FastQCFastQC Report
Wed 13 Sep 2023
stdin:SRR6235739

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6235739
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7303669
Total Bases499.1 Mbp
Sequences flagged as poor quality0
Sequence length30-200
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGC423929858.0434025693114TruSeq Adapter, Index 4 (100% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATATCGTATGC1249361.7105923064147623TruSeq Adapter, Index 4 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGGATGC447150.6122265398390864TruSeq Adapter, Index 4 (98% over 50bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGCC314930.4311942394979838TruSeq Adapter, Index 4 (100% over 50bp)
GAATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATG275520.3772350581604944TruSeq Adapter, Index 4 (97% over 49bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATG265150.36303671483469474TruSeq Adapter, Index 4 (100% over 49bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAAACTCGTATGC245580.33624196277240936TruSeq Adapter, Index 4 (98% over 50bp)
GATCGGAAGAGCACATGTCTGAACTCCAGTCACTGACCAATCTCGTATGC185980.2546391409577844TruSeq Adapter, Index 4 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCTAGTCACTGACCAATCTCGTATGC176670.2418921229864059TruSeq Adapter, Index 4 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATTC174870.23942760823361517TruSeq Adapter, Index 4 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTTCAGTCACTGACCAATCTCGTATGC167080.22876173605348216TruSeq Adapter, Index 4 (98% over 50bp)
GATCGGAAGAGTACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGC164820.2256674008638672TruSeq Adapter, Index 4 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAATTCCAGTCACTGACCAATCTCGTATGC164300.2249554299352832TruSeq Adapter, Index 4 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGCATGC162530.22253199042837238TruSeq Adapter, Index 4 (98% over 50bp)
GATCGGAAGAGCACACGTTTGAACTCCAGTCACTGACCAATCTCGTATGC150790.20645787754072645TruSeq Adapter, Index 4 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAACCTCGTATGC149140.20419873901733496TruSeq Adapter, Index 4 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTTACTGACCAATCTCGTATGC144480.1978183841573324TruSeq Adapter, Index 4 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAAGCTCGTATGC136580.18700190274230663TruSeq Adapter, Index 4 (98% over 50bp)
GATCGGAAGAGCATACGTCTGAACTCCAGTCACTGACCAATCTCGTATGC128970.1765824820374527TruSeq Adapter, Index 4 (98% over 50bp)
GATTGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGC115340.15792062865937653TruSeq Adapter, Index 4 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCATCTCGTATGCC96830.13257720195151232TruSeq Adapter, Index 4 (97% over 39bp)
GGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGCCGTC90840.12437584452416998TruSeq Adapter, Index 4 (100% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATTTCGTATGC89760.12289713567249558TruSeq Adapter, Index 4 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATGTCGTATGC85730.11737936097596974TruSeq Adapter, Index 4 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGG78160.10701470726562225TruSeq Adapter, Index 4 (97% over 49bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGACCAATCTCGTATGC74160.1015380078149763TruSeq Adapter, Index 4 (98% over 50bp)

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)