##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	SRR15674141
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	46131452
Total Bases	4.6 Gbp
Sequences flagged as poor quality	0
Sequence length	61-100
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	35.323696401318564	37.0	36.0	37.0	31.0	37.0
2	34.98366168920935	37.0	35.0	37.0	30.0	37.0
3	35.21660115966001	37.0	36.0	37.0	31.0	37.0
4	35.16708446549656	37.0	36.0	37.0	30.0	37.0
5	35.13937848737126	37.0	36.0	37.0	30.0	37.0
6	35.1633971763993	37.0	36.0	37.0	30.0	37.0
7	35.17981673327777	37.0	36.0	37.0	30.0	37.0
8	35.13994924764128	37.0	36.0	37.0	30.0	37.0
9	35.14660145533681	37.0	36.0	37.0	30.0	37.0
10-11	35.066696307326296	37.0	36.0	37.0	30.0	37.0
12-13	35.09792104527731	37.0	36.0	37.0	30.0	37.0
14-15	35.112788320645095	37.0	36.0	37.0	30.0	37.0
16-17	35.05309213982686	37.0	36.0	37.0	30.0	37.5
18-19	35.00272612273292	37.0	35.5	37.0	30.0	38.0
20-21	35.00025486949771	37.0	35.0	37.0	30.0	38.0
22-23	34.94300106790482	37.0	35.0	37.0	30.0	38.0
24-25	34.964161761047535	37.0	35.0	37.0	30.0	38.0
26-27	34.84349380548438	37.0	35.0	37.0	29.5	38.0
28-29	34.888855471100285	37.0	35.0	37.0	29.5	38.0
30-31	34.80607786852232	37.0	35.0	37.0	29.0	38.0
32-33	34.7125921377892	37.0	35.0	37.0	29.0	38.0
34-35	34.67747876221195	37.0	35.0	37.0	29.0	38.0
36-37	34.66032129229316	37.0	35.0	37.0	29.0	38.0
38-39	34.660443020523175	37.0	35.0	37.0	29.0	38.0
40-41	34.51164669605457	37.0	35.0	37.0	28.0	38.0
42-43	34.48888154441789	37.0	35.0	37.0	28.0	38.0
44-45	34.44784773954221	37.0	35.0	37.0	28.0	38.0
46-47	34.4019474825982	37.0	35.0	37.0	27.5	38.0
48-49	34.415230264159035	37.0	35.0	37.0	28.0	38.0
50-51	34.32242561972686	37.0	35.0	37.0	27.5	38.0
52-53	34.16567966471118	37.0	34.0	37.0	27.0	38.0
54-55	34.11159364764846	37.0	34.0	37.0	26.5	38.0
56-57	34.09109030428958	37.0	34.0	37.0	26.5	38.0
58-59	33.98973724044065	37.0	34.0	37.0	26.0	38.0
60-61	33.868798417617555	37.0	34.0	37.0	26.0	38.0
62-63	33.830160963734784	37.0	34.0	37.0	25.5	38.0
64-65	33.80427517013881	37.0	34.0	37.0	25.0	38.0
66-67	33.762837962832506	37.0	34.0	37.0	25.0	38.0
68-69	33.622886997053186	37.0	33.5	37.0	25.0	38.0
70-71	33.50086584071413	37.0	33.0	37.0	24.0	38.0
72-73	33.41430024879275	37.0	33.0	37.0	24.0	38.0
74-75	33.36286881550869	37.0	33.0	37.0	24.0	38.0
76-77	33.34294870699099	37.0	33.0	37.0	23.5	38.0
78-79	33.26096245146063	37.0	33.0	37.0	23.0	38.0
80-81	33.14776781496799	36.0	33.0	37.0	23.0	38.0
82-83	33.08125722369225	36.0	33.0	37.0	22.5	38.0
84-85	32.91491246836803	36.0	32.0	37.0	22.0	38.0
86-87	32.77492280641246	36.0	32.0	37.0	21.5	37.5
88-89	32.69576278745179	36.0	32.0	37.0	21.0	37.0
90-91	32.58402611795461	36.0	32.0	37.0	20.5	37.0
92-93	32.47336411653437	36.0	31.0	37.0	20.0	37.0
94-95	32.416185682552225	36.0	31.0	37.0	20.0	37.0
96-97	32.37489557952027	36.0	31.0	37.0	20.0	37.0
98-99	32.59407582109587	36.0	31.5	37.0	21.0	37.0
100	34.25156501263109	36.0	33.0	37.0	27.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	63.0
20	4328.0
21	50681.0
22	187610.0
23	357373.0
24	478788.0
25	577586.0
26	695784.0
27	844887.0
28	1034544.0
29	1277097.0
30	1594532.0
31	2023065.0
32	2623189.0
33	3520808.0
34	5092459.0
35	9084281.0
36	1.6243297E7
37	441080.0
>>END_MODULE
>>Per base sequence content	pass
#Base	G	A	T	C
1	24.11193030254229	31.73151230694315	23.606769044967425	20.54978834554713
2	24.230326365240078	31.29954493932774	27.86432424040381	16.605804455028377
3	18.272435185992116	29.432860052288206	30.25420280916479	22.040501952554887
