##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	SRR6235896
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	8877888
Total Bases	1 Gbp
Sequences flagged as poor quality	0
Sequence length	30-200
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	34.535613988372006	34.0	31.0	41.0	30.0	41.0
2	34.802931057476734	34.0	31.0	41.0	30.0	41.0
3	34.925797103995905	34.0	31.0	41.0	30.0	41.0
4	37.301084897669355	37.0	35.0	41.0	35.0	41.0
5	37.210826155950606	37.0	35.0	41.0	35.0	41.0
6	37.16771252351911	37.0	35.0	41.0	33.0	41.0
7	37.149304654440336	37.0	35.0	41.0	33.0	41.0
8	37.18585546472314	37.0	35.0	41.0	33.0	41.0
9	38.150032868177654	39.0	38.0	41.0	34.0	41.0
10-14	38.29857885118623	39.4	38.2	41.0	34.0	41.0
15-19	38.8281087123424	41.0	39.0	41.0	34.0	41.0
20-24	38.562796939992936	41.0	39.0	41.0	33.2	41.0
25-29	38.706648856124346	41.0	39.0	41.0	34.0	41.0
30-34	38.66972210235984	41.0	39.0	41.0	33.6	41.0
35-39	38.63778887081302	41.0	39.0	41.0	33.0	41.0
40-44	38.66818785691355	41.0	39.0	41.0	33.2	41.0
45-49	38.562593770030624	41.0	39.0	41.0	33.2	41.0
50-54	38.42824365078886	41.0	38.0	41.0	33.0	41.0
55-59	38.42201625609538	41.0	37.8	41.0	33.0	41.0
60-64	38.23255697516415	41.0	36.8	41.0	32.0	41.0
65-69	38.07021854724178	41.0	35.6	41.0	32.0	41.0
70-74	37.93947810599741	41.0	35.0	41.0	31.0	41.0
75-79	37.92452997343848	41.0	35.0	41.0	31.4	41.0
80-84	38.108042717332395	41.0	35.0	41.0	32.8	41.0
85-89	38.167276174645934	41.0	35.0	41.0	33.0	41.0
90-94	38.016938460079764	41.0	35.0	41.0	32.0	41.0
95-99	37.73163702314891	41.0	35.0	41.0	31.0	41.0
100-104	35.33360072560424	36.6	33.0	39.4	29.4	41.0
105-109	35.43138317015074	37.2	34.0	39.0	30.0	41.0
110-114	35.68278204616221	38.0	34.0	39.2	29.0	41.0
115-119	35.96020065978365	38.0	34.0	40.0	29.0	41.0
120-124	35.95633950690618	38.0	34.0	40.0	28.6	41.0
125-129	35.73724425546426	38.0	34.0	40.0	28.0	41.0
130-134	35.65740922379626	38.0	34.0	40.0	28.0	41.0
135-139	35.73706049105169	38.0	34.0	40.0	28.8	41.0
140-144	35.86673884148988	38.0	34.0	40.0	30.0	41.0
145-149	35.79849477534813	38.0	34.0	40.0	29.4	41.0
150-154	35.36478346815045	37.2	33.6	39.6	28.4	41.0
155-159	35.289601431544966	37.0	34.0	39.4	28.0	41.0
160-164	34.995254471686415	36.2	33.2	39.0	28.6	41.0
165-169	34.63667478485342	35.0	33.0	38.2	29.0	40.2
170-174	34.04492961741648	35.0	33.0	37.0	29.0	39.2
175-179	33.568240187831805	35.0	33.0	35.6	28.6	38.0
180-184	33.13177052375727	35.0	33.0	35.0	28.0	36.8
185-189	32.921723014834065	35.0	32.6	35.0	28.2	36.0
190-194	32.826076014478815	34.8	32.6	35.0	28.2	35.0
195-199	33.01662307756535	35.0	33.0	35.0	29.0	35.0
200	33.27423187387648	35.0	33.0	35.0	29.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	8.0
3	0.0
4	2.0
5	0.0
6	1.0
7	5.0
8	15.0
9	21.0
10	49.0
11	89.0
12	130.0
13	216.0
14	316.0
15	289.0
16	390.0
17	1254.0
18	7474.0
19	18535.0
20	24565.0
21	26018.0
22	27113.0
23	31297.0
24	39545.0
25	53058.0
26	69969.0
27	88822.0
28	107718.0
29	126015.0
30	144915.0
31	163273.0
32	185307.0
33	216575.0
34	262053.0
35	338835.0
36	487189.0
37	662614.0
38	720435.0
39	1333131.0
40	1430606.0
41	2310041.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	19.879507351186984	37.80545278459309	19.615221461505588	22.69981840271434
2	21.951394254549953	34.109240309508166	18.61747552516953	25.32188991077235
3	24.435614007913596	29.170874579326078	19.38966726276807	27.003844149992258
4	27.004333578531043	26.35406023753012	19.304340675371375	27.337265508567466
