##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	SRR6235895
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1208890
Total Bases	128.2 Mbp
Sequences flagged as poor quality	0
Sequence length	30-200
%GC	41
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	34.7541877259304	34.0	31.0	41.0	30.0	41.0
2	34.92571946165491	34.0	31.0	41.0	30.0	41.0
3	35.00310780964356	34.0	31.0	41.0	30.0	41.0
4	37.06957456840573	37.0	37.0	41.0	33.0	41.0
5	37.002912589234754	37.0	37.0	41.0	33.0	41.0
6	36.90215486934295	37.0	37.0	41.0	33.0	41.0
7	36.88211086203046	37.0	37.0	41.0	33.0	41.0
8	36.843455566676866	37.0	37.0	41.0	33.0	41.0
9	37.68173200208456	39.0	39.0	41.0	33.0	41.0
10-14	37.702751449676974	39.4	39.2	41.0	32.2	41.0
15-19	38.08074382284575	41.0	40.0	41.0	32.0	41.0
20-24	38.07425439866324	41.0	40.0	41.0	32.0	41.0
25-29	38.20830083795879	41.0	40.0	41.0	32.0	41.0
30-34	38.54931113611771	41.0	40.0	41.0	33.0	41.0
35-39	38.879899944272516	41.0	40.0	41.0	34.4	41.0
40-44	39.13235477867985	41.0	40.0	41.0	35.2	41.0
45-49	39.38263496014032	41.0	40.0	41.0	36.0	41.0
50-54	39.42250823413972	41.0	40.0	41.0	35.2	41.0
55-59	39.37416972316171	41.0	40.0	41.0	35.0	41.0
60-64	39.204219744357566	41.0	40.0	41.0	35.0	41.0
65-69	39.00676110460186	41.0	39.0	41.0	34.6	41.0
70-74	38.77479557979564	41.0	38.0	41.0	34.0	41.0
75-79	38.471834695302604	41.0	36.6	41.0	33.4	41.0
80-84	38.53919784938704	41.0	36.0	41.0	34.0	41.0
85-89	38.41264744853282	41.0	35.6	41.0	34.0	41.0
90-94	38.24311450324535	41.0	35.0	41.0	33.2	41.0
95-99	38.00899156923869	41.0	35.0	41.0	33.0	41.0
100-104	35.725603478201144	36.6	33.8	39.4	30.8	41.0
105-109	36.13871743230387	37.4	35.0	39.0	31.0	41.0
110-114	36.681937306634644	39.0	35.2	39.4	31.0	41.0
115-119	37.092110946235664	39.0	36.0	41.0	31.0	41.0
120-124	37.06416332081249	39.0	36.0	41.0	31.0	41.0
125-129	36.95941172720115	39.0	36.0	41.0	31.0	41.0
130-134	36.928273040918086	39.0	36.0	40.4	31.0	41.0
135-139	36.84064857298292	39.0	35.0	40.0	31.0	41.0
140-144	36.8457952066681	39.0	35.0	40.4	31.0	41.0
145-149	36.5202838236775	38.4	35.0	40.2	30.2	41.0
150-154	36.173087673383634	37.8	34.6	39.8	29.8	41.0
155-159	36.486435708368745	38.0	35.0	40.0	30.6	41.0
160-164	36.20189229352674	37.4	34.4	40.0	30.4	41.0
165-169	35.692999959856614	36.8	34.0	39.4	30.0	41.0
170-174	34.66560969737301	35.4	33.2	38.4	28.6	40.4
175-179	33.813344509155044	35.0	32.8	37.0	27.2	39.0
180-184	33.32997451599564	35.0	32.2	36.0	27.2	38.0
185-189	32.81707416674106	34.4	32.0	35.0	27.0	36.8
190-194	32.78739510543845	34.8	32.0	35.0	27.0	36.0
195-199	32.745201924165066	34.0	32.0	35.0	27.4	35.6
200	33.0922539991736	34.0	32.0	35.0	29.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
6	1.0
7	1.0
8	1.0
9	3.0
10	5.0
11	3.0
12	11.0
13	23.0
14	140.0
15	567.0
16	1403.0
17	2746.0
18	6410.0
19	12364.0
20	14722.0
21	14213.0
22	12882.0
23	11864.0
24	11013.0
25	10413.0
26	10296.0
27	10160.0
28	9827.0
29	9820.0
30	10322.0
31	11428.0
32	13368.0
33	16346.0
34	21196.0
35	29907.0
36	47415.0
37	74453.0
38	92350.0
39	159846.0
40	187185.0
41	406186.0
>>END_MODULE
>>Per base sequence content	pass
#Base	G	A	T	C
1	22.530935429141966	29.173977097980046	27.481762179985093	20.813325292892895
2	22.71242614124293	29.089719258127978	28.924090323199795	19.273764277429294
3	20.750886404491776	28.729933821752418	28.879405951738548	21.63977382201726
