##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	SRR15674129
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	50880924
Total Bases	5 Gbp
Sequences flagged as poor quality	0
Sequence length	61-100
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	35.232644477918676	37.0	36.0	37.0	31.0	37.0
2	34.75563635597498	37.0	35.0	37.0	29.0	37.0
3	34.903913458804325	37.0	35.0	37.0	29.0	37.0
4	34.89901008873188	37.0	35.0	37.0	29.0	37.0
5	34.85861449764552	37.0	35.0	37.0	29.0	37.0
6	34.747235054143275	37.0	35.0	37.0	29.0	37.0
7	34.86871663336932	37.0	35.0	37.0	29.0	37.0
8	34.84668362154744	37.0	35.0	37.0	29.0	37.0
9	34.821730949697375	37.0	35.0	37.0	29.0	37.0
10-11	34.807745000857295	37.0	35.0	37.0	29.0	37.0
12-13	34.7189503575053	37.0	35.0	37.0	29.0	37.0
14-15	34.73426495753104	37.0	35.0	37.0	29.0	37.0
16-17	34.66904756879022	37.0	35.0	37.0	29.0	37.0
18-19	34.62842780724658	37.0	35.0	37.0	28.5	37.0
20-21	34.601832496202306	37.0	35.0	37.0	28.0	37.0
22-23	34.48670520409574	37.0	35.0	37.0	28.0	37.0
24-25	34.46764222481494	37.0	35.0	37.0	28.0	37.0
26-27	34.433140286524676	37.0	35.0	37.0	28.0	37.0
28-29	34.38795748874372	37.0	35.0	37.0	27.5	37.0
30-31	34.308830211102304	37.0	34.5	37.0	27.0	37.0
32-33	34.25259999209134	37.0	34.0	37.0	27.0	37.0
34-35	34.21541176217633	37.0	34.0	37.0	27.0	37.0
36-37	34.15435588394582	37.0	34.0	37.0	27.0	37.0
38-39	34.1232686340366	37.0	34.0	37.0	27.0	37.0
40-41	34.04370635053719	37.0	34.0	37.0	26.0	37.0
42-43	33.99069519649447	37.0	34.0	37.0	26.0	37.0
44-45	33.90067928994371	37.0	34.0	37.0	26.0	37.0
46-47	33.80850131573868	37.0	34.0	37.0	25.5	37.0
48-49	33.85271756267634	37.0	34.0	37.0	26.0	37.0
50-51	33.755735469741076	37.0	34.0	37.0	25.0	37.0
52-53	33.64498522078726	37.0	33.5	37.0	25.0	37.0
54-55	33.55706495227956	37.0	33.0	37.0	24.5	37.0
56-57	33.49938608622752	37.0	33.0	37.0	24.0	37.0
58-59	33.39561191931185	37.0	33.0	37.0	24.0	37.0
60-61	33.30369810108009	37.0	33.0	37.0	23.5	37.0
62-63	33.21654787182227	37.0	33.0	37.0	23.0	37.0
64-65	33.185285662306256	37.0	33.0	37.0	23.0	37.0
66-67	33.105748523493595	37.0	33.0	37.0	23.0	37.0
68-69	33.05262194143547	37.0	32.0	37.0	22.5	37.0
70-71	32.91676093512052	36.0	32.0	37.0	22.0	37.0
72-73	32.80253195703964	36.0	32.0	37.0	21.5	37.0
74-75	32.67845142026283	36.0	32.0	37.0	21.0	37.0
76-77	32.68779742966632	36.0	32.0	37.0	21.0	37.0
78-79	32.62077454318366	36.0	32.0	37.0	21.0	37.0
80-81	32.495254609090665	36.0	31.5	37.0	20.0	37.0
82-83	32.38621889214991	36.0	31.0	37.0	20.0	37.0
84-85	32.28253509543627	36.0	31.0	37.0	19.5	37.0
86-87	32.14321749644245	36.0	31.0	37.0	19.0	37.0
88-89	32.02518876280929	36.0	30.0	37.0	18.0	37.0
90-91	31.92699167729817	36.0	30.0	37.0	18.0	37.0
92-93	31.864455620305364	36.0	30.0	37.0	18.0	37.0
94-95	31.762224677386676	36.0	30.0	37.0	17.5	37.0
96-97	31.74895977188012	36.0	30.0	37.0	17.5	37.0
98-99	32.094466718493145	36.0	30.5	37.0	19.5	37.0
100	34.11863753053927	36.0	33.0	37.0	27.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	179.0
20	12031.0
21	119016.0
22	385676.0
23	656244.0
24	812035.0
25	927990.0
26	1062548.0
27	1227767.0
28	1433526.0
29	1693121.0
30	2022043.0
31	2454863.0
32	3042736.0
33	3943557.0
34	5562970.0
35	9500996.0
36	1.5515679E7
37	507947.0
>>END_MODULE
>>Per base sequence content	pass
#Base	G	A	T	C
1	31.160783743126057	27.82196327250673	17.887219867421685	23.13003311694553
2	24.569915971903452	27.27222967751838	31.6724523090886	16.485402041489568
3	18.914119202641885	25.62357561883863	31.940391930195446	23.521913248324044