5	29.374107724122407	24.84563549869273	16.34126138251969	29.438995394665174
6	31.3481116136935	26.582013688454843	17.574371467238432	24.49550323061322
7	28.484510003391744	25.27528491043419	22.551008235595873	23.68919685057819
8	30.302494390325226	28.198179221223945	25.2339617370617	16.265364651389127
9	30.216912445952122	30.101779264425016	24.232307467886674	15.449000821736188
10-14	30.972488792628162	29.886453323028782	21.483947860600892	17.657110023742163
15-19	28.76128323287457	29.857229172273946	21.003576678214888	20.377910916636598
20-24	25.923173093367545	29.316261189131097	22.13816470875099	22.622401008750366
25-29	26.81263405980233	27.744817991360517	22.9066237741382	22.535924174698955
30-34	25.520934757623188	28.00902666425288	22.756586294210475	23.71345228391346
35-39	26.387891451645316	28.79768493755194	22.364917487360596	22.449506123442152
40-44	26.324898882327407	28.335722680010868	22.709380552405847	22.62999788525588
45-49	27.1480003249501	28.17177620357946	22.6542862392834	22.02593723218704
50-54	27.063156600577166	27.93285751502094	23.140948517441622	21.863037366960267
55-59	27.763986863926952	26.856882848989684	23.004832391182305	22.374297895901062
60-64	26.085074178752237	27.387729810592397	24.816154558233457	21.7110414524219
65-69	26.071177640383663	27.79337441289854	24.527359934028294	21.6080880126895
70-74	26.200234985118936	30.36891553083141	22.411861369437002	21.018988114612654
75-79	27.27461848246805	29.53669021756936	22.992732879382256	20.195958420580336
80-84	28.012773488956437	28.03807671065735	22.62786827053094	21.32128152985527
85-89	27.66050229364932	28.597726963573635	22.584083618493423	21.157687124283626
90-94	28.761642422239603	27.719877830255484	22.475812018968604	21.04266772853631
95-99	28.521559284419894	26.960896481273274	23.030206912701047	21.487337321605782
100-104	29.142214854558386	24.85642088815475	22.300281267202266	23.701082990084597
105-109	27.545589387522888	27.732425438000597	23.99830078312231	20.723684391354208
110-114	26.625443658106256	27.259245153035895	25.28647540788953	20.828835780968312
115-119	26.40640879492029	25.794544315580513	25.171370495084126	22.62767639441507
120-124	27.00622705326702	24.482493502112945	25.294459966235056	23.216819478384977
125-129	26.91928588330948	24.5028325862569	25.339142310708084	23.23873921972553
130-134	24.842013972843073	26.019200844931568	24.92477212469221	24.214013057533144
135-139	22.394745410197718	28.646072569630597	26.212863742195264	22.74631827797642
140-144	22.338694808486487	31.85538400405058	24.12397764787579	21.681943539587138
145-149	22.976276606667724	30.07439123318671	24.110062510327158	22.839269649818412
150-154	24.25105497813009	26.32350068754679	24.973541228660682	24.45190310566244
155-159	24.022140077635726	24.6187260797422	26.11025060832833	25.248883234293746
160-164	23.13166327725396	24.999810382505892	26.55224436006466	25.316281980175493
165-169	23.53492942938238	25.19188168419777	26.379117301994448	24.894071584425408
170-174	23.392147619848863	24.847312768955373	26.969927519323257	24.790612091872507
175-179	23.511795944872464	24.876593672871046	25.804359877724924	25.807250504531567
180-184	21.565998073334374	24.827060579519205	27.21172518551917	26.39521616162725
185-189	21.538205235842575	23.713939343740766	27.291947073074773	27.45590834734189
190-194	20.895781490124257	24.67337913748577	28.426908164380375	26.003931208009597