4	19.71994988298928	30.084639167429327	29.095589295090097	21.099821654491297
5	21.021916816029144	28.924615550129136	29.071486392410712	20.981981241431004
6	20.965630375490026	28.722842404853182	29.034138621798782	21.277388597858007
7	21.334101778242783	29.278650927727227	28.65925105330678	20.727996240723208
8	20.276651564403426	28.45573914200023	29.803946039970025	21.463663253626315
9	20.56550289607478	28.10391856770089	29.924545954139326	21.406032582085004
10-11	21.051048541232376	28.768376032524852	29.125646099484253	21.054929326758526
12-13	20.989835974260636	28.884081187153974	28.96050856248068	21.165574276104714
14-15	21.000706780488105	28.83442621112585	28.923028076039927	21.24183893234612
16-17	21.1919214047728	28.81413653659854	28.892635530622485	21.10130652800618
18-19	21.116339025489882	28.62137538626562	28.928079656313155	21.334205931931344
20-21	21.139526592395033	28.579758418805568	28.953952866211097	21.3267621225883
22-23	21.286714405640193	28.805211286173964	28.772648283812668	21.135426024373174
24-25	21.218665412591026	28.696383017039885	28.76517246103748	21.319779109331606
26-27	21.232347800719726	28.581963105443098	28.822179854283014	21.363509239554162
28-29	21.369195817555813	28.708690864404318	28.70719361695985	21.214919701080024
30-31	21.321266205458908	28.606818569396715	28.716020540007147	21.355894685137226
32-33	21.311161141522696	28.560477329560484	28.73591937565685	21.39244215325997
34-35	21.454135777183392	28.694497753643766	28.61218700290377	21.239179466269068
36-37	21.411920283654613	28.5935874140561	28.603420304665484	21.391071997623808
38-39	21.377638256367224	28.520973148396596	28.68525955150834	21.416129043727842
40-41	21.498748739382993	28.63424982053037	28.59098309120853	21.276018348878107
42-43	21.432351322375183	28.539415640313358	28.619123012471153	21.409110024840302
44-45	21.395144696533908	28.506491368893528	28.659747666612233	21.438616267960334
46-47	21.529512336207016	28.600605024966804	28.581455673381928	21.288426965444256
48-49	21.471248729131162	28.528425514774035	28.5702783976629	21.430047358431903
50-51	21.444945464822943	28.452808429338532	28.63793209927165	21.464314006566877
52-53	21.572815683342732	28.57170615841072	28.539723519367037	21.315754638879508
54-55	21.512867512522917	28.48092207694452	28.55804471569401	21.448165694838554
56-57	21.50276634481871	28.418654951089284	28.603913388685704	21.474665315406302
58-59	21.63757436521352	28.52342481449802	28.48817926504445	21.350821555244007
60-61	21.60222437921546	28.422379570389666	28.500774975616714	21.47462107477816
62-63	21.53362285538981	28.388873086136595	28.57954081121309	21.4979632472605
64-65	21.664152160132353	28.502224316412878	28.49242130162163	21.341202221833143
66-67	21.62406829477416	28.41558106580794	28.495899939770876	21.464450699647024
68-69	21.602269660662785	28.376742183570254	28.529630145336004	21.49135801043096
70-71	21.76015303764189	28.45194712180305	28.409844201190836	21.378055639364224
72-73	21.69920658254454	28.36384120338292	28.447727074181067	21.489225139891477
74-75	21.676814038649425	28.31328647841848	28.490161214746173	21.51973826818592
76-77	21.77752200471261	28.442921634907226	28.41177838289713	21.36777797748304
78-79	21.73790382658347	28.35312660353001	28.407540008834587	21.50142956105193
80-81	21.72443654518771	28.30247069585392	28.445710541618357	21.527382217340012
82-83	21.850066268547717	28.414392763924873	28.345879836552797	21.389661130974616