4	20.612633769720908	28.801811954109947	27.51451836135811	23.071035914811038
5	21.841378952823057	27.920443995075743	27.47322249209813	22.764954560003066
6	22.151652309771208	27.90587125470825	26.871706496805363	23.070769938715177
7	23.103926457045425	27.5965951435644	26.849715315114913	22.449763084275265
8	21.790710519558726	26.695470516591207	27.81375960163824	23.70005936221183
9	21.707928122592286	25.282214278494475	29.015862459279003	23.99399513963424
10-11	23.100555434929422	26.322865576777204	26.89615507421682	23.680423914076552
12-13	23.16923787567664	26.370467422452247	26.70233682556581	23.757957876305305
14-15	22.93874987851207	26.6452828246375	26.887649093403855	23.528318203446574
16-17	23.218129289528175	26.651029855423314	26.723280594282063	23.407560260766445
18-19	23.194008876407292	26.42512795619917	26.744954294355	23.635908873038538
20-21	23.12310241590792	26.537405398790316	26.85997337638067	23.4795188089211
22-23	23.298557544544522	26.764771874424113	26.632231260267535	23.304439320763827
24-25	23.226352414569686	26.571021827838347	26.746477067520335	23.456148690071636
26-27	23.12336673059873	26.5598080652108	26.92201548239496	23.39480972179551
28-29	23.36567589370216	26.618623533523678	26.645481342113147	23.370219230661018
30-31	23.359284590566023	26.436938568806145	26.63666713640071	23.567109704227125
32-33	23.24255840709245	26.515724671913205	26.75319759999547	23.488519320998876
34-35	23.442125164415206	26.642963964920575	26.54530586140387	23.36960500926035
36-37	23.433633071127876	26.43894437544186	26.618743806107464	23.508678747322797
38-39	23.298180939110356	26.499457867036742	26.744347884825764	23.45801330902714
40-41	23.52968975229512	26.569499712461248	26.482589237682554	23.41822129756108
42-43	23.503750940775898	26.395442111230118	26.52490368641639	23.575903261577594
44-45	23.3602566411735	26.438249234927998	26.700959646639838	23.50053447725867
46-47	23.568095730736985	26.521168694875858	26.51813099811124	23.392604576275914
48-49	23.54703932312673	26.36628622138349	26.55671785600507	23.52995659948471
50-51	23.429645524691235	26.398518753828338	26.672851630841183	23.498984090639244
52-53	23.665906432587043	26.46413967627341	26.431879856435252	23.438074034704297
54-55	23.638645825089426	26.32340754773928	26.458627430835985	23.579319196335305
56-57	23.49831658128196	26.35173315281672	26.661572543046525	23.488377722854796
58-59	23.70607973173159	26.424918321255415	26.466908015316232	23.40209393169676
60-61	23.71412779323042	26.258423839392098	26.49078137139137	23.536666995986106
62-63	23.565790696683855	26.288125793668343	26.636751010928457	23.509332498719345
64-65	23.79265698138811	26.394721186681906	26.39371766905284	23.418904162877144
66-67	23.766107270498313	26.22563138061869	26.452061364851136	23.55619998403186
68-69	23.617117993155897	26.275721097527192	26.618425476454643	23.488735432862264
70-71	23.848865693974584	26.349475160718654	26.40436992521011	23.397289220096653
72-73	23.87152362265966	26.187822562621733	26.398094159757036	23.54255965496158
74-75	23.762966231838067	26.205285212238667	26.518163585843702	23.513584970079563
76-77	23.94555459500518	26.31772236869226	26.326298613806514	23.41042442249605
78-79	23.900895839353165	26.16006617927394	26.401959073224756	23.537078908148143
80-81	23.81757867251649	26.18069227630973	26.51347417436479	23.488254876808988
82-83	24.03059269528062	26.283144472842118	26.273183501702384	23.413079330174874
84-85	24.030603147516715	26.117685611237274	26.30240781196856	23.54930342927745
