##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	SRR6451679
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	543902
Total Bases	163.1 Mbp
Sequences flagged as poor quality	0
Sequence length	300
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.156925328459906	32.0	32.0	32.0	32.0	32.0
2	31.434852234409874	32.0	32.0	32.0	32.0	32.0
3	34.99223941077621	37.0	32.0	37.0	32.0	37.0
4	35.70305680067365	37.0	37.0	37.0	32.0	37.0
5	36.034807741100416	37.0	37.0	37.0	32.0	37.0
6	39.24791230773191	41.0	41.0	41.0	37.0	41.0
7	39.57149633573695	41.0	41.0	41.0	37.0	41.0
8	39.642507289916196	41.0	41.0	41.0	37.0	41.0
9	39.68116866641417	41.0	41.0	41.0	37.0	41.0
10-14	39.703354280734395	41.0	41.0	41.0	37.0	41.0
15-19	39.67225198657111	41.0	41.0	41.0	37.0	41.0
20-24	39.654970564550226	41.0	41.0	41.0	37.0	41.0
25-29	39.508155145596085	41.0	41.0	41.0	37.0	41.0
30-34	39.38337016594902	41.0	41.0	41.0	37.0	41.0
35-39	39.393198039352676	41.0	41.0	41.0	37.0	41.0
40-44	39.30618567315435	41.0	41.0	41.0	37.0	41.0
45-49	39.27822696000383	41.0	41.0	41.0	37.0	41.0
50-54	39.26213104566632	41.0	41.0	41.0	37.0	41.0
55-59	39.08556651749764	41.0	41.0	41.0	37.0	41.0
60-64	39.12143106662597	41.0	41.0	41.0	37.0	41.0
65-69	39.09617026596703	41.0	41.0	41.0	36.0	41.0
70-74	38.872068129920464	41.0	41.0	41.0	33.0	41.0
75-79	38.638930174921214	41.0	39.4	41.0	34.0	41.0
80-84	39.19489062367853	41.0	41.0	41.0	37.0	41.0
85-89	39.19976723748028	41.0	41.0	41.0	36.0	41.0
90-94	39.10658133266655	41.0	41.0	41.0	36.0	41.0
95-99	39.11744762843306	41.0	41.0	41.0	36.0	41.0
100-104	39.024091104647525	41.0	41.0	41.0	36.0	41.0
105-109	38.90828200668503	41.0	41.0	41.0	32.0	41.0
110-114	38.6152692948362	41.0	41.0	41.0	32.0	41.0
115-119	38.721556456861705	41.0	41.0	41.0	32.0	41.0
120-124	38.615398729918255	41.0	41.0	41.0	32.0	41.0
125-129	38.47983644112359	41.0	39.4	41.0	32.0	41.0
130-134	38.23517471897511	41.0	37.0	41.0	32.0	41.0
135-139	38.02848674945119	41.0	37.0	41.0	32.0	41.0
140-144	37.68369375365415	41.0	37.0	41.0	29.0	41.0
145-149	37.42167008027181	41.0	37.0	41.0	27.0	41.0
150-154	33.73262756893705	35.8	34.0	35.8	31.0	35.8
155-159	38.05342911039121	40.2	39.4	40.2	32.0	40.2
160-164	38.925654621604636	41.0	41.0	41.0	35.0	41.0
165-169	38.940466848807326	41.0	41.0	41.0	36.0	41.0
170-174	38.869104360712036	41.0	41.0	41.0	35.0	41.0
175-179	38.850510569918846	41.0	41.0	41.0	35.0	41.0
180-184	38.72872539538373	41.0	41.0	41.0	33.0	41.0
185-189	38.65897496240132	41.0	41.0	41.0	32.0	41.0
190-194	38.717923081731634	41.0	41.0	41.0	32.0	41.0
195-199	38.715093160164884	41.0	41.0	41.0	32.0	41.0
200-204	38.45470470783339	41.0	40.2	41.0	32.0	41.0
205-209	38.2067692341635	41.0	37.0	41.0	32.0	41.0
210-214	38.34044956628217	41.0	38.6	41.0	32.0	41.0
215-219	38.265977326797845	41.0	37.0	41.0	32.0	41.0
220-224	38.08204455949785	41.0	37.0	41.0	32.0	41.0
225-229	37.88565954896286	41.0	37.0	41.0	31.0	41.0
230-234	38.35377218690131	41.0	38.6	41.0	32.0	41.0
