FastQCFastQC Report
Wed 13 Sep 2023
stdin:SRR6235627

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6235627
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3022236
Total Bases322.5 Mbp
Sequences flagged as poor quality0
Sequence length30-200
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCGGAAGAGCACACGTCTGAACTCCAGTCACAGCATGATCTCGTATGC345561.1433918463018773TruSeq Adapter, Index 13 (97% over 35bp)
GAGACGGAAGAGCACACGTCTGAACTCCAGTCACAGCATGATCTCGTATG209480.6931291930875021TruSeq Adapter, Index 13 (96% over 33bp)
AGACGGAAGAGCACACGTCTGAACTCCAGTCACAGCATGATCTCGTATGC194830.6446551493662308TruSeq Adapter, Index 13 (96% over 33bp)
GAAGAGCACACGTCTGAACTCCAGTCACAGCATGATCTCGTATGCCGTCT185740.6145780805999267TruSeq Adapter, Index 1 (96% over 32bp)
CAGACGGAAGAGCACACGTCTGAACTCCAGTCACAGCATGATCTCGTATG178350.590125986190357TruSeq Adapter, Index 13 (96% over 33bp)
CAGGCGGAAGAGCACACGTCTGAACTCCAGTCACAGCATGATCTCGTATG166700.5515783678044998TruSeq Adapter, Index 13 (96% over 33bp)
GACCGGAAGAGCACACGTCTGAACTCCAGTCACAGCATGATCTCGTATGC146660.4852698465639348TruSeq Adapter, Index 13 (97% over 35bp)
GACGGAAGAGCACACGTCTGAACTCCAGTCACAGCATGATCTCGTATGCC146460.4846080848749072TruSeq Adapter, Index 1 (97% over 34bp)
AAGACGGAAGAGCACACGTCTGAACTCCAGTCACAGCATGATCTCGTATG124040.41042459953491384TruSeq Adapter, Index 13 (96% over 33bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGCATGATCTCGTATGC115020.3805791473597694TruSeq Adapter, Index 1 (97% over 37bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACAGCATGATCTCGTATGCCGT108910.3603623277599764TruSeq Adapter, Index 1 (97% over 34bp)
GGCGGAAGAGCACACGTCTGAACTCCAGTCACAGCATGATCTCGTATGCC107510.3557299959367832TruSeq Adapter, Index 1 (97% over 34bp)
GAGGCGGAAGAGCACACGTCTGAACTCCAGTCACAGCATGATCTCGTATG86360.28574869732211516TruSeq Adapter, Index 13 (96% over 33bp)
GGAAGAGCACACGTCTGAACTCCAGTCACAGCATGATCTCGTATGCCGTC61820.20455053807842935TruSeq Adapter, Index 1 (96% over 33bp)
AGGCGGAAGAGCACACGTCTGAACTCCAGTCACAGCATGATCTCGTATGC56420.1866829724746843TruSeq Adapter, Index 13 (96% over 33bp)
GGAGCGGAAGAGCACACGTCTGAACTCCAGTCACAGCATGATCTCGTATG53000.17536684759231244TruSeq Adapter, Index 13 (97% over 35bp)
TAGACGGAAGAGCACACGTCTGAACTCCAGTCACAGCATGATCTCGTATG46000.15220518847634665TruSeq Adapter, Index 13 (96% over 33bp)
CAGCCGGAAGAGCACACGTCTGAACTCCAGTCACAGCATGATCTCGTATG35280.11673476194446761TruSeq Adapter, Index 13 (96% over 33bp)
GGACGGAAGAGCACACGTCTGAACTCCAGTCACAGCATGATCTCGTATGC30700.1015804192657357TruSeq Adapter, Index 1 (97% over 34bp)
AGGGCGGAAGAGCACACGTCTGAACTCCAGTCACAGCATGATCTCGTATG30370.10048851247884016TruSeq Adapter, Index 1 (97% over 34bp)
AAGGCGGAAGAGCACACGTCTGAACTCCAGTCACAGCATGATCTCGTATG30230.10002527929652086TruSeq Adapter, Index 13 (96% over 33bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)