##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	SRR15674131
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	72394502
Total Bases	7.2 Gbp
Sequences flagged as poor quality	0
Sequence length	62-100
%GC	52
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	35.35561449127725	37.0	36.0	37.0	31.0	37.0
2	35.0068327702565	37.0	35.0	37.0	30.0	37.0
3	34.9525665913138	37.0	35.0	37.0	30.0	37.0
4	35.05641011246959	37.0	35.0	37.0	30.0	37.0
5	35.03553097167517	37.0	35.0	37.0	30.0	37.0
6	34.89944783375953	37.0	35.0	37.0	30.0	37.0
7	35.01399274768131	37.0	35.0	37.0	30.0	37.0
8	34.98974772973782	37.0	35.0	37.0	30.0	37.0
9	34.939200949265455	37.0	35.0	37.0	30.0	37.0
10-11	34.90081994762531	37.0	35.0	37.0	29.0	37.0
12-13	34.820410802743005	37.0	35.0	37.0	29.0	37.0
14-15	34.85725214326359	37.0	35.0	37.0	29.0	37.0
16-17	34.76789182139826	37.0	35.0	37.0	29.0	37.0
18-19	34.690340856271106	37.0	35.0	37.0	29.0	37.0
20-21	34.64968368039882	37.0	35.0	37.0	29.0	37.0
22-23	34.568596852838354	37.0	35.0	37.0	28.0	37.0
24-25	34.547111761332374	37.0	35.0	37.0	28.0	37.0
26-27	34.48824466670135	37.0	35.0	37.0	28.0	37.0
28-29	34.445341201463066	37.0	35.0	37.0	28.0	37.0
30-31	34.347764661741856	37.0	35.0	37.0	27.0	37.0
32-33	34.319441392110136	37.0	34.5	37.0	27.0	37.0
34-35	34.23949900919271	37.0	34.0	37.0	27.0	37.0
36-37	34.18524377030731	37.0	34.0	37.0	27.0	37.0
38-39	34.17702621947727	37.0	34.0	37.0	27.0	37.0
40-41	34.05742542437822	37.0	34.0	37.0	26.0	37.0
42-43	33.996814730488786	37.0	34.0	37.0	26.0	37.0
44-45	33.918145413860294	37.0	34.0	37.0	26.0	37.0
46-47	33.819485815373106	37.0	34.0	37.0	25.5	37.0
48-49	33.81837894955062	37.0	34.0	37.0	25.0	37.0
50-51	33.73258629502003	37.0	34.0	37.0	25.0	37.0
52-53	33.6014505010339	37.0	33.0	37.0	24.5	37.0
54-55	33.49886838782315	37.0	33.0	37.0	24.0	37.0
56-57	33.44570788676742	37.0	33.0	37.0	24.0	37.0
58-59	33.3530219670549	37.0	33.0	37.0	23.5	37.0
60-61	33.2491580299841	37.0	33.0	37.0	23.0	37.0
62-63	33.14160674178413	37.0	33.0	37.0	23.0	37.0
64-65	33.11251555017637	37.0	33.0	37.0	23.0	37.0
66-67	33.02319310284541	37.0	32.0	37.0	22.0	37.0
68-69	32.97286351310511	37.0	32.0	37.0	22.0	37.0
70-71	32.83509572565131	36.5	32.0	37.0	21.5	37.0
72-73	32.69516868790095	36.0	32.0	37.0	21.0	37.0
74-75	32.590570749133015	36.0	32.0	37.0	21.0	37.0
76-77	32.50870521940027	36.0	31.0	37.0	20.0	37.0
78-79	32.48901310683212	36.0	31.0	37.0	20.0	37.0
80-81	32.33171053119061	36.0	31.0	37.0	19.5	37.0
82-83	32.22466188313848	36.0	31.0	37.0	19.0	37.0
84-85	32.11944376728301	36.0	31.0	37.0	18.5	37.0
86-87	31.994954415421326	36.0	30.5	37.0	18.0	37.0
88-89	31.838433218291726	36.0	30.0	37.0	18.0	37.0
90-91	31.759524353929795	36.0	30.0	37.0	17.0	37.0
92-93	31.694258076975032	36.0	30.0	37.0	17.0	37.0
94-95	31.5845051203299	36.0	29.0	37.0	17.0	37.0
96-97	31.541520735977173	36.0	29.0	37.0	16.5	37.0
98-99	31.969497945647966	36.0	30.0	37.0	18.5	37.0
100	34.04873030490926	36.0	32.0	37.0	26.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	241.0
20	16333.0
21	161596.0
22	526487.0
23	909769.0
24	1144575.0
25	1325462.0
26	1538270.0
27	1794107.0
28	2102786.0
29	2487681.0
30	2960084.0
31	3564362.0
32	4367166.0
33	5565479.0
34	7785276.0
35	1.3664516E7
36	2.176114E7
37	719172.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	31.238750073429557	22.091928968565238	24.952596641936744	21.71672431606846
2	31.323382152451295	27.87372135251115	20.552826495273827	20.250069999763728
3	19.648620262998083	24.711235456765287	26.343131604726082	29.297012675510548