86-87	23.945195736058846	26.126929884348904	26.416787105428696	23.511087274163557
88-89	24.187245455497326	26.19771603685055	26.158956205933233	23.456082301718894
90-91	24.18153487780602	26.02270362986745	26.21640456665372	23.579356925672815
92-93	24.057558963402464	26.087206369314686	26.345866470376468	23.509368196906383
94-95	24.271930660120052	26.16345703808245	26.11586366227793	23.448748639519565
96-97	24.241246624126077	26.033019222668592	26.145433679347907	23.58030047385742
98-99	24.045225354934292	26.043671841551262	26.32481697671721	23.586285826797237
100	24.641367937437334	25.415349710818376	25.91531924481945	24.027963106924844
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	19.0
1	12.0
2	6.0
3	6.5
4	8.0
5	12.5
6	22.0
7	42.0
8	92.0
9	168.0
10	323.0
11	626.0
12	1098.0
13	1894.0
14	3134.0
15	5162.0
16	8157.0
17	12256.0
18	18616.5
19	28115.5
20	42005.5
21	61090.5
22	85144.5
23	115261.5
24	152130.5
25	194611.5
26	243372.5
27	299303.5
28	361009.0
29	425198.5
30	493173.5
31	563688.5
32	634723.5
33	709859.0
34	785645.5
35	862402.0
36	945299.0
37	1033816.5
38	1125044.0
39	1221982.5
40	1325244.5
41	1434033.0
42	1545757.5
43	1659083.0
44	1770851.5
45	1877727.5
46	1980220.5
47	2074701.5
48	2153651.5
49	2208560.0
50	2236904.0
51	2229793.5
52	2181932.5
53	2096231.0
54	1976010.5
55	1825981.5
56	1651995.5
57	1461911.0
58	1268455.0
59	1084654.5
60	913436.0
61	757993.0
62	619518.5
63	499679.5
64	398408.0
65	313212.5
66	244732.5
67	188759.0
68	144150.5
69	109638.5
70	82230.5
71	60921.5
72	44919.0
73	32439.5
74	22910.5
75	16243.5
76	11312.0
77	7750.5
78	5304.5
79	3567.5
80	2376.5
81	1568.0
82	1020.5
83	673.5
84	459.5
85	306.0
86	196.5
87	118.5
88	75.5
89	51.0
90	35.0
91	31.0
92	22.0
93	13.5
94	14.5
95	12.5
96	13.0
97	11.5
98	6.5
99	4.5
100	2.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.07185600638856322
2	0.07150616997442892
3	0.11308167280924379
4	0.05585983462092788
5	0.02655808687751032
6	0.2802366560795948
7	0.05509137373370027
8	0.020003567545274926
9	0.056571299687875165
10-11	0.0282807364111548
12-13	0.08451988804291369
14-15	0.05327438629062633
16-17	0.04173764611664678
18-19	0.0615102823211308
20-21	0.028373108947471155
22-23	0.09028924081646002
24-25	0.08036803734146022
26-27	0.030918267128953868
28-29	0.0731855812995849
30-31	0.05091397318177633
32-33	0.01772766548028884
34-35	0.022832722141602618
36-37	0.01815808219206082
38-39	0.08691960861402595
40-41	0.042632873569670236
42-43	0.0170722135470653
44-45	0.11931288826437193
46-47	0.0716987765395141
48-49	0.003999534285187117
50-51	0.020133282170740452
52-53	0.006667528286239456
54-55	0.044513735638920394
56-57	0.019438522775254635
58-59	0.050289967218362625
60-61	0.06611023809237426
62-63	0.07989831552287598
64-65	0.01938938997172221
66-67	0.05829002477618402
68-69	0.018708390156784622
70-71	0.024884583164954007
72-73	0.021094362060540838
74-75	0.11419415703189292
76-77	0.02677037532209674
78-79	0.03140867432664768
80-81	0.02904925182183305
82-83	0.03393913965514249
84-85	0.03821489581812414
86-87	0.04311775963591815
88-89	0.044904028571595915
90-91	0.05520755622567158
92-93	0.054886594637074765
94-95	0.0634873897964209
96-97	0.044034194588661084
98-99	0.05613073804201399
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
61	1.0
62	1.0
63	1.0
64	2.0
65	0.0
66	1.0
67	1.0
68	5.0
69	10.0
70	3.0
71	4.0
72	2.0
73	2.0
74	8.0
75	7.0
76	11.0
77	11.0
78	11.0
79	11.0
80	7.0
81	29.0
82	26.0
83	55.0
84	64.0
85	119.0
86	211.0
87	364.0
88	670.0
89	1139.0
90	2109.0
91	4277.0
92	8300.0
93	17172.0
94	36172.0
95	80166.0
96	186336.0
97	455234.0
