##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	SRR7516717
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	581758
Total Bases	174.5 Mbp
Sequences flagged as poor quality	0
Sequence length	300
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.603941845234615	32.0	32.0	32.0	32.0	32.0
2	31.3699871767986	32.0	32.0	32.0	32.0	32.0
3	35.43385050141124	37.0	37.0	37.0	32.0	37.0
4	35.98827347453752	37.0	37.0	37.0	32.0	37.0
5	36.25093423726017	37.0	37.0	37.0	37.0	37.0
6	39.73945351847332	41.0	41.0	41.0	37.0	41.0
7	40.00066866291482	41.0	41.0	41.0	37.0	41.0
8	40.0376273295769	41.0	41.0	41.0	37.0	41.0
9	40.02500352380199	41.0	41.0	41.0	37.0	41.0
10-14	40.00972603728698	41.0	41.0	41.0	37.0	41.0
15-19	40.020647416967186	41.0	41.0	41.0	37.0	41.0
20-24	39.97324454498262	41.0	41.0	41.0	37.0	41.0
25-29	39.871973913551685	41.0	41.0	41.0	37.0	41.0
30-34	39.83521567387127	41.0	41.0	41.0	37.0	41.0
35-39	39.734025832047	41.0	41.0	41.0	37.0	41.0
40-44	39.71044661182141	41.0	41.0	41.0	37.0	41.0
45-49	39.6371924408431	41.0	41.0	41.0	37.0	41.0
50-54	39.562416331189254	41.0	41.0	41.0	37.0	41.0
55-59	39.52311854757477	41.0	41.0	41.0	37.0	41.0
60-64	39.341549579034584	41.0	41.0	41.0	37.0	41.0
65-69	39.27836729361694	41.0	41.0	41.0	37.0	41.0
70-74	39.03199921616893	41.0	41.0	41.0	37.0	41.0
75-79	38.62550063772221	41.0	40.2	41.0	34.0	41.0
80-84	38.93702811134526	41.0	41.0	41.0	36.0	41.0
85-89	38.91573128345463	41.0	41.0	41.0	36.0	41.0
90-94	38.774854836547156	41.0	41.0	41.0	32.0	41.0
95-99	38.66415829262339	41.0	41.0	41.0	32.0	41.0
100-104	38.50027709116162	41.0	41.0	41.0	32.0	41.0
105-109	38.398311669113276	41.0	41.0	41.0	32.0	41.0
110-114	38.18425221483847	41.0	41.0	41.0	32.0	41.0
115-119	37.650312672967104	41.0	37.0	41.0	27.0	41.0
120-124	37.30772039232808	41.0	37.0	41.0	26.0	41.0
125-129	36.82658768766394	41.0	37.0	41.0	22.0	41.0
130-134	36.2891356887228	41.0	37.0	41.0	22.0	41.0
135-139	35.59847290454106	41.0	35.0	41.0	16.0	41.0
140-144	34.849026571185945	41.0	32.0	41.0	12.0	41.0
145-149	34.08861175952888	41.0	32.0	41.0	12.0	41.0
150-154	32.31786137878637	35.0	31.0	35.8	28.0	35.8
155-159	37.831636522402775	40.2	39.4	40.2	33.0	40.2
160-164	38.60388958982945	41.0	41.0	41.0	32.0	41.0
165-169	38.666635955156615	41.0	41.0	41.0	33.0	41.0
170-174	38.58826728639743	41.0	41.0	41.0	32.0	41.0
175-179	38.50510521557074	41.0	41.0	41.0	32.0	41.0
180-184	38.444617521374866	41.0	41.0	41.0	32.0	41.0
185-189	38.38311565977606	41.0	41.0	41.0	32.0	41.0
190-194	38.29433200746702	41.0	41.0	41.0	32.0	41.0
195-199	38.20318173536075	41.0	41.0	41.0	32.0	41.0
200-204	38.06138566207942	41.0	41.0	41.0	30.0	41.0
205-209	37.89056824315266	41.0	41.0	41.0	27.0	41.0
210-214	37.78066756280102	41.0	41.0	41.0	27.0	41.0
215-219	37.64548145448795	41.0	37.0	41.0	27.0	41.0
220-224	37.52583170321681	41.0	37.0	41.0	27.0	41.0
225-229	37.096358967130655	41.0	37.0	41.0	26.0	41.0
230-234	37.511717930823465	41.0	37.0	41.0	27.0	41.0
235-239	37.39556310355852	41.0	37.0	41.0	27.0	41.0
240-244	37.24075715331805	41.0	37.0	41.0	25.0	41.0
245-249	36.8691528092437	41.0	37.0	41.0	22.0	41.0
250-254	36.711697991260976	41.0	37.0	41.0	22.0	41.0
255-259	36.37327995489533	41.0	37.0	41.0	22.0	41.0
260-264	35.92091110049195	41.0	37.0	41.0	22.0	41.0
265-269	35.31276716435356	41.0	34.0	41.0	12.0	41.0
270-274	34.55915243107959	41.0	32.0	41.0	12.0	41.0
275-279	33.884180019870804	41.0	32.0	41.0	12.0	41.0
280-284	33.19493878898099	40.2	27.0	41.0	12.0	41.0
285-289	31.96015869141464	37.0	26.0	41.0	12.0	41.0