195-199	21.304425187065313	24.997656596862253	27.328318357069527	26.369599859002907
200	20.01880281835651	26.320993922291525	29.957287789384818	23.70291546996715
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	255.0
1	174.5
2	70.0
3	45.5
4	47.5
5	50.5
6	57.5
7	74.5
8	107.5
9	143.0
10	185.66666666666669
11	275.16666666666663
12	413.0
13	576.5000000000001
14	810.3333333333337
15	1159.9999999999986
16	1589.9999999999964
17	2225.333333333345
18	3200.0000000000205
19	4304.166666666602
20	5678.499999999888
21	7670.666666666491
22	10398.000000000513
23	13858.833333334132
24	18144.166666664485
25	23633.66666666353
26	29932.83333332874
27	37516.16666667892
28	46836.33333334909
29	56529.83333335323
30	66559.66666667223
31	77101.16666660868
32	89153.66666659828
33	102022.16666658559
34	114398.16666657657
35	127271.6666665677
36	138730.66666683106
37	148135.83333355872
38	156325.83333357284
39	164139.50000023912
40	172230.5000002392
41	182414.16666689774
42	198766.00000022838
43	214065.5000002244
44	230740.50000021857
45	257085.66666688753
46	297472.3333328494
47	354618.49999945576
48	430577.16666603036
49	526047.4999993051
50	611523.000001908
51	673056.1666689303
52	695901.1666691917
53	674145.3333360043
54	629581.8333358929
55	560906.5000006135
56	477960.83333243616
57	404828.9999992794
58	340296.33333280246
59	285461.3333329696
60	239632.5000001302
61	191985.3333334233
62	152408.66666672102
63	122260.49999998219
64	97860.66666665464
65	78358.8333333249
66	62563.8333333365
67	52211.666666669036
68	44806.0000000017
69	38657.6666666677
70	34493.5000000006
71	30807.49999999981
72	27052.66666666655
73	23575.499999999945
74	20992.66666666664
75	18701.333333333325
76	16122.66666666667
77	13947.666666666668
78	12047.5
79	10382.5
80	9305.0
81	8583.5
82	6681.0
83	4629.0
84	4159.0
85	3720.0
86	3165.0
87	2802.5
88	2427.0
89	2011.5
90	1692.0
91	1569.0
92	1208.5
93	782.0
94	661.5
95	481.5
96	275.5
97	218.5
98	140.0
99	45.0
100	22.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	2.252788050491288E-5
2	0.007963605758486703
3	0.008594386412624264
4	0.007862230296214596
5	0.011365315714728548
6	0.007051226598037731
7	0.005057509173352942
8	0.00414513001290397
9	0.003863531506592559
10-14	0.005821204322469489
15-19	0.01999349394811018
20-24	0.21855423271841232
25-29	0.0015048624177281804
30-34	0.001796702076074257
35-39	0.0011968704709673335
40-44	0.0019532323596632385
45-49	7.000626914798239E-4
50-54	0.001704460987913657
55-59	0.006147558195553054
60-64	0.0031612503997703015
65-69	0.0013514614050924854
70-74	0.012450518303299449
75-79	0.007463314381437603
80-84	0.003707193054090066
85-89	0.0022460393733291237
90-94	0.0017925570840487597
95-99	0.004209126105090975
100-104	0.018842199085233106
105-109	0.022410735642860894
110-114	0.01753856505683775
115-119	0.0210028104361451
120-124	0.015635590694212363
125-129	0.011519274709819744
130-134	0.015336363930876947
135-139	0.01975649070058164
140-144	0.01945468321762727
145-149	0.01170274218960941
150-154	0.013010176577713615
155-159	0.026086052379624663
160-164	0.03269832243389252
165-169	0.005525772724057732
170-174	0.004195645519328868
175-179	0.0035936231001842747
180-184	0.004047308925430905
185-189	0.0026541820968452694
190-194	0.0020748380243115687
195-199	2.8064700360210433E-5
200	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
30-34	450255.0
35-39	165557.0
40-44	124906.0
