##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	SRR6235904
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	183309
Total Bases	12.4 Mbp
Sequences flagged as poor quality	0
Sequence length	30-200
%GC	30
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	25.42397809163762	28.0	16.0	33.0	10.0	34.0
2	25.067776268486544	28.0	16.0	34.0	10.0	36.0
3	24.964688040412636	28.0	16.0	34.0	10.0	41.0
4	27.12500204572607	32.0	19.0	37.0	10.0	41.0
5	26.981064759504445	32.0	19.0	37.0	10.0	41.0
6	26.7398982046708	32.0	17.0	37.0	10.0	41.0
7	26.752243479589108	32.0	17.0	37.0	10.0	41.0
8	26.66978162556121	32.0	17.0	37.0	10.0	41.0
9	27.215324943128817	32.0	17.0	39.0	10.0	41.0
10-14	27.191256294017204	31.6	16.8	39.2	10.0	41.0
15-19	27.340117506505408	31.0	16.2	40.0	10.0	41.0
20-24	27.598081927237615	31.0	16.6	40.0	10.0	41.0
25-29	28.202981850318317	31.8	17.0	40.0	10.0	41.0
30-34	29.76434550207846	33.0	22.2	40.4	9.2	41.0
35-39	31.348204654812243	35.2	25.2	41.0	9.2	41.0
40-44	32.94674842374779	38.0	29.4	41.0	14.6	41.0
45-49	34.56714214695626	39.8	31.4	41.0	17.8	41.0
50-54	35.734724426754106	40.4	33.8	41.0	23.0	41.0
55-59	36.85396485312974	41.0	36.2	41.0	26.8	41.0
60-64	37.668524239521034	41.0	37.8	41.0	30.2	41.0
65-69	38.04293748335937	41.0	38.0	41.0	31.2	41.0
70-74	38.247972432903964	41.0	38.0	41.0	32.0	41.0
75-79	38.110674031255314	41.0	37.2	41.0	31.8	41.0
80-84	38.30331307050231	41.0	37.0	41.0	32.8	41.0
85-89	38.255352036267205	41.0	36.6	41.0	33.0	41.0
90-94	38.13209912414523	41.0	36.0	41.0	33.0	41.0
95-99	37.870612037817004	41.0	35.2	41.0	33.0	41.0
100-104	36.26733942073282	37.8	34.2	40.2	31.4	41.0
105-109	36.88294987181534	37.4	36.0	40.0	32.0	41.0
110-114	37.49730471780365	39.2	37.0	40.2	32.4	41.0
115-119	37.90144722210196	40.0	37.0	41.0	33.0	41.0
120-124	37.890401252955705	40.0	37.0	41.0	32.4	41.0
125-129	37.795157989101426	40.0	37.0	41.0	32.0	41.0
130-134	37.81655726950392	40.0	37.0	41.0	32.2	41.0
135-139	37.86484108367755	40.0	37.0	41.0	33.0	41.0
140-144	37.71064932032476	40.0	37.0	41.0	32.0	41.0
145-149	37.582736635750884	39.8	37.0	41.0	31.6	41.0
150-154	37.220787798997534	39.0	36.4	40.6	31.0	41.0
155-159	37.51670654393249	39.8	37.0	41.0	31.2	41.0
160-164	37.71796753129596	40.0	37.0	41.0	32.4	41.0
165-169	37.3739006087721	39.0	36.0	41.0	31.4	41.0
170-174	37.08013148652756	39.0	35.0	41.0	31.0	41.0
175-179	37.069336056282296	39.0	35.0	41.0	31.0	41.0
180-184	36.90210412651113	38.0	35.0	41.0	31.0	41.0
185-189	36.718308505879165	38.0	35.0	41.0	31.0	41.0
190-194	36.47806841035158	37.4	35.0	39.8	31.0	41.0
195-199	36.03115519839693	36.8	35.0	39.0	31.4	40.0
200	35.844530493707644	36.0	35.0	39.0	32.0	39.0
>>END_MODULE
>>Per sequence quality scores	fail
#Quality	Count
8	1.0
9	3.0
10	7.0
11	9.0
12	29.0
13	34.0
14	54.0
15	53.0
16	85.0
17	773.0
18	7716.0
19	20481.0
20	23609.0
21	19236.0
22	14013.0
23	9633.0
24	6726.0
25	4947.0
26	3700.0
27	2835.0
28	2314.0
29	1949.0
30	1628.0
31	1503.0
32	1428.0
33	1564.0
34	1769.0
35	2183.0
36	3157.0
37	4150.0
38	6378.0
39	12376.0
40	13120.0
41	15846.0
>>END_MODULE
>>Per base sequence content	pass
#Base	G	A	T	C
1	20.27014494651108	35.167394945147265	27.07013840018766	17.49232170815399
2	20.647252015843055	32.832327684972014	29.667535925105565	16.85288437407937
3	18.432827676525154	32.78402269998909	29.338098875914003	19.445050747571756
4	18.02209794025372	32.51834674669213	31.143363797571954	18.3161915154822
5	17.64593552047926	32.45036364529171	30.98323357867342	18.920467255555618
6	17.477873691792258	33.03995285541235	31.335872447698975	18.14630100509642
7	17.06280351394118	32.91919026572816	32.011240246630656	18.006765973700006
8	17.539025627874747	33.21802517500832	31.203588011589016	18.039361185527916
