##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	SRR15674140
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	29587860
Total Bases	2.9 Gbp
Sequences flagged as poor quality	0
Sequence length	65-100
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	35.38878519771285	37.0	36.0	37.0	31.0	37.0
2	35.03570471132417	37.0	36.0	37.0	30.0	37.0
3	35.171317560648184	37.0	36.0	37.0	30.0	37.0
4	35.25674202189682	37.0	36.0	37.0	31.0	37.0
5	35.226491743573206	37.0	36.0	37.0	31.0	37.0
6	35.1421965292522	37.0	36.0	37.0	30.0	37.0
7	35.2246698814987	37.0	36.0	37.0	31.0	37.0
8	35.193839669377915	37.0	36.0	37.0	30.0	37.0
9	35.20915091527404	37.0	36.0	37.0	30.0	37.0
10-11	35.13508587643716	37.0	36.0	37.0	30.0	37.0
12-13	35.16071975127637	37.0	36.0	37.0	30.0	37.0
14-15	35.16671805598648	37.0	36.0	37.0	30.0	37.0
16-17	35.11399095777796	37.0	36.0	37.0	30.0	37.0
18-19	35.087130971959446	37.0	36.0	37.0	30.0	37.0
20-21	35.083269607872964	37.0	36.0	37.0	30.0	37.0
22-23	35.02920944603632	37.0	36.0	37.0	30.0	37.0
24-25	35.02370343782889	37.0	36.0	37.0	30.0	37.0
26-27	34.98297217169474	37.0	35.5	37.0	30.0	37.0
28-29	35.026715331896256	37.0	36.0	37.0	30.0	37.0
30-31	34.928550459546585	37.0	35.0	37.0	29.5	37.0
32-33	34.87556619505432	37.0	35.0	37.0	29.0	37.0
34-35	34.85976023612387	37.0	35.0	37.0	29.0	37.0
36-37	34.83273350962185	37.0	35.0	37.0	29.0	37.0
38-39	34.77031253696617	37.0	35.0	37.0	29.0	37.0
40-41	34.75104382337891	37.0	35.0	37.0	29.0	37.0
42-43	34.724371482087584	37.0	35.0	37.0	29.0	37.0
44-45	34.68479359439986	37.0	35.0	37.0	29.0	37.0
46-47	34.66807433859698	37.0	35.0	37.0	29.0	37.0
48-49	34.6783454599285	37.0	35.0	37.0	29.0	37.0
50-51	34.61629558541915	37.0	35.0	37.0	28.5	37.0
52-53	34.50614177571477	37.0	35.0	37.0	28.0	37.0
54-55	34.46535323947052	37.0	35.0	37.0	28.0	37.0
56-57	34.454473338051486	37.0	35.0	37.0	28.0	37.0
58-59	34.38856221774741	37.0	35.0	37.0	27.5	37.0
60-61	34.30335801575376	37.0	35.0	37.0	27.0	37.0
62-63	34.248482773002166	37.0	35.0	37.0	27.0	37.0
64-65	34.28690773851167	37.0	35.0	37.0	27.0	37.0
66-67	34.24419233916181	37.0	35.0	37.0	27.0	37.0
68-69	34.16833078098668	37.0	34.0	37.0	27.0	37.0
70-71	34.1055180532057	37.0	34.0	37.0	26.5	37.0
72-73	34.04250637160071	37.0	34.0	37.0	26.0	37.0
74-75	33.962922589583975	37.0	34.0	37.0	26.0	37.0
76-77	33.99469427099375	37.0	34.0	37.0	26.0	37.0
78-79	33.92267415187063	37.0	34.0	37.0	26.0	37.0
80-81	33.85207632652535	37.0	34.0	37.0	26.0	37.0
82-83	33.817537950432154	37.0	34.0	37.0	25.0	37.0
84-85	33.73068804758827	37.0	34.0	37.0	25.0	37.0
86-87	33.659650909669686	37.0	34.0	37.0	25.0	37.0
88-89	33.585253690308136	37.0	33.5	37.0	24.5	37.0
90-91	33.52000918341535	37.0	33.0	37.0	24.0	37.0
92-93	33.4572575330249	37.0	33.0	37.0	24.0	37.0
94-95	33.414456435060046	37.0	33.0	37.0	24.0	37.0
96-97	33.389069180378364	37.0	33.0	37.0	24.0	37.0
98-99	33.545379943457505	37.0	33.0	37.0	24.5	37.0
100	34.61240555844636	37.0	34.0	37.0	28.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	54.0
20	4156.0
21	40965.0
22	127118.0
23	209434.0
24	258853.0
25	296804.0
26	346207.0
27	410808.0
28	495150.0
29	610138.0
30	757930.0
31	967956.0
32	1292256.0
33	1876762.0
34	3187723.0
35	6841529.0
36	1.1539676E7
37	324341.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	29.454580726625956	26.69927976706082	18.298645107055485	25.547494399257737
2	29.821702915993587	28.220084760769602	22.8569739181386	19.101238405098208
3	17.00302546621405	27.774151710760087	31.329935719618607	23.892887103407254