290-294	31.340715555265245	37.0	22.0	41.0	12.0	41.0
295-299	30.406604120613725	37.0	22.0	41.0	12.0	41.0
300	26.116780516984726	27.0	12.0	37.0	12.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
13	3.0
14	3.0
15	2.0
16	4.0
17	18.0
18	277.0
19	2815.0
20	2804.0
21	1232.0
22	1194.0
23	1591.0
24	2189.0
25	3134.0
26	4120.0
27	5348.0
28	6786.0
29	8383.0
30	10063.0
31	12476.0
32	15429.0
33	19274.0
34	25319.0
35	29943.0
36	35078.0
37	43477.0
38	57289.0
39	95721.0
40	197786.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.807043841795476	21.143902304857253	13.445974751086418	24.60307910226085
2	14.368720138551938	20.670381414977214	42.20256832195714	22.758330124513712
3	18.178520965762395	25.79491816184737	29.980335465949757	26.046225406440477
4	11.839287126262123	17.665592909766602	40.13507334664929	30.360046617321977
5	13.2788891600975	37.16889153221786	36.413766548977414	13.138452758707228
6	29.638440726212618	39.18914737743185	18.490678254531954	12.681733641823575
7	25.784260809477484	31.757363027238128	24.184110918973182	18.27426524431121
8	22.869646829093885	37.951175574723514	22.16798050048302	17.011197095699586
9	22.725428786540107	17.98754808700525	22.496123817807405	36.79089930864724
10-14	22.45799799916804	25.30811780843581	29.884934972961265	22.348949219434886
15-19	20.872493373533324	30.143633607101233	27.465440956548942	21.518432062816498
20-24	21.038301149275128	29.14964641655121	28.69289292111153	21.119159513062133
25-29	20.85933798913219	29.223892952838547	28.728667129175385	21.188101928853875
30-34	21.023196267035022	29.06259550802448	28.981049489013806	20.933158735926693
35-39	20.67622619714727	28.983563612361152	29.15617834219727	21.184031848294307
40-44	20.668697293376283	28.46200653880136	29.66738747039147	21.20190869743089
45-49	20.703719276917475	28.410855658197427	29.582999123634266	21.302425941250835
50-54	20.300846743835066	28.382076396027212	29.885794436862064	21.431282423275658
55-59	20.249240033802252	28.50871975692083	29.629536676749126	21.61250353252779
60-64	20.181656290072507	29.285819876993525	28.90143324200097	21.631090590933
65-69	20.04205396053969	29.233691025783838	28.8260440260135	21.89821098766298
70-74	20.309028840170658	29.095122026684635	28.553315983622056	22.042533149522654
75-79	20.523274108489275	29.079576112206805	28.263370296559824	22.133779482744096
80-84	20.411992615486163	28.91676607799119	28.302833824373707	22.368407482148935
85-89	20.528271712872982	28.74879447953342	28.2361364716261	22.486797335967502
90-94	20.414091717477927	28.49363237359309	28.314411783719397	22.777864125209586
95-99	20.40486412158171	28.220492074834013	28.440944242596466	22.93369956098781
100-104	20.37054891263552	28.452973612498962	28.33318067969663	22.84329679516889
105-109	20.184047094687116	28.762125980077975	28.216917269681247	22.836909655553665
110-114	20.03401946830131	29.518666497395042	27.870194810310846	22.57711922399281
115-119	19.924971762919302	29.861603040487655	27.76350967560815	22.449915520984888
120-124	19.86504494147157	30.174113822390424	27.50272289209646	22.45811834404154
125-129	19.701130259143067	30.41791224375779	27.4076926382741	22.473264858825047
130-134	19.75524824153223	30.568011392821997	27.150749287089077	22.525991078556697
135-139	19.779599077279556	30.573159286163666	26.945121511006292	22.702120125550486
140-144	19.793075471244055	30.822266303170736	26.64399973872297	22.740658486862234
145-149	19.668900126856872	30.808824287762267	26.548599245734483	22.97367633964638
