FastQCFastQC Report
Wed 13 Sep 2023
stdin:SRR7516717

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR7516717
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences581758
Total Bases174.5 Mbp
Sequences flagged as poor quality0
Sequence length300
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGT26830.4611883291677983No Hit
ATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTT13900.23893096442163236RNA PCR Primer, Index 33 (95% over 22bp)
ACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTTCT8480.14576507757521168RNA PCR Primer, Index 33 (95% over 24bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)