235-239	38.24874885549235	41.0	37.0	41.0	32.0	41.0
240-244	38.07727274398697	41.0	37.0	41.0	32.0	41.0
245-249	38.00453059558524	41.0	37.0	41.0	32.0	41.0
250-254	37.85028773565826	41.0	37.0	41.0	31.0	41.0
255-259	37.590673319825996	41.0	37.0	41.0	27.0	41.0
260-264	37.315049402282035	41.0	37.0	41.0	27.0	41.0
265-269	37.17556839283547	41.0	37.0	41.0	27.0	41.0
270-274	36.892627716022375	41.0	37.0	41.0	27.0	41.0
275-279	36.39783490408199	41.0	37.0	41.0	25.0	41.0
280-284	35.8133899121533	41.0	35.0	41.0	22.0	41.0
285-289	35.613297616114664	41.0	32.0	41.0	22.0	41.0
290-294	35.198594967475756	41.0	32.0	41.0	22.0	41.0
295-299	34.250972417825274	37.0	32.0	41.0	22.0	41.0
300	33.76236344047273	37.0	32.0	41.0	12.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
13	2.0
14	0.0
15	2.0
16	2.0
17	2.0
18	7.0
19	41.0
20	67.0
21	149.0
22	404.0
23	683.0
24	1235.0
25	1851.0
26	2701.0
27	3529.0
28	4683.0
29	6064.0
30	7598.0
31	9240.0
32	11248.0
33	13426.0
34	16703.0
35	20723.0
36	27072.0
37	36697.0
38	56288.0
39	106179.0
40	217306.0
>>END_MODULE
>>Per base sequence content	pass
#Base	G	A	T	C
1	21.64685986681412	30.181538228631453	23.61529329879682	24.556308605757604
2	23.966633695040652	30.734397005342874	26.56140260561645	18.737566694000023
3	20.966972223501774	28.702381500760055	27.9329310424248	22.397715233313374
4	21.57282010362161	27.78037219940357	27.360995179278618	23.285812517696204
5	22.324426091465007	27.71786093818372	27.63769943850179	22.320013531849487
6	22.058385517979342	27.21354214546003	27.99272663090042	22.73534570566021
7	22.6079330467621	27.629058175921394	27.363569172387674	22.39943960492883
8	21.540461333107803	27.753896841710457	28.385260580030963	22.32038124515078
9	21.86368132494457	27.339483951152964	28.4729602023894	22.32387452151307
10-14	22.059120944581927	27.864394688749076	27.900246735625167	22.176237631043826
15-19	22.02242315711286	27.682707546580083	27.973826167213943	22.32104312909311
20-24	22.10898286823729	27.74172553143765	27.819754293972075	22.329537306352982
25-29	22.329574077683112	27.639758632989032	27.69116495250983	22.33950233681803
30-34	22.364132543283752	27.60793771096703	27.645373499212855	22.38255624653636
35-39	22.352923872315234	27.63369136351769	27.674397225970853	22.33898753819622
40-44	22.41014006199646	27.624792701626394	27.63273530893433	22.332331927442812
45-49	22.366548049247864	27.53046918294454	27.674435451813224	22.428547315994376
50-54	22.336965115039106	27.626998981434152	27.62946266055282	22.406573242973916
55-59	22.46259353913475	27.569067274632737	27.602382834764384	22.365956351468128
60-64	22.396406772530668	27.491212934912834	27.62830512426186	22.484075168294638
65-69	22.371081817249106	27.613859387255957	27.58881655518745	22.426242240307488
70-74	22.48934550709503	27.584711951785433	27.502417714956	22.423524826163536
75-79	22.358706276297102	27.558738930487024	27.5482219278769	22.534332865338975