4	20.8944025308279	28.62121224668947	29.57085241530091	20.91353280718172
5	21.204845429923786	28.61251059451049	28.82096250765332	21.36168146791241
6	21.518158117600173	28.61161388884276	28.33754006077614	21.532687932780924
7	21.186582366294047	28.949292510703213	28.58429773857957	21.279827384423175
8	21.198341603116987	28.78434180292384	28.808485131039994	21.20883146291918
9	20.985404347793494	28.774499963767	28.698618897967037	21.54147679047247
10-14	20.998694832503503	28.901442351768473	28.937513647584773	21.162349168143248
15-19	21.109645007232704	29.017185760807873	28.720408099295803	21.152761132663617
20-24	21.06635949339668	29.101433462339237	28.658913908367325	21.173293135896763
25-29	21.03954968315272	29.387831297007644	28.537057751417834	21.0355612684218
30-34	20.990496713760308	29.426819134586303	28.617964208331575	20.96471994332181
35-39	21.078539565045425	29.53651803509148	28.62112958626102	20.763812813602073
40-44	20.97677982347189	29.39335820018626	28.71051351248991	20.919348463851943
45-49	21.030205568914276	29.220475624473714	28.792010303271653	20.957308503340354
50-54	21.000604361150756	29.2228369502541	28.775386950842492	21.00117173775265
55-59	20.97313725490196	29.260457516339873	28.81666666666667	20.949738562091504
60-64	20.898274534437927	29.139977492942183	28.920572203194407	21.04117576942548
65-69	20.954160826944733	29.18673561175476	28.94554261229664	20.913560949003866
70-74	20.962655548542173	29.120134630666684	28.953766497721855	20.96344332306929
75-79	20.98819648195751	29.063132115278776	28.936535304241634	21.012136098522078
80-84	20.92127074506317	28.986322126124804	29.065293123506645	21.027114005305382
85-89	20.890507210268368	29.06072666209682	29.066001741910764	20.982764385724046
90-94	20.90449203945782	29.241210440325993	28.93942593641736	20.914871583798817
95-99	20.88213453463263	29.303162843286024	28.91050586120866	20.90419676087269
100-104	20.989767466541174	29.212240499271946	29.05922902184018	20.738763012346695
105-109	20.92259215981244	29.00265862352865	29.034782253828347	21.039966962830565
110-114	20.832234110409335	29.118564472558024	29.12019589843618	20.929005518596462
115-119	20.895353661944398	29.107906480485717	29.064254443531794	20.932485414038087
120-124	20.92444760550452	29.150850710764658	29.01686167809868	20.907840005632142
125-129	21.007773570246734	29.100402872302638	29.00931199396969	20.882511563480943
130-134	20.936179526118607	29.1501271371481	29.008020531900947	20.90567280483235
135-139	21.038409102011272	29.072834741829904	28.994730905360576	20.894025250798247
140-144	21.088086030093557	29.136668067581066	28.924498286320997	20.85074761600438
145-149	21.04098581024842	29.246851646853194	28.882066266120543	20.830096276777844
150-154	21.146425172524783	29.143219030524392	28.916731375769995	20.793624421180837
155-159	21.16690187866666	29.255540876190363	28.916805553156284	20.660751691986697
160-164	21.017253078233587	29.246270077470438	29.04083754639521	20.695639297900765
165-169	21.212583914238454	29.147256580306124	28.95537695432135	20.684782551134067
170-174	21.09071009922092	29.2245215362682	29.03135995545379	20.65340840905709
175-179	21.06038845396575	29.13383852890948	29.09540049196509	20.710372525159677
180-184	21.02556084823945	29.09403808449984	29.141512470014675	20.73888859724603