9	17.227307939902456	33.294235742108654	30.956017937611158	18.522438380377736
10-14	17.231036792800737	33.2578681953176	31.8670911626141	17.64400384926757
15-19	16.84075001500248	33.52842015678958	32.44158578964883	17.189244038559107
20-24	16.21339117569399	34.09898812238548	32.93883126717104	16.748789434749497
25-29	15.499676487692893	34.75155018586955	33.518493665596296	16.230279660841262
30-34	15.32280362835103	35.184212994485854	33.761034082479576	15.731949294683542
35-39	15.192111353388368	35.208707964940054	33.963962098563485	15.63521858310809
40-44	14.977301308227847	35.16954883196105	34.33467298340946	15.518476876401637
45-49	14.825685816213557	34.99200393652356	34.826669947102964	15.35564030015992
50-54	14.579348261840675	34.946738445678946	35.25284920680373	15.22106408567665
55-59	14.565299209634928	34.945269037234766	35.518038604226035	14.971393148904275
60-64	14.483276450511944	35.34641638225256	35.41399317406143	14.75631399317406
65-69	14.326137192235278	35.655542939232454	35.484315653148656	14.53400421538361
70-74	14.301525450250171	35.85056159620794	35.62538880471413	14.222524148827768
75-79	14.378599515430752	35.753664058466065	35.80092941970847	14.066807006394724
80-84	14.136687983012767	35.836975218718884	36.09417051430829	13.932166283960068
85-89	13.978595408919716	35.955793384345334	36.239540551134134	13.82607065560081
90-94	13.759866356717962	36.48541149226159	36.17119068032562	13.58353147069482
95-99	13.565436666252525	36.611493808583774	36.47850835415545	13.344561171008252
100-104	12.723751493564226	37.43112616158241	37.07946611095696	12.765656233896404
105-109	12.621431219873935	37.269805957236436	37.23087381040662	12.877889012483006
110-114	12.32340151393139	37.510710259301014	37.56869061040425	12.597197616363342
115-119	12.33461201965139	37.61154855643045	37.542230298135806	12.511609125782355
120-124	12.26899891293606	37.66676549066113	37.450058588511006	12.614177007891803
125-129	12.180082463504327	37.74896920619591	37.6293599791984	12.441588351101371
130-134	12.073620594929753	37.92951887606939	37.665803312141904	12.331057216858959
135-139	12.229234747756962	38.0605516335304	37.62233808133975	12.08787553737288
140-144	11.969517687158929	38.23004786041008	37.704642989597865	12.095791462833125
145-149	11.982175899701673	38.08579736414788	37.919640496960085	12.012386239190363
150-154	12.034877826219203	38.4862617864747	37.62749670485653	11.85136368244956
155-159	12.039744746540272	38.43804015784839	37.71619902812354	11.806016067487796
160-164	11.90074906367041	38.41760299625468	37.833567415730336	11.848080524344569
165-169	12.256045014447205	38.42069245196938	37.34729051553708	11.975972018046333
170-174	12.199681125955248	38.626367584804	37.159821870361206	12.014129418879541
175-179	12.190088823758112	38.168795047402575	37.54672967072405	12.094386458115261
180-184	12.3709664309518	38.18905957896448	37.05275136145655	12.387222628627164
185-189	12.00176600441501	38.34172185430463	37.506401766004416	12.150110375275938
190-194	11.91906417957635	38.31991222034182	37.854977590150824	11.906046009931003
195-199	11.444410692588091	38.53280680437424	38.1455042527339	11.877278250303766
200	11.655372700871249	38.16069699903195	38.35430784123911	11.829622458857695
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	2032.0
1	1716.0
2	1197.0
3	1016.5
4	1179.0
5	1544.0
6	1905.5
7	2228.1666666666665
8	2664.666666666667
9	3097.166666666667
10	3592.666666666666
11	4277.833333333336
12	5078.999999999997
13	5799.666666666657
14	6404.999999999989
15	6969.999999999994
16	7251.333333333329
17	7367.499999999996
18	7387.5
19	7257.166666666667
20	7137.166666666666
21	7070.833333333334
22	7109.166666666667
