FastQCFastQC Report
Wed 13 Sep 2023
stdin:SRR6235837

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6235837
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4035860
Total Bases457.5 Mbp
Sequences flagged as poor quality0
Sequence length30-200
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCGGAAGAGCACACGTCTGAACTCCAGTCACGCAACGATCTCGTATGC610591.5129117461953585TruSeq Adapter, Index 6 (97% over 35bp)
CAGGCGGAAGAGCACACGTCTGAACTCCAGTCACGCAACGATCTCGTATG230670.5715510448826272TruSeq Adapter, Index 6 (96% over 33bp)
GACCGGAAGAGCACACGTCTGAACTCCAGTCACGCAACGATCTCGTATGC175740.43544622459649246TruSeq Adapter, Index 6 (97% over 35bp)
CAGACGGAAGAGCACACGTCTGAACTCCAGTCACGCAACGATCTCGTATG155620.3855931573444074TruSeq Adapter, Index 6 (96% over 33bp)
GGCGGAAGAGCACACGTCTGAACTCCAGTCACGCAACGATCTCGTATGCC144160.357197722418518TruSeq Adapter, Index 6 (97% over 34bp)
GAAGAGCACACGTCTGAACTCCAGTCACGCAACGATCTCGTATGCCGTCT143130.3546456021764878TruSeq Adapter, Index 6 (96% over 32bp)
AGACGGAAGAGCACACGTCTGAACTCCAGTCACGCAACGATCTCGTATGC130020.3221618192900646TruSeq Adapter, Index 6 (96% over 33bp)
GAGGCGGAAGAGCACACGTCTGAACTCCAGTCACGCAACGATCTCGTATG127580.31611601988176TruSeq Adapter, Index 6 (96% over 33bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCAACGATCTCGTATGC117100.2901488158657634TruSeq Adapter, Index 6 (97% over 37bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACGCAACGATCTCGTATGCCGT109670.2717388611101475TruSeq Adapter, Index 6 (97% over 34bp)
GACGGAAGAGCACACGTCTGAACTCCAGTCACGCAACGATCTCGTATGCC106820.26467716917831635TruSeq Adapter, Index 6 (97% over 34bp)
GGAGCGGAAGAGCACACGTCTGAACTCCAGTCACGCAACGATCTCGTATG92310.22872448499204628TruSeq Adapter, Index 6 (97% over 35bp)
GAGACGGAAGAGCACACGTCTGAACTCCAGTCACGCAACGATCTCGTATG84540.20947208277789617TruSeq Adapter, Index 6 (96% over 33bp)
AGGCGGAAGAGCACACGTCTGAACTCCAGTCACGCAACGATCTCGTATGC72090.17862363907568646TruSeq Adapter, Index 6 (96% over 33bp)
AAGACGGAAGAGCACACGTCTGAACTCCAGTCACGCAACGATCTCGTATG57020.14128339436947762TruSeq Adapter, Index 6 (96% over 33bp)
GGAAGAGCACACGTCTGAACTCCAGTCACGCAACGATCTCGTATGCCGTC52870.1310005798020744TruSeq Adapter, Index 6 (96% over 33bp)
AGAGCGGAAGAGCACACGTCTGAACTCCAGTCACGCAACGATCTCGTATG51550.12772990143364735TruSeq Adapter, Index 6 (97% over 35bp)
AGGGCGGAAGAGCACACGTCTGAACTCCAGTCACGCAACGATCTCGTATG46550.11534096821990852TruSeq Adapter, Index 6 (97% over 34bp)
CAGCCGGAAGAGCACACGTCTGAACTCCAGTCACGCAACGATCTCGTATG45990.11395340769996976TruSeq Adapter, Index 6 (96% over 33bp)
AAGGCGGAAGAGCACACGTCTGAACTCCAGTCACGCAACGATCTCGTATG43750.10840316562021478TruSeq Adapter, Index 6 (96% over 33bp)
GGGCGGAAGAGCACACGTCTGAACTCCAGTCACGCAACGATCTCGTATGC41840.10367059313256655TruSeq Adapter, Index 6 (97% over 34bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)