4	23.4307981372874	26.847713895570376	25.807417198242927	23.914070768899297
5	24.614332105135823	23.9642398483592	26.002497917837637	25.41893012866735
6	24.594979743776488	25.221748343596417	24.166465636365775	26.016806276261324
7	25.47429891575792	25.488162893363768	24.656429246211363	24.381108944666952
8	24.553249910985777	24.014329793768546	25.166003354462013	26.266416940783664
9	24.857956930931746	23.508753204619047	25.354065319265334	26.279224545183872
10-11	25.757194992149746	23.847686129469583	24.36148334923661	26.033635529144057
12-13	25.862551026346146	24.034098414731883	24.01484594871098	26.08850461021099
14-15	25.933860224827228	23.972221881615035	23.89684979033085	26.19706810322689
16-17	25.888174331788388	24.041170265982874	24.170617724551676	25.900037677677055
18-19	25.91696325182532	24.078088497449393	24.06421625383206	25.940731996893224
20-21	26.103754702401133	23.790308172179927	23.96597165256246	26.139965472856474
22-23	26.02963151432882	24.035987922467854	24.04833567710025	25.886044886103075
24-25	25.956964195192395	24.094354420261123	24.05251542679874	25.89616595774774
26-27	26.075913767754738	23.85072147476406	23.95870157228931	26.114663185191887
28-29	26.043582267136983	23.944435104888527	24.098327458996074	25.913655168978412
30-31	26.07315323650622	23.99749749069656	23.955043467338083	25.974305805459135
32-33	26.192156247891347	23.783265332936683	23.846671140861993	26.177907278309974
34-35	26.1340185304767	23.874231473257282	24.027578629329664	25.964171366936355
36-37	26.129093110371283	23.9674339364432	23.925209980528532	25.978262972656985
38-39	26.22187664950148	23.74678640555143	23.857369185005	26.17396775994209
40-41	26.217560727176753	23.81250200581772	23.941227474476946	26.02870979252858
42-43	26.20441381684042	23.890680174074845	23.85507337843251	26.049832630652226
44-45	26.32366502702451	23.625439870898422	23.81206976471129	26.23882533736578
46-47	26.297628172320742	23.764173098457935	23.8989995438773	26.039199185344025
48-49	26.23842077555682	23.84637592979019	23.87026944692752	26.044933847725467
50-51	26.398340622786886	23.60729373133152	23.745163230102097	26.2492024157795
52-53	26.36186999627857	23.70417787730032	23.856917278353333	26.077034848067775
54-55	26.3905287870352	23.73974599362537	23.773008022150393	26.09671719718904
56-57	26.50364772174868	23.514589056062306	23.695896966122874	26.285866256066136
58-59	26.449642624829785	23.623283256617025	23.84953211553273	26.077542003020465
60-61	26.458020460469378	23.694645497788816	23.747755745275853	26.099578296465953
62-63	26.547650790995387	23.472661084867124	23.70117210821162	26.278516015925874
64-65	26.52638315342025	23.546172054918195	23.813123402623805	26.11432138903774
66-67	26.5370195412097	23.631575985993365	23.69890734583546	26.132497126961475
68-69	26.62083661708163	23.439101683208	23.653415671454546	26.286646028255824
70-71	26.61042395187227	23.510502748907562	23.764543716360134	26.114529582860037
72-73	26.60680144386829	23.616108034127116	23.661936731229773	26.11515379077482
74-75	26.747522742681284	23.350875905218025	23.57403369163565	26.327567660465046
76-77	26.727569637007626	23.467566354486976	23.688221613599133	26.116642394906265
78-79	26.684620782783323	23.556352171477034	23.627081010108178	26.131946035631465
80-81	26.828275205771014	23.29420224709405	23.544607417800954	26.332915129333983
82-83	26.811105899836356	23.421551664053013	23.644996179372853	26.122346256737778
84-85	26.82498264022859	23.47775513176322	23.57288539366339	26.124376834344805