84-85	21.81909256281714	28.323368946432648	28.330873314977463	21.52666517577275
86-87	21.830048224530927	28.221372590379456	28.37893919909992	21.569639985989696
88-89	21.956952302644257	28.360567717927452	28.251607121631437	21.43087285779685
90-91	21.935765997954597	28.247768226369534	28.271112627284534	21.54535314839133
92-93	21.910506762716725	28.20889603264031	28.28863635875583	21.591960845887133
94-95	22.015127379559683	28.31735518094267	28.211812825550425	21.455704613947223
96-97	21.982011830729025	28.233919829850308	28.218612851978463	21.5654554874422
98-99	21.90823086832951	28.150426754972475	28.300895130036025	21.64044724666199
100	22.217085515694674	27.78984437977632	28.14019822124429	21.85287188328472
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	21.0
1	14.5
2	9.0
3	11.5
4	13.5
5	27.0
6	71.5
7	173.0
8	461.0
9	1247.0
10	2727.0
11	5075.0
12	8833.0
13	14554.0
14	22784.0
15	34059.5
16	49126.0
17	68795.0
18	94973.5
19	126990.0
20	164295.0
21	208857.0
22	261092.0
23	321480.0
24	389701.0
25	462291.5
26	535569.0
27	612010.0
28	689727.5
29	768520.0
30	849001.0
31	924481.5
32	1000884.0
33	1081476.0
34	1162119.5
35	1247328.5
36	1337479.0
37	1427221.0
38	1513586.5
39	1595964.5
40	1667106.5
41	1718588.5
42	1750392.0
43	1764966.5
44	1758766.0
45	1727010.5
46	1675293.5
47	1608207.5
48	1524013.0
49	1428199.0
50	1326258.0
51	1219910.5
52	1113349.5
53	1008477.0
54	911421.5
55	822883.0
56	742282.5
57	673222.5
58	611788.0
59	556099.0
60	507632.5
61	463216.0
62	418595.0
63	374619.5
64	331224.5
65	289211.5
66	249581.0
67	211639.5
68	175481.5
69	141757.0
70	112088.5
71	87161.0
72	66068.0
73	48528.5
74	35084.5
75	24804.5
76	17317.0
77	11979.0
78	8132.0
79	5353.0
80	3420.5
81	2200.5
82	1385.0
83	889.0
84	568.0
85	353.5
86	208.5
87	111.0
88	67.5
89	40.5
90	28.0
91	15.5
92	9.5
93	8.0
94	3.5
95	2.0
96	1.5
97	1.0
98	1.0
99	0.5
100	0.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.11037805616870677
2	0.12628043877743106
3	0.05683324253483286
4	4.53053157745826E-4
5	0.08066297154488006
6	0.06090638551762905
7	0.030501966424122097
8	0.05875384108872185
9	0.00272048666493307
10-11	0.18224767778824735
12-13	0.014345960755798453
14-15	0.054624337426014684
16-17	0.030999457810259257
18-19	0.07677950392716883
20-21	0.08274289740544044
22-23	0.04517308494863764
24-25	0.014399069858022246
26-27	0.16060409284320815
28-29	0.02924360585918692
30-31	0.04457371079496913
32-33	0.06296896963052452
34-35	0.04383451880075225
36-37	0.001217173914231011
38-39	0.01610181270687079
40-41	0.11558599976432564
42-43	0.033092389981568326
44-45	0.07263916167217109
46-47	0.03253311861937491
48-49	0.01731248346572746
50-51	0.016670838802125717
52-53	0.0986561186064553
54-55	0.15541457485448323
56-57	0.018613114540595863
58-59	0.06632351394445594
60-61	0.04545488834819247
62-63	0.1719922204044456
64-65	0.0060262580320346116
66-67	0.023634637935143806
68-69	0.1615254047006507
70-71	0.060156369275349536
72-73	0.05104328976918145
74-75	0.011435806162662838
76-77	0.023133908685983907
78-79	0.05445530909620108
80-81	0.09611895937857047
82-83	0.010820196940807441
84-85	0.08259476319460121
86-87	0.03254748042138284
88-89	0.03208827571902667
90-91	0.0374027565724286
92-93	0.046974208176395804
94-95	0.03333386769332043
96-97	0.05988398220716401
98-99	0.039856731537559566
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
61	5.0
62	0.0
63	0.0
64	1.0
65	0.0
66	2.0
67	1.0
68	0.0
69	1.0
70	2.0
71	6.0
72	6.0
