##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	SRR15674142
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	64086800
Total Bases	6.3 Gbp
Sequences flagged as poor quality	0
Sequence length	61-100
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	35.226510467054055	37.0	36.0	37.0	31.0	37.0
2	34.84945519202082	37.0	35.0	37.0	29.0	37.0
3	34.91225111879513	37.0	35.0	37.0	29.0	37.0
4	34.95602826167011	37.0	35.0	37.0	30.0	37.0
5	34.92779208823034	37.0	35.0	37.0	30.0	37.0
6	34.886153170387665	37.0	35.0	37.0	29.0	37.0
7	34.928881189262064	37.0	35.0	37.0	30.0	37.0
8	34.90024905284707	37.0	35.0	37.0	29.0	37.0
9	34.88939759825736	37.0	35.0	37.0	29.0	37.0
10-11	34.82878603706223	37.0	35.0	37.0	29.0	37.0
12-13	34.81742049064706	37.0	35.0	37.0	29.0	37.0
14-15	34.814577393472604	37.0	35.0	37.0	29.0	37.0
16-17	34.75928600741494	37.0	35.0	37.0	29.0	37.0
18-19	34.718934218903115	37.0	35.0	37.0	29.0	37.0
20-21	34.700961305604274	37.0	35.0	37.0	29.0	37.0
22-23	34.613648871530486	37.0	35.0	37.0	28.5	37.0
24-25	34.60629061678848	37.0	35.0	37.0	28.5	37.0
26-27	34.54393530336981	37.0	35.0	37.0	28.0	37.0
28-29	34.54919472496677	37.0	35.0	37.0	28.0	37.0
30-31	34.44916786296086	37.0	35.0	37.0	28.0	37.0
32-33	34.39172951372201	37.0	35.0	37.0	28.0	37.0
34-35	34.35797994282754	37.0	35.0	37.0	27.5	37.0
36-37	34.304945277342604	37.0	35.0	37.0	27.0	37.0
38-39	34.287930463059475	37.0	34.5	37.0	27.0	37.0
40-41	34.19297340481971	37.0	34.0	37.0	27.0	37.0
42-43	34.153858212611645	37.0	34.0	37.0	27.0	37.0
44-45	34.10802077806974	37.0	34.0	37.0	27.0	37.0
46-47	34.00356973666964	37.0	34.0	37.0	26.0	37.0
48-49	34.03118533769825	37.0	34.0	37.0	26.0	37.0
50-51	33.95233920245667	37.0	34.0	37.0	26.0	37.0
52-53	33.80414808509708	37.0	34.0	37.0	25.5	37.0
54-55	33.73697603094553	37.0	34.0	37.0	25.0	37.0
56-57	33.69799622543176	37.0	33.5	37.0	25.0	37.0
58-59	33.60122279470967	37.0	33.0	37.0	25.0	37.0
60-61	33.49741450657545	37.0	33.0	37.0	24.0	37.0
62-63	33.40554627329223	37.0	33.0	37.0	24.0	37.0
64-65	33.41408488106791	37.0	33.0	37.0	24.0	37.0
66-67	33.33286049799466	37.0	33.0	37.0	24.0	37.0
68-69	33.23148425332171	36.0	33.0	37.0	23.0	37.0
70-71	33.12539872349814	36.0	33.0	37.0	23.0	37.0
72-73	33.02557470419282	36.0	32.5	37.0	22.5	37.0
74-75	32.905084398437296	36.0	32.0	37.0	22.0	37.0
76-77	32.91706878189488	36.0	32.0	37.0	22.0	37.0
78-79	32.83071788735553	36.0	32.0	37.0	22.0	37.0
80-81	32.68949946586753	36.0	32.0	37.0	21.0	37.0
82-83	32.61624525377997	36.0	32.0	37.0	21.0	37.0
84-85	32.47715249806664	36.0	31.5	37.0	20.5	37.0
86-87	32.35509247755901	36.0	31.0	37.0	20.0	37.0
88-89	32.21711033626675	36.0	31.0	37.0	19.0	37.0
90-91	32.12936575045225	36.0	31.0	37.0	19.0	37.0
92-93	32.06592603084792	36.0	30.5	37.0	19.0	37.0
94-95	31.954407770403993	36.0	30.0	37.0	18.0	37.0
96-97	31.927798650480383	36.0	30.0	37.0	18.0	37.0
98-99	32.2462783733042	36.0	30.5	37.0	20.0	37.0
100	34.10090266530458	36.0	33.0	37.0	27.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	1.0
19	139.0
20	11686.0
21	129251.0
22	427822.0
23	721429.0
24	896885.0
25	1033797.0
26	1194290.0
27	1394372.0
28	1643219.0
29	1965048.0
30	2387756.0
31	2966932.0
32	3805220.0
33	5124867.0
34	7499758.0
35	1.2771226E7
36	1.9500755E7
37	612347.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	25.93275295975192	24.91855039169736	28.07041342412345	21.07828322442727
2	27.016551452826427	34.41856002550957	21.773507211005402	16.7913813106586
3	15.401241586286089	28.327760471732915	31.100332941789848	25.17066500019115
