	33.0	39.0	27.8	40.6
180-184	34.308864812445904	35.0	33.0	38.0	27.8	40.6
185-189	31.97417075549385	34.4	29.6	36.4	19.2	40.0
190-194	33.893051448407434	35.0	32.8	36.8	27.8	39.8
195-199	33.79668793380838	35.0	33.0	36.0	28.8	39.0
200	33.958639915908776	35.0	33.0	36.0	29.0	39.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	2.0
3	0.0
4	0.0
5	0.0
6	0.0
7	1.0
8	1.0
9	1.0
10	2.0
11	1.0
12	9.0
13	19.0
14	24.0
15	455.0
16	2660.0
17	5116.0
18	10132.0
19	17577.0
20	19978.0
21	18424.0
22	16289.0
23	14739.0
24	13029.0
25	11775.0
26	10068.0
27	8707.0
28	7257.0
29	5703.0
30	4895.0
31	4566.0
32	4598.0
33	4943.0
34	5862.0
35	7450.0
36	10374.0
37	14025.0
38	19046.0
39	38957.0
40	53975.0
41	136073.0
>>END_MODULE
>>Per base sequence content	pass
#Base	G	A	T	C
1	21.706079090525378	30.611637110026972	27.27566842570989	20.406615373737765
2	22.561803360863646	29.228670821671138	28.355216309073818	19.854309508391403
3	21.318295815143077	28.761305680544186	28.117581237808658	21.80281726650408
4	21.374630362315237	28.299050136606407	29.366591891874354	20.959727609204005
5	21.36796191779754	28.11464510176767	28.785804936998904	21.731588043435888
6	21.5818211782666	28.715694608285336	28.308236763583373	21.394247449864686
7	20.96064977509137	28.948640005622718	28.766121380376724	21.324588838909193
8	21.164664706954902	29.253589963666766	28.543602104392452	21.03814322498588
9	21.2084843789534	29.19959069342535	28.063026047392686	21.528898880228567
10-14	20.90335267839633	29.489116316981324	28.648320640249004	20.959210364373345
15-19	20.682398695078845	30.02625197495938	28.648954927150434	20.642394402811345
20-24	20.262232325488704	30.521924234261462	28.662120196750895	20.553723243498943
25-29	19.898439130548446	31.30464446624402	28.4986708117284	20.298245591479137
30-34	19.741578770035158	31.668920665765064	28.726728509308597	19.862772054891177
35-39	19.837463417503713	31.81928614497698	28.72009059223201	19.62315984528729
40-44	19.583027484518713	31.077711521286727	29.34332881275296	19.995932181441596
45-49	19.99954515654457	30.373975425906004	29.76291676992136	19.86356264762807
50-54	19.718186577251252	30.218354062829818	29.663547563379407	20.39991179653953
55-59	19.59190172925603	30.206564351069826	30.26664000658661	19.934893913087535
60-64	19.505523377731855	30.445121286440692	30.1605791811827	19.888776154644756
65-69	19.058015080854364	31.302838534305344	29.963940715490473	19.675205669349815
70-74	19.433361868063916	30.557945675447883	30.576871023751313	19.431821432736893
75-79	19.648480790543037	30.323120613224972	30.57766900628002	19.450729589951976
80-84	19.420319608756063	30.083965426432187	30.985943031578383	19.50977193323337
85-89	19.421044490064524	30.208095728754547	31.089821740851733	19.2810380403292
90-94	19.26655974542023	31.172903504280374	30.78005961071074	18.78047713958865
95-99	18.962789654566283	31.52302386335396	30.747480012849966	18.766706469229792
100-104	19.161861689364578	31.06008827100799	30.997375137241782	18.780674902385645
105-109	19.026893960725	30.76293412065678	31.05898489980129	19.151187018816923
110-114	19.27619385409255	30.819937325498657	31.029501134326292	18.874367686082497
115-119	19.210579564193033	30.78907156695607	31.041652615004615	18.95869625384628
120-124	19.129506048084526	30.829473031245936	30.924271378402985	19.116749542266557
125-129	18.94932004825377	31.079691586086287	31.180355210354588	18.79063315530535
130-134	19.163336247145875	31.415966718218733	30.588892234738296	18.83180479989709
135-139	19.199234327643964	31.390652416653374	30.70314244696124	18.706970808741428
140-144	19.37156219921958	31.17448999801941	30.730073351332383	18.72387445142863
145-149	19.53533287889116	31.12057865636598	30.656290237067335	18.68779822767553
150-154	19.702525562920464	31.066939997772653	30.628895277613932	18.601639161692948
155-159	19.69021271999892	31.220833689591704	30.53771763636445	18.55123595404492
160-164	19.701121042042764	31.223898511016117	30.468338801631145	18.60664164530997
165-169	19.63606428598331	31.30202252269218	30.518086485846645	18.543826705477866