73	4.0
74	4.0
75	1.0
76	3.0
77	5.0
78	9.0
79	14.0
80	21.0
81	26.0
82	36.0
83	31.0
84	40.0
85	97.0
86	140.0
87	236.0
88	410.0
89	755.0
90	1286.0
91	2596.0
92	5062.0
93	10660.0
94	22462.0
95	51941.0
96	123385.0
97	315767.0
98	912014.0
99	3897372.0
100	4.0787051E7
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	79.68406398508948
#Duplication Level	Percentage of total
1	64.71978225747023
2	22.66483523089353
3	7.98312339681968
4	2.580714359513239
5	0.9193863700381829
6	0.3808139227002667
7	0.18005180881224936
8	0.14802411430689394
9	0.08366883782509892
>10	0.33190359099395955
>50	0.006463967692814019
>100	0.0012321429338418715
>500	0.0
>1k	0.0
>5k	0.0
>10k+	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	8.670873832456E-6	8.670873832456E-6	4.335436916228E-6	2.167718458114E-6	4.335436916228E-5	1.5174029206798E-5
2	8.670873832456E-6	1.083859229057E-5	1.5174029206798E-5	8.670873832456E-6	1.01882767531358E-4	2.167718458114E-5
3	8.670873832456E-6	1.3006310748684E-5	2.167718458114E-5	1.3006310748684E-5	1.14889078280042E-4	4.1186650704166E-5
4	1.3006310748684E-5	2.3844903039253998E-5	2.167718458114E-5	1.5174029206798E-5	1.36566262861182E-4	6.7199272201534E-5
5	1.9509466123026E-5	2.3844903039253998E-5	2.6012621497368E-5	2.167718458114E-5	1.45237136693638E-4	7.1534709117762E-5
6	1.9509466123026E-5	2.3844903039253998E-5	2.8180339955482E-5	3.251577687171E-5	1.69082039732892E-4	8.2373301408332E-5
7	2.167718458114E-5	2.8180339955482E-5	3.0348058413596E-5	3.9018932246052E-5	2.01597816604602E-4	8.670873832456E-5
8	2.8180339955482E-5	3.0348058413596E-5	3.0348058413596E-5	4.1186650704166E-5	2.21107282727628E-4	9.3211893698902E-5
9	3.0348058413596E-5	4.1186650704166E-5	3.251577687171E-5	4.335436916228E-5	2.3628131193442599E-4	9.9715049073244E-5
10-11	3.3599636100767E-5	4.7689806078508E-5	3.5767354558881E-5	4.6605946849450996E-5	2.6554551111896503E-4	1.1922451519627E-4
12-13	3.9018932246052E-5	5.6360679910964E-5	4.4438228391337E-5	5.8528398369078E-5	3.11067598739359E-4	1.4090169977740998E-4
14-15	5.0941383765679E-5	6.611541297247699E-5	4.8773665307564996E-5	6.0696116827192E-5	3.3924793869484103E-4	1.64746602816664E-4
16-17	5.7444539140021E-5	7.2618568346819E-5	5.3109102223793E-5	6.503155374342E-5	3.75015293253722E-4	1.94010802001203E-4
18-19	6.1779976056249E-5	7.8037864492104E-5	5.7444539140021E-5	7.587014603399E-5	4.3029211393562903E-4	2.23275001185742E-4
20-21	6.8283131430591E-5	8.670873832456E-5	6.0696116827191994E-5	8.8876456782674E-5	4.67143327723567E-4	2.35197452705369E-4
22-23	7.6954005263047E-5	9.6463471386073E-5	6.7199272201534E-5	9.6463471386073E-5	5.18084711489246E-4	2.55790778057452E-4
24-25	8.0205582950218E-5	1.083859229057E-4	7.6954005263047E-5	1.06218204447586E-4	5.60355221422469E-4	2.7855182186764895E-4
26-27	8.4541019866446E-5	1.12721359821928E-4	8.996031601173101E-5	1.14889078280042E-4	6.14548182875319E-4	2.9914514721973197E-4
28-29	8.670873832456E-5	1.17056796738156E-4	9.537961215701599E-5	1.24643811341555E-4	6.69825003557226E-4	3.17570754113701E-4
30-31	9.321189369890199E-5	1.3006310748684E-4	1.04050485989472E-4	1.36566262861182E-4	7.175148096357339E-4	3.6851213787938E-4
32-33	1.01882767531358E-4	1.37650122090239E-4	1.083859229057E-4	1.4090169977740998E-4	7.803786449210399E-4	4.1186650704165995E-4
34-35	1.06218204447586E-4	1.45237136693638E-4	1.12721359821928E-4	1.52824151297037E-4	8.399909025191749E-4	4.4980158005865497E-4