80-84	22.40692296207933	27.662062488462585	27.563916343741703	22.367098205716378
85-89	22.414758039547063	27.748507626786456	27.505521247702696	22.331213085963785
90-94	22.364848267330736	27.5413070882125	27.614117588222054	22.47972705623471
95-99	22.355534717817186	27.683536041626443	27.60664478704138	22.35428445351499
100-104	22.459192416169326	27.58927290979492	27.535659521892132	22.415875152143617
105-109	22.347665572106738	27.589087740070823	27.618173862203115	22.445072825619324
110-114	22.37774452015253	27.55261058058253	27.65031200473615	22.419332894528793
115-119	22.4691212755239	27.592213303131814	27.506315475949712	22.432349945394574
120-124	22.41659661687064	27.53181290342983	27.60200978564395	22.449580694055584
125-129	22.37752389217175	27.593904784317765	27.604825869366174	22.42374545414431
130-134	22.473202893168253	27.6311909130689	27.54977918816257	22.345827005600274
135-139	22.402307768678916	27.574857235310773	27.544667973274596	22.47816702273571
140-144	22.435549051115828	27.52462759835412	27.60990031292402	22.429923037606038
145-149	22.42775352912841	27.642957738710283	27.597876087971727	22.331412644189577
150-154	22.11863131668503	29.07784548618026	26.436959641956786	22.366563555177922
155-159	22.119278840673502	27.559155877345553	27.94360013384764	22.377965148133303
160-164	22.148107563494897	27.729223279193675	27.976510474313386	22.146158682998042
165-169	22.09155325775599	27.63163216903045	28.0384701655813	22.23834440763226
170-174	22.16638291456917	27.7185963647863	27.90458575258043	22.2104349680641
175-179	22.32980468573316	27.650319321340568	27.706582539059728	22.31329345386655
180-184	22.317108596769273	27.626006155520667	27.673698570698395	22.38318667701167
185-189	22.347371401465704	27.604494927395006	27.704954201308325	22.34317946983096
190-194	22.431099720170177	27.641781056146144	27.561656327794342	22.365462895889333
195-199	22.384179502925157	27.545109229236147	27.62714606675467	22.443565201084017
200-204	22.379619857989123	27.629977459174633	27.58533706439763	22.405065618438616
205-209	22.487212769947526	27.569782791752928	27.496975558096864	22.446028880202682
210-214	22.416354416788316	27.51907512750459	27.58794782883681	22.47662262687028
215-219	22.376862008229423	27.58496935109634	27.604642012715523	22.433526627958713
220-224	22.44510959694945	27.5498159594927	27.5510661847171	22.454008258840748
225-229	22.43698313299087	27.554853631720423	27.54948501752154	22.458678217767172
230-234	22.395174130633826	27.590632135936254	27.576879658467885	22.437314074962032
235-239	22.444999282959063	27.590595364606123	27.475280473320563	22.489124879114254
240-244	22.398851263646762	27.50958812433122	27.57147427293887	22.520086339083143
245-249	22.380097885280804	27.563641979621327	27.619129916786477	22.43713021831139
250-254	22.486293486694294	27.56838548120801	27.53521774143136	22.410103290666335
255-259	22.44393291438531	27.5064257899401	27.591036620567678	22.458604675106912