4	18.564256861879823	30.655696919921212	29.73318055747127	21.046865660727697
5	21.044762940861382	27.497442281804346	30.08279051551135	21.375004261822923
6	21.06673031011942	28.587055386418488	28.571241858070685	21.77497244539141
7	21.23931890077621	28.912257155950655	28.899111451886338	20.94931249138679
8	20.296877557745507	27.766362714827597	29.715183040356198	22.221576687070694
9	20.72801795160211	27.214428441814075	29.887323440374203	22.17023016620961
10-11	21.666386763753138	27.766070111539882	28.60937280552861	21.958170319178368
12-13	21.62171386832678	28.082657659733247	28.359273197860762	21.93635527407922
14-15	21.740627490748416	28.17327149422473	28.102466995664788	21.983634019362068
16-17	21.883384792000317	28.165564248180214	28.131443106152865	21.8196078536666
18-19	21.738763025669083	28.17206626323207	28.12655847358345	21.962612237515398
20-21	21.83943974145473	28.046529805142185	28.120568589196527	21.99346186420656
22-23	21.89477459108724	28.21201428195464	28.1223471746533	21.77086395230482
24-25	21.78626061393896	28.185001086533124	28.190435899226223	21.838302400301696
26-27	21.881142703565384	28.05463146869448	28.14261205463987	21.921613773100262
28-29	21.99447787464989	28.073950544438564	28.100660789039416	21.83091079187213
30-31	21.88810472330725	28.073082890129754	28.112868006471043	21.92594438009196
32-33	21.95234391348392	27.996741550898353	28.068313126427398	21.98260140919033
34-35	21.995554451645855	28.120102947470638	28.08780718918782	21.796535411695682
36-37	21.91713786962043	28.103335996994446	28.109461421485932	21.87006471189919
38-39	21.997729166801356	27.976835383770215	28.04718948551695	21.978245963911476
40-41	22.098962994593474	28.006555907223195	28.008928022139408	21.88555307604392
42-43	21.94096752918499	28.031278416885353	28.07393296022125	21.95382109370841
44-45	21.9951530298996	27.966175698189495	28.042331755261724	21.996339516649186
46-47	22.070675066004657	28.011516143836353	28.082358482653717	21.83545030750527
48-49	22.00203708274638	27.999159498344827	28.072647602140055	21.926155816768738
50-51	22.090052299513545	27.87570212749057	28.009455596865536	22.024789976130354
52-53	22.16050285285698	27.927185930503867	27.98931594520458	21.922995271434576
54-55	22.034324178727804	27.9619519817137	28.0411429288348	21.962580910723695
56-57	22.104674520997197	27.868932923267316	28.021396713838865	22.004995841896623
58-59	22.201923777369842	27.89763123471508	28.024573144859268	21.87587184305581
60-61	22.138371545019275	27.881277948912096	28.012741630328406	21.967608875740222
62-63	22.184004360660232	27.75260760072003	27.990865510738207	22.07252252788153
64-65	22.25135782244232	27.849267350040556	27.976296364913544	21.923078462603574
66-67	22.13425730520155	27.887719747407758	28.016510543167627	21.96151240422307
68-69	22.210785346116744	27.786975017648608	27.970775148840133	22.03146448739452
70-71	22.321888025932786	27.81268356192531	27.943229495653636	21.922198916488263
72-73	22.25270738229544	27.799624401903234	27.938989544180075	22.008678671621254
74-75	22.328150985515506	27.69420316226139	27.88993433697537	22.087711515247737
76-77	22.37184717300679	27.79185117309728	27.911917098971244	21.924384554924693
78-79	22.26867768097926	27.80138655690519	27.964363967292556	21.965571794823
80-81	22.377490454157805	27.672116107424078	27.88588050817201	22.064512930246106
82-83	22.480251311642157	27.722783140435997	27.841891597717833	21.955073950204014
84-85	22.395641301666068	27.706889311797383	27.859722999880127	22.03774638665642
