FastQCFastQC Report
Wed 13 Sep 2023
stdin:SRR6235733

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6235733
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4780328
Total Bases372.6 Mbp
Sequences flagged as poor quality0
Sequence length30-200
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGC350028273.22263242187566TruSeq Adapter, Index 6 (100% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTC347790.7275442187230666TruSeq Adapter, Index 6 (100% over 44bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCG310190.6488885281512063TruSeq Adapter, Index 6 (100% over 45bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATA199860.4180884658960641TruSeq Adapter, Index 6 (100% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGT190940.3994286584518887TruSeq Adapter, Index 6 (100% over 46bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCCAATATCTCGTATGC171280.35830177343479364TruSeq Adapter, Index 6 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTAT139140.29106789324916615TruSeq Adapter, Index 6 (100% over 48bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTA116630.24397907423925722TruSeq Adapter, Index 6 (100% over 47bp)
GATTGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGC114870.24029731851036162TruSeq Adapter, Index 6 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATAGCTCGTATGC106050.2218467017326008TruSeq Adapter, Index 6 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAA105920.2215747538662619TruSeq Adapter, Index 6 (100% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATACCTCGTATGC103340.21617763467276724TruSeq Adapter, Index 6 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATATCGTATGC95650.20009087242549048TruSeq Adapter, Index 6 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGGATGC94810.1983336708276085TruSeq Adapter, Index 6 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATC93750.19611624976361455TruSeq Adapter, Index 6 (100% over 42bp)
GATCGGAAGAGCACACGTTTGAACTCCAGTCACGCCAATATCTCGTATGC88450.18502914444364488TruSeq Adapter, Index 6 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCTAGTCACGCCAATATCTCGTATGC87020.1820377179139172TruSeq Adapter, Index 6 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAAT85780.17944375365037712TruSeq Adapter, Index 6 (100% over 39bp)
GATCGGAAGAGCACACGTCTGAACTCCAGGCACGCCAATATCTCGTATGC85620.1791090485841139TruSeq Adapter, Index 6 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATG85490.178837100717775TruSeq Adapter, Index 6 (100% over 49bp)
GATCGGAAGAGCACATGTCTGAACTCCAGTCACGCCAATATCTCGTATGC82700.17300068112480985TruSeq Adapter, Index 6 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTTCAGTCACGCCAATATCTCGTATGC80700.16881686779651942TruSeq Adapter, Index 6 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATAACTCGTATGC79080.16542797900060416TruSeq Adapter, Index 6 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCT76350.15971707380748768TruSeq Adapter, Index 6 (100% over 43bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGCATGC74400.1556378558124045TruSeq Adapter, Index 6 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAATTCCAGTCACGCCAATATCTCGTATGC73260.15325308221527895TruSeq Adapter, Index 6 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCA68360.14300273956096737TruSeq Adapter, Index 6 (100% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATAT65540.13710356276807784TruSeq Adapter, Index 6 (100% over 41bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCC52790.11043175280022627TruSeq Adapter, Index 6 (100% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCCAGTCACGCCAATATCTCGTATG52480.10978326173434125Illumina Multiplexing PCR Primer 2.01 (96% over 27bp)
GATCGGAAGAGTACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGC51810.10838168426936395TruSeq Adapter, Index 6 (98% over 50bp)
GATCGGAAGAGCATACGTCTGAACTCCAGTCACGCCAATATCTCGTATGC48610.10168758294409924TruSeq Adapter, Index 6 (98% over 50bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)