FastQCFastQC Report
Wed 13 Sep 2023
stdin:SRR6235679

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6235679
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences570164
Total Bases34 Mbp
Sequences flagged as poor quality0
Sequence length30-200
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCAGAAATCTCGTATGCCGTCTTCTGCTTGAAAAAA48020.8422138191818495TruSeq Adapter, Index 25 (96% over 27bp)
GCAGAAATCTCGTATGCCGTCTTCTGCTTGAAAAAAA44290.7767940452220764TruSeq Adapter, Index 25 (96% over 27bp)
GCAGAAATCTCGTATGCCGTCTTCTGCTTGAAAAA15660.27465781775068226TruSeq Adapter, Index 25 (96% over 27bp)
GCAGAAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAA15380.26974695000035076TruSeq Adapter, Index 25 (96% over 27bp)
GCAGAAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA7740.13575041566987744TruSeq Adapter, Index 25 (96% over 27bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6240.10944219557881593No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6170.10821447864123304No Hit
GCAGAAATCTCGTATGCCGTCTTCTGCTTGAAAA6070.10646059730182895TruSeq Adapter, Index 25 (96% over 27bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)