150-154	21.136740731666865	23.358174597221236	30.07880431603483	25.42628035507707
155-159	23.032532427572978	32.59224626047257	24.825889802976494	19.54933150897796
160-164	22.670877812037933	25.374339630684794	29.70927076525661	22.245511792020668
165-169	20.968512680530395	30.048061221332585	27.528113064195075	21.455313033941948
170-174	21.154225640214662	28.982979176908614	28.69052080074533	21.172274382131402
175-179	20.941837435251642	28.76613100904474	28.73711521847275	21.55491633723087
180-184	20.824498001908704	28.90927317882161	29.000136139737126	21.26609267953256
185-189	20.748221908072782	28.8983943541112	28.999709839600428	21.353673898215582
190-194	20.595745997476612	28.728130941044217	29.121318486380936	21.554804575098238
195-199	20.626549183681185	28.857256797499993	29.053661488110176	21.462532530708643
200-204	20.59968756317151	28.64417955766536	29.015817114442367	21.740315764720766
205-209	20.51031528573737	28.45007717985829	29.037503566775186	22.002103967629154
210-214	20.64115200673283	28.498043649932807	28.88281598895464	21.977988354379725
215-219	20.644872954046185	28.358733356481565	28.895588887475547	22.100804801996706
220-224	20.89642907360951	28.251845042236486	28.838559362968375	22.013166521185624
225-229	21.033797189360453	28.328210255117952	28.676712506785602	21.961280048735997
230-234	21.10492679086493	27.98173811103586	28.917212999219604	21.996122098879603
235-239	21.331481475115083	27.904111331515853	28.76625675968358	21.998150433685485
240-244	21.292859979131894	27.725187323055135	28.81675774260033	22.165194955212645
245-249	21.280944287219093	27.61646135694189	28.81789453708364	22.284699818755378
250-254	21.20668731671932	27.95808566448592	28.66329298436807	22.171934034426684
255-259	20.951780139990923	28.031051032054897	28.773979290144254	22.243189537809926
260-264	20.981669766978072	28.57449859196578	28.46656649394322	21.977265147112927
265-269	20.82139948424457	28.878075512343305	28.37156806221794	21.928956941194187
270-274	20.7024370186736	29.229028599481772	28.160875479886784	21.907658901957845
275-279	20.625002148659753	29.566177001433587	28.072978798744497	21.735842051162166
280-284	20.59041633190214	29.818228448469984	27.756766540805515	21.83458867882236
285-289	20.428150536821153	29.85588509311432	27.696705502975465	22.019258867089064
290-294	20.35530998520344	30.220841423770207	27.41231387411372	22.011534716912635
295-299	20.262377139635383	30.214178404078673	27.373443940607604	22.15000051567834
300	20.014335857865298	30.52557936461553	26.84157330023824	22.61851147728093
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.25
5	0.25
6	0.0
7	0.0
8	0.5
9	0.0
10	2.75
11	6.25
12	5.0
13	4.25
14	3.5
15	5.75
16	6.75
17	8.5
18	16.25
19	29.5
20	52.25
21	80.0
22	118.5
23	193.25
24	289.75
25	443.75
26	658.75
27	962.0
28	1346.75
29	1799.5
30	2364.75
31	3042.25
32	3825.5
33	4639.25
34	5484.0
35	6438.75
36	7465.0
37	8391.25
38	9400.5
39	10136.25
40	10738.0
41	10713.0
42	10543.0
43	9897.25
44	9327.0
45	8723.75
46	7942.0
47	7402.25
48	6923.25
49	6387.0
50	5847.0
51	5270.75
52	4667.25
53	4087.25
54	3454.0
55	2946.0
56	2435.0
57	1947.75
58	1583.5
59	1242.5
60	1008.75
61	827.0
62	634.75
63	499.75
64	374.75
65	310.0
66	243.25
67	178.0
68	134.5
69	101.75
70	76.25
71	60.25
72	48.0
73	29.5
74	26.75
75	22.25
76	19.5
77	16.25
78	12.25
79	8.25
80	5.0
81	5.0
82	3.25
83	0.75
84	1.25
85	1.75
86	2.25
87	2.5
88	0.75
89	0.25
90	0.5
91	0.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.25