260-264	22.396461127188356	27.55334600718512	27.636191813966487	22.414001051660044
265-269	22.501443274707576	27.572430327522234	27.496938786766734	22.429187611003453
270-274	22.420546348423063	27.54441057396369	27.57106978830745	22.463973289305795
275-279	22.462943692062172	27.563384580310423	27.547278737713782	22.426392989913623
280-284	22.500376906133827	27.526355850870193	27.501167489731603	22.472099753264374
285-289	22.45967104368066	27.456012296332794	27.557354082169216	22.52696257781733
290-294	22.43135711948108	27.52815764604653	27.555956771624302	22.484528462848086
295-299	22.52574912392306	27.486532500340132	27.487451783593368	22.500266592143436
300	22.393188478806845	27.367062448749955	27.616004353725486	22.623744718717713
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.25
13	0.5
14	0.5
15	0.75
16	0.25
17	1.0
18	2.5
19	3.25
20	3.25
21	10.75
22	24.0
23	35.25
24	58.5
25	119.5
26	223.25
27	355.0
28	521.75
29	772.0
30	1104.0
31	1469.25
32	1956.75
33	2499.75
34	3005.75
35	3629.5
36	4329.0
37	5000.75
38	5800.5
39	6536.25
40	7395.0
41	8260.5
42	8976.75
43	9607.75
44	10103.25
45	10502.75
46	10741.0
47	10948.0
48	11011.0
49	10572.5
50	9857.75
51	8771.5
52	7502.25
53	5910.0
54	4307.25
55	2895.75
56	1837.25
57	1141.25
58	695.75
59	475.0
60	370.25
61	317.75
62	326.0
63	315.5
64	276.5
65	233.75
66	175.25
67	131.0
68	92.0
69	59.5
70	35.75
71	20.75
72	12.5
73	7.5
74	2.5
75	1.75
76	1.5
77	1.0
78	1.75
79	1.0
80	0.5
81	0.75
82	0.5
83	0.5
84	0.25
85	0.0
86	0.25
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.25
100	0.6666666666666666
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.41422903390684357
2	0.0
3	0.21639927781107623
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.006876238734183732
35-39	0.0
40-44	0.0
45-49	0.004522873605906946
50-54	0.0
55-59	0.0022062798077594863
60-64	0.006324668782243859
65-69	0.0062143547918558865
70-74	0.0
75-79	0.003824218333449776
80-84	0.003530047692415178
85-89	5.883412820691963E-4
90-94	0.004486102275777622
95-99	0.003125563060992605
100-104	0.0014708532051729906
105-109	0.0
110-114	0.0
115-119	0.0
120-124	5.883412820691963E-4
125-129	0.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150-154	0.2898316240793378
155-159	0.0
160-164	0.0
165-169	0.0
170-174	0.0
175-179	0.005478928189269391
180-184	0.0
185-189	0.0
190-194	0.0
195-199	0.0
200-204	0.0
205-209	0.0
210-214	0.0
215-219	0.0
220-224	0.0
225-229	0.0
230-234	0.0
235-239	0.0
240-244	0.0
245-249	0.0
250-254	0.0
255-259	0.0
260-264	0.0
265-269	0.0
270-274	0.0
275-279	0.0
280-284	0.0
285-289	0.0
290-294	0.0
295-299	0.0
300	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
300	543902.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	93.74473649940582
#Duplication Level	Percentage of total
1	88.1603153429469
2	10.05283933638598
3	1.4254566620254114
4	0.2512546450413045
5	0.07669969400732392
6	0.017357956088784154
7	0.0017145984639379138