86-87	22.486930730143754	27.5979180646465	27.808308638697948	22.1068425665118
88-89	22.593272813835828	27.656896497422018	27.755944510164664	21.99388617857749
90-91	22.520213908639256	27.65029460689575	27.794420438651695	22.0350710458133
92-93	22.60171121738099	27.56280363101454	27.713809106842703	22.121676044761767
94-95	22.688286723449984	27.605063774376276	27.678536264677593	22.028113237496147
96-97	22.578790558972837	27.668874130965314	27.693766378162277	22.058568931899572
98-99	22.556580072172327	27.526909774598874	27.727468438218157	22.189041715010646
100	22.73921135391776	27.141198140585587	27.72334191492102	22.396248590575635
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	24.0
1	13.5
2	4.0
3	3.5
4	3.0
5	4.0
6	6.0
7	8.5
8	16.0
9	31.0
10	69.5
11	150.5
12	295.5
13	611.0
14	1198.5
15	2278.0
16	4295.0
17	7780.0
18	13218.0
19	21709.5
20	35536.5
21	56756.0
22	87264.0
23	129005.0
24	183202.0
25	248508.5
26	325073.5
27	412040.5
28	507703.0
29	614691.0
30	734713.0
31	869115.5
32	1018080.0
33	1182219.0
34	1362086.0
35	1558696.5
36	1776222.0
37	2004442.0
38	2236537.5
39	2462934.0
40	2668420.0
41	2853957.5
42	3005158.5
43	3110001.0
44	3160156.0
45	3153197.5
46	3089507.0
47	2964086.5
48	2792614.5
49	2592016.5
50	2372023.5
51	2141513.0
52	1909608.0
53	1682793.0
54	1466947.0
55	1263445.5
56	1073228.0
57	901979.5
58	751830.5
59	624242.5
60	517456.0
61	426779.5
62	350437.5
63	288193.0
64	236531.5
65	192829.5
66	156946.0
67	127180.5
68	102750.0
69	82501.5
70	64900.0
71	50413.5
72	39100.0
73	29704.5
74	22169.5
75	16356.5
76	11757.5
77	8285.5
78	5731.5
79	3960.0
80	2700.0
81	1789.5
82	1194.5
83	776.5
84	486.0
85	321.0
86	218.0
87	124.0
88	67.5
89	48.0
90	28.0
91	19.0
92	15.0
93	7.5
94	5.5
95	3.5
96	1.5
97	2.0
98	2.0
99	1.0
100	0.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.07461442918042405
2	0.09461854859347012
3	0.04158890754414326
4	0.006633191234388361
5	0.046349638303051485
6	0.07286367863585012
7	0.05532652589924915
8	0.03559703402260684
9	0.0018022432076496254
10-11	0.11938495914915397
12-13	0.01666723880736751
14-15	0.059144004693634254
16-17	0.04183466798155002
18-19	0.047956053352640485
20-21	0.05708741893806525
22-23	0.0892367851101943
24-25	0.07170665410037637
26-27	0.1045371277704613
28-29	0.02185473451631225
30-31	0.06278359974284876
32-33	0.046726470973741864
34-35	0.03052578690151482
36-37	0.0022555346810887735
38-39	0.08138025303182558
40-41	0.07976135491240005
42-43	0.023529026258137402
44-45	0.050157754795059205
46-47	0.046448722669878977
48-49	0.01310956390395526
50-51	0.012037580281742887
52-53	0.06439001479243776
54-55	0.10432101462391631
56-57	0.01859041175405856
58-59	0.05348059194717165
60-61	0.051473158279084
62-63	0.12336082362924788
64-65	0.00960962409821905
66-67	0.0222799418638399
68-69	0.10875330327440808
70-71	0.045051409936162426
72-73	0.04100924002629466
74-75	0.10504821736895083
76-77	0.024483223448669357
78-79	0.04871367894273013
80-81	0.07848120272911298
82-83	0.021090201775621476
84-85	0.06662246078522471
86-87	0.04247639994117296
88-89	0.04361064869115663
90-91	0.051308106250872856
92-93	0.049261547351977616
94-95	0.04891761423069965
96-97	0.062400175661535794
98-99	0.0510809599724969
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
61	3.0
62	1.0
63	1.0
64	2.0
65	1.0
66	0.0
67	2.0
68	2.0
69	2.0
70	3.0
71	4.0
72	5.0
73	8.0
74	10.0
75	10.0
76	10.0
77	5.0
78	18.0
79	19.0
80	21.0
81	19.0
82	44.0
83	62.0
84	106.0
85	140.0
86	254.0
87	427.0
88	711.0
89	1299.0
90	2289.0
91	4577.0
92	8916.0
93	18458.0
94	38531.0
95	86315.0
96	203576.0
