FastQCFastQC Report
Wed 13 Sep 2023
stdin:SRR7516714

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR7516714
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16230302
Total Bases4.8 Gbp
Sequences flagged as poor quality0
Sequence length300
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGT1229970.757823237053753No Hit
ATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTT661630.40765107143416063TruSeq Adapter, Index 11 (95% over 21bp)
ACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCT397940.24518336134472424RNA PCR Primer, Index 11 (95% over 23bp)
TCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCTGCTTG300980.1854432529967711TruSeq Adapter, Index 11 (96% over 28bp)
TTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTC245800.15144511790353624No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)