23	7268.333333333334
24	7404.0
25	7534.166666666667
26	7718.0
27	7765.0
28	7752.5
29	7819.0
30	7974.666666666666
31	8170.333333333333
32	8295.5
33	8304.5
34	8346.166666666666
35	8464.666666666666
36	8555.0
37	8679.0
38	8771.666666666666
39	8685.0
40	8543.666666666666
41	8403.0
42	8187.333333333333
43	7920.833333333333
44	7693.0
45	7391.333333333333
46	7114.666666666666
47	6893.0
48	6645.166666666666
49	6387.166666666666
50	6035.5
51	5595.5
52	5218.166666666666
53	4884.5
54	4514.166666666666
55	4185.666666666667
56	3892.5
57	3620.8333333333335
58	3390.666666666667
59	3176.666666666667
60	2896.1666666666665
61	2665.333333333333
62	2497.833333333333
63	2270.1666666666665
64	2054.833333333333
65	1846.1666666666665
66	1652.3333333333333
67	1476.8333333333333
68	1289.0
69	1113.3333333333333
70	982.3333333333333
71	879.5
72	752.5
73	615.8333333333333
74	529.0
75	484.5
76	446.0
77	388.0
78	329.0
79	293.0
80	260.0
81	236.5
82	220.5
83	204.0
84	196.0
85	176.5
86	151.0
87	135.0
88	119.5
89	107.5
90	98.5
91	90.5
92	81.0
93	74.5
94	71.0
95	63.5
96	55.0
97	48.5
98	40.0
99	36.5
100	39.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.006000796469349568
3	0.02673082063619353
4	0.018547916359807754
5	0.014183700745735344
6	0.02345765892563922
7	0.021275551118603016
8	0.017456862456289652
9	0.003818688662313362
10-14	7.637377324626723E-4
15-19	0.00327316171055431
20-24	0.0033822671009061203
25-29	0.004582426394776034
30-34	0.003045052155653322
35-39	0.0035039602714613586
40-44	0.004036954279474308
45-49	0.0054124269322364145
50-54	0.013743028276280678
55-59	0.03003686630116543
60-64	0.007507968684945157
65-69	0.00906841941077038
70-74	0.009158732278807076
75-79	0.015090862879654339
80-84	0.014812254672031993
85-89	0.0
90-94	4.4194011711413104E-4
95-99	0.0023007334738314573
100-104	0.013588879766724231
105-109	0.016064654053853662
110-114	0.009018352347026198
115-119	0.01009387301907742
120-124	0.001411751418810176
125-129	0.01114247511513891
130-134	0.004708984742889433
135-139	0.011639991685720224
140-144	0.007063768167128755
145-149	0.0018881105677548476
150-154	0.026354200454103145
155-159	0.02282410225197809
160-164	0.014042971492767871
165-169	0.029139374897061993
170-174	0.026107866712469945
175-179	0.0029906094862132905
180-184	0.0
185-189	0.0035318840835643775
190-194	0.0
195-199	0.0
200	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
30-34	45050.0
35-39	30088.0
40-44	20975.0
45-49	13871.0
50-54	7783.0
55-59	5313.0
60-64	3854.0
65-69	3023.0
70-74	2219.0
75-79	1844.0
80-84	1690.0
85-89	1659.0
90-94	1698.0
95-99	1859.0
100-104	9473.0
105-109	1331.0
110-114	1337.0
115-119	1354.0
120-124	1400.0
125-129	1410.0
130-134	1470.0
135-139	1376.0
140-144	1464.0
145-149	1459.0
150-154	1372.0
155-159	1333.0
160-164	1295.0
165-169	1271.0
170-174	1203.0
175-179	1121.0
180-184	1016.0
185-189	888.0
190-194	165.0
195-199	315.0
200-201	10330.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	86.68132312715659
#Duplication Level	Percentage of total
1	82.74687218933767
2	3.367611015651248
3	1.8377655956439747
4	1.666128117500587
5	1.6533819992792944
6	1.4255463848256795
7	1.2352046120958649
8	0.9747497265265793
9	0.917356280454847
>10	3.116641393996176
>50	0.44733173790247144
>100	0.6114109467856172
>500	0.0
>1k	0.0
>5k	0.0
>10k+	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	0.004909742565831465	0.0	0.0	0.0	0.919212913714002	0.02018449721508491
2	0.005455269517590517	0.0	0.0	0.0	0.9650371776617623	0.02236660502212112
3	0.00654632342110862	0.0	0.0	0.0	1.0359556813904391	0.02945845539498879
4	0.008182904276385775	0.0	0.0	0.0	1.1063286581673568	0.036550305767856464
5	0.008728431228144826	0.0	0.0	0.0	1.1663366228608525	0.042551102237206026