170-174	19.754056705234422	31.151832460732987	30.388947324879155	18.70516350915344
175-179	19.67656731491312	31.089287101535596	30.53318486676852	18.700960716782763
180-184	19.450958166554496	31.391732492649925	30.467925330310653	18.68938401048493
185-189	19.967388662856784	31.066218260780946	30.509630535500104	18.45676254086216
190-194	18.761038355160117	31.92042387283815	30.808257522743176	18.510280249258557
195-199	18.07403148385298	32.25918859821063	31.127547064614202	18.539232853322183
200	17.860244047347013	32.69046204469631	31.470225309629356	17.979068598327316
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	189.0
1	265.5
2	427.5
3	538.5
4	742.0
5	1125.0
6	1481.0
7	1906.8333333333333
8	2565.5
9	3226.5
10	4087.833333333333
11	5478.166666666667
12	7228.333333333335
13	9057.833333333328
14	10798.999999999995
15	12539.166666666662
16	13577.833333333328
17	13485.166666666659
18	13013.16666666666
19	12574.166666666668
20	12329.0
21	12234.5
22	12294.166666666666
23	12470.0
24	12588.166666666666
25	12729.333333333332
26	12882.166666666666
27	12939.166666666666
28	13054.5
29	13340.333333333334
30	13791.666666666668
31	14376.5
32	15078.000000000002
33	15733.333333333336
34	16551.0
35	17797.333333333336
36	19183.166666666664
37	20479.666666666664
38	21429.833333333332
39	22176.666666666664
40	22457.833333333332
41	22291.0
42	22156.166666666664
43	21975.166666666664
44	21847.833333333332
45	21689.83333333333
46	21210.166666666664
47	20665.166666666664
48	20202.33333333333
49	19464.333333333336
50	18517.166666666668
51	17638.333333333332
52	16840.33333333333
53	16102.833333333328
54	15312.999999999996
55	14518.666666666664
56	13803.999999999998
57	13082.499999999996
58	12381.999999999998
59	11833.333333333332
60	11401.333333333332
61	10913.499999999998
62	10320.333333333332
63	9685.166666666666
64	8990.333333333332
65	8244.666666666666
66	7476.333333333334
67	6823.666666666667
68	6234.833333333334
69	5546.666666666667
70	4902.166666666667
71	4365.5
72	3731.1666666666665
73	3128.333333333333
74	2677.5
75	2317.3333333333335
76	1971.8333333333335
77	1649.6666666666665
78	1382.6666666666665
79	1146.1666666666665
80	954.5
81	810.5
82	688.5
83	584.5
84	540.5
85	502.0
86	444.0
87	406.5
88	380.0
89	321.5
90	272.0
91	256.0
92	222.5
93	183.0
94	167.0
95	135.5
96	97.0
97	82.0
98	70.5
99	48.5
100	38.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	4.2851051886196175E-4
2	0.4096560560320354
3	0.8145984963565894
4	0.9547214360244508
5	1.0702050208577496
6	1.1957586028843044
7	2.4504374021121285
8	3.6438391971426918
9	4.3116728407890585
10-14	4.950496322308472
15-19	4.559523324898818
20-24	2.167191949144372
25-29	0.012255400839452106
30-34	0.10512266719748563
35-39	1.1254168910830966
40-44	2.1839637513647623
45-49	1.2441635062409533
50-54	0.02877567092748531
55-59	0.44361456244741926
60-64	1.688753360318883
65-69	1.7722408698396304
70-74	0.8881347207893755
75-79	0.006118022424283701
80-84	0.00867563393223719
85-89	0.052409390714628246
90-94	0.005641963798339288
95-99	0.006666989914662529
100-104	0.07138744125976128
105-109	0.09463855506416735
110-114	0.11276465670506859
115-119	0.09851644457869117
120-124	0.0669892817149256
125-129	0.0439703914713626
130-134	0.04821544413729421
135-139	0.058029792878277725
140-144	0.07499765632323989
145-149	0.04428848839982284
150-154	0.020619321954216858
155-159	0.028342630915961852
160-164	0.04572586117038537
165-169	0.058612864717206364
170-174	0.024222086998647106
175-179	0.00712070896367791
180-184	0.014166814237648309
185-189	0.7631628080657207
190-194	0.0
195-199	8.711560240439063E-4
200	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
30-34	96220.0
35-39	63628.0
40-44	37902.0
45-49	24433.0
50-54	16404.0
55-59	15997.0
60-64	12389.0
65-69	11928.0
70-74	10680.0
75-79	9638.0
80-84	9507.0
85-89	12351.0
90-94	9653.0
95-99	9837.0
100-104	23727.0
105-109	7157.0
110-114	6673.0
115-119	6225.0
120-124	5980.0
125-129	5632.0
130-134	5808.0
135-139	5422.0
140-144	4868.0
145-149	4441.0
150-154	4226.0
155-159	3881.0
160-164	3577.0
165-169	3399.0
170-174	3140.0
175-179	2766.0
180-184	2551.0
185-189	2420.0
