FastQCFastQC Report
Wed 13 Sep 2023
stdin:SRR6235742

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6235742
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences985309
Total Bases69.1 Mbp
Sequences flagged as poor quality0
Sequence length30-200
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGC55549856.37804993154432TruSeq Adapter, Index 4 (100% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTC317533.2226438609613837TruSeq Adapter, Index 4 (100% over 44bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCG198592.015509855283977TruSeq Adapter, Index 4 (100% over 45bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAA115991.1771941593956818TruSeq Adapter, Index 4 (100% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATATCGTATGC98470.99938191978354TruSeq Adapter, Index 4 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTAT95280.9670062893975392TruSeq Adapter, Index 4 (100% over 48bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTA77540.7869612476898109TruSeq Adapter, Index 4 (100% over 47bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGT71460.7252547170481545TruSeq Adapter, Index 4 (100% over 46bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCA67820.6883119914666364TruSeq Adapter, Index 4 (100% over 39bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATG53040.5383082870449777TruSeq Adapter, Index 4 (100% over 49bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGCC52420.5320158447755984TruSeq Adapter, Index 4 (100% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGGATGC51300.5206488522889774TruSeq Adapter, Index 4 (98% over 50bp)
GAATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATG39710.40302077825331956TruSeq Adapter, Index 4 (97% over 49bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAAT37050.37602417109759473TruSeq Adapter, Index 4 (100% over 41bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATG33000.3349203143379387TruSeq Adapter, Index 4 (100% over 49bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACC26580.2697630895485579TruSeq Adapter, Index 4 (100% over 38bp)
GATCGGAAGAGCACATGTCTGAACTCCAGTCACTGACCAATCTCGTATGC25420.2579901330445576TruSeq Adapter, Index 4 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCT25090.2546409299011782TruSeq Adapter, Index 4 (100% over 43bp)
GATCGGAAGAGCACACGTCTGAATTCCAGTCACTGACCAATCTCGTATGC22750.230892034884488TruSeq Adapter, Index 4 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAAACTCGTATGC22630.22967414283235005TruSeq Adapter, Index 4 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAACCTCGTATGC22160.22490406562814305TruSeq Adapter, Index 4 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATTC21970.22297573654559127TruSeq Adapter, Index 4 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTTCAGTCACTGACCAATCTCGTATGC21480.21800267733269463TruSeq Adapter, Index 4 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCTAGTCACTGACCAATCTCGTATGC21250.21566838423276352TruSeq Adapter, Index 4 (98% over 50bp)
GATCGGAAGAGCACACGTTTGAACTCCAGTCACTGACCAATCTCGTATGC20110.2040984097374529TruSeq Adapter, Index 4 (98% over 50bp)
GATCGGAAGAGTACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGC19890.20186560764186667TruSeq Adapter, Index 4 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCACCAATCTCGTATGC18190.1846121369032456TruSeq Adapter, Index 4 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTTACTGACCAATCTCGTATGC18170.18440915489455592TruSeq Adapter, Index 4 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATC17890.18156740677290067TruSeq Adapter, Index 4 (100% over 42bp)
GATTGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGC17220.17476750948179706TruSeq Adapter, Index 4 (98% over 50bp)
GATCGGAAGAGCATACGTCTGAACTCCAGTCACTGACCAATCTCGTATGC17060.1731436534122798TruSeq Adapter, Index 4 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGCATGC16290.16532884607772788TruSeq Adapter, Index 4 (98% over 50bp)
GGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGCCGTC15260.15487527263021042TruSeq Adapter, Index 4 (100% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAAGCTCGTATGC15160.1538603625867621TruSeq Adapter, Index 4 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGAC14900.15122159647379654TruSeq Adapter, Index 4 (100% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCATCTCGTATGCC12860.13051743158745124TruSeq Adapter, Index 4 (97% over 39bp)
CATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGC11420.11590272696179575TruSeq Adapter, Index 4 (98% over 50bp)
AGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGCCGTCTTC11410.11580123595745093TruSeq Adapter, Index 4 (100% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGG11000.11164010477931288TruSeq Adapter, Index 4 (97% over 49bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATTTCGTATGC10490.10646406355772657TruSeq Adapter, Index 4 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATGTCGTATGC10260.10412977045779546TruSeq Adapter, Index 4 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGC10230.10382529744476097TruSeq Adapter, Index 5 (100% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCAGTCACTGACCAATCTCGTATGCC10050.10199845936655404Illumina Multiplexing PCR Primer 2.01 (96% over 26bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)