98	1266609.0
99	4805491.0
100	4.4016282E7
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	72.69329425308955
#Duplication Level	Percentage of total
1	59.89049304922437
2	14.060207748374284
3	8.505057044099825
4	5.038766625707894
5	3.1713737525488748
6	2.0307135244878936
7	1.4954087263246925
8	1.0867204157412205
9	0.8120680902344101
>10	3.7514475822450226
>50	0.1151880065275712
>100	0.03914365265286663
>500	0.003411781831072301
>1k	0.0
>5k	0.0
>10k+	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	1.9653731131140623E-6	1.9653731131140623E-6	5.896119339342186E-6	9.826865565570311E-6	2.751522358359687E-5	1.4150686414421247E-4
2	7.86149245245625E-6	1.9653731131140623E-6	7.86149245245625E-6	1.57229849049125E-5	7.861492452456249E-5	1.8081432640649372E-4
3	1.1792238678684372E-5	5.896119339342186E-6	1.1792238678684372E-5	2.1619104244254685E-5	9.040716320324686E-5	1.9653731131140622E-4
4	1.1792238678684372E-5	5.896119339342186E-6	1.3757611791798436E-5	2.948059669671093E-5	1.2381850612618592E-4	2.1029492310320466E-4
5	1.57229849049125E-5	9.826865565570311E-6	1.768835801802656E-5	3.9307462262281244E-5	1.5133372970978277E-4	2.181564155556609E-4
6	1.768835801802656E-5	1.3757611791798436E-5	1.768835801802656E-5	4.716895471473749E-5	1.8277969951960779E-4	2.2601790800811714E-4
7	1.9653731131140622E-5	1.3757611791798436E-5	2.1619104244254685E-5	5.896119339342186E-5	2.1029492310320466E-4	2.338794004605734E-4
8	2.1619104244254685E-5	1.3757611791798436E-5	2.1619104244254685E-5	6.485731273276405E-5	2.2601790800811714E-4	2.4174089291302965E-4
9	2.3584477357368745E-5	1.3757611791798436E-5	2.3584477357368745E-5	7.861492452456249E-5	2.3781014668680151E-4	2.594292509310562E-4
10-11	2.84979101401539E-5	1.57229849049125E-5	3.0463283253267964E-5	1.1104358089094452E-4	2.7122148960974054E-4	2.682734299400695E-4
12-13	3.1445969809825E-5	1.768835801802656E-5	3.635940259261015E-5	1.3462805824831326E-4	3.1445969809824995E-4	2.8203104173186793E-4
14-15	3.341134292293906E-5	2.1619104244254685E-5	4.520358160162343E-5	1.6116059527535308E-4	3.5474984691708823E-4	2.9578865352366634E-4
16-17	3.635940259261015E-5	2.1619104244254685E-5	5.011701438440859E-5	1.7197014739748042E-4	4.038841747449398E-4	3.056155190892367E-4
18-19	3.7342089149167184E-5	2.1619104244254685E-5	5.404776061063671E-5	1.8376238607616482E-4	4.569492487990195E-4	3.282173098900484E-4
20-21	4.225552193195234E-5	2.751522358359687E-5	5.9943879949978895E-5	1.89658505415507E-4	5.011701438440858E-4	3.409922351252898E-4
22-23	4.6186268158180456E-5	2.948059669671093E-5	6.97707455155492E-5	1.994853709810773E-4	5.483390985588233E-4	3.5966327969987337E-4
24-25	4.9134327827851556E-5	3.0463283253267964E-5	8.254567075079061E-5	2.1520835588598983E-4	6.00421486056346E-4	3.704728318220007E-4
26-27	5.3065074054079676E-5	3.537671603605312E-5	9.138984975980389E-5	2.368274601302445E-4	6.564346197800968E-4	3.881611898400273E-4
28-29	5.797850683686483E-5	4.029014881883827E-5	9.728596909914608E-5	2.633599971572843E-4	7.350495443046593E-4	4.0781492097116787E-4
30-31	6.387462617620703E-5	4.323820848850937E-5	9.925134221226015E-5	3.105289518720218E-4	8.028549167070943E-4	4.4122626389410695E-4
32-33	6.485731273276405E-5	4.323820848850937E-5	1.0907820777783045E-4	3.409922351252898E-4	9.15863870711153E-4	4.73654920260489E-4
34-35	7.370149174177733E-5	4.815164127129452E-5	1.1988775989995779E-4	3.4688835446463197E-4	0.0010013576011316146	4.962567110613007E-4
36-37	7.861492452456249E-5	4.9134327827851556E-5	1.3757611791798434E-4	3.626113393695445E-4	0.0010956955105610898	5.286853674276828E-4