4	19.523611114584774	28.11544979575647	30.083259547086392	22.277679542572358
5	22.52089769791375	27.44699934211421	27.89573737756456	22.136365582407482
6	21.95078104145651	27.669809271096433	27.704924727964332	22.674484959482726
7	23.393117622805562	28.480884321480332	26.609208272126462	21.516789783587647
8	21.597252126614393	26.975751701316874	28.07614879783075	23.350847374237976
9	21.981694910671436	26.42612395817138	28.65576900978119	22.93641212137599
10-11	23.362697933391967	27.000554775245956	26.8541786010707	22.78256869029138
12-13	23.205710660031468	27.32118953671287	26.63851044532629	22.834589357929367
14-15	23.223645618259575	27.503724854568517	26.480537114931806	22.792092412240102
16-17	23.492654804535007	27.434701139348988	26.366290163109905	22.706353893006103
18-19	23.220068194469903	27.377052784088953	26.515304524630707	22.887574496810444
20-21	23.216130345479904	27.40474897602508	26.53815000525046	22.84097067324456
22-23	23.436935533089898	27.44488840421778	26.379194334119017	22.7389817285733
24-25	23.230831563437516	27.40316790019231	26.56155128636718	22.804449250002996
26-27	23.23605480805808	27.388377173164013	26.584429181074125	22.791138837703784
28-29	23.484929386846293	27.314969332764733	26.388071225534965	22.812030054854013
30-31	23.257242277497966	27.279852154505484	26.543329147922723	22.919576420073827
32-33	23.225748416780174	27.352504411254092	26.53104186250752	22.890705309458216
34-35	23.45630586539912	27.34266615344912	26.39626015586245	22.804767825289314
36-37	23.296787728945308	27.297148896003627	26.533204183832748	22.87285919121832
38-39	23.282319262580476	27.321063167671923	26.52116451024612	22.87545305950148
40-41	23.531409951367902	27.27133373500142	26.319927985217404	22.877328328413277
42-43	23.312669964312988	27.269319826299498	26.465073952267932	22.952936257119585
44-45	23.2544103497262	27.3632532595881	26.484892094286234	22.89744429639947
46-47	23.495646330905355	27.327159450195733	26.3652281460132	22.81196607288571
48-49	23.316815913079157	27.2851206925587	26.4951266438463	22.902936750515845
50-51	23.322415308690907	27.286738400261477	26.467889087237783	22.92295720380983
52-53	23.533968421594736	27.249907042000782	26.31436860483003	22.901755931574456
54-55	23.32717167183443	27.245246664494633	26.481149205447835	22.9464324582231
56-57	23.307053983217905	27.2998834427147	26.491869107177685	22.901193466889705
58-59	23.552612042897145	27.24791975776081	26.333494208330983	22.865973991011067
60-61	23.375509132957472	27.197468812909037	26.455096197320245	22.971925856813243
62-63	23.348715107256286	27.20925369035484	26.458257117450763	22.983774084938112
64-65	23.586411971059903	27.19045816567504	26.315412253252095	22.90771761001296
66-67	23.362122733552443	27.200238984017737	26.47315235337615	22.964485929053666
68-69	23.349184188031817	27.27381622143995	26.446429945900217	22.93056964462801
70-71	23.61723574448227	27.199952089068898	26.285947629586232	22.896864536862605
72-73	23.419404415502235	27.1628121695678	26.422069274986328	22.995714139943633
74-75	23.406953822899414	27.20232803526137	26.407841476629606	22.982876665209613
76-77	23.599105919764714	27.199568217016612	26.279082053199264	22.922243810019406
78-79	23.411334165175234	27.171695999663743	26.438831663571253	22.97813817158977
80-81	23.409755193055204	27.191693221910484	26.43167593824536	22.966875646788953
82-83	23.657047124991625	27.163686225247503	26.247839631698238	22.931427018062635
84-85	23.469549763856463	27.11900050758269	26.38150177493331	23.029947953627538
