FastQCFastQC Report
Wed 13 Sep 2023
stdin:SRR6235898

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6235898
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3817029
Total Bases420.3 Mbp
Sequences flagged as poor quality0
Sequence length30-200
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATG216140.566251920014231TruSeq Adapter, Index 12 (100% over 49bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGC194330.5091132396426645TruSeq Adapter, Index 12 (100% over 50bp)
GAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGCCGTCT48540.12716696676918096TruSeq Adapter, Index 12 (100% over 50bp)
CAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTAT38400.10060180313013078TruSeq Adapter, Index 12 (100% over 48bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)