FastQCFastQC Report
Wed 13 Sep 2023
stdin:SRR7516710

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR7516710
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5130780
Total Bases1.5 Gbp
Sequences flagged as poor quality0
Sequence length300
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGT756571.4745711178417318No Hit
ATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTT342750.6680270836013238RNA PCR Primer, Index 26 (100% over 22bp)
ACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTTCT219580.4279661182120457RNA PCR Primer, Index 26 (100% over 24bp)
TCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTTCTGCTTG152760.2977325085074784RNA PCR Primer, Index 26 (100% over 29bp)
TTATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTC143650.27997692358666715RNA PCR Primer, Index 26 (95% over 22bp)
CATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTTCTGCT88230.17196215780056834RNA PCR Primer, Index 26 (100% over 27bp)
TATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCT73320.14290224878088711RNA PCR Primer, Index 26 (100% over 21bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)