##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	SRR6235741
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	171298
Total Bases	10 Mbp
Sequences flagged as poor quality	0
Sequence length	30-200
%GC	39
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	26.543234597018063	28.0	16.0	34.0	10.0	41.0
2	26.32876040584245	30.0	16.0	34.0	10.0	41.0
3	26.231106025756283	30.0	16.0	34.0	10.0	41.0
4	28.29832805987227	33.0	19.0	37.0	10.0	41.0
5	28.188058237691042	32.0	19.0	37.0	10.0	41.0
6	27.886752910133218	32.0	17.0	37.0	10.0	41.0
7	27.866419923174817	32.0	17.0	37.0	10.0	41.0
8	27.731275321369775	32.0	17.0	37.0	10.0	41.0
9	28.259109855339815	32.0	17.0	39.0	10.0	41.0
10-14	28.099772326588756	32.0	17.0	39.4	10.0	41.0
15-19	27.9434365841983	32.0	17.2	41.0	10.0	41.0
20-24	27.972271713621875	32.0	17.2	41.0	10.0	41.0
25-29	28.22931382736518	32.0	17.0	41.0	9.8	41.0
30-34	29.61499085954508	33.0	21.2	41.0	9.0	41.0
35-39	31.017898417638737	34.6	24.6	41.0	9.0	41.0
40-44	32.46703179418414	37.2	27.8	41.0	12.4	41.0
45-49	33.9663999087441	39.6	30.2	41.0	15.4	41.0
50-54	35.091378447936584	40.6	32.2	41.0	20.0	41.0
55-59	36.19906140162669	41.0	34.6	41.0	24.0	41.0
60-64	37.00610531528867	41.0	36.0	41.0	27.6	41.0
65-69	37.339227148518795	41.0	36.4	41.0	29.0	41.0
70-74	37.47052770785884	41.0	36.0	41.0	30.0	41.0
75-79	37.318042497949065	41.0	35.2	41.0	29.8	41.0
80-84	37.329047966859946	41.0	35.0	41.0	30.4	41.0
85-89	37.16061895165622	41.0	35.0	41.0	30.8	41.0
90-94	36.97259519700352	41.0	35.0	41.0	30.8	41.0
95-99	36.76294187077508	41.0	35.0	41.0	31.0	41.0
100-104	35.4980943779072	37.0	33.6	40.2	30.2	41.0
105-109	35.9489865545831	37.4	35.0	40.0	30.0	41.0
110-114	36.454887834938816	39.0	35.0	40.2	30.2	41.0
115-119	36.832436446302914	39.0	35.0	41.0	30.6	41.0
120-124	36.87842771200955	39.0	35.0	41.0	31.0	41.0
125-129	36.8342915105407	39.0	35.0	41.0	30.8	41.0
130-134	36.88032832656128	39.0	35.2	41.0	30.4	41.0
135-139	36.952345139255655	39.0	35.0	41.0	31.0	41.0
140-144	36.83532712853546	39.0	35.0	41.0	30.4	41.0
145-149	36.70514297620986	39.0	35.0	40.6	30.0	41.0
150-154	36.336107875264204	38.4	34.6	40.0	30.0	41.0
155-159	36.549559660415426	38.6	35.0	40.6	30.0	41.0
160-164	36.71996118077449	38.6	35.0	41.0	30.8	41.0
165-169	36.12580995189085	37.6	34.0	40.0	29.6	41.0
170-174	35.64948397969249	37.0	34.0	39.4	29.0	41.0
175-179	35.19922043664512	36.2	34.0	39.0	29.4	40.4
180-184	34.54346483667926	35.2	33.8	37.2	29.0	39.2
185-189	33.978581588080274	35.0	33.6	36.4	29.0	37.8
190-194	33.63079305939145	35.0	33.6	35.6	29.0	36.6
195-199	33.57743381234476	35.0	33.8	35.0	29.6	36.0
200	33.71832517672648	35.0	34.0	35.0	30.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
6	1.0
7	0.0
8	1.0
9	2.0
10	3.0
11	7.0
12	13.0
13	16.0
14	25.0
15	38.0
16	60.0
17	524.0
18	5759.0
19	16198.0
20	19448.0
21	17109.0
22	13410.0
23	9905.0
24	7452.0
25	5579.0
26	4331.0
27	3558.0
28	2906.0
29	2441.0
30	2019.0
31	1700.0
32	1526.0
33	1702.0
34	1744.0
35	2034.0
36	2884.0
37	3489.0
38	5112.0
39	6964.0
40	7947.0
41	25391.0
>>END_MODULE
>>Per base sequence content	pass
#Base	G	A	T	C
1	22.158460694228772	30.85850389379911	26.876554308865252	20.106481103106866
2	23.737877965191295	29.8092585781094	27.915856584870298	18.537006871829004
3	20.50933171381187	29.70498236434561	27.976454649505968	21.80923127233655
4	20.42216512904356	29.755926661216865	29.737241620927247	20.084666588812333
5	20.152286635212782	29.4453916943056	29.053580604475172	21.348741066006447
6	19.93600112114824	30.045021109119258	29.535832948911846	20.483144820820655