6	0.009273958179903879	0.0	0.0	0.0	1.2323453840236978	0.048551898706555595
7	0.00981948513166293	0.0	0.0	0.0	1.2825338635855303	0.054552695175905164
8	0.011456065986940085	0.0	0.0	5.455269517590516E-4	1.3392686665684719	0.061644545548772835
9	0.012547119890458189	0.0	0.0	5.455269517590516E-4	1.4085505894418713	0.0654632342110862
10-14	0.013638173793976291	0.0	0.0	5.455269517590516E-4	1.77852696812486	0.08673878532968922
15-19	0.017784178627345087	0.0	0.0	5.455269517590516E-4	2.905258334288005	0.11543350299221533
20-24	0.03644120037750465	0.0	0.0	5.455269517590516E-4	4.360396925410099	0.13474515708448578
25-29	0.12459835578176741	0.0	0.0	5.455269517590516E-4	5.3920974965768185	0.14554659072931497
30-34	0.23272179762041145	0.0	0.0	5.455269517590516E-4	6.028509238498929	0.15176559797936817
35-39	0.2615256206732893	0.0	0.0	5.455269517590516E-4	6.3535341963569705	0.15678444593555146
40-44	0.28040085320415253	0.0	0.0	5.455269517590516E-4	6.5210109705470005	0.16103955615927204
45-49	0.29916698034466394	0.0	0.0	5.455269517590516E-4	6.601094327065228	0.16245792623384558
50-54	0.31378710265180654	0.0	0.0	5.455269517590516E-4	6.644954693986657	0.1630034531856046
55-59	0.3276434872264864	0.0	0.0	5.455269517590516E-4	6.664702769640334	0.1636580855277155
60-64	0.34368197960820257	0.0	0.0	5.455269517590516E-4	6.676158835627274	0.16409450708912274
65-69	0.3579747857442897	0.0	0.0	5.455269517590516E-4	6.687396690833512	0.16420361247947454
70-74	0.3694308517312298	0.0	0.0	5.455269517590516E-4	6.695470489719544	0.16431271786982635
75-79	0.3794685476435964	0.0	0.0	5.455269517590516E-4	6.699725599943266	0.1647491394312336
80-84	0.38961534894631467	0.0	0.0	5.455269517590516E-4	6.7038716047766345	0.1647491394312336
85-89	0.39910751790692217	0.0	0.0	5.455269517590516E-4	6.70539908024156	0.1657310879443999
90-94	0.40510831437627176	0.0	0.0	5.455269517590516E-4	6.707144766487188	0.16638572028651075
95-99	0.4110000054552695	0.0	0.0	5.455269517590516E-4	6.708781347342464	0.16638572028651075
100-104	0.41700080192461914	0.0	0.0	5.455269517590516E-4	6.714345722250409	0.16638572028651075
105-109	0.4232198091746723	0.0	0.0	5.455269517590516E-4	6.715545881544277	0.1667130364575662
110-114	0.42823865713085557	0.0	0.0	5.455269517590516E-4	6.716636935447797	0.1669312472382698
115-119	0.43031165954753997	0.0	0.0	5.455269517590516E-4	6.71805530552237	0.1669312472382698
120-124	0.4339121374291497	0.0	0.0	5.455269517590516E-4	6.71925546481624	0.1669312472382698
125-129	0.4381672476528703	0.0	0.0	5.455269517590516E-4	6.720237413329405	0.1669312472382698
130-134	0.445259098025738	0.0	0.0	5.455269517590516E-4	6.720782940281164	0.1669312472382698
135-139	0.4492959974687549	0.0	0.0	5.455269517590516E-4	6.720892045671516	0.1669312472382698
140-144	0.456169637060919	0.0	0.0	5.455269517590516E-4	6.721546678013628	0.1669312472382698
145-149	0.46107937962675044	0.0	0.0	5.455269517590516E-4	6.723074153478552	0.1669312472382698
150-154	0.46413433055660114	0.0	0.0	5.455269517590516E-4	6.723074153478552	0.1669312472382698
155-159	0.4690440731224326	0.0	0.0	5.455269517590516E-4	6.723401469649607	0.1669312472382698
160-164	0.47471755342072675	0.0	0.0	5.455269517590516E-4	6.723728785820663	0.1669312472382698
165-169	0.482027614574298	0.0	0.0	5.455269517590516E-4	6.72416520738207	0.1669312472382698
170-174	0.48955588650857296	0.0	0.0	5.455269517590516E-4	6.72416520738207	0.1669312472382698
175-179	0.4939201021226453	0.0	0.0	5.455269517590516E-4	6.72416520738207	0.1669312472382698
180-184	0.49479294524545986	0.0	0.0	5.455269517590516E-4	6.7242743127724225	0.1669312472382698
185-189	0.49479294524545986	0.0	0.0	5.455269517590516E-4	6.72471073433383	0.1669312472382698
>>END_MODULE
