FastQCFastQC Report
Wed 13 Sep 2023
stdin:SRR6235900

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6235900
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2778994
Total Bases236 Mbp
Sequences flagged as poor quality0
Sequence length30-200
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATG90300.3249377292646188TruSeq Adapter, Index 7 (100% over 49bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGC62180.2237500332854263TruSeq Adapter, Index 7 (100% over 50bp)
TTGTTGGCGATGTGGAGACGGAGGAGTGACACTTCCGCGAGCTGACGGAA40140.14444075805849166No Hit
ATCGTGGGCGCAGGAAATTTGCGTGGCTCTGACACTAGTACGAGAGGACC38550.138719263157819No Hit
GCACGAATGGTGAAATGATTTGGGAGCTGTCTTGGCTGGAGACCTGGTGA32910.11842414917052717No Hit
CATAAGGCTAAATACTCTTACTAACCGATAGCGCATAGTACCGTGAGGGA27920.10046801108602608No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)