7	19.833576642335764	30.122627737226278	29.58948905109489	20.454306569343068
8	20.00478806953211	29.98791304398601	29.081683298396	20.925615588085883
9	19.698402690205967	30.60050908411564	28.285694269300826	21.415393956377564
10-14	19.791097473834643	30.57937408637253	29.44974523512626	20.179783204666563
15-19	19.5746091943823	30.83067503000808	29.82709741299992	19.7676183626097
20-24	19.10032985959074	31.15380798085063	30.265347228303703	19.480514931254923
25-29	18.660674251765496	31.83114280910212	30.75070993535852	18.757473003773868
30-34	18.694774244546885	31.919606170414255	30.90150868872175	18.48411089631711
35-39	18.874380196581594	31.691768597544712	30.64707390827684	18.786777297596856
40-44	18.88851839502056	31.069467600311214	30.614204734911638	19.427809269756587
45-49	19.24369490097864	30.538349337208103	30.514085547985133	19.70387021382813
50-54	19.423410976452203	29.88444074821765	30.38946754213587	20.30268073319428
55-59	19.926466641618806	29.44056373018855	30.272893871197777	20.36007575699487
60-64	20.305878017320804	29.65748346981715	29.671196129601775	20.36544238326027
65-69	20.420901432142166	29.845823824306255	29.21409367750105	20.51918106605053
70-74	20.676652945849195	29.494599362446227	29.265575191111388	20.563172500593193
75-79	20.930607077011928	28.876696505919725	29.67469290743908	20.518003509629267
80-84	20.71926679807767	29.054734071378803	29.477551944068935	20.748447186474593
85-89	20.90664485163006	29.032319914103294	29.622859772348164	20.438175461918487
90-94	20.528490096805356	29.59311606365997	29.454724827524785	20.423669012009892
95-99	20.435354834893456	29.62157845703385	29.28145749227334	20.66160921579936
100-104	20.380047505938244	29.387982008389347	29.828675392934755	20.403295092737654
105-109	20.433538216112233	29.396377797588325	29.485525266269413	20.684558720030026
110-114	20.48709329612482	29.52495718906577	29.58330690683072	20.40464260797869
115-119	20.354904622810988	29.548415408455725	29.559386185049576	20.53729378368371
120-124	20.36696431220149	29.535138983654218	29.49064048177045	20.607256222373845
125-129	20.21208432113795	29.58127763183187	29.646953290190297	20.55968475683988
130-134	20.053713747580264	29.64545438691338	29.51640187649326	20.7844299890131
135-139	20.618986286839913	29.701113720736423	29.627244488218807	20.052655504204864
140-144	20.41774377003412	29.560541826077962	29.65360355702616	20.36811084686175
145-149	20.577266460230998	29.65666885156974	29.880433062857403	19.885631625341862
150-154	20.337230769230768	29.944615384615386	29.64676923076923	20.071384615384616
155-159	20.312458129002867	29.855025859527828	30.093523059195544	19.73899295227376
160-164	20.28197028197028	29.887679887679887	29.93155493155493	19.8987948987949
165-169	20.764957127717654	29.586691929734414	30.097305629596327	19.551045312951604
170-174	20.09679242616928	29.896142433234424	30.281192595732655	19.72587254486364
175-179	20.456917546097646	29.672581081603468	29.896787660906877	19.973713711392012
180-184	20.517395182872438	29.658892995199864	29.463489231553456	20.360222590374242
185-189	19.789809514872854	29.77385755841231	30.519846110537674	19.916486816177162
190-194	19.58929745586971	30.55991098072935	29.927671842597743	19.923119720803196
195-199	19.13294797687861	30.520231213872833	30.19968470835523	20.147136100893327
200	19.73898858075041	29.690048939641112	32.1642196846112	18.40674279499728
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1815.0
1	1492.0
2	885.0
3	613.5
4	728.5
5	957.5
6	1124.5
7	1264.0
8	1570.5