185-189	20.738266901268027	29.183414202244602	29.406645004082115	20.671673892405263
190-194	20.567427436158354	29.24224558909833	29.38384670467822	20.806480270065094
195-199	20.2697411048821	29.525452855170858	29.586866495760205	20.617939544186832
200	19.868219113393543	29.776872675757037	29.779824095389884	20.575084115459536
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	142.0
1	182.5
2	274.5
3	340.0
4	474.5
5	699.5
6	846.0
7	1035.0
8	1380.6666666666665
9	1746.3333333333333
10	2145.0
11	2704.3333333333335
12	3306.833333333333
13	3892.833333333333
14	4547.166666666667
15	5428.5
16	6377.0
17	7327.666666666666
18	8509.166666666668
19	9907.5
20	11392.333333333328
21	13077.333333333328
22	15063.000000000007
23	17205.833333333358
24	19473.500000000022
25	21603.166666666657
26	23619.666666666617
27	25559.999999999924
28	27458.4999999999
29	29375.16666666654
30	31054.666666666544
31	32547.49999999989
32	34119.83333333324
33	35653.666666666584
34	37636.16666666662
35	39953.833333333314
36	42390.666666666686
37	45158.16666666676
38	48143.166666666846
39	50764.66666666692
40	52804.83333333368
41	54346.00000000041
42	55787.16666666709
43	56965.500000000335
44	57558.00000000022
45	57268.83333333357
46	56528.3333333336
47	54925.833333333634
48	52656.5000000004
49	50319.50000000034
50	47438.16666666692
51	44428.16666666684
52	41222.16666666676
53	37841.1666666667
54	34658.66666666665
55	31619.666666666602
56	28632.499999999935
57	25883.666666666628
58	23314.166666666653
59	21029.333333333336
60	18896.00000000002
61	16829.00000000001
62	14981.33333333334
63	13299.333333333334
64	11737.499999999996
65	10360.833333333332
66	8953.999999999998
67	7715.666666666666
68	6708.333333333334
69	5756.0
70	4893.0
71	4189.333333333333
72	3549.0
73	2895.333333333333
74	2377.8333333333335
75	1974.1666666666665
76	1687.5
77	1424.0
78	1170.1666666666665
79	991.0
80	844.5
81	725.8333333333333
82	646.3333333333333
83	559.0
84	482.5
85	414.0
86	355.0
87	338.0
88	327.5
89	286.0
90	244.0
91	233.5
92	230.0
93	209.0
94	179.0
95	137.5
96	97.5
97	81.5
98	66.0
99	47.0
100	42.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	8.272051220541157E-5
2	0.013566164001687498
3	0.052775686787052585
4	0.11415430684346796
5	0.15692081165366575
6	0.36976068955818975
7	0.5526557420443547
8	0.6394295593478314
9	0.6888137051344622
10-14	0.7481243123857423
15-19	0.7725103193838977
20-24	0.4107735195096328
25-29	0.03224445565766943
30-34	0.009330627280843693
35-39	0.07304620663397539
40-44	0.2034403831098739
45-49	0.10943849307689178
50-54	0.005379281361630595
55-59	0.010783150836674476
60-64	0.04290181148028918
65-69	0.04965768969131355
70-74	0.02968044058965142
75-79	0.0021879815972729
80-84	0.0011601344352991675
85-89	0.0014463719319898901
90-94	0.0027219389777174896
95-99	0.003323467383016322
100-104	0.026594510153204617
105-109	0.04026935558177804
110-114	0.05412606880095446
115-119	0.054884207862617676
120-124	0.03685708743295123
125-129	0.02158725080096892
130-134	0.02038527656627305
135-139	0.024030443741845327
140-144	0.03794778384942319
145-149	0.031001656429459953
150-154	0.020572913951702305
155-159	0.01501926596341457
160-164	0.01431720749375656
165-169	0.01826670984787449
170-174	0.008351716709769204
175-179	0.0018800860243805377
180-184	2.2824171710808617E-4
