FastQCFastQC Report
Wed 13 Sep 2023
stdin:SRR7516711

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR7516711
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6762008
Total Bases2 Gbp
Sequences flagged as poor quality0
Sequence length300
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGT477240.7057666894212489No Hit
ATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTT243600.36024802100204556RNA PCR Primer, Index 17 (95% over 21bp)
ACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTTCT139930.20693557298364626RNA PCR Primer, Index 17 (95% over 23bp)
TCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTTCTGCTTG79590.11770172410325452RNA PCR Primer, Index 17 (96% over 28bp)
TTATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTC75540.1117123789264964No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)