9	1874.5
10	2091.5
11	2426.5
12	2739.833333333333
13	2962.5
14	3227.6666666666665
15	3553.5
16	3889.3333333333335
17	4184.833333333333
18	4443.166666666666
19	4642.0
20	4942.666666666667
21	5330.666666666667
22	5721.0
23	6109.166666666666
24	6371.666666666666
25	6505.833333333334
26	6651.666666666667
27	6966.833333333334
28	7302.0
29	7516.333333333333
30	7699.666666666666
31	7899.666666666666
32	8016.833333333333
33	8097.0
34	8321.166666666666
35	8703.166666666666
36	9117.833333333332
37	9406.5
38	9570.0
39	9644.333333333332
40	9622.333333333332
41	9561.333333333332
42	9355.0
43	9243.333333333332
44	9120.333333333332
45	8757.166666666666
46	8465.333333333332
47	8201.333333333332
48	7875.833333333333
49	7590.5
50	7224.833333333333
51	6763.666666666666
52	6322.166666666666
53	5944.166666666666
54	5581.666666666666
55	5266.833333333334
56	5010.166666666667
57	4695.833333333334
58	4358.833333333334
59	4124.0
60	3878.0
61	3535.8333333333335
62	3259.3333333333335
63	2992.166666666667
64	2709.333333333333
65	2522.6666666666665
66	2292.1666666666665
67	2015.6666666666665
68	1763.3333333333333
69	1538.1666666666667
70	1314.3333333333335
71	1134.3333333333335
72	977.3333333333334
73	803.5
74	691.0
75	618.5
76	527.5
77	447.0
78	392.5
79	359.0
80	321.0
81	279.0
82	263.0
83	239.0
84	215.0
85	189.5
86	171.5
87	174.0
88	163.5
89	126.0
90	97.0
91	98.5
92	95.0
93	84.5
94	78.0
95	67.5
96	50.0
97	43.0
98	41.0
99	30.5
100	25.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.011091781573631916
3	0.031524010788217025
4	0.022183563147263833
5	0.024518675057502128
6	0.026270008990180855
7	0.028021342922859578
8	0.02276734112482341
9	0.005837779775595745
10-14	0.0016345783371668088
15-19	0.006304802157643405
20-24	0.007589113708274468
25-29	0.008639914067881704
30-34	0.0052173958412137745
35-39	0.004990878738856572
40-44	0.00533482708491711
45-49	0.01059685384986489
50-54	0.02715541951770634
55-59	0.034109679867240526
60-64	0.016709511568123392
65-69	0.028814087727088076
70-74	0.026815458080219474
75-79	0.011660383015819254
80-84	0.009527382292169087
85-89	0.003195459890587453
90-94	0.0054805406553356485
95-99	0.002957486136783734
100-104	0.01010662489261711
105-109	0.008210276920911575
110-114	0.011414945969255744
115-119	0.012340600575894693
120-124	0.004449652185520832
125-129	0.014414529846084856
130-134	0.01743648759393908
135-139	0.015150367396409362
140-144	0.01033912324234905
145-149	0.0045202847779410105
150-154	0.00738407009943881
155-159	0.029468495499357053
160-164	0.017546938059308653
165-169	0.0
170-174	0.0
175-179	0.02318840579710145
180-184	0.008495094083166971
185-189	0.0
190-194	0.0
195-199	0.0
200	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
30-34	42899.0
35-39	29360.0
40-44	21134.0
45-49	14551.0
50-54	8555.0
55-59	6144.0
60-64	4736.0
65-69	3861.0
70-74	3044.0
75-79	2452.0
80-84	2372.0
85-89	2170.0
90-94	2108.0
95-99	2102.0
100-104	8226.0
105-109	1311.0
110-114	1211.0
115-119	1141.0
120-124	1052.0
125-129	1003.0
130-134	939.0
135-139	910.0
140-144	850.0
145-149	762.0
150-154	680.0
155-159	632.0
160-164	623.0
165-169	593.0
170-174	523.0
175-179	468.0
180-184	447.0
185-189	438.0
190-194	126.0
195-199	197.0
200-201	3678.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	96.38683436369041
#Duplication Level	Percentage of total
1	95.22172874427885
2	1.7232729015896069
3	0.32173364698548257
4	0.20969887851051092
5	0.1362268683835944
6	0.10440012871308257
7	0.12147699619785288
8	0.10008549905408003
9	0.14724116687763694
>10	0.8093452375430894
>50	0.5774163406966155