190-194	770.0
195-199	1622.0
200-201	21881.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	90.26261497811761
#Duplication Level	Percentage of total
1	87.67965842622658
2	3.0199305054674883
3	0.7872494196821684
4	0.5011034803956912
5	0.4233817904830136
6	0.46961106980556316
7	0.4645851892795632
8	0.4794853722206561
9	0.4639250278010304
>10	5.356625879173897
>50	0.06442392775571208
>100	0.023794203282597494
>500	0.0
>1k	0.26622570842604365
>5k	0.0
>10k+	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGC	1186	0.25410673768514336	TruSeq Adapter, Index 4 (100% over 50bp)
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	0.07241827768767153	0.0	0.0	0.0	0.1199829452813493	0.004070849929188637
2	0.08655912481011628	0.0	0.0	0.0	0.13476655818208697	0.0051421262263435415
3	0.11076996912581712	0.0	0.0	0.0	0.14847889478566975	0.006427657782929427
4	0.11826890320590144	0.0	0.0	0.0	0.16690484709673412	0.008784465636670217
5	0.12233975313509009	0.0	0.0	0.0	0.18018867318145493	0.00985574193382512
6	0.12426805046996892	0.0	0.0	0.0	0.19625781763877848	0.01221254978756591
7	0.12641060306427873	0.0	0.0	0.0	0.21232696209610205	0.014355102381875718
8	0.1279103898802956	0.0	0.0	0.0	0.22903887233171857	0.016069144457323565
9	0.12876741091801952	0.0	0.0	0.0	0.24810759042107586	0.017354676013909453
10-14	0.13283826084720815	0.0	0.0	0.0	0.3694617693627834	0.025753482183603904
15-19	0.1402514928235201	0.0	0.0	0.0	0.8538072088324586	0.03993718035793484
20-24	0.2329383180533624	0.0	0.0	0.0	1.6826322544152652	0.05120700700400442
25-29	0.7963867993049559	0.0	0.0	0.0	2.3755337634150573	0.06029143000387802
30-34	1.5356102953937263	0.0	0.0	0.0	2.8162568320645844	0.0652621520226768
35-39	1.717470159598743	0.0	0.0	0.0	3.0273411136559876	0.06706189620189702
40-44	1.8231408535501026	0.0	0.0	0.0	3.1139859405698758	0.06813317249905192
45-49	1.908243042596088	0.0	0.0	0.0	3.156279928781552	0.06860453406980008
50-54	1.983146681293159	0.0	0.0	0.0	3.1810906878236596	0.06937585300375163
55-59	2.0415955160659305	0.0	0.0	0.0	3.1929604291961358	0.06976151247072737
60-64	2.09297392727748	0.0	0.0	0.0	3.201273533262058	0.07006146983393075
65-69	2.1440095300739395	0.0	0.0	0.0	3.2072726805261254	0.07010432088581695
70-74	2.221012870313434	0.0	0.0	0.0	3.209886594691183	0.07083278876788228
75-79	2.2658350705863954	0.0	0.0	0.0	3.211557785714745	0.07173266085749239
80-84	2.302301315741548	0.0	0.0	0.0	3.2130575725307615	0.07241827768767153
85-89	2.3329826688920647	0.0	0.0	0.0	3.215114423021299	0.07250397979144393
90-94	2.3505087491135184	0.0	0.0	0.0	3.215928593007136	0.07263253294710252
95-99	2.3645638941321914	0.0	0.0	0.0	3.216185699318454	0.07263253294710252
100-104	2.3816614638347837	0.0	0.0	0.0	3.217299826667495	0.07280393715464731
105-109	2.3987590335373756	0.0	0.0	0.0	3.2182854008608777	0.0728467882065335
110-114	2.413971156956975	0.0	0.0	0.0	3.2193138261061462	0.0728467882065335
115-119	2.4291404293246885	0.0	0.0	0.0	3.220427953455187	0.0728467882065335
120-124	2.4440954464329714	0.0	0.0	0.0	3.222270548686294	0.0728467882065335
125-129	2.4574221235695783	0.0	0.0	0.0	3.224070292865514	0.0728467882065335
130-134	2.4718200770033403	0.0	0.0	0.0	3.225570079681531	0.0728467882065335
135-139	2.486860796215395	0.0	0.0	0.0	3.2265985049267996	0.0728467882065335
140-144	2.497016495512424	0.0	0.0	0.0	3.2272841217569783	0.0728467882065335
145-149	2.5097004068707376	0.0	0.0	0.0	3.2277554833277273	0.0728467882065335
150-154	2.520456020894173	0.0	0.0	0.0	3.228698206469223	0.0728467882065335
155-159	2.5304831670355425	0.0	0.0	0.0	3.229041014884313	0.0728467882065335
160-164	2.5390962284646683	0.0	0.0	0.0	3.229041014884313	0.0728467882065335
165-169	2.5467237157004115	0.0	0.0	0.0	3.2292552701437445	0.0728467882065335
170-174	2.553665586105975	0.0	0.0	0.0	3.2293838232994028	0.0728467882065335
175-179	2.55957903126627	0.0	0.0	0.0	3.2297266317144917	0.0728467882065335
180-184	2.5607788607190836	1.714042075447847E-4	0.0	0.0	3.2298980359220364	0.0728467882065335
185-189	2.5607788607190836	2.1425525943098088E-4	0.0	0.0	3.229983738025809	0.0728467882065335
>>END_MODULE