86-87	23.464301094751104	27.154076431786116	26.372292446535134	23.009330026927646
88-89	23.673182261168378	27.131666601983785	26.22228501945828	22.972866117389557
90-91	23.526611860874297	27.07622300566011	26.358264939349862	23.038900194115726
92-93	23.501090287225036	27.117866851645147	26.35543253639308	23.02561032473674
94-95	23.729727831944228	27.064923499855	26.20221036723033	23.00313830097045
96-97	23.53874920138226	27.05244356904893	26.311615410268242	23.09719181930057
98-99	23.465118137647327	27.089134671741487	26.34598381106056	23.09976337955063
100	24.055418725017663	26.363914737567246	26.05275703630457	23.52790950111052
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	6.0
1	5.0
2	2.5
3	1.0
4	1.5
5	1.5
6	1.0
7	2.0
8	8.0
9	15.5
10	27.5
11	59.5
12	110.0
13	199.5
14	389.0
15	760.0
16	1456.0
17	2765.0
18	4806.5
19	7907.5
20	12244.5
21	18542.0
22	27475.5
23	38830.5
24	53549.0
25	72596.5
26	95852.0
27	122309.5
28	152689.5
29	187992.0
30	228058.5
31	274459.0
32	325762.5
33	382069.5
34	446949.0
35	519595.0
36	601242.0
37	692248.5
38	793006.5
39	901209.5
40	1005273.0
41	1103166.0
42	1199216.5
43	1287025.5
44	1357403.0
45	1405418.5
46	1428531.0
47	1428112.5
48	1399185.0
49	1341381.0
50	1266134.0
51	1173052.0
52	1068774.0
53	965290.0
54	859770.0
55	756443.5
56	664884.0
57	581922.5
58	508747.0
59	445767.5
60	390191.0
61	343289.5
62	299915.5
63	258556.5
64	221773.0
65	187990.0
66	156338.5
67	128543.0
68	103794.0
69	82442.0
70	63997.0
71	48203.5
72	35775.0
73	25955.5
74	18296.0
75	12572.5
76	8453.0
77	5646.5
78	3686.0
79	2343.0
80	1465.0
81	876.0
82	539.0
83	333.0
84	185.0
85	109.0
86	70.0
87	38.0
88	23.5
89	16.0
90	13.5
91	7.0
92	3.0
93	1.5
94	1.0
95	1.0
96	0.5
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.10347825087721789
2	0.14234216330616678
3	0.02465538230882531
4	4.799265644761061E-4
5	0.05873354815116741
6	0.11465175244171089
7	0.026524392098651272
8	0.04882069875955882
9	0.0015546916877394984
10-11	0.15603358945189008
12-13	0.023087171562931552
14-15	0.05940950105888023
16-17	0.06688723010045336
18-19	0.0658378132112292
20-21	0.08037587037386279
22-23	0.11577890391532204
24-25	0.12001036911760432
26-27	0.10909541954031146
28-29	0.0223571424226017
30-31	0.08668082112055417
32-33	0.05808970300657094
34-35	0.02420587362519628
36-37	0.0015006154551224725
38-39	0.1370646609791989
40-41	0.07712284700549482
42-43	0.03241870145390711
44-45	0.0545510895346943
46-47	0.02736595346875374
48-49	0.014688456684599697
50-51	0.013203050169900764
52-53	0.08374211585427267
54-55	0.11813797956323978
56-57	0.02118605400998923
58-59	0.06891339894132256
60-61	0.05288824538172075
62-63	0.14145328523252443
64-65	0.005546193607783733
66-67	0.020158606271579164
68-69	0.061013206549788424
70-71	0.026913072752173302
72-73	0.03735317535450198
74-75	0.14565781816066084
76-77	0.005644212692996164
78-79	0.0516783647810178
80-81	0.05977463078282629
82-83	0.007597733664995138
84-85	0.04467395490685076
86-87	0.015626476811479512
88-89	0.024091385031570937
90-91	0.026126801105788973
92-93	0.03527798351296379
94-95	0.024340394910902927
96-97	0.044999404349900636
98-99	0.024043597182013612
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
65	1.0
66	0.0
67	2.0
68	1.0
69	4.0
70	1.0
71	4.0
72	2.0
73	5.0
74	3.0
75	4.0
76	8.0
77	5.0
78	6.0
79	5.0
80	12.0
81	15.0
82	23.0
83	26.0
84	47.0
85	48.0
86	69.0
87	117.0
88	224.0
89	415.0
90	672.0
91	1287.0
92	2494.0
93	5006.0
94	10228.0
95	22657.0
96	52601.0
97	131114.0
98	385811.0
99	1823759.0
100	2.7151184E7