8	0.005569947881902913
9	0.0040076058503955075
>10	0.004784211308058733
>50	0.0
>100	0.0
>500	0.0
>1k	0.0
>5k	0.0
>10k+	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	0.0	0.0	0.0	0.0	3.6771330129324766E-4	0.0
2	0.0	0.0	0.0	0.0	9.192832532331192E-4	0.0
3	0.0	0.0	0.0	0.0	0.0011031399038797431	0.0
4	0.0	0.0	0.0	0.0	0.0014708532051729906	0.0
5	0.0	0.0	0.0	0.0	0.0016547098558196145	0.0
6	0.0	0.0	0.0	0.0	0.0022062798077594863	0.0
7	0.0	0.0	0.0	0.0	0.00239013645840611	0.0
8	0.0	0.0	0.0	0.0	0.00239013645840611	0.0
9	0.0	0.0	0.0	0.0	0.00239013645840611	0.0
10-14	0.0	0.0	0.0	0.0	0.00308879173086328	0.0
15-19	0.0	0.0	0.0	0.0	0.004228702964872349	0.0
20-24	0.0	0.0	0.0	0.0	0.0051112148879761425	0.0
25-29	0.0	0.0	0.0	0.0	0.006251126121985209	0.0
30-34	0.0	0.0	0.0	0.0	0.006434982772631834	0.0
35-39	0.0	0.0	0.0	0.0	0.006802696073925081	3.677133012932477E-5
40-44	0.0	0.0	0.0	0.0	0.007685207997028876	1.8385665064662383E-4
45-49	0.0	0.0	0.0	0.0	0.008494177259874022	1.8385665064662383E-4
50-54	0.0	0.0	0.0	0.0	0.009891487804788364	1.8385665064662383E-4
55-59	0.0	0.0	0.0	0.0	0.010295972436210936	1.8385665064662383E-4
60-64	0.0	0.0	0.0	3.677133012932477E-5	0.010590143077245533	1.8385665064662383E-4
65-69	0.0	0.0	0.0	1.8385665064662383E-4	0.011472655000349326	1.8385665064662383E-4
70-74	0.0	0.0	0.0	1.8385665064662383E-4	0.012796422885005019	1.8385665064662383E-4
75-79	0.0	0.0	0.0	1.8385665064662383E-4	0.013862791458755436	1.8385665064662383E-4
80-84	0.0	0.0	0.0	1.8385665064662383E-4	0.014598218061341933	2.206279807759486E-4
85-89	0.0	0.0	0.0	1.8385665064662383E-4	0.015591043974833702	3.6771330129324766E-4
90-94	0.0	0.0	0.0	1.8385665064662383E-4	0.016694183878713444	4.4125596155189723E-4
95-99	0.0	0.0	0.0	1.8385665064662383E-4	0.01765023846207589	5.515699519398716E-4
100-104	0.0	0.0	0.0	1.8385665064662383E-4	0.01820180841401576	5.515699519398716E-4
105-109	0.0	0.0	0.0	1.8385665064662383E-4	0.01875337836595563	5.515699519398716E-4
110-114	0.0	0.0	0.0	1.8385665064662383E-4	0.019304948317895504	5.515699519398716E-4
115-119	0.0	0.0	0.0	2.206279807759486E-4	0.019782975609576724	5.515699519398716E-4
120-124	0.0	0.0	0.0	3.6771330129324766E-4	0.0203345455615166	5.515699519398716E-4
125-129	0.0	0.0	0.0	3.6771330129324766E-4	0.02114351482436174	5.515699519398716E-4
130-134	0.0	0.0	0.0	3.6771330129324766E-4	0.022393740048758784	7.354266025864953E-4
135-139	0.0	0.0	0.0	3.6771330129324766E-4	0.023717507933414476	7.354266025864953E-4
140-144	0.0	1.4708532051729907E-4	0.0	4.4125596155189723E-4	0.024269077885354345	7.354266025864953E-4
145-149	0.0	1.8385665064662383E-4	0.0	7.721979327158202E-4	0.024526477196259618	7.354266025864953E-4
150-154	0.0	1.8385665064662383E-4	0.0	9.192832532331191E-4	0.02537221778923409	7.354266025864953E-4
155-159	0.0	1.8385665064662383E-4	0.0	9.192832532331191E-4	0.026732757004019107	7.354266025864953E-4
160-164	0.0	1.8385665064662383E-4	0.0	9.192832532331191E-4	0.027946210898286823	7.354266025864953E-4