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.005156783404783432
2	0.4512185479185503
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	5.500568965102327E-4
30-34	1.0313566809566864E-4
35-39	0.0
40-44	0.0
45-49	0.006428789977963346
50-54	0.0
55-59	0.003712884051444071
60-64	0.0
65-69	0.005019269180655874
70-74	0.0
75-79	0.03248773545013562
80-84	0.0
85-89	0.08144279923954634
90-94	0.022071032972473092
95-99	0.07002911863695901
100-104	0.1585539004190746
105-109	0.12596302930084333
110-114	0.07628601583476291
115-119	0.07394827402459442
120-124	0.004056669611762967
125-129	0.005672461745261776
130-134	0.010038538361311748
135-139	0.0
140-144	0.0
145-149	0.0
150-154	0.0895217599070404
155-159	0.0
160-164	3.4378556031889546E-5
165-169	0.0
170-174	0.0
175-179	9.96978124924797E-4
180-184	4.8129978444645373E-4
185-189	0.0018564420257220354
190-194	0.0
195-199	0.0
200-204	0.001443899353339361
205-209	0.0
210-214	0.0019595776938177044
215-219	0.0
220-224	0.0016845492455625879
225-229	0.0019595776938177044
230-234	0.0
235-239	0.0
240-244	0.0015470350214350297
245-249	4.8129978444645373E-4
250-254	0.0
255-259	0.0010313566809566864
260-264	0.016501706895306983
265-269	0.0016157921334988088
270-274	0.001065735236988576
275-279	0.0
280-284	9.96978124924797E-4
285-289	0.0
290-294	2.0627133619133728E-4
295-299	0.0
300	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
300	581758.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	95.00989125906703
#Duplication Level	Percentage of total
1	91.86739497819416
2	5.6749016908542
3	0.5667399885780738
4	0.18964625898484874
5	0.12364752368985174
6	0.0884754229135314
7	0.0577829711224266
8	0.03740819806892105
9	0.03257821890431162
>10	0.1738681206036969
>50	0.021524019172918338
>100	0.29066320106238486
>500	0.1508460186664029
>1k	0.7245233891842676
>5k	0.0
>10k+	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGT	2683	0.4611883291677983	No Hit
ATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTT	1390	0.23893096442163236	RNA PCR Primer, Index 33 (95% over 22bp)
ACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTTCT	848	0.14576507757521168	RNA PCR Primer, Index 33 (95% over 24bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	0.0	0.0	0.0	0.0	8.594639007972388E-4	0.0
2	0.0	0.0	0.0	0.0	0.0010313566809566864	0.0
3	0.0	0.0	0.0	0.0	0.0010313566809566864	0.0
4	0.0	0.0	0.0	0.0	0.001890820581753925	0.0
5	0.0	0.0	0.0	0.0	0.002578391702391716	0.0
6	0.0	0.0	0.0	0.0	0.004297319503986194	0.0
7	0.0	0.0	0.0	0.0	0.004812997844464537	0.0
8	0.0	0.0	0.0	0.0	0.004984890624623984	0.0
9	0.0	0.0	0.0	0.0	0.005156783404783432	0.0
10-14	0.0	0.0	0.0	4.1254267238267456E-4	0.011310544934491662	0.0
15-19	0.0	0.0	0.0	7.907067887334597E-4	0.034550448812048995	0.0
20-24	0.0	0.0	0.0	0.0014782779093712507	0.06136572251692285	0.0
25-29	0.0	0.0	0.0	0.009694752800992853	0.0848806548427353	2.0627133619133728E-4
30-34	0.0	0.0	0.0	0.14882476906204983	0.1059547096902836	3.437855603188955E-4
35-39	0.0	0.0	0.0	0.5691026165518996	0.13603594621818696	3.437855603188955E-4
40-44	0.0	0.0	0.0	1.4350296858831335	0.16546399018148442	4.812997844464537E-4
45-49	0.0	0.0	0.0	2.4093523423829155	0.19375754179572952	6.87571120637791E-4
50-54	0.0	0.0	0.0	3.5049969231192355	0.31098841786447284	7.563282327015702E-4
55-59	0.0	0.0	0.0	4.656231628959121	0.6161324812035245	0.0011688709050842446
60-64	0.0	0.0	0.0	5.871857370246735	1.254576645271745	0.0012032494611161342