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	77.80207798369105
#Duplication Level	Percentage of total
1	67.48879891008043
2	11.528527854307024
3	6.348090398521723
4	3.980547943978738
5	2.715777027679783
6	1.878306212455191
7	1.320163161148458
8	0.8953931222425028
9	0.6683823873809586
>10	2.8961041404638546
>50	0.21407246270826233
>100	0.06583637903298138
>500	0.0
>1k	0.0
>5k	0.0
>10k+	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	6.759529077128255E-6	3.3797645385641275E-6	3.3797645385641275E-6	1.6898822692820636E-5	6.759529077128255E-6	1.351905815425651E-5
2	6.759529077128255E-6	3.3797645385641275E-6	1.0139293615692382E-5	2.703811630851302E-5	4.393693900133365E-5	1.6898822692820636E-5
3	1.0139293615692382E-5	1.0139293615692382E-5	1.0139293615692382E-5	4.0557174462769526E-5	5.407623261702604E-5	2.365835176994889E-5
4	1.6898822692820636E-5	2.0278587231384763E-5	2.0278587231384763E-5	4.393693900133365E-5	6.759529077128255E-5	4.393693900133365E-5
5	2.0278587231384763E-5	2.703811630851302E-5	2.703811630851302E-5	5.7455997155590166E-5	6.759529077128255E-5	4.731670353989778E-5
6	3.379764538564127E-5	3.379764538564127E-5	2.703811630851302E-5	7.097505530984667E-5	7.773458438697493E-5	5.7455997155590166E-5
7	3.71774099242054E-5	3.379764538564127E-5	3.0417880847077146E-5	7.097505530984667E-5	8.111434892553905E-5	6.083576169415429E-5
8	4.0557174462769526E-5	4.0557174462769526E-5	3.379764538564127E-5	8.111434892553905E-5	8.449411346410318E-5	7.097505530984667E-5
9	4.0557174462769526E-5	4.731670353989778E-5	3.379764538564127E-5	8.449411346410318E-5	8.78738780026673E-5	8.449411346410318E-5
10-11	4.393693900133365E-5	4.731670353989778E-5	3.71774099242054E-5	9.80131716183597E-5	9.80131716183597E-5	9.970305388764175E-5
12-13	5.069646807846191E-5	5.7455997155590166E-5	4.731670353989778E-5	1.0815246523405208E-4	1.115322297726162E-4	1.2843105246543684E-4
14-15	7.266493757912874E-5	8.111434892553905E-5	4.731670353989778E-5	1.3688046381184714E-4	1.1998164111902652E-4	1.487096396968216E-4
16-17	8.956376027194937E-5	8.956376027194937E-5	5.069646807846191E-5	1.5039952196610365E-4	1.2843105246543684E-4	1.7405787373605256E-4
18-19	1.0308281842620588E-4	1.0139293615692383E-4	5.57661148863081E-5	1.6391858012036015E-4	1.3688046381184714E-4	2.0278587231384765E-4
20-21	1.115322297726162E-4	1.115322297726162E-4	6.421552623271842E-5	1.7236799146677048E-4	1.4532987515825747E-4	2.1461504819882208E-4
22-23	1.3350069927328304E-4	1.267411701961548E-4	6.759529077128255E-5	1.7912752054389874E-4	1.6053881558179605E-4	2.247543418145145E-4
24-25	1.5208940423538572E-4	1.3181081700400097E-4	7.604470211769286E-5	2.044757545831297E-4	1.6898822692820636E-4	2.3658351769948892E-4
26-27	1.5546916877394985E-4	1.537792865046678E-4	7.942446665625699E-5	2.3996328223805302E-4	1.8419716735174491E-4	2.602418694694378E-4
28-29	1.6898822692820636E-4	1.5884893331251398E-4	8.449411346410318E-5	2.551722226615916E-4	1.960263432367194E-4	2.788305744315405E-4
30-31	1.9771622550600145E-4	1.706781091974884E-4	8.449411346410318E-5	2.788305744315405E-4	2.1461504819882208E-4	3.1769786662502796E-4
32-33	2.0616563685241178E-4	1.9264657869815524E-4	8.956376027194937E-5	3.058686907400535E-4	2.2644422408379654E-4	3.498056297413872E-4
34-35	2.2982398862236066E-4	2.0616563685241178E-4	9.125364254123143E-5	3.210776311635921E-4	2.4334304677661717E-4	3.836032751270284E-4
36-37	2.534823403923095E-4	2.1968469500666828E-4	9.632328934907762E-5	3.345966893178486E-4	2.602418694694378E-4	4.3091997866692624E-4