45-49	136332.0
50-54	170155.0
55-59	171710.0
60-64	188413.0
65-69	230948.0
70-74	284993.0
75-79	313682.0
80-84	321324.0
85-89	343743.0
90-94	330384.0
95-99	359215.0
100-104	423588.0
105-109	319097.0
110-114	303318.0
115-119	303363.0
120-124	296250.0
125-129	256830.0
130-134	279972.0
135-139	297309.0
140-144	310884.0
145-149	348443.0
150-154	228603.0
155-159	168327.0
160-164	154849.0
165-169	141564.0
170-174	126550.0
175-179	118057.0
180-184	103880.0
185-189	113722.0
190-194	260469.0
195-199	53638.0
200-201	677558.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	39.14893383010608
#Duplication Level	Percentage of total
1	26.27551986721363
2	11.774617272479636
3	8.680468127417367
4	6.152664013202515
5	4.180559462457638
6	2.777673335757392
7	1.861366133392783
8	1.3580611337100243
9	0.9753738706113161
>10	11.299355475261674
>50	4.6187136419606825
>100	10.128969414317734
>500	3.417800536925745
>1k	5.389449562558654
>5k	1.1094081527332857
>10k+	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AGCAGAAAGGTTTGGGAAAGCCTGCCGGAGAGGGTGAAAGCCCCGTAAGC	9870	0.11117509029174505	No Hit
AATGCACCCGGTGAAATTGAAATACCAGTGAAGATGCTGGTTACCTGCGC	9631	0.10848300857140797	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	0.34724475010272715	0.0	0.0	0.0	3.0412638681632387E-4	3.0412638681632387E-4
2	0.4555475356300958	0.0	0.0	0.0	0.0016220073963537274	3.717100283310625E-4
3	0.6410984234088107	1.126394025245644E-5	0.0	0.0	0.0020613010661995287	3.8297396858351894E-4
4	0.8247682331653654	2.252788050491288E-5	0.0	0.0	0.002613234138569894	5.068773113605398E-4
5	1.3535877001376904	2.252788050491288E-5	0.0	0.0	0.003300334493969737	5.294051918654527E-4
6	2.4720293835651	2.252788050491288E-5	0.0	0.0	0.004347880937448186	5.406691321179091E-4
7	4.6775088849960715	2.252788050491288E-5	0.0	0.0	0.005406691321179091	5.744609528752784E-4
8	6.149379221724806	2.252788050491288E-5	0.0	1.126394025245644E-5	0.007208921761572121	6.6457247489493E-4
9	6.5020982467902275	2.252788050491288E-5	1.126394025245644E-5	1.126394025245644E-5	0.00898862432146024	6.871003553998429E-4
10-14	6.7153268885572786	2.252788050491288E-5	1.126394025245644E-5	2.252788050491288E-5	0.014280423452064275	8.312787906312853E-4
15-19	6.845233911488859	3.379182075736932E-5	1.126394025245644E-5	2.252788050491288E-5	0.025359635084380428	0.0011196356610941702
20-24	7.034231564984824	4.9561337110808326E-5	7.884758176719509E-5	2.252788050491288E-5	0.03510744897885623	0.0013494200422442817
25-29	7.25623256342049	9.011152201965152E-5	1.0137546227210795E-4	2.252788050491288E-5	0.04088134475226541	0.0014778289611222849
30-34	7.393215593618663	9.68698861711254E-5	1.0137546227210795E-4	2.252788050491288E-5	0.0477343260018599	0.001617501820252745
35-39	7.469046692186249	1.1038661447407312E-4	1.0813382642358182E-4	3.1539032706878036E-5	0.052064184634904156	0.0017797025598881174
40-44	7.527948088554394	1.1263940252456438E-4	1.1263940252456438E-4	4.055018490884318E-5	0.05310947829033212	0.0018743196580087516
45-49	7.587234711679173	1.1263940252456438E-4	1.1263940252456438E-4	4.505576100982576E-5	0.05372674221616673	0.001932892147321525
50-54	7.675102456800536	1.1263940252456438E-4	1.1263940252456438E-4	4.505576100982576E-5	0.05411647454890173	0.0019621783919779117
55-59	7.838271895297621	1.5769516353439017E-4	1.1263940252456438E-4	4.505576100982576E-5	0.054530987550192124	0.002004981364937246