65-69	0.0	0.0	0.0	7.108625923493962	1.3646911602418874	0.001443899353339361
70-74	0.0	0.0	0.0	8.431409623932975	1.3804709174605247	0.0017876849136582566
75-79	0.0	0.0	0.0	9.764438134069493	1.4010292939675948	0.002337741810168489
80-84	0.0	0.0	0.0	11.16062005163659	1.4175653794189338	0.0025096345903279373
85-89	0.0	0.0	0.0	12.579285544848545	1.4312824232756576	0.002750284482551164
90-94	0.0	0.0	0.0	14.003039064353217	1.4472684518304861	0.0029221772627106117
95-99	0.0	0.0	0.0	15.438412535796672	1.5464849645385195	0.0029221772627106117
100-104	0.0	0.0	0.0	16.899741817044198	1.857920303631407	0.0034722341592208447
105-109	0.0	0.0	0.0	18.325007993014275	2.557489540324327	0.003712884051444071
110-114	0.0	0.0	0.0	19.76887296779761	3.3858752264687375	0.0037816411635078497
115-119	0.0	0.0	0.0	21.181728485040175	4.320525029307719	0.004056669611762967
120-124	0.0	0.0	0.0	22.5875363982962	5.287937596045091	0.0043316980600180835
125-129	0.0	0.0	0.0	23.989150127716336	6.309530767088721	0.004572347952241309
130-134	0.0	0.0	0.0	25.37745935595213	7.338102785006825	0.005466190409070438
135-139	0.0	0.0	0.0	26.7909680657593	8.41387656035671	0.006016247305580671
140-144	0.0	0.0	0.0	27.420164398254947	8.924466874542336	0.006703818426218462
145-149	0.0	0.0	0.0	27.42532118165973	8.926804616352504	0.006703818426218462
150-154	0.0	0.0	0.0	27.425424317327828	8.933577191890786	0.006944468318441689
155-159	0.0	0.0	0.0	27.425664967220047	8.939146517967952	0.007357010990824364
160-164	0.0	0.0	0.0	27.4259399956683	8.951350905359273	0.007735175107175148
165-169	0.0	0.0	0.0	27.426180645560528	8.997727577446293	0.008663396120036167
170-174	0.0	0.0	0.0	27.42666194534497	9.049982982614765	0.009247831572578288
175-179	0.0	0.0	0.0	27.428002709030217	9.093850020111455	0.009488481464801515
180-184	0.0	0.0	0.0	27.431509321745466	9.133350980992097	0.0098666455811523
185-189	0.0	0.0	0.0	27.438763197068198	9.189731812884395	0.010279188253534976
190-194	0.0	0.0	0.0	27.451070720127614	9.252919598871008	0.010691730925917648
195-199	0.0	0.0	0.0	27.467916212583237	9.31407905005174	0.011310544934491662
200-204	0.0	0.0	0.0	27.484864840706962	9.358668037225101	0.012926337067990467
205-209	0.0	0.0	0.0	27.504976295985614	9.422715287112512	0.01375142241275582
210-214	0.0	0.0	0.0	27.526187865057288	9.483187167172604	0.015539107326414076
215-219	0.0	0.0	0.0	27.549015226262462	9.56205157470976	0.019595776938177042
220-224	0.0	0.0	0.0	27.57366465093733	9.600314907573253	0.02323990387755733
225-229	0.0	0.0	0.0	27.59742023315537	9.630946200997666	0.027055923597097076
230-234	0.0	0.0	0.0	27.62585129899374	9.665702921145906	0.03427542036379387
235-239	0.0	0.0	0.0	27.653388522375288	9.700253369957954	0.0378164116350785
240-244	0.0	0.0	0.0	27.67872551817079	9.733703704976984	0.038985282540162744
245-249	0.0	0.0	0.0	27.711625796293305	9.7658476548668	0.039775989328896214
250-254	0.0	0.0	0.0	27.741638275709143	9.818996902492103	0.040188532001278886
255-259	0.0	0.0	0.0	27.77137572667673	9.903155607658167	0.041357402906363125
260-264	0.0	0.0	0.0	27.80431038335528	10.044279580169073	0.04266378803557493
265-269	0.0	0.0	0.0	27.839032724947486	10.199808167657341	0.04369514471653162
270-274	0.0	0.0	0.0	27.87475204466462	10.37806098068269	0.045276558293998535
275-279	0.0	0.0	0.0	27.91002444315334	10.572471715043024	0.047958085664485915
280-284	0.0	0.0	0.0	27.951760010176052	10.786443847785504	0.04978014913417606
285-289	0.0	0.0	0.0	27.988716957910334	11.006088442273247	0.05139594126767488
>>END_MODULE