36-37	1.13805219050985E-4	1.54991869755151E-4	1.20308374425327E-4	1.6257888435855001E-4	9.19112626240336E-4	4.996591045952771E-4
38-39	1.24643811341555E-4	1.66914321274778E-4	1.23559952112498E-4	1.66914321274778E-4	9.80892602296585E-4	6.52483255892314E-4
40-41	1.3006310748684E-4	1.76669054336291E-4	1.25727670570612E-4	1.7341747664912E-4	0.001048091874498119	7.28353401926304E-4
42-43	1.36566262861182E-4	1.9943009814648801E-4	1.27895389028726E-4	1.78836772794405E-4	0.001119626583615881	7.771270672338689E-4
44-45	1.4957257360986598E-4	2.11352549666115E-4	1.33314685174011E-4	1.86423787397804E-4	0.001178154981984959	8.24816873312377E-4
46-47	1.52824151297037E-4	2.2327500118574198E-4	1.37650122090239E-4	1.9184308354308898E-4	0.001272450734912918	8.69255101703714E-4
48-49	1.5824344744232199E-4	2.40616748850654E-4	1.45237136693638E-4	1.9726237968837397E-4	0.001375417361673333	9.41873670050533E-4
50-51	1.66914321274778E-4	2.4928762268311E-4	1.53908010526094E-4	2.01597816604602E-4	0.001461042240768836	0.0010079890830230098
52-53	1.74501335878177E-4	2.59042355744623E-4	1.6366274358760698E-4	2.2219114195668501E-4	0.001562925008300194	0.0010643497629339738
54-55	1.78836772794405E-4	2.67713229577079E-4	1.71249758191006E-4	2.42784467308768E-4	0.001675646368122122	0.001153226219716648
56-57	1.89675365084975E-4	2.80719540325763E-4	1.75585195107234E-4	2.55790778057452E-4	0.001797038601776506	0.001229096365750638
58-59	2.0051395737554498E-4	2.87222695700105E-4	1.8642378739780398E-4	2.6554551111896503E-4	0.001932521005408631	0.0013071342302427421
60-61	2.03765535062716E-4	2.93725851074447E-4	1.92926942772146E-4	2.7530024418047794E-4	0.002060416394437357	0.001394926827796359
62-63	2.10268690437058E-4	3.02396724906903E-4	1.99430098146488E-4	2.8180339955481996E-4	0.002207821249589109	0.0014968095953277168
64-65	2.1677184581140002E-4	3.12151457968416E-4	2.11352549666115E-4	2.98061287990675E-4	0.002382322585467286	0.001611698673607759
66-67	2.21107282727628E-4	3.2732548717521396E-4	2.21107282727628E-4	3.04564443365017E-4	0.002554656202887349	0.0017298393295749719
68-69	2.2761043810197E-4	3.40331797923898E-4	2.29778156560084E-4	3.15403035655587E-4	0.002767092611782521	0.001869657170123325
70-71	2.3953288962159702E-4	3.4466723484012597E-4	2.3736517116348298E-4	3.32744783320499E-4	0.0029827805983648637	0.002020313602962248
72-73	2.4928762268311E-4	3.51170390214468E-4	2.47119904224996E-4	3.42499516382012E-4	0.003236403657964202	0.002170970035801171
74-75	2.5470691882839504E-4	3.5875740481786697E-4	2.5687463728650897E-4	3.4466723484012597E-4	0.0035171231982899648	0.0023313812017016072
76-77	2.6012621497368E-4	3.6851213787938E-4	2.63377792660851E-4	3.49002671756354E-4	0.0037739978355764738	0.002486373071456758
78-79	2.61210074202737E-4	3.77183011711836E-4	2.74216384951421E-4	3.62008982505038E-4	0.004051465798215066	0.0026684614219383337
80-81	2.7096480726424997E-4	3.78266870940893E-4	2.83971118012934E-4	3.65260560192209E-4	0.004345191649289513	0.002859220646252366
82-83	2.72048666493307E-4	3.82602307857121E-4	2.89390414158219E-4	3.6959599710843696E-4	0.004727793957146634	0.0030272188267562007
84-85	2.76384103409535E-4	3.93440900147691E-4	2.93725851074447E-4	3.80434589399007E-4	0.005134241168043009	0.00327325487175214
86-87	2.8180339955482E-4	4.03195633209204E-4	2.9697742876161803E-4	3.93440900147691E-4	0.005492998572860876	0.0034726849698986277
88-89	2.89390414158219E-4	4.1186650704166E-4	2.99145147219732E-4	4.06447210896375E-4	0.005857175273824028	0.003686205238022857
>>END_MODULE