165-169	0.0	1.8385665064662383E-4	0.0	9.192832532331191E-4	0.02941706410345981	7.354266025864953E-4
170-174	0.0	1.8385665064662383E-4	0.0	9.192832532331191E-4	0.030262804696434286	7.354266025864953E-4
175-179	0.0	1.8385665064662383E-4	0.0	9.192832532331191E-4	0.03059374666759821	7.354266025864953E-4
180-184	0.0	1.8385665064662383E-4	0.0	9.192832532331191E-4	0.031145316619538076	7.354266025864953E-4
185-189	1.8385665064662383E-4	1.8385665064662383E-4	0.0	9.192832532331191E-4	0.03243231317406444	8.825119231037944E-4
190-194	2.9417064103459814E-4	1.8385665064662383E-4	0.0	9.192832532331191E-4	0.03390316637923744	9.192832532331191E-4
195-199	5.515699519398716E-4	1.8385665064662383E-4	0.0	9.192832532331191E-4	0.035006306283117176	9.192832532331191E-4
200-204	5.515699519398716E-4	1.8385665064662383E-4	0.0	9.192832532331191E-4	0.036146217517126246	9.192832532331191E-4
205-209	6.986552724571706E-4	1.8385665064662383E-4	0.0	9.192832532331191E-4	0.03717581476074734	9.192832532331191E-4
210-214	7.721979327158201E-4	1.8385665064662383E-4	0.0	9.192832532331191E-4	0.03761707072229924	9.192832532331191E-4
215-219	0.0010663685737504183	1.8385665064662383E-4	0.0	9.192832532331191E-4	0.038315725994756404	9.192832532331191E-4
220-224	0.0011031399038797431	1.8385665064662383E-4	0.0	9.192832532331191E-4	0.03883052461656696	9.192832532331191E-4
225-229	0.0013973105449143412	2.573993109052734E-4	0.0	9.192832532331191E-4	0.03941886589863615	0.0010295972436210935
230-234	0.0016179385256902897	3.6771330129324766E-4	0.0	9.192832532331191E-4	0.04041169181212792	0.0011031399038797431
235-239	0.0016547098558196145	3.6771330129324766E-4	0.0	9.192832532331191E-4	0.041183889744843744	0.0011031399038797431
240-244	0.0016547098558196145	3.6771330129324766E-4	0.0	9.192832532331191E-4	0.04213994432820618	0.001286996554526367
245-249	0.0016547098558196145	3.6771330129324766E-4	0.0	9.192832532331191E-4	0.04291214226092201	0.0013973105449143412
250-254	0.001728252516078264	3.6771330129324766E-4	0.0	9.192832532331191E-4	0.043463712212861876	0.001581167195560965
255-259	0.0018385665064662381	3.6771330129324766E-4	0.0	9.192832532331191E-4	0.04423591014557769	0.0016547098558196145
260-264	0.0018385665064662381	3.6771330129324766E-4	0.0	9.192832532331191E-4	0.045155193398810814	0.001728252516078264
265-269	0.0020224231571128624	3.6771330129324766E-4	0.0	9.192832532331191E-4	0.04585384867126798	0.0019488804968542128
270-274	0.0020224231571128624	3.6771330129324766E-4	0.0	9.192832532331191E-4	0.04651573261359583	0.002059194487242187
275-279	0.0020224231571128624	3.6771330129324766E-4	0.0	9.192832532331191E-4	0.047508558527087595	0.00239013645840611
280-284	0.0022798224680181355	3.6771330129324766E-4	0.0	9.192832532331191E-4	0.04846461311045004	0.00239013645840611
285-289	0.00239013645840611	3.6771330129324766E-4	0.0	9.192832532331191E-4	0.049420667693812495	0.00239013645840611
>>END_MODULE