97	508426.0
98	1464591.0
99	5886474.0
100	5.5861458E7
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	67.80685821798129
#Duplication Level	Percentage of total
1	52.1148019484729
2	18.21008129747122
3	9.570274086927055
4	5.592699553468772
5	3.739730682785843
6	2.4633545558977823
7	1.8381738377575891
8	1.3054003277442485
9	0.8994200517425303
>10	4.230077949834914
>50	0.031438809621761044
>100	0.004546898275450018
>500	0.0
>1k	0.0
>5k	0.0
>10k+	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	7.801918647833874E-6	1.560383729566775E-6	1.560383729566775E-6	1.0922686106967425E-5	2.808690713220195E-5	3.74492095096026E-5
2	9.36230237740065E-6	1.560383729566775E-6	4.681151188700325E-6	2.0284988484368076E-5	4.99322793461368E-5	4.525112815743648E-5
3	9.36230237740065E-6	3.12076745913355E-6	6.2415349182671E-6	2.6526523402635177E-5	5.61738142644039E-5	5.4613430534837125E-5
4	1.7164221025234526E-5	6.2415349182671E-6	9.36230237740065E-6	4.213036069830293E-5	6.397573291223777E-5	6.397573291223777E-5
5	2.184537221393485E-5	9.36230237740065E-6	1.0922686106967425E-5	5.4613430534837125E-5	7.177765156007166E-5	7.333803528963843E-5
6	2.49661396730684E-5	9.36230237740065E-6	1.24830698365342E-5	6.2415349182671E-5	7.957957020790553E-5	7.801918647833875E-5
7	2.49661396730684E-5	1.24830698365342E-5	1.4043453566100975E-5	7.48984190192052E-5	8.894187258530618E-5	8.582110512617262E-5
8	2.808690713220195E-5	1.4043453566100975E-5	1.4043453566100975E-5	8.11399539374723E-5	1.0142494242184038E-4	8.894187258530618E-5
9	2.808690713220195E-5	1.560383729566775E-5	1.87246047548013E-5	8.582110512617262E-5	1.0766647734010747E-4	9.518340750357328E-5
10-11	3.0427482726552113E-5	1.9504796619584688E-5	2.106518034915146E-5	9.986455869227362E-5	1.2405050650055862E-4	1.0532590174575731E-4
12-13	3.666901764481921E-5	2.6526523402635177E-5	2.808690713220195E-5	1.1468820412315796E-4	1.4433549498492668E-4	1.2249012277099183E-4
14-15	4.36907444278697E-5	2.808690713220195E-5	3.1987866456118886E-5	1.295118495540423E-4	1.552581810918941E-4	1.4433549498492668E-4
16-17	4.915208748135341E-5	3.2768058320902275E-5	3.2768058320902275E-5	1.583789485510277E-4	1.6930163465799511E-4	1.552581810918941E-4
18-19	5.851438985875406E-5	3.588882578003583E-5	3.354825018568566E-5	1.7008182652277848E-4	1.8880643127757978E-4	1.7320259398191204E-4
20-21	6.631630850658795E-5	4.213036069830293E-5	3.666901764481921E-5	1.825648963593127E-4	2.067508441675977E-4	1.9036681500714654E-4
22-23	7.645880274877197E-5	4.6811511887003254E-5	3.9009593239169376E-5	2.005093092493306E-4	2.3483775129979966E-4	2.0441026857324755E-4
24-25	8.348052953182246E-5	4.915208748135341E-5	3.9789785103952764E-5	2.122121872210814E-4	2.5278216418981756E-4	2.2547544892239898E-4
26-27	9.362302377400651E-5	5.4613430534837125E-5	4.36907444278697E-5	2.262556407871824E-4	2.7072657707983547E-4	2.473208211363338E-4
28-29	9.98645586922736E-5	5.695400612918729E-5	4.6811511887003254E-5	2.4107928621806674E-4	2.9257194929377037E-4	2.691661933502687E-4
30-31	1.0922686106967425E-4	5.9294581723537456E-5	5.539362239962052E-5	2.6448504216156836E-4	3.3392211812728986E-4	3.073955947246547E-4
32-33	1.1468820412315796E-4	6.553611664180455E-5	5.9294581723537456E-5	2.816492631868029E-4	3.659099845834087E-4	3.331419262625065E-4
34-35	1.2249012277099183E-4	6.94370759657215E-5	5.9294581723537456E-5	2.98033292347254E-4	4.135016883351954E-4	3.620090252594918E-4
36-37	1.373137682018762E-4	8.035976207268892E-5	6.007477358832084E-5	3.097361703190049E-4	4.5953300835741527E-4	4.174026476591123E-4