185-189	0.0
190-194	6.730045750851014E-4
195-199	2.8171315403230124E-4
200	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
30-34	93046.0
35-39	63566.0
40-44	48263.0
45-49	38761.0
50-54	33315.0
55-59	35455.0
60-64	34185.0
65-69	35449.0
70-74	35688.0
75-79	35600.0
80-84	35753.0
85-89	36434.0
90-94	36781.0
95-99	36695.0
100-104	62610.0
105-109	31969.0
110-114	30823.0
115-119	29646.0
120-124	28682.0
125-129	27547.0
130-134	27593.0
135-139	26310.0
140-144	25037.0
145-149	23145.0
150-154	21742.0
155-159	21017.0
160-164	19622.0
165-169	18607.0
170-174	17570.0
175-179	16314.0
180-184	15893.0
185-189	15580.0
190-194	4658.0
195-199	10006.0
200-201	135528.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	99.08624911933617
#Duplication Level	Percentage of total
1	98.23940817929194
2	1.6289516424237416
3	0.0793222268775301
4	0.015850849660220663
5	0.0058949122366511834
6	0.003680803899123608
7	0.0
8	0.0
9	0.0
>10	0.0031953618470395764
>50	0.0
>100	0.023696023763757817
>500	0.0
>1k	0.0
>5k	0.0
>10k+	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	0.008520212757157392	0.0	0.0	0.0	0.035321658711710745	0.001158087170875762
2	0.009678299928033155	0.0	0.0	0.0	0.03879592022433803	0.0014062487074919968
3	0.012325356318606324	0.0	0.0	0.0	0.04251834327358155	0.0020680128051352893
4	0.012904399904044206	0.0	0.0	8.272051220541157E-5	0.04872238168898742	0.002564335878367759
5	0.013566164001687498	8.272051220541157E-5	0.0	8.272051220541157E-5	0.05418193549454458	0.0032260999760110516
6	0.013566164001687498	8.272051220541157E-5	0.0	8.272051220541157E-5	0.05922788673907469	0.0035569820248326977
7	0.013648884513892911	8.272051220541157E-5	0.0	8.272051220541157E-5	0.06485288156904268	0.00421874612247599
8	0.013648884513892911	8.272051220541157E-5	0.0	8.272051220541157E-5	0.07039515588680526	0.004797789707913871
9	0.013731605026098322	8.272051220541157E-5	0.0	8.272051220541157E-5	0.0750275045703083	0.005624994829967987
10-14	0.01457535425059352	8.272051220541157E-5	6.617640976432925E-5	8.272051220541157E-5	0.1060807848522198	0.00807352199124817
15-19	0.017404395768018596	1.1580871708757621E-4	1.323528195286585E-4	8.272051220541157E-5	0.22804390804787866	0.011812489142932772
20-24	0.048176426308431705	1.6544102441082314E-4	1.6544102441082314E-4	8.272051220541157E-5	0.4386999644301797	0.014757339377445427
25-29	0.2839133419914136	1.6544102441082314E-4	1.6544102441082314E-4	1.1580871708757621E-4	0.6235803092092747	0.016461381928876903
30-34	0.6502824905491814	1.6544102441082314E-4	1.6544102441082314E-4	1.9852922929298778E-4	0.7518632795374269	0.018347409607160286
35-39	0.7724606870765742	1.6544102441082314E-4	1.8198512685190546E-4	2.4816153661623474E-4	0.8096187411592453	0.019621305495123625
40-44	0.8652565576686051	1.6544102441082314E-4	2.4816153661623474E-4	2.4816153661623474E-4	0.8333098958548752	0.020200349080561508
45-49	0.9579531636459894	2.3161743417515242E-4	2.4816153661623474E-4	2.4816153661623474E-4	0.8458503255052156	0.020613951641588562
50-54	1.046480655808221	2.4816153661623474E-4	2.4816153661623474E-4	2.4816153661623474E-4	0.8527823044280289	0.020779392665999385
55-59	1.1268684495694397	2.4816153661623474E-4	2.977938439394817E-4	3.308820488216463E-4	0.8558429633796292	0.020961377792851288
