FastQCFastQC Report
Wed 13 Sep 2023
stdin:SRR6235738

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6235738
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences889555
Total Bases65.5 Mbp
Sequences flagged as poor quality0
Sequence length30-200
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGC58792466.09192236567722TruSeq Adapter, Index 2 (100% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTC140931.5842752837092702TruSeq Adapter, Index 2 (100% over 44bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCG103131.1593437168022214TruSeq Adapter, Index 2 (100% over 45bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATG45450.510929622114428TruSeq Adapter, Index 2 (100% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGGATCTCGTATGC42000.4721461854522767TruSeq Adapter, Index 2 (98% over 50bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATG41120.46225359870946714TruSeq Adapter, Index 2 (100% over 49bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCT39720.4465153925277245TruSeq Adapter, Index 2 (100% over 43bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTA38650.4344869063745356TruSeq Adapter, Index 2 (100% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATC36410.40930577648374755TruSeq Adapter, Index 2 (100% over 42bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGAATCTCGTATGC34840.39165650240850763TruSeq Adapter, Index 2 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTAT33240.3736699810579447TruSeq Adapter, Index 2 (100% over 48bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGT26710.3002624907959598TruSeq Adapter, Index 2 (100% over 46bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGGATGC24860.2794655754843714TruSeq Adapter, Index 2 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTA23330.2622659644428956TruSeq Adapter, Index 2 (100% over 47bp)
TCTGCAGGATATCGCGGCCGCCATCTGCCCTACGTTTGAGGGTTATAAGC22220.2497878152559426No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCG21120.23742208182743058TruSeq Adapter, Index 2 (100% over 35bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTAT20840.23427444059108204TruSeq Adapter, Index 2 (100% over 41bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACC20180.22685500053397484TruSeq Adapter, Index 2 (100% over 34bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGA19900.22370735929762636TruSeq Adapter, Index 2 (100% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGT18880.2122409519366425TruSeq Adapter, Index 2 (100% over 39bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGAT17740.1994255554743664TruSeq Adapter, Index 2 (100% over 37bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGCC17560.19740207182242808TruSeq Adapter, Index 2 (100% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTAGCTCGTATGC17100.19223094693414122TruSeq Adapter, Index 2 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATG17010.19121920510817209TruSeq Adapter, Index 2 (100% over 49bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGCATCTCGTATGC16630.18694740628741338TruSeq Adapter, Index 2 (98% over 50bp)
TCTGCAGGATATCGCGGCCGCGTCTTCAGAGGGGGATAGCATGACCTCAC15960.17941555047186514No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTACCTCGTATGC15530.17458167285890136TruSeq Adapter, Index 2 (98% over 50bp)
GAATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATG15000.16862363766152738TruSeq Adapter, Index 2 (97% over 49bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATATCGTATGC13040.1465901490070878TruSeq Adapter, Index 2 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGCATGC11420.12837879613964287TruSeq Adapter, Index 2 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATTC11020.12388216580200212TruSeq Adapter, Index 2 (98% over 50bp)
TCTGCAGGATATCGCGGCCGCAGAAGACCTAAGCTAGCGTTTAGAGTGCA9880.11106676933972605No Hit
GATCGGAAGAGCACACGTTTGAACTCCAGTCACCGATGTATCTCGTATGC9530.10713221779429041TruSeq Adapter, Index 2 (98% over 50bp)
GATCGGAAGAGCACATGTCTGAACTCCAGTCACCGATGTATCTCGTATGC9330.10488390262547004TruSeq Adapter, Index 2 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCTAGTCACCGATGTATCTCGTATGC9300.10454665535014698TruSeq Adapter, Index 2 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCATGTATCTCGTATGC8910.10016244077094727TruSeq Adapter, Index 2 (98% over 50bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)