FastQCFastQC Report
Wed 13 Sep 2023
stdin:SRR7516721

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR7516721
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17719384
Total Bases5.3 Gbp
Sequences flagged as poor quality0
Sequence length300
%GC44

[WARN]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGT832080.46958743035310935No Hit
ATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTT430030.2426890235010427TruSeq Adapter, Index 10 (95% over 21bp)
ACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTTCT233010.13150005666111192RNA PCR Primer, Index 10 (95% over 23bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)