FastQCFastQC Report
Wed 13 Sep 2023
stdin:SRR6235734

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6235734
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences472084
Total Bases81.5 Mbp
Sequences flagged as poor quality0
Sequence length61-200
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGC13237628.040772404910992TruSeq Adapter, Index 6 (100% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCCAATATCTCGTATGC22640.4795756687369197TruSeq Adapter, Index 6 (98% over 50bp)
TCTGCAGGATATCGCGGCCGCCATCTGCCCTACGTTTGAGGGTTATAAGC13860.29359181840519905No Hit
TCTGCAGGATATCGCGGCCGCGTCTTCAGAGGGGGATAGCATGACCTCAC13100.2774929885359385No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATG12480.2643597325899628TruSeq Adapter, Index 6 (100% over 49bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATAACTCGTATGC11880.2516501300615992TruSeq Adapter, Index 6 (98% over 50bp)
GATCGGAAGAGCACACGGCTGAACTCCAGTCACGCCAATATCTCGTATGC7520.15929368502215707TruSeq Adapter, Index 6 (98% over 50bp)
TCTGCAGGATATCGCGGCCGCAGAAGACCTAAGCTAGCGTTTAGAGTGCA7080.14997330983469045No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATATCGTATGC6700.14192389490006016TruSeq Adapter, Index 6 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCTAGTCACGCCAATATCTCGTATGC5160.10930258174392693TruSeq Adapter, Index 6 (98% over 50bp)
GATCGGAAGAGCACACGTTTGAACTCCAGTCACGCCAATATCTCGTATGC5100.10803162149109057TruSeq Adapter, Index 6 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGGCACGCCAATATCTCGTATGC4980.10548970098541784TruSeq Adapter, Index 6 (98% over 50bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)