FastQCFastQC Report
Wed 13 Sep 2023
stdin:SRR6235735

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6235735
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1403725
Total Bases105.5 Mbp
Sequences flagged as poor quality0
Sequence length30-200
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGC84497260.19498121070722TruSeq Adapter, Index 2 (100% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTC297562.119788420096529TruSeq Adapter, Index 2 (100% over 44bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCG190901.3599529822436731TruSeq Adapter, Index 2 (100% over 45bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCT103150.7348305401699051TruSeq Adapter, Index 2 (100% over 43bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATC96240.685604374076119TruSeq Adapter, Index 2 (100% over 42bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATG85620.609948529804627TruSeq Adapter, Index 2 (100% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTA84320.6006874565887192TruSeq Adapter, Index 2 (100% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGAATCTCGTATGC72960.5197599244866338TruSeq Adapter, Index 2 (98% over 50bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATG68710.4894833389730895TruSeq Adapter, Index 2 (100% over 49bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTAT58220.41475360202318834TruSeq Adapter, Index 2 (100% over 48bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGGATCTCGTATGC50250.3579760993072005TruSeq Adapter, Index 2 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTAT48980.3489287431655061TruSeq Adapter, Index 2 (100% over 41bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGT45280.32257030401253806TruSeq Adapter, Index 2 (100% over 46bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTA43820.3121694064008264TruSeq Adapter, Index 2 (100% over 47bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGGATGC41380.29478708436481504TruSeq Adapter, Index 2 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGA38170.27191935742399687TruSeq Adapter, Index 2 (100% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGT36510.26009367931753014TruSeq Adapter, Index 2 (100% over 39bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCG36470.2598087232185791TruSeq Adapter, Index 2 (100% over 35bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATATCGTATGC33900.24150029386097704TruSeq Adapter, Index 2 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGAT33300.23722595237671198TruSeq Adapter, Index 2 (100% over 37bp)
TCTGCAGGATATCGCGGCCGCCATCTGCCCTACGTTTGAGGGTTATAAGC32960.2348038255356284No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGCATCTCGTATGC31150.22190956205809542TruSeq Adapter, Index 2 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACC30240.21542681080696005TruSeq Adapter, Index 2 (100% over 34bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTAGCTCGTATGC28210.20096528878519654TruSeq Adapter, Index 2 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATG28020.19961174731517925TruSeq Adapter, Index 2 (100% over 49bp)
TCTGCAGGATATCGCGGCCGCGTCTTCAGAGGGGGATAGCATGACCTCAC27590.19654846925145597No Hit
GAATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATG24020.17111613742007872TruSeq Adapter, Index 2 (97% over 49bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGCC21080.1501718641471798TruSeq Adapter, Index 2 (100% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTACCTCGTATGC20380.14518513241553724TruSeq Adapter, Index 2 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCTAGTCACCGATGTATCTCGTATGC19260.13720636164490907TruSeq Adapter, Index 2 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGCATGC19030.1355678640759408TruSeq Adapter, Index 2 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTAACTCGTATGC18730.13343069333380825TruSeq Adapter, Index 2 (98% over 50bp)
GATCGGAAGAGCACATGTCTGAACTCCAGTCACCGATGTATCTCGTATGC18200.12965502502270743TruSeq Adapter, Index 2 (98% over 50bp)
GATCGGAAGAGCACACGTTTGAACTCCAGTCACCGATGTATCTCGTATGC17350.12359970791999857TruSeq Adapter, Index 2 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAATTCCAGTCACCGATGTATCTCGTATGC17270.12302979572209657TruSeq Adapter, Index 2 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATTC16060.11440987372882865TruSeq Adapter, Index 2 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCCAGTCACCGATGTATCTCGTATG15780.11241518103617162Illumina Multiplexing PCR Primer 2.01 (96% over 27bp)
GATTGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGC15570.11091916151667884TruSeq Adapter, Index 2 (98% over 50bp)
TCTGCAGGATATCGCGGCCGCAGAAGACCTAAGCTAGCGTTTAGAGTGCA14650.1043651712408057No Hit
GATCGGAAGAGCACACGTCTGAACTTCAGTCACCGATGTATCTCGTATGC14450.10294039074605069TruSeq Adapter, Index 2 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTTACCGATGTATCTCGTATGC14090.10037578585549164TruSeq Adapter, Index 2 (98% over 50bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)