86-87	26.973997747738565	23.24470009192433	23.43155542881253	26.349746731524576
88-89	26.966730710247646	23.33574176654635	23.533820763340117	26.16370675986589
90-91	26.98035747225425	23.38544523409785	23.445624582611885	26.188572711036013
92-93	27.073503664038114	23.19081070736397	23.35376707607296	26.38191855252495
94-95	27.058782725041425	23.26584695158064	23.49048713848221	26.184883184895728
96-97	27.08518164243707	23.38379737182305	23.365349642235614	26.165671343504265
98-99	27.07850024158364	23.10035600547119	23.369831943593923	26.451311809351246
100	27.407249259569312	22.4910952037224	23.261444670513878	26.840210866194415
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	20.0
1	18.0
2	21.5
3	31.0
4	31.0
5	42.0
6	50.0
7	47.0
8	53.0
9	55.5
10	67.0
11	94.0
12	136.0
13	218.5
14	457.0
15	1023.5
16	1830.0
17	2696.0
18	3951.5
19	6101.5
20	9730.0
21	14508.5
22	21568.5
23	32393.0
24	46250.5
25	61109.0
26	80511.5
27	105952.0
28	136542.5
29	174117.0
30	215382.5
31	259347.5
32	309694.5
33	362716.5
34	417169.0
35	481034.5
36	551015.0
37	622486.5
38	707228.0
39	804937.5
40	907695.5
41	1025130.5
42	1160279.0
43	1314477.5
44	1500135.5
45	1718739.0
46	1965964.0
47	2244307.0
48	2550143.5
49	2868063.5
50	3174884.0
51	3451025.0
52	3676256.5
53	3820511.0
54	3867783.0
55	3807393.5
56	3653675.5
57	3424194.0
58	3142637.0
59	2846632.5
60	2535781.0
61	2213644.0
62	1902188.5
63	1617322.0
64	1362244.0
65	1132036.0
66	928208.0
67	758540.5
68	613711.0
69	486701.5
70	379243.5
71	288775.0
72	216124.5
73	159097.0
74	114025.0
75	80612.5
76	56754.5
77	38783.5
78	26198.0
79	17830.5
80	11659.0
81	7605.5
82	4973.5
83	3098.5
84	1974.0
85	1278.0
86	798.0
87	508.0
88	300.5
89	181.0
90	128.0
91	80.0
92	55.5
93	44.5
94	34.0
95	24.0
96	18.5
97	12.5
98	8.0
99	4.5
100	3.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.06388882956885317
2	0.08580071453492422
3	0.09167823269231136
4	0.05053836823133336
5	0.022958925803509223
6	0.2587364990783416
7	0.027474462080007124
8	0.060370606596616964
9	0.06381561958945446
10-11	0.06383426741439564
12-13	0.10207474042711144
14-15	0.02153409384596637
16-17	0.022241329873365245
18-19	0.0770873456661115
20-21	0.11103329366089153
22-23	0.08496915967458413
24-25	0.043352049027148495
26-27	0.053285123779151074
28-29	0.06068002235860398
30-31	0.07281354045366595
32-33	0.00940886367310048
34-35	0.07198129493314284
36-37	0.01577675056042239
38-39	0.009808755919061368
40-41	0.0586563880223943
42-43	0.020802684712162258
44-45	0.10789907775040707
46-47	0.061403143570212
48-49	0.009207881559845525
50-51	0.01747024932915486
52-53	0.0727368771733522
54-55	0.08671100465612706
56-57	0.03729979384346065
58-59	0.05081739494526809
60-61	0.06605957452404328
62-63	0.10664138629368142
64-65	0.03756224601014059
66-67	0.0701600293607954
68-69	0.01676854159437468
70-71	0.035403947290449114
72-73	0.05708447619813134
74-75	0.10235314787901945
76-77	0.1246615088763931
78-79	0.022891272093351303
80-81	0.11873299479226056
82-83	0.10454344007266426
84-85	0.06134404294601004
86-87	0.07579524853813287
88-89	0.10620132716883125
90-91	0.04329749536104678
92-93	0.06528342250836608
94-95	0.05309537231530724
96-97	0.09700435508217216
98-99	0.051980881454729816
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
62	1.0
63	0.0
64	3.0
65	1.0
66	1.0
67	3.0
68	8.0
69	14.0
70	2.0
71	4.0
72	4.0
73	6.0
74	7.0
75	6.0
76	6.0
77	24.0
78	16.0
79	16.0
80	26.0
81	26.0
82	40.0
83	77.0
84	117.0
85	193.0
86	362.0
87	687.0
88	1261.0
89	2074.0
90	3916.0
91	7675.0
92	14666.0
93	28450.0
94	58147.0
95	126365.0
96	289536.0
97	688498.0