60-64	1.1978261049392418	2.4816153661623474E-4	3.308820488216463E-4	3.308820488216463E-4	0.8571830356773569	0.02109373061237995
65-69	1.2608095029324422	2.977938439394817E-4	3.308820488216463E-4	3.308820488216463E-4	0.8583907551555559	0.021259171636790777
70-74	1.3149418061196636	3.308820488216463E-4	3.308820488216463E-4	3.308820488216463E-4	0.8588374459214652	0.021374980353878352
75-79	1.3590980155349122	3.9705845858597555E-4	3.308820488216463E-4	3.308820488216463E-4	0.8593006807898155	0.02149078907096593
80-84	1.3985226116520113	4.136025610270579E-4	4.136025610270579E-4	3.308820488216463E-4	0.8597142833508424	0.021755494710023245
85-89	1.4361769888079146	4.136025610270579E-4	4.136025610270579E-4	3.308820488216463E-4	0.8603264151411626	0.02178858291490541
90-94	1.4626144645087642	4.136025610270579E-4	4.136025610270579E-4	3.9705845858597555E-4	0.8614514141071561	0.021838215222228657
95-99	1.4806475361695441	4.136025610270579E-4	4.136025610270579E-4	4.136025610270579E-4	0.8616664874388903	0.021838215222228657
100-104	1.4982835493717377	4.136025610270579E-4	4.136025610270579E-4	4.136025610270579E-4	0.8620800899999171	0.021887847529551904
105-109	1.5177725020473327	4.136025610270579E-4	4.136025610270579E-4	4.136025610270579E-4	0.8625433248682676	0.021920935734434068
110-114	1.5365831465228432	4.963230732324695E-4	4.136025610270579E-4	4.136025610270579E-4	0.863006559736618	0.0219871121441984
115-119	1.556436069452142	4.963230732324695E-4	4.136025610270579E-4	4.797789707913872E-4	0.8635359710147326	0.02200365624663948
120-124	1.5769673005815252	4.963230732324695E-4	4.136025610270579E-4	4.963230732324695E-4	0.8648098669026959	0.022036744451521643
125-129	1.5938753732763113	5.79043585437881E-4	4.136025610270579E-4	4.963230732324695E-4	0.8667289827858614	0.02208637675884489
130-134	1.6114286659662995	5.79043585437881E-4	4.136025610270579E-4	4.963230732324695E-4	0.8682014079031177	0.02208637675884489
135-139	1.6300242371100762	5.79043585437881E-4	4.136025610270579E-4	4.963230732324695E-4	0.8690782453324951	0.02208637675884489
140-144	1.6471970154439195	5.79043585437881E-4	4.136025610270579E-4	4.963230732324695E-4	0.8696407448154918	0.02208637675884489
145-149	1.6638900148069715	5.79043585437881E-4	4.136025610270579E-4	4.963230732324695E-4	0.8700212591716369	0.022152553168609222
150-154	1.6799212500723804	5.79043585437881E-4	4.136025610270579E-4	4.963230732324695E-4	0.8706168468595157	0.022169097271050302
155-159	1.695886308928025	5.79043585437881E-4	4.963230732324695E-4	4.963230732324695E-4	0.8710635376254251	0.022169097271050302
160-164	1.7105774718957059	5.79043585437881E-4	4.963230732324695E-4	4.963230732324695E-4	0.8712455227522768	0.022169097271050302
165-169	1.7244248856388917	5.79043585437881E-4	4.963230732324695E-4	4.963230732324695E-4	0.8713944196742466	0.022169097271050302
170-174	1.737478182464906	5.79043585437881E-4	5.79043585437881E-4	4.963230732324695E-4	0.8716260371084218	0.022169097271050302
175-179	1.7477189818759356	5.79043585437881E-4	5.79043585437881E-4	4.963230732324695E-4	0.8717418458255095	0.022235273680814634
180-184	1.7503329500616267	5.79043585437881E-4	5.79043585437881E-4	4.963230732324695E-4	0.8718907427474791	0.022251817783255714
185-189	1.7505314792909197	5.79043585437881E-4	5.79043585437881E-4	4.963230732324695E-4	0.8720892719767722	0.022251817783255714
>>END_MODULE