60-64	7.995935519799303	1.5769516353439017E-4	1.1263940252456438E-4	4.505576100982576E-5	0.05526764924270276	0.0020545427020480546
65-69	8.123634810441402	1.5769516353439017E-4	1.1263940252456438E-4	4.505576100982576E-5	0.06411660070503254	0.002068059430351003
70-74	8.249716599263248	2.1401486479667237E-4	1.1263940252456438E-4	4.505576100982576E-5	0.08551132882054831	0.002101851251108371
75-79	8.37036691609536	2.1401486479667237E-4	1.1263940252456438E-4	4.505576100982576E-5	0.08903243654346618	0.002223501805834901
80-84	8.531418733824982	2.1401486479667237E-4	1.1263940252456438E-4	4.505576100982576E-5	0.08940639935984775	0.0030007136832543957
85-89	8.674887540820519	2.478066855540417E-4	1.1263940252456438E-4	4.505576100982576E-5	0.08999437704102597	0.004165405105358391
90-94	8.710711376399432	2.478066855540417E-4	1.1263940252456438E-4	4.505576100982576E-5	0.09088197553291955	0.004606951563254684
95-99	8.718497011901928	2.81598506311411E-4	1.1263940252456438E-4	4.505576100982576E-5	0.09106219857695884	0.004618215503507141
100-104	8.726451606508215	2.81598506311411E-4	1.1263940252456438E-4	4.505576100982576E-5	0.09122890489269521	0.004654260112315001
105-109	8.735437978041622	2.81598506311411E-4	1.1263940252456438E-4	4.505576100982576E-5	0.0915645703122184	0.0046835463569713884
110-114	8.742878936972398	2.81598506311411E-4	1.1263940252456438E-4	4.505576100982576E-5	0.09205342531917501	0.004712832601627774
115-119	8.749143940540813	2.81598506311411E-4	1.1263940252456438E-4	4.505576100982576E-5	0.09262788627205029	0.0047240965418802305
120-124	8.754453761975821	2.8385129436190226E-4	1.1263940252456438E-4	4.505576100982576E-5	0.09375878587339691	0.004730854906031705
125-129	8.758770103880561	2.9286244656386744E-4	1.1263940252456438E-4	4.505576100982576E-5	0.09536952932949819	0.0047578883626376006
130-134	8.762086207890887	3.1088475096779775E-4	1.1263940252456438E-4	4.505576100982576E-5	0.09764484526049438	0.004775910667041531
135-139	8.765262639042078	3.1539032706878034E-4	1.1263940252456438E-4	4.505576100982576E-5	0.10061401991104191	0.004787174607293988
140-144	8.767963731914616	3.1539032706878034E-4	1.1263940252456438E-4	4.505576100982576E-5	0.10489431720697535	0.004829977580253321
145-149	8.770658066423005	3.1539032706878034E-4	1.1263940252456438E-4	4.505576100982576E-5	0.10653209411968252	0.004875033341263147
150-154	8.77430533027675	3.1539032706878034E-4	1.1263940252456438E-4	4.505576100982576E-5	0.10685199002285228	0.0048772861293136386
155-159	8.778328809734926	3.1539032706878034E-4	1.1263940252456438E-4	4.505576100982576E-5	0.10703221306689159	0.0048772861293136386
160-164	8.781933270615713	3.1539032706878034E-4	1.1263940252456438E-4	4.505576100982576E-5	0.10736112012226331	0.0048772861293136386
165-169	8.785652623687076	3.221486912202542E-4	1.1263940252456438E-4	4.505576100982576E-5	0.10775310524304879	0.0048772861293136386
170-174	8.788756965620653	3.2665426732123677E-4	1.1263940252456438E-4	4.505576100982576E-5	0.10789953646633073	0.0048772861293136386
175-179	8.791795976700765	3.2665426732123677E-4	1.1263940252456438E-4	4.505576100982576E-5	0.1080121758688553	0.0048772861293136386
180-184	8.793426995249321	3.2665426732123677E-4	1.1263940252456438E-4	4.505576100982576E-5	0.10809327623867297	0.0048772861293136386
185-189	8.793645515690217	3.2665426732123677E-4	1.2165055472652955E-4	4.505576100982576E-5	0.1082937743751667	0.0048772861293136386
>>END_MODULE