98	1910203.0
99	7182314.0
100	6.2079747E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	32.286880016951095
#Duplication Level	Percentage of total
1	15.366174661044706
2	11.123916227043352
3	10.405603716404729
4	8.892031714805638
5	7.285708701456474
6	5.877477132160151
7	4.94203431167576
8	4.0242488422533995
9	3.3658622771520736
>10	25.787174299810722
>50	1.9810889530000115
>100	0.8968335162599617
>500	0.03607874462214909
>1k	0.015766902310675873
>5k	0.0
>10k+	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	9.66924256209401E-6	0.0	4.1439610980402906E-6	1.3813203660134301E-6	3.177036841830889E-5	9.66924256209401E-5
2	1.5194524026147732E-5	0.0	8.287922196080581E-6	6.90660183006715E-6	3.2875424711119636E-4	1.243188329412087E-4
3	1.5194524026147732E-5	1.3813203660134301E-6	1.1050562928107441E-5	9.66924256209401E-6	4.475477985883514E-4	1.4227599769938332E-4
4	1.6575844392161162E-5	1.3813203660134301E-6	1.2431883294120872E-5	1.5194524026147732E-5	5.773919129936138E-4	1.519452402614773E-4
5	2.2101125856214882E-5	1.3813203660134301E-6	4.2820931346416336E-5	1.933848512418802E-5	7.749207253335344E-4	1.6161448282357133E-4
6	2.6245086954255174E-5	2.7626407320268602E-6	6.0778096104590926E-5	2.2101125856214882E-5	9.95931983895683E-4	1.7128372538566535E-4
7	2.6245086954255174E-5	5.5252814640537205E-6	6.492205720263122E-5	2.2101125856214882E-5	0.0012307564461179663	1.795716475817459E-4
8	2.9007727686282032E-5	1.1050562928107441E-5	6.630337756864465E-5	3.453300915033575E-5	0.0014393358213859943	1.933848512418802E-4
9	3.7295649882362613E-5	1.1050562928107441E-5	7.597262013073865E-5	5.93967757385775E-5	0.0016327206726278745	2.0443541416998766E-4
10-11	4.14396109804029E-5	1.2431883294120872E-5	8.840450342485953E-5	6.561271738563792E-5	0.0019110567263795806	2.1893927801312868E-4
12-13	4.834621281047005E-5	1.38132036601343E-5	9.738308580394683E-5	7.182865903269836E-5	0.0023130209528894885	2.368964427713033E-4
14-15	6.008743592158421E-5	1.7266504575167876E-5	1.056710080000274E-4	8.080724141178566E-5	0.002726726402510511	2.6797615100660545E-4
16-17	6.699403775165136E-5	2.1410465673208165E-5	1.1326827001310127E-4	9.18578043398931E-5	0.0030361421644975193	2.8455199539876663E-4
18-19	7.113799884969166E-5	2.2101125856214882E-5	1.18793551477155E-4	1.0774298854904755E-4	0.0034332717697263806	3.031998203399479E-4
20-21	7.459129976472523E-5	3.0389048052295463E-5	1.2570015330722213E-4	1.1326827001310127E-4	0.0037558100751905166	3.308262276602165E-4
22-23	8.011658122877895E-5	3.7295649882362613E-5	1.319160949542826E-4	1.2224685239218858E-4	0.0041190973314520485	3.5223669333342466E-4
24-25	8.149790159479238E-5	6.146875628759764E-5	1.415853375163766E-4	1.277721338562423E-4	0.0044374916758181445	3.798631006536933E-4
26-27	8.909516360786624E-5	6.561271738563794E-5	1.5677986154252432E-4	1.415853375163766E-4	0.004847743824524133	4.088708283399753E-4
28-29	9.66924256209401E-5	6.90660183006715E-5	1.7680900684971906E-4	1.650677837386049E-4	0.005244182769569988	4.406411967582842E-4
30-31	1.0014572653597369E-4	7.459129976472523E-5	1.878595697778265E-4	1.8509692904579965E-4	0.0056081606860145265	4.841527882877073E-4
32-33	1.049803478170207E-4	7.459129976472523E-5	2.0098211325495407E-4	2.0029145307194737E-4	0.005952800117334877	5.242110789020968E-4
34-35	1.105056292810744E-4	8.149790159479238E-5	2.106513558170481E-4	2.154859770980951E-4	0.0062856983255441135	5.663413500655064E-4
36-37	1.2017487184316842E-4	9.462044507191996E-5	2.2446455947718242E-4	2.244645594771824E-4	0.006593042106982102	6.098529415949293E-4