38-39	1.4043453566100973E-4	8.192014580225569E-5	6.631630850658795E-5	3.2846077507380617E-4	4.977624097318012E-4	5.344314273766205E-4
40-41	1.4433549498492668E-4	8.582110512617264E-5	7.099745969528826E-5	3.46405187963824E-4	5.33651235511837E-4	5.796825555340569E-4
42-43	1.505770299031938E-4	9.284283190922311E-5	7.48984190192052E-5	3.627892171242752E-4	5.874844741818909E-4	6.350761779336774E-4
44-45	1.5993933228059443E-4	9.830417496270682E-5	7.645880274877197E-5	3.768326706903762E-4	6.358563697984609E-4	6.740857711728468E-4
46-47	1.6149971601016122E-4	1.0298532615140716E-4	8.11399539374723E-5	3.846345893382101E-4	7.123151725472328E-4	7.201170911950666E-4
48-49	1.6774125092842832E-4	1.0766647734010749E-4	8.504091326138924E-5	3.947770835803941E-4	7.755107135946872E-4	7.622474518933696E-4
50-51	1.7710355330582896E-4	1.1546839598794136E-4	8.582110512617262E-5	4.0647996155214494E-4	8.441675976956252E-4	8.199816498873403E-4
52-53	1.8412528008887943E-4	1.2249012277099183E-4	9.362302377400651E-5	4.2598475817172963E-4	9.268679353626643E-4	8.784960397460943E-4
54-55	1.9504796619584686E-4	1.2639108209490878E-4	1.0064475055705698E-4	4.493905141152312E-4	0.0010259523021901545	9.307688946865813E-4
56-57	2.0284988484368076E-4	1.318524251483925E-4	1.0220513428662377E-4	4.7435665378829964E-4	0.0011125535991811106	9.838219414918517E-4
58-59	2.1533295468021497E-4	1.38874151931443E-4	1.0766647734010749E-4	4.899604910839673E-4	0.0011975945124424999	0.0010446769069449558
60-61	2.239150651928322E-4	1.4199491939057652E-4	1.1078724479924103E-4	5.079049039739853E-4	0.001310722332836091	0.0011187951340993778
62-63	2.3015660011109932E-4	1.451156868497101E-4	1.1468820412315796E-4	5.172672063513859E-4	0.0014246303450944657	0.001210077582279034
64-65	2.3561794316458303E-4	1.5135722176797719E-4	1.1702877971750813E-4	5.344314273766205E-4	0.0015510214271893745	0.0012997996467291237
66-67	2.426396699476335E-4	1.6071952414537785E-4	1.2327031463577523E-4	5.51595648401855E-4	0.0016766323174195	0.001401224589150964
68-69	2.473208211363338E-4	1.6774125092842832E-4	1.2717127395969215E-4	5.742212124805733E-4	0.001834231074105744	0.0015205939444628224
70-71	2.5356235605460093E-4	1.7164221025234527E-4	1.318524251483925E-4	5.945062009649413E-4	0.0019980713657102556	0.0016423038753690306
72-73	2.6058408283765146E-4	1.7944412890017912E-4	1.3497319260752605E-4	6.186921487732263E-4	0.0021665928085034675	0.0017671345737343728
74-75	2.683860014854853E-4	1.8958662314236317E-4	1.4043453566100973E-4	6.452186721758615E-4	0.0023733436526710647	0.001898206807017982
76-77	2.7540772826853584E-4	1.9738854179019704E-4	1.4589587871449347E-4	6.647234687954461E-4	0.002564490659542995	0.002023037505383324
78-79	2.785284957276693E-4	2.0128950111411397E-4	1.505770299031938E-4	6.7252538744328E-4	0.0027587584338740583	0.0021611314654499834
80-81	2.9179175742898695E-4	2.0753103603238107E-4	1.5447798922711074E-4	6.803273060911139E-4	0.0029826734990668906	0.002292983890598376
82-83	2.9959367607682085E-4	2.1455276281543155E-4	1.6149971601016122E-4	6.990519108459152E-4	0.0032073687561245063	0.0024583845659324538
84-85	3.136371296429218E-4	2.1533295468021495E-4	1.6462048346929477E-4	7.14655748141583E-4	0.0035046218566069766	0.0026206644738073987
86-87	3.269003913442394E-4	2.184537221393485E-4	1.7008182652277848E-4	7.333803528963843E-4	0.0037542832533376605	0.0028164926318680287
88-89	3.378230774512068E-4	2.2079429773369866E-4	1.7164221025234525E-4	7.552257251103192E-4	0.004003164458203561	0.0029811131153373235
>>END_MODULE