38-39	2.670013985465661E-4	2.247543418145145E-4	9.970305388764175E-5	3.548752765492334E-4	2.9065975031651494E-4	5.57661148863081E-4
40-41	2.8221033897010467E-4	2.281341063530786E-4	1.0139293615692383E-4	3.7346398151133606E-4	3.278371602407203E-4	5.779397360944657E-4
42-43	2.9065975031651494E-4	2.7207104535441226E-4	1.0984234750333414E-4	3.836032751270284E-4	3.4642586520282305E-4	6.066677346722609E-4
44-45	2.991091616629253E-4	2.9065975031651494E-4	1.1829175884974446E-4	3.954324510120029E-4	3.650145701649258E-4	6.421552623271842E-4
46-47	3.126282198171818E-4	2.974192793936432E-4	1.351905815425651E-4	4.0219198008913115E-4	3.7853362831918224E-4	6.844023190592358E-4
48-49	3.2445739570215626E-4	3.227675134328742E-4	1.3857034608112922E-4	4.2247056732051596E-4	4.1571103824338766E-4	7.16510082175595E-4
50-51	3.3121692477928446E-4	3.2783716024072036E-4	1.4195011061969335E-4	4.359896254747724E-4	4.51198565898311E-4	7.452380807533901E-4
52-53	3.3290680704856654E-4	3.379764538564127E-4	1.487096396968216E-4	4.478188013597469E-4	4.8499621128395226E-4	7.824154906775954E-4
54-55	3.430461006642589E-4	3.582550410877975E-4	1.5039952196610365E-4	4.748569176682599E-4	5.204837389388756E-4	8.263524296789292E-4
56-57	3.481157474721051E-4	3.7515386378061814E-4	1.6391858012036015E-4	4.900658580917985E-4	5.644206779402093E-4	8.618399573338525E-4
58-59	3.531853942799513E-4	3.971223332812849E-4	1.6898822692820636E-4	5.086545630539011E-4	6.218766750957994E-4	9.125364254123143E-4
60-61	3.683943347034899E-4	4.055717446276953E-4	1.7743763827461666E-4	5.407623261702604E-4	6.776427899821075E-4	9.514037176058018E-4
62-63	3.802235105884643E-4	4.123312737048235E-4	1.8419716735174491E-4	5.492117375166708E-4	7.283392580605694E-4	0.0010257585374542125
64-65	3.852931573963105E-4	4.2923009639764416E-4	1.8926681415959112E-4	5.661105602094913E-4	7.857952552161596E-4	0.0010747651232633926
66-67	3.903628042041567E-4	4.7316703539897783E-4	1.8926681415959112E-4	5.863891474408761E-4	8.466310169103139E-4	0.0011271514736111365
68-69	4.0895150916625946E-4	4.934456226303625E-4	1.8926681415959112E-4	5.948385587872864E-4	9.361947771822632E-4	0.0011964366466517009
70-71	4.190908027819518E-4	5.187938566695936E-4	1.8926681415959112E-4	5.999082055951326E-4	0.00100716983249211	0.0012505128792687271
72-73	4.2923009639764416E-4	5.255533857467218E-4	1.8926681415959112E-4	6.066677346722608E-4	0.0010849044168790849	0.0013045891118857531
74-75	4.478188013597469E-4	5.289331502852859E-4	1.9433646096743732E-4	6.184969105572354E-4	0.0011744681771510344	0.0013907731076191383
76-77	4.630277417832855E-4	5.323129148238501E-4	2.0447575458312973E-4	6.438451445964662E-4	0.0012758611133079582	0.001487096396968216
78-79	4.816164467453881E-4	5.373825616316962E-4	2.1461504819882208E-4	6.675034963664152E-4	0.001351905815425651	0.0015715905104323191
80-81	4.917557403610806E-4	5.458319729781066E-4	2.1799481273738623E-4	6.793326722513896E-4	0.001461748162928985	0.0016662239175121148
82-83	4.968253871689267E-4	5.711802070173375E-4	2.247543418145145E-4	6.877820835978E-4	0.0016070780380872426	0.0017591674423226282
84-85	5.103444453231832E-4	5.84699265171594E-4	2.332037531609248E-4	6.996112594827744E-4	0.0017895853231697054	0.0018656300252873983
86-87	5.340027970931322E-4	6.049778524029787E-4	2.3658351769948892E-4	7.114404353677488E-4	0.0019163264933658602	0.0020025104890992454
88-89	5.424522084395424E-4	6.303260864422098E-4	2.450329290458992E-4	7.283392580605694E-4	0.0020379980167541684	0.002092074249371195
>>END_MODULE