38-39	8.156298419423357E-5	4.9134327827851556E-5	1.4642029692699764E-4	3.861958167269132E-4	0.0011752931216422093	5.738889490293061E-4
40-41	8.352835730734764E-5	5.011701438440859E-5	1.5035104315322574E-4	3.970053688490406E-4	0.0012539080461667716	6.230232768571578E-4
42-43	8.942447664668984E-5	5.404776061063671E-5	1.641086549450242E-4	4.10762980640839E-4	0.001359055507718374	6.672441719022241E-4
44-45	9.826865565570311E-5	5.503044716719374E-5	1.7590089362370855E-4	4.3139939832853667E-4	0.001485822073514231	7.212919325128608E-4
46-47	1.1104358089094452E-4	5.6013133723750776E-5	1.89658505415507E-4	4.5301850257279134E-4	0.0015998137140748466	7.753396931234975E-4
48-49	1.1595701367372966E-4	5.797850683686483E-5	1.9751999786796326E-4	4.726722337039319E-4	0.0017334590857666027	8.126817822726647E-4
50-51	1.2480119268274293E-4	6.190925306309296E-5	2.0636417687697653E-4	4.844644723826163E-4	0.001838606547318205	8.598507369874022E-4
52-53	1.3364537169175623E-4	6.485731273276405E-5	2.191391021122179E-4	5.011701438440858E-4	0.0019486674416525926	9.148811841545959E-4
54-55	1.4150686414421247E-4	6.780537240243515E-5	2.3191402734745934E-4	5.168931287489983E-4	0.0020685552015525504	9.7973849688736E-4
56-57	1.4838567004011167E-4	7.468417829833437E-5	2.3977551979991558E-4	5.434256657760382E-4	0.00218451221522628	0.0010554053617422513
58-59	1.523164162663398E-4	8.058029763767655E-5	2.515677584786E-4	5.650447700202929E-4	0.0023161922138049225	0.0011438471518323843
60-61	1.5428178937945387E-4	8.156298419423357E-5	2.643426837138414E-4	5.886292473776616E-4	0.0024478722123835645	0.0012372023747053021
62-63	1.6214328183191014E-4	8.451104386390467E-5	2.712214896097406E-4	6.01404172612903E-4	0.0025923271361974476	0.0013236787916823208
64-65	1.6509134150158121E-4	8.745910353357577E-5	2.771176089490828E-4	6.141790978481444E-4	0.0027652799701514854	0.0014288262532339232
66-67	1.680394011712523E-4	9.040716320324686E-5	2.8694447451465307E-4	6.299020827530569E-4	0.002941180863775194	0.001586056102283048
68-69	1.7491820706715155E-4	9.433790942947498E-5	2.997193997498945E-4	6.485731273276406E-4	0.003150493100321842	0.0017481993841149584
70-71	1.7688358018026559E-4	9.728596909914608E-5	3.0954626531546476E-4	6.760883509112374E-4	0.0033676668293209453	0.001900515800381298
72-73	1.7884895329337965E-4	1.012167153253742E-4	3.242865636638203E-4	6.967247685989351E-4	0.0036192345877995454	0.002051849530091081
74-75	1.8769313230239293E-4	1.0318208843848827E-4	3.3411342922939056E-4	7.242399921825319E-4	0.0039042136892010844	0.00221104475225332
76-77	1.9358925164173512E-4	1.1006089433438748E-4	3.498364141343031E-4	7.517552157661287E-4	0.004147919955227228	0.0023859629593204713
78-79	1.994853709810773E-4	1.1595701367372967E-4	3.606459662564304E-4	7.655128275579272E-4	0.00438573010191403	0.0025589157932745087
80-81	2.0341611720730543E-4	1.2185313301307186E-4	3.6654208559577263E-4	7.733743200103835E-4	0.004653020845297543	0.002746608925576902
82-83	2.1324298277287576E-4	1.228358195696289E-4	3.79317010831014E-4	7.92045364584967E-4	0.004921294275237612	0.0029559211621235496
84-85	2.2208716178188904E-4	1.3069731202208513E-4	3.891438763965843E-4	8.067856629333226E-4	0.00523968471956209	0.0031760429507923247
86-87	2.2601790800811714E-4	1.356107448048703E-4	3.989707419621546E-4	8.21525961281678E-4	0.005560040536999681	0.0033971474260176566
88-89	2.309313407909023E-4	1.4150686414421247E-4	4.0879760752772493E-4	8.372489461865905E-4	0.005850915757740563	0.003627096080252002
>>END_MODULE