38-39	1.28462794039249E-4	1.0152704690198711E-4	2.4034974368633685E-4	2.382777631373167E-4	0.006978430489099849	6.56817834039386E-4
40-41	1.3675071623532958E-4	1.0221770708499383E-4	2.534722871634644E-4	2.479470056994107E-4	0.007363128211034589	7.162146097779635E-4
42-43	1.4227599769938332E-4	1.056710080000274E-4	2.6383218990856514E-4	2.541629473464712E-4	0.007819654592002028	7.997844919217761E-4
44-45	1.5125458007847058E-4	1.0843364873205427E-4	2.686668111896122E-4	2.7281077228765246E-4	0.008263749089675346	8.736851315034945E-4
46-47	1.5816118190853775E-4	1.1395893019610799E-4	2.804080343007263E-4	2.873146361307935E-4	0.008723728771557818	9.710682173074414E-4
48-49	1.6713976428762506E-4	1.194842116601617E-4	2.9007727686282034E-4	2.9422123796086065E-4	0.00913743422117884	0.0010643073420133478
50-51	1.7473702630069893E-4	1.2500949312421543E-4	3.121784027190352E-4	2.9629321850988075E-4	0.009528347884760641	0.0011692876898303686
52-53	1.7819032721573248E-4	1.277721338562423E-4	3.204663249151158E-4	3.0250916015694116E-4	0.01021624542703533	0.0012804839792944496
54-55	1.8509692904579965E-4	1.319160949542826E-4	3.308262276602165E-4	3.0803444162099487E-4	0.010879969862904782	0.0014110187538827188
56-57	1.9476617160789367E-4	1.3467873568630945E-4	3.4256745077133064E-4	3.114877425360285E-4	0.011402799621440866	0.0015229057035298066
58-59	2.065073947190078E-4	1.4089467733336986E-4	3.722658386406194E-4	3.239196258301494E-4	0.011848275439480197	0.0016810668854383443
60-61	2.1341399654907495E-4	1.4503863843141017E-4	3.888416830327806E-4	3.384234896732904E-4	0.012361435955454185	0.001868926455216171
62-63	2.1824861783012196E-4	1.4987325971245718E-4	3.9436696449683434E-4	3.432581109543374E-4	0.013034829633885733	0.002036756879686803
64-65	2.2722720020920928E-4	1.5332656062749074E-4	4.081801681569686E-4	3.5776197479747837E-4	0.01372756179744147	0.0022384296531247636
66-67	2.3551512240528984E-4	1.5677986154252434E-4	4.1923073108507603E-4	3.6466857662754553E-4	0.014334652098304371	0.0024663475135169796
68-69	2.479470056994107E-4	1.6437712355559818E-4	4.2337469218311634E-4	3.701938580915993E-4	0.014916187972396026	0.0027281077228765247
70-71	2.555442677124846E-4	1.7128372538566535E-4	4.406411967582842E-4	3.8884168303278063E-4	0.01573116698834395	0.003002990475713197
72-73	2.652135102745786E-4	1.7335570593468547E-4	4.5031043932037824E-4	3.9989224596088805E-4	0.016387294162200326	0.003301355674772098
74-75	2.721201121046457E-4	1.8095296794775934E-4	4.6619562352953267E-4	4.0748950797396186E-4	0.01692669976512857	0.003606627475661066
76-77	2.838613352157599E-4	1.8924089014383993E-4	4.813901475556804E-4	4.1854007090206933E-4	0.01760423740465816	0.003945741625517364
78-79	2.9629321850988075E-4	1.926941910588735E-4	4.876060892027408E-4	4.254466727321365E-4	0.018220996948083157	0.004266898610615485
80-81	3.0181849997393446E-4	1.9683815215691382E-4	5.028006132288885E-4	4.358065754772372E-4	0.01864022767916823	0.004608084741020803
82-83	3.114877425360285E-4	2.0927003545103467E-4	5.117791956079759E-4	4.420225171242976E-4	0.019138193671116074	0.005012811608262738
84-85	3.239196258301494E-4	2.1272333636606825E-4	5.255923992681102E-4	4.5721704115044535E-4	0.019536013936527943	0.005417538475504673
86-87	3.322075480262299E-4	2.1962993819613538E-4	5.311176807321639E-4	4.7448354572561324E-4	0.019837141776318873	0.0059120511665374815
88-89	3.4463943132035085E-4	2.210112585621488E-4	5.42858903843278E-4	4.8346212810470055E-4	0.020103045946776456	0.006360980285491846
>>END_MODULE