>100	0.5273735911695754
>500	0.0
>1k	0.0
>5k	0.0
>10k+	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	0.0052540017980361705	0.0	0.0	0.0	0.9480554355567491	0.015762005394108512
2	0.00642155775315532	0.0	0.0	0.0	1.0151899029761002	0.016345783371668088
3	0.008172891685834044	0.0	0.0	0.0	1.072983922754498	0.02043222921458511
4	0.008172891685834044	0.0	0.0	0.0	1.147123725904564	0.02276734112482341
5	0.009924225618512767	0.0	0.0	0.0	1.2136744153463555	0.027437564945300003
6	0.009924225618512767	0.0	0.0	0.0	1.2878142184964214	0.030356454833097877
7	0.009924225618512767	0.0	0.0	0.0	1.3421055704094618	0.03619423460869362
8	0.011091781573631916	0.0	0.0	0.0	1.402234702098098	0.041448236406729795
9	0.011091781573631916	0.0	0.0	0.0	1.476374505248164	0.04786979415988511
10-14	0.015762005394108512	0.0	0.0	0.0	1.8118133311538955	0.06211397681233873
15-19	0.02545271982159745	0.0	0.0	0.0	2.7039428364604374	0.08791696342047192
20-24	0.048803838923980436	0.0	0.0	0.0	3.857604875713668	0.10928323739915236
25-29	0.1716307254025149	0.0	0.0	0.0	4.714707702366636	0.12796413268105872
30-34	0.34676411867038726	0.0	0.0	0.0	5.253768286845147	0.13788835829957152
35-39	0.395217690807832	0.0	0.0	0.0	5.536900605961541	0.14185804854697662
40-44	0.4317621922030613	0.0	0.0	0.0	5.676540298193791	0.14430991605272683
45-49	0.4671391376431716	0.0	0.0	0.0	5.743908276804166	0.14722880594052468
50-54	0.49702857009422174	0.0	0.0	0.0	5.774264731637263	0.1487466286821796
55-59	0.5246996462305455	0.0	0.0	0.0	5.7921283377505866	0.1497974290417868
60-64	0.5496853436700955	0.0	0.0	0.0	5.804154164088314	0.15049796261485832
65-69	0.5714018844353115	0.0	0.0	0.0	5.814195145302339	0.15061471821037023
70-74	0.5910168244813134	0.0	0.0	0.0	5.818748613527303	0.15061471821037023
75-79	0.6088804305946363	0.0	0.0	0.0	5.821433992224078	0.15061471821037023
80-84	0.6213732793144111	0.0	0.0	0.0	5.824703148898411	0.15108174059241788
85-89	0.6335158612476504	0.0	0.0	0.0	5.827622038786208	0.1511984961879298
90-94	0.6422725309110439	0.0	0.0	0.0	5.831708484629125	0.1511984961879298
95-99	0.650445422596878	0.0	0.0	0.0	5.834043596539365	0.15166551856997748
100-104	0.6590853366647597	0.0	0.0	0.0	5.837779775595746	0.1517822741654894
105-109	0.6685425399012248	0.0	0.0	0.0	5.8399981319104715	0.1517822741654894
110-114	0.6737965416992608	0.0	0.0	0.0	5.8418662214386625	0.15224929654753708
115-119	0.6796343214748568	0.0	0.0	0.0	5.842449999416222	0.15236605214304896
120-124	0.6837207673177736	0.0	0.0	0.0	5.842800266202758	0.15283307452509662
125-129	0.6872234351831312	0.0	0.0	0.0	5.8430337773937815	0.15294983012060853
130-134	0.6909596142395124	0.0	0.0	0.0	5.844434844539924	0.15294983012060853
135-139	0.6955130824644773	0.0	0.0	0.0	5.846302934068116	0.15294983012060853
140-144	0.697030905206132	0.0	0.0	0.0	5.846653200854651	0.15294983012060853
145-149	0.7007670842625133	0.0	0.0	0.0	5.847120223236699	0.15294983012060853
150-154	0.7045032633188946	0.0	0.0	0.0	5.847470490023234	0.15294983012060853
155-159	0.7091734871393711	0.0	0.0	0.0	5.847704001214258	0.15294983012060853
160-164	0.7125593994092168	0.0	0.0	0.0	5.847937512405283	0.15294983012060853
165-169	0.7147777557239431	0.0	0.0	0.0	5.848287779191818	0.15294983012060853
170-174	0.7167626008476458	0.0	0.0	0.0	5.848521290382842	0.15294983012060853
175-179	0.7182804235893004	0.0	0.0	0.0	5.848871557169377	0.1534168525026562
180-184	0.719097712757884	0.0	0.0	0.0	5.848871557169377	0.1535336080981681
185-189	0.7192144683533959	0.0	0.0	0.0	5.848871557169377	0.1535336080981681
>